Reversal gene expression assessment for drug repurposing, a case study of glioblastoma by Shixue Sun, Zeenat Shyr, Kathleen McDaniel, Yuhong Fang, Dingyin Tao, Catherine Z. Chen, Wei Zheng, Qian Zhu

“…Among five prioritized candidates, in-vitro experiments validated Clofarabine and Ciclopirox as highly efficacious in selectively targeting GBM cancer cells. Conclusions The success of this study illustrated a promising avenue for accelerating drug development by uncovering underlying gene expression effect between drugs and diseases, which can be extended to other rare diseases and non-rare diseases.…”
Get full text

Targeting endoplasmic reticulum stress: an innovative therapeutic strategy for podocyte-related kidney diseases by Jiao Lv, Honghai Yu, Sasa Du, Pengyu Xu, Yunyun Zhao, Wenxiu Qi, Xiuge Wang

“…Podocytes, epithelial cells situated outside the glomerular basement membrane, are essential for maintaining the structural integrity and permeability of the glomerular filtration barrier. …”
Get full text

Major ozonated autohemotherapy promoted functional recovery following spinal cord injury in adult rats via the inhibition of oxidative stress and inflammation by Xia Liwei, Sun Yongming, Zhou Yue, Yang Qian, Huang Jianhan, Liu Dong

“…Activated astrocytes in MOA-treated rats exhibited larger soma and higher levels of extracellular matrix secretion, whereas reactive microglia in the MOA group presented with a ramified morphology in contrast to the amoeboid morphology exhibited by these cells in SCI model rats. MOA offers potential value as a means of protecting spinal cord integrity, potentially through anti-inflammatory, antioxidant, and regulatory effects that shape the polarization of astrocytes and microglia.…”
Get full text

Study on the mechanical properties of Ti-6Al-4V alloy with minimal surface structures: effects of different heat treatment temperatures by Kai Qian, Shuangyu Liu, Fulong Zhang, Guihua Hang, Bo Cui, Ziwei Guo, Qiushi Zhang, Wang Binhua

“…This study utilized selective laser melting (SLM) to fabricate TPMS scaffold models with porosities of 50%, 60%, 70%, and 80%, based on Gyroid and Primitive unit cells. Compression tests were conducted to investigate the changes in mechanical properties of TPMS scaffolds before and after heat treatment. …”
Get full text

Effects of a Pseudophysiological Environment on the Elastic and Viscoelastic Properties of Collagen Gels by Sébastien Meghezi, Frédéric Couet, Pascale Chevallier, Diego Mantovani

“…One approach to vascular tissue engineering is to culture vascular cells on a scaffold in a bioreactor. The bioreactor establishes pseudophysiological conditions for culture (medium culture, 37°C, mechanical stimulation). …”
Get full text

The gut microbiota‐derived metabolite indole‐3‐propionic acid enhances leptin sensitivity by targeting STAT3 against diet‐induced obesity by Zhiwei Wang, Shaying Yang, Liangju Liu, Aiqin Mao, Hao Kan, Fan Yu, Xin Ma, Lei Feng, Tingting Zhou

“…High‐fat diet (HFD), rather than genetic factors, induces excessive secretion of antimicrobial peptides by Paneth cells, inhibiting the growth of Clostridia in the gut and resulting in decreased production of the beneficial metabolite IPA. …”
Get full text

Leiomyosarcoma of the Right Gonadal Vein: Review of the Approach and Prognostic in a Rare Case by Noa de la Fuente, Manuel Rodríguez Blanco, Gemma Cerdán, Antonio Moral, Vicenç Artigas

“…It originates in the smooth muscle cells of the vessel wall and is frequently located in the inferior vena cava. …”
Get full text

Functional Characterization of Preadipocytes Derived from Human Periaortic Adipose Tissue by Diana Vargas, Jaime Camacho, Juan Duque, Marisol Carreño, Edward Acero, Máximo Pérez, Sergio Ramirez, Juan Umaña, Carlos Obando, Albert Guerrero, Néstor Sandoval, Gina Rodríguez, Fernando Lizcano

“…To analyze adipogenic capacity, adipokine expression, and the levels of thermogenic proteins, adipocyte precursor cells were isolated from periaortic and subcutaneous adipose tissues and induced to differentiation. …”
Get full text

The Role of PDGFs and PDGFRs in Colorectal Cancer by Roberta M. Manzat Saplacan, Loredana Balacescu, Claudia Gherman, Romeo I. Chira, Anca Craiu, Petru A. Mircea, Cosmin Lisencu, Ovidiu Balacescu

“…PDGFs/PDGFRs signaling pathway has been demonstrated to be involved in angiogenesis mainly by targeting pericytes and vascular smooth muscle cells. High levels of PDGF-BB were reported in CRC patients compared to those with adenomas, while elevated levels of PDGFR α/β in the stroma of CRC patients were correlated with invasion and metastasis. …”
Get full text

Research Progress on Immune Regulation Activities of Marine Sulfate Polysaccharides by Qiuyu CHEN, Ran ZHAO, Meishan MENG, Xinyu DOU, Biyi CHEN, Qiancheng ZHAO, Ying LI

“…The types of marine sulfated polysaccharides are summarized based on their structure and source, with a focus on functions involving regulating macrophages, natural killer cells, T/B lymphocytes, complement systems, and gut microbiota. …”
Get full text

Engineered tRNAs efficiently suppress CDKL5 premature termination codons by Stefano Pezzini, Aurora Mustaccia, Pierre Aboa, Giorgia Faustini, Alessio Branchini, Mirko Pinotti, Angelisa Frasca, Joseph J. Porter, John D. Lueck, Nicoletta Landsberger

“…In this study we explored if the recently developed Anticodon-edited tRNAs (ACE-tRNAs) offer an alternative readthrough strategy for CDD. Transfecting cells expressing different CDKL5 nonsense variants, we demonstrated that ACE-tRNAs efficiently restore full-length kinase synthesis. …”
Get full text

Radiofrequency Ablation Compared to Surgery for the Treatment of Benign Thyroid Nodules by Stella Bernardi, Chiara Dobrinja, Bruno Fabris, Gabriele Bazzocchi, Nicoletta Sabato, Veronica Ulcigrai, Massimo Giacca, Enrica Barro, Nicolò De Manzini, Fulvio Stacul

“…RFA, however, did not allow for any pathologic analysis of the nodules, which, in 6 patients who had undergone surgery (8%), revealed that the nodules harboured malignant cells. Conclusions. RFA might transform our approach to benign thyroid nodules.…”
Get full text

Cancer vaccines: platforms and current progress by Wanting Lei, Kexun Zhou, Ye Lei, Qiu Li, Hong Zhu

“…Preventive cancer vaccines, like those against HPV and HBV, reduce the incidence of virus-associated cancers, while therapeutic cancer vaccines aim to activate dendritic cells and cytotoxic T lymphocytes for durable anti-tumor immunity. …”
Get full text

Extraction, GC-MS analysis, cytotoxic, anti-inflammatory and anticancer potential of Cannabis sativa female flower; in vitro, in vivo and in silico by Waqas Safir, Arif Malik, Arif Malik, Haleema Saadia, Ayesha Zahid, Jinyao Li

“…The antiproliferative activities of the extract were determined on HeLa cells by using MTT, Crystal Violet, and Trypan Blue assays with an IC50 value suggesting 51%-77.6% lethality. …”
Get full text

Comprehensive chemical and bioactive investigation of Chinese peony flower: a case of valorization of by-products as a new food ingredient from Chinese herb by Meng-ling Peng, Ming-Jiong Gong, Jing Zhang, Anastassiya V. Gadetskaya, Qian-Wen Liang, Pei-Wen He, Xiao-Hui Qiu, Zhi-Hai Huang, Wen Xu, Wen Xu

“…The anti-inflammatory and antioxidant activities were analyzed using RAW264.7 cells, and DPPH, ABTS, FRAP, and ORAC antioxidant assays.ResultsA total of 150 chemical components were identified in CPF, among them, more than 50 components were reported from this species for the first time, with potential new chemicals reported. 67 quantified or semi-quantified targeted metabolomics analysis indicated a clear distinction between flower parts and four cultivars. …”
Get full text

Mogroside ⅡE, an in vivo metabolite of sweet agent, alleviates acute lung injury via Pla2g2a-EGFR inhibition by Weichao Lü, Guoqing Ren, Kuniyoshi Shimizu, Renshi Li, Chaofeng Zhang

“…A lipopolysaccharide (LPS)-induced ALI model was established in mice and MH-S cells were employed to explore the protective mechanism of M2E through the western blotting, co-immunoprecipitation, and quantitative real time-PCR analysis. …”
Get full text

CXCR6 Expression Is Important for Retention and Circulation of ILC Precursors by Sylvestre Chea, Cécilie Possot, Thibaut Perchet, Maxime Petit, Ana Cumano, Rachel Golub

“…Innate lymphoid cells are present at mucosal sites and represent the first immune barrier against infections, but what contributes to their circulation and homing is still unclear. …”
Get full text

Advances in Drug Targeting, Drug Delivery, and Nanotechnology Applications: Therapeutic Significance in Cancer Treatment by Fatih Ciftci, Ali Can Özarslan, İmran Cagri Kantarci, Aslihan Yelkenci, Ozlem Tavukcuoglu, Mansour Ghorbanpour

“…With drug targeting, conventional, biotechnological and gene-derived drugs target the body’s organs, tissues, and cells that can be selectively transported to specific regions. …”
Get full text

Study on the structure characterization and moisturizing effect of Tremella polysaccharide fermented from GCMCC5.39 by Meng Yang, Zilong Zhang, Yan He, Chengliang Li, Jinmei Wang, Xia Ma

“…The results showed that FTPS-2 has no cytotoxicity, and could significantly promote AQP3, TGM1, CASP14, HYAL2, FLG gene expression level in HaCaT cells. It has the most significant influence at HYAL2 protein expression on 50 μg/mL.…”
Get full text

Development and Characterization of an F-labeled Ghrelin Peptidomimetic for Imaging the Cardiac Growth Hormone Secretagogue Receptor by Ahmed Abbas MSc, Lihai Yu PhD, Tyler Lalonde BSc, Derek Wu BSc, Jonathan D. Thiessen PhD, Leonard G. Luyt PhD, Savita Dhanvantari PhD

“…In vitro analysis showed enhanced serum stability compared to natural ghrelin and significantly increased cellular uptake in GHSR1a-expressing OVCAR cells. Biodistribution studies in mice showed that tissue uptake of the tracer was independent of circulating ghrelin levels, and there was negligible cardiac uptake and high uptake in the liver, intestines, and kidneys. …”
Get full text