Unveiling the new chapter in nanobody engineering: advances in traditional construction and AI-driven optimization by Jiwei Liu, Lei Wu, Anqi Xie, Weici Liu, Zhao He, Yuan Wan, Wenjun Mao

“…Different library types, including immune, naïve, and synthetic/semi-synthetic libraries, offer diverse options for various applications, while display platforms like phage display, cell surface display, and non-surface display provide efficient screening of target Nbs. …”
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Optical and electronic properties of hybrid organic-inorganic perovskite type [NH3-(CH2)3-COOH]2HgCl4 by Ouaaka El Mustafa, Khechoubi El Mostafa

“…[NH3-(CH2)3-COOH]2HgCl4 crystallizes in C2/c space group with cell parameters a=29.2762 (2) Å, b=11.0408 (1) Å, c=11.3944 (1) Å, β=112.532 (2) Å and Z=8.The atomic arrangement shows an alternation of organic and inorganic entities. …”
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Advancements in Predictive Analytics: Machine Learning Approaches to Estimating Length of Stay and Mortality in Sepsis by Houssem Ben Khalfallah, Mariem Jelassi, Jacques Demongeot, Narjès Bellamine Ben Saoud

“…Feature importance analysis identified ICU stay duration (ICU_DAYS_OBS) as the most influential predictor for both outcomes, alongside vital signs, white blood cell counts, and lactic acid levels. These findings highlight the potential of ML-driven clinical decision support systems (CDSSs) to enhance early risk assessment, optimize ICU resource planning, and support timely interventions. …”
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Loss of Cochlear Ribbon Synapse Is a Critical Contributor to Chronic Salicylate Sodium Treatment-Induced Tinnitus without Change Hearing Threshold by Wei Zhang, Zhe Peng, ShuKui Yu, Qing-Ling Song, Teng-Fei Qu, Lu He, Ke Liu, Shu-Sheng Gong

“…A immunostaining study showed that there is significant loss of anti-CtBP2 puncta (a marker of cochlear inner hair cell (HC) ribbon synapses) in treated animals in apical, middle, and basal turns (all p<0.05). …”
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The Relationship between Inflammatory Marker Levels and Hepatitis C Virus Severity by Qitian He, Quan He, Xue Qin, Shan Li, Taijie Li, Li Xie, Yan Deng, Yu He, Yongbin Chen, Zhifu Wei

“…Background. Red cell distribution width (RDW) and platelet-lymphocyte ratio (PLR) have been studied in a variety of etiological diseases. …”
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Direct Spinal Ventral Root Repair following Avulsion: Effectiveness of a New Heterologous Fibrin Sealant on Motoneuron Survival and Regeneration by Mateus Vidigal de Castro, Roberta Barbizan, Rui Seabra Ferreira, Benedito Barraviera, Alexandre Leite Rodrigues de Oliveira

“…Sciatic nerves were processed to investigate Schwann cell activity by p75NTR expression (4 weeks after surgery) and to count myelinated axons and morphometric evaluation (12 weeks after surgery). …”
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Autologous and Heterologous Minor and Major Bone Regeneration with Platelet-Derived Growth Factors by Gianna Dipalma, Angelo Michele Inchingolo, Valeria Colonna, Pierluigi Marotti, Claudio Carone, Laura Ferrante, Francesco Inchingolo, Andrea Palermo, Alessio Danilo Inchingolo

“…Conclusion: The findings of the reviewed studies underscore the potential of the CGF in enhancing bone regeneration through stimulating cell proliferation, angiogenesis, and extracellular matrix mineralization. …”
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Active Pedicle Epithelial Flap Transposition Combined with Amniotic Membrane Transplantation for Treatment of Nonhealing Corneal Ulcers by Ting Zhang, Yuexin Wang, Yanni Jia, Dongle Liu, Suxia Li, Weiyun Shi, Hua Gao

“…A significant decrease in inflammatory cell infiltration and corneal stromal edema was revealed 2 months postoperatively by confocal microscopy and AS-OCT. …”
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Inhibition of zinc dendrite growth by a preferential crystal surface modulation strategy by Weihua Zhou, Junrun Feng, Zhuo Chen, Ziming Wan, Haoyu Feng, Lin Sheng, Zhuo Peng, Wenyuan Zhang, Zhangxiang Hao

“…With the AS treatment of 20 minutes, the ratio between Zn (002) to Zn (100) is around 0.93, which exhibits the best electrochemical performance and enables the Zn//Zn symmetric battery to cycle over 2200 hours at 2 mA cm−2 and 1 mAh cm−2. The full cell AS-20//MnO2 had capacity retention of 41.4 % after 600 cycles under a current density of 0.5 A g−1, whereas that of bare Zn//MnO2 was less than 14.5 %.…”
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Glycosylated lysosomal membrane protein promotes tissue repair after spinal cord injury by reducing iron deposition and ferroptosis in microglia by Fangru Ouyang, Meige Zheng, Jianjian Li, Jinxin Huang, Jianan Ye, Jingwen Wang, Yuanzhe Zhao, Fangli Shan, Ziyu Li, Shuishen Yu, Fei Yao, Dasheng Tian, Li Cheng, Juehua Jing

“…Abstract Excessive iron deposition can lead to ferroptosis, a form of iron-dependent cell death detrimental to neuronal survival. Microglia have been identified as having a high capacity for iron deposition, yet it remains unclear whether microglia undergo ferroptosis while phagocytosing excessive amounts of iron after spinal cord injury (SCI). …”
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Isolation and partial characterization of a new strain of Klebsiella pneumoniae capable of high 1,3 propanediol production from glycerol by B. Sen, A.P. Dabir, V.B. Lanjekar, D.R. Ranade

“…Addition of glucose to the glycerol fermentation led to increased cell mass but no improvement in the 1,3 PDO production.…”
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Potential Role of ANGPTL4 in the Cross Talk between Metabolism and Cancer through PPAR Signaling Pathway by Laura La Paglia, Angela Listì, Stefano Caruso, Valeria Amodeo, Francesco Passiglia, Viviana Bazan, Daniele Fanale

“…Subsequently, ANGPTL4 has been shown to be involved in several nonmetabolic and metabolic conditions, both physiological and pathological, including angiogenesis and vascular permeability, cell differentiation, tumorigenesis, glucose homoeostasis, lipid metabolism, energy homeostasis, wound healing, inflammation, and redox regulation. …”
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Acid hydrolysis and microwave digestion enhanced protein extraction from red seaweed Palmaria palmata by Anushi Madushani Wijethunga, Quan Sophia He, Balakrishnan Prithiviraj, Xiaohong Sun

“…The protein yield increment can be attributed to effective cell wall disruption, as evidenced by SEM imaging and CLSM imaging. …”
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The Involvement of Notch1-RBP-Jk/Msx2 Signaling Pathway in Aortic Calcification of Diabetic Nephropathy Rats by Caipan Gong, Li Li, Chunmei Qin, Weihua Wu, Qi Liu, Ying Li, Linwang Gan, Santao Ou

“…This study explored the changes in expression of vascular smooth muscle cell (VSMC) markers and osteogenic markers, as well as the involvement of Notch1-RBP-Jk/Msx2 pathway in a rat model of diabetic nephropathy (DN) with vascular calcification. …”
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Macular Changes in a Mucopolysaccharidosis Type I Patient with Earlier Systemic Therapies by Augusto Magalhães, Ana Maria Cunha, Rodrigo Vilares-Morgado, Elisa Leão-Teles, Esmeralda Rodrigues, Manuel Falcão, Ângela Carneiro, Jorge Breda, Fernando Falcão-Reis

“…To describe retinal findings in a patient with mucopolysaccharidosis type I (MPS I) that underwent an early treatment with hematopoietic stem cell transplantation (HSCT) and enzyme replacement therapy (ERT). …”
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NIR‐II emissive biohybrid nanovesicles as mild‐temperature photothermal antibiofilm agents against acute bacterial skin and skin‐structure infections by Ji Wang, Zhihao Wu, Xiaoxi Ma, Zhihui Huang, Haorong Dong, Jinxin Zhang, Xiaoming Liu, Pengfei Zhang, Shuhuai Yao

“…Herein, we developed a biocompatible AIEgen‐based biohybrid nano formulation that incorporates the BPBBT (NIR‐II AIEgen) and antibiofilm α‐amylase into a red blood cell (RBC) membrane‐derived nanovesicle carrier for a PTT/biofilm degradation combination therapy. …”
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Virtual bronchoscopic navigation and guided radial endobronchial ultrasound for peripheral pulmonary lesions: harmonizing modalities to optimize accuracy by Parul Mrigpuri, Sidharth Raj Yadav, Divyendu Sharma, Sonam Spalgais, Vidushi Rathi, Nitin Goel, Balakrishnan Menon, Raj Kumar

“…The diagnostic yield of VBN-guided R-EBUS was 77.7% (95% confidence interval: 68.5-85.8%). Non-small cell carcinoma was the most frequent histopathological diagnosis (55.5%). …”
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A Chinese Traditional Therapy for Bleomycin-Induced Pulmonary Fibrosis in Mice by Lifang Sun, Minjie Mao, Zhisheng Yan, Cuiyun Zuo, Xiaojie Zhang

“…Levels of nitric oxide (NO), hydroxyproline (HYP), malondialdehyde (MDA), and total antioxidant capacity were measured to evaluate the degree of oxidation. A single-cell gel electrophoresis (SCGE) assay was used to evaluate bleomycin-induced DNA damage. …”
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Predicting the thickness of shallow landslides in Switzerland using machine learning by C. Schaller, C. Schaller, L. Dorren, M. Schwarz, C. Moos, A. C. Seijmonsbergen, E. E. van Loon

“…We explored 39 different covariates, including metrics on terrain, geomorphology, vegetation, and lithology, at three different cell sizes. To train the ML models, 21 variables were chosen based on the variable importance derived from RF models and expert judgement. …”
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Screening and evaluation of novel DPP-IV inhibitory peptides in goat milk based on molecular docking and molecular dynamics simulation by Kuo Dang, Jing Lan, Yanli Wang, Daodong Pan, Lihui Du, Shikun Suo, Yali Dang, Xinchang Gao

“…The inhibition of DPP-IV by GPFPLL and LPYPY after in vitro digestion reached 31.90 % ± 1.80 % and 39.37 % ± 0.90 %, respectively. In a Caco-2 cell experiment, GPFPLL and LPYPY exhibited significant inhibition of DPP-IV, reaching 46.53 % ± 3.48 % and 65.98 % ± 2.87 %, respectively, when the concentration of each peptide was 2 mg/mL. …”
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