Serotonin-Exacerbated DSS-Induced Colitis Is Associated with Increase in MMP-3 and MMP-9 Expression in the Mouse Colon by Menglu Chen, Lei Gao, Pan Chen, Dandan Feng, Yalin Jiang, Yongchao Chang, Jianjun Jin, Fong-Fong Chu, Qiang Gao

“…MMPs and tissue inhibitors of MMPs (TIMPs) mRNA were measured by real-time quantitative RT-PCR in mouse colon and in human Caco-2 cells and neutrophils. MMP-3 and MMP-9 protein levels were quantified from immunohistochemistry (IHC) images of mouse colons. …”
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Preservation of cellular structure via immersion fixation in brain banking by Macy Garrood, Emma L. Thorn, Adam Goldstein, Allison Sowa, William Janssen, Alyssa Wilson, Claudia S. López, Raakhee Shankar, Erin S. Stempinski, Kurt Farrell, John F. Crary, Andrew T. McKenzie

“…Additionally, we did not identify any significant number of ghost cells – a state of late-stage cellular necrosis – in the light micrographs analyzed. …”
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A novel de novo GABRA2 gene missense variant causing developmental epileptic encephalopathy in a Chinese patient by Li Yang, Xingyu Wan, Ran Hua, Junhong Jiang, Baotian Wang, Rui Tao, De Wu

“…A mutant plasmid was constructed and transfected into 293 T cells. Western blotting (WB) was used to evaluate mutant protein expression, while co‐immunoprecipitation (Co‐IP) analyzed interactions with GABRB3 and GABRG2 proteins. …”
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Molecular mobility and dielectric properties of dendronized side chain liquid crystalline polyamines with benzoate groups as lateral spacers by Xavier Montané, Robert Graf, Borja Pascual-José, Roberto Teruel-Juanes, Jordi Guardià, Marta Giamberini, José Antonio Reina, Amparo Ribes-Greus

“…Dendronized polymers are suitable candidates for the preparation of biomimetic membranes for ion transport in fuel cells, which is nowadays a sustainable way of producing electrical energy. …”
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Black Chokeberry (<i>Aronia melanocarpa</i>) Juice Supplementation Affects Age-Related Myocardial Remodeling in Rats by Elena Daskalova, Mina Pencheva, Slavi Delchev, Lyudmila Vladimirova-Kitova, Spas Kitov, Stoyan Markov, David Baruh, Petko Denev

“…The mean number of CD34+ cells in the myocardium increased by 18.6% in the AMJ group, as compared to CO (<i>p</i> < 0.05). …”
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Optimization of a microarray for fission yeast by Dong-Uk Kim, Minho Lee, Sangjo Han, Miyoung Nam, Sol Lee, Jaewoong Lee, Jihye Woo, Dongsup Kim, Kwang-Lae Hoe

“…Activation of frozen pooled cells for >1 day showed better resolution of intensity than no activation. …”
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Upregulation of Cardiac IL-10 and Downregulation of IFN-γ in Balb/c IL-4−/− in Acute Chagasic Myocarditis due to Colombian Strain of Trypanosoma cruzi by Marcos Vinicius da Silva, Vera Lúcia de Almeida, Wendyson Duarte de Oliveira, Natália Carasek Matos Cascudo, Pollyana Guimarães de Oliveira, Crislaine Aparecida da Silva, Aline Cristina Souza da Silva, Maria Luíza Gonçalves dos Reis Monteiro, Rosana Rosa Miranda Correa, Milton Adriano Pelli Oliveira, Ruy de Sousa Lino-Júnior, Mara Rúbia Nunes Celes, Juliana Reis Machado

“…The spleen was used to culture splenic cells in 48 h. Subsequently, cytokines TNF-α, IL-12p70, IL-10, IFN-γ, and IL-17 were measured in the blood, culture supernatant, and heart apex by ELISA. …”
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The Influence of Gastric Microbiota and Probiotics in <i>Helicobacter pylori</i> Infection and Associated Diseases by Jagriti Verma, Md Tanveer Anwar, Bodo Linz, Steffen Backert, Suneesh Kumar Pachathundikandi

“…This microbiota comprises several trillion microorganisms, which is equivalent to almost ten times the total number of cells of the human host. <i>Helicobacter pylori</i> is a known pathogen that colonizes the gastric mucosa of almost half of the world population. …”
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A Cause of Diarrhea in a Patient with Selective Immunoglobulin A Deficiency by M. I. Gonik, V. S. Shcherbakha, E. Yu. Yuryeva, I. M. Kartavenko, A. S. Tertychny, P. V. Pavlov, A. E. Leonovich, T. L. Lapina, V. T. Ivashkin

“…Despite the absence of antibodies to parietal cells and intrinsic factor, a diagnosis of autoimmune gastritis was established on the basis of histologically confirmed atrophy of the stomach; decrease in serum pepsinogen I and the ratio of pepsinogen I to pepsinogen II, hypergastrinemia; vitamin B12 deficiency; proven autoimmune thyroiditis (antibodies to thyroglobulin and thyroid peroxidase in the diagnostic titre). …”
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Association between P2X7 Receptor Polymorphisms and Bone Status in Mice by Susanne Syberg, Peter Schwarz, Solveig Petersen, Thomas H. Steinberg, Jens-Erik Beck Jensen, Jenni Teilmann, Niklas Rye Jørgensen

“…Because P2X7 receptors are expressed in bone cells and are implicated in bone physiology, we asked whether strains with the P451L mutation have a different bone phenotype. …”
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Encoding of cerebellar dentate neuron activity during visual attention in rhesus macaques by Nico A Flierman, Sue Ann Koay, Willem S van Hoogstraten, Tom JH Ruigrok, Pieter Roelfsema, Aleksandra Badura, Chris I De Zeeuw

“…Labeling with the retrograde tracer Cholera Toxin B in the recording location in the DN indicated that these neurons predominantly receive inputs from Purkinje cells in the D1 and D2 zones of the lateral cerebellum as well as neurons of the principal olive and medial pons, all regions known to connect with neurons in the prefrontal cortex contributing to planning of saccades. …”
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Gonadal Histology and Expression Analysis of Sex Characteristic Genes in Grass Carp at Different Ages by Bo QI, Shengjie LI, Jinxing DU, Xiaofang XIAO, Dongyun ZHANG, Tao ZHU, Caixia LEI, Zhijun LIU, Hongmei SONG

“…At 2 months of age, primitive germ cells were first observed in the reproductive crest, marking the formation of their primitive gonads. …”
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Enhancing the Therapeutic Effect and Bioavailability of Irradiated Silver Nanoparticle-Capped Chitosan-Coated Rosuvastatin Calcium Nanovesicles for the Treatment of Liver Cancer by Tamer Mohamed Mahmoud, Mohamed Mahmoud Abdelfatah, Mahmoud Mohamed Omar, Omiya Ali Hasan, Saad M. Wali, Mohamed S. El-Mofty, Mohamed G. Ewees, Amel E. Salem, Tarek I. Abd-El-Galil, Dina Mohamed Mahmoud

“…The irradiated chitosan-coated optimized formulation capped with silver nanoparticles (INops) revealed the highest inhibition effect on carcinoma cells (97.12%) compared to the chitosan-coated optimized formulation (CS.Nop; 81.64) and chitosan-coated optimized formulation capped with silver nanoparticles (CS.Nop.AgNPs; 92.41). …”
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hsa_circ_0008305 facilitates the malignant progression of hepatocellular carcinoma by regulating AKR1C3 expression and sponging miR-379-5p by Shenglan Huang, Kan Liu, Yongkan Xu, Hua Wang, Shumin Fu, Jianbing Wu

“…Functionally, circPKT2 promoted the HCC cells proliferation, migration, and invasion both in vitro and vivo. …”
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Interleukin 6 Function in the Skin and Isolated Keratinocytes Is Modulated by Hyperglycemia by Eric G. Lee, Lerin R. Luckett-Chastain, Kaitlin N. Calhoun, Benjamin Frempah, Anja Bastian, Randle M. Gallucci

“…Primary mouse keratinocytes cultured in high glucose for 7 days displayed 2-fold higher IL-6Rα mRNA and higher rmIL-6-induced nuclear pSTAT3, but lower SOCS3 basal levels compared to normal glucose-cultured cells. Thus, it appears that delayed diabetic skin wound healing is associated with increased induction and expression of IL-6 and its receptor, but its function in epidermal keratinocytes may be impaired.…”
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Serial Paediatrics Omics Tracking in Myalgic Encephalomyelitis (SPOT-ME): protocol paper for a multidisciplinary, observational study of clinical and biological markers of paediatr... by Adam Scheinberg, Natalie Thomas, Katherine Huang, Paul R Gooley, Elisha K Josev, Tracey Chau, Darcy Tantanis, Sarah J Knight, Christopher W Armstrong

“…ME/CFS patients aged 12–19 years and controls will donate biosamples of urine, blood, and peripheral blood mononuclear cells for an in-depth omics profiling analysis (whole-genome sequencing, metabolomics and quantitative proteomics) while being assessed by gold-standard clinical and neuropsychological measures. …”
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Can nebulised heparin reduce acute lung injury in patients with SARS‑CoV‑2 requiring advanced respiratory support in Ireland: the CHARTER‑Ireland phase Ib/IIa, randomised, parallel... by David Cosgrave, Bairbre McNicholas, Ciara Hanley, John Robert Sheehan, Padraig Calpin, Maeve Kernan, Darragh Murphy, Alberto Alvarez‑Iglesias, John Ferguson, Camilla Giacomini, Christine Greene, Catriona Cody, Shane McGeary, Marion Murphy, Marianne Fitzgerald, Gerard Curley, Barry Dixon, Roger J. Smith, Claire Masterson, Daniel O’Toole, Frank van Haren, John G. Laffey

“…Abstract Background Nebulised unfractionated heparin may attenuate COVID-19 ARDS by reducing pulmonary microvascular thrombosis, blocking SARS-CoV-2 entry into cells, and decreasing lung inflammation. COVID-19 patients with a raised d-dimer have areas of pulmonary hypoperfusion on CT perfusion scans of the lung and have increased mortality risk. …”
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Phenolic and Iridoid Glycosides from <i>Leonurus cardiaca</i> L. and Their Effects on the α, δ, and γ Subtypes of the PPAR System—Including the Discovery of the Novel Phenylethanoi... by Kenny Kuchta, Nobuyasu Matsuura, Tung Huu Nguyen, Christian Rusch, Munekazu Iinuma, Yukihiro Shoyama, Hans Wilhelm Rauwald

“…For the PPARδ experiment, all nineteen isolated constituents and GW0742 (positive control) were added to the medium of transfected COS-1 cells and further processed according to a standardized luciferase assay protocol. …”
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Nanonaringenin and Vitamin E Ameliorate Some Behavioral, Biochemical, and Brain Tissue Alterations Induced by Nicotine in Rats by Mohamed A. Kandeil, Eman T. Mohammed, Rania A. Radi, Fatma Khalil, Abdel-Razik H. Abdel-Razik, Mohamed M. Abdel-Daim, Ghada M. Safwat

“…Thickening and congestion of the meninges and degeneration of the cerebral neurons and glia cells were observed. Cosupplementation with NNG, vitamin E, and their combination with nicotine was beneficial in the alleviation of activity impairments and improved short memory and cognition defects and exploratory behaviors. …”
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Theranostic nanoemulsions suppress macrophage-mediated acute inflammation in rats by Riddhi Vichare, Yalcin Kulahci, Rebecca McCallin, Fatih Zor, Fatma Nurefsan Selek, Lu Liu, Caitlin Crelli, Anneliese Troidle, Michele Herneisey, James M. Nichols, Andrew J. Shepherd, Vijay S. Gorantla, Jelena M. Janjic

“…Macrophages are immune cells that contribute to the initiation, maintenance, and resolution of inflammation due to their phenotypic plasticity in response to signals from inflammatory microenvironments. …”
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