Dutasteride nanoemulsion preparation to inhibit 5‐alpha‐hair follicle reductase enzymes in the hair follicle; an ex vivo study by Mehri Memar Bashi Aval, Elham Hoveizi, Reza Mombeiny, Mostafa Kazemi, Saeedeh Saeedi, Shima Tavakol

“…Skin permeation analysis using Franz diffusion cells showed nanocarriers could pass through the skin and release dutasteride for 6 days. …”
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Rapid degradation of DHX36 revealing its transcriptional role by interacting with G‐quadruplex by Ziang Lu, Jinglei Xu, Yuqi Chen, Yuanyuan Zhou, Xiaolu Zhou, Qi Wang, Qi Wei, Shaoqing Han, Ruiqi Zhao, Xiaocheng Weng, Xiaolian Zhang, Xiang Zhou

“…First, we employ the cleavage under targets and tagmentation (CUT&Tag), an efficient method for mapping protein–DNA interactions, to identify the binding sites in the chromatin of MCF‐7 cells. Subsequently, we use the auxin‐inducible degron (AID) protein degradation system and improved nascent RNA sequencing method acrylonitrile‐mediated uridine‐to‐cytidine conversion sequencing (AMUC‐seq) to pinpoint genes directly regulated by DHX36. …”
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Impact of Temozolomide on Immune Response during Malignant Glioma Chemotherapy by Sadhak Sengupta, Jaclyn Marrinan, Caroline Frishman, Prakash Sampath

“…Although TMZ-induced lymphopenia has been attributed to facilitate antitumor vaccination studies by inducing passive immune response, in general lymphopenic conditions have been associated with poor immune surveillance leading to opportunistic infections in glioma patients, as well as disrupting active antiglioma immune response by depleting both T and NK cells. Deletion of O6-methylguanine-DNA-methyltransferase (MGMT) activity, a DNA repair enzyme, by temozolomide has been determined to be the cause of lymphopenia. …”
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Polymorphisms of Il-10 (-1082) and RANKL (-438) Genes and the Failure of Dental Implants by Rodrigo Ribeiro, Rayanne Melo, Pedro Tortamano Neto, André Vajgel, Paulo Roberto Eleutério Souza, Renata Cimões

“…An implant was considered a failure if any of the following occurred: mobility, persistent subjective complaint, recurrent peri-implant infection with suppuration, continuous radiolucency around the implant, probing depth ≥ 5 mm, and bleeding on probing. Buccal mucosal cells were collected for analysis of RANKL438 and IL-10. …”
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Unveiling nature’s antidiabetic potential in Berberis orthobotrys seeds for alloxan-induced diabetic rats by Ali Imran Abid, Hira Muzammel, Mohsin Javed, Muhammad Muntazir Iqbal, Sajid Mahmood, Ammar Zidan, Fadi Jaber, K. K. Mujeeb Rahman, Ali Bahadur, Shahid Iqbal, Muhammad Saad, Ayed A. Dera, Yousif A. Asiri, Abd-ElAziem Farouk, Salman Aloufi

“…The primary metabolite Berberine, which was qualitatively detected by GC-MS in blood and urine samples of rats, may be the alkaloid in command of insulin release or recovery of beta cells after alloxan destruction. More study is necessary to comprehend the underlying signaling process.…”
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Endometrial Cancer and Hypermethylation: Regulation of DNA and MicroRNA by Epigenetics by Kouji Banno, Iori Kisu, Megumi Yanokura, Kenta Masuda, Yusuke Kobayashi, Arisa Ueki, Kosuke Tsuji, Wataru Yamagami, Hiroyuki Nomura, Nobuyuki Susumu, Daisuke Aoki

“…Studies aimed at detection of aberrant DNA hypermethylation in cancer cells present in microscopic amounts in vivo and application of the results to cancer diagnosis have also started. …”
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Use of 5-Fluorouracil-Soaked Bioamniotic Membranes in Trabeculectomy for Primary Open-Angle Glaucoma: A Retrospective Analysis by Chenming Zhang, Min Wu, Jianrong Wang, Miaomiao Zhang, Xu Wang, Wei Liu

“…Preoperative and postoperative intraocular pressures (IOP), cup/disc ratio, visual acuity and postoperative macular OCT, complications, treatment, and number of corneal endothelial cells were measured, recorded, and analyzed. At the end of 2 years of follow-up, IOP of 36 (87.8%) eyes of patients in the 5-FU group and IOP of 28 (80%) eyes of patients in the MMC group were ≤21 mmHg and patients in the 5-FU group had more stable IOP than patients in the MMC group. …”
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Experimental Investigation of Particle Sorting in Y-Channels with Variable Bifurcation Angles for Microfluidic Applications by Khola Ikram, Syed Maaz Hasan, Ch Abdullah, M Zulfiqar, Emad Uddin, Zaib Ali, M. Sajid

“…To investigate the influence of flow-related parameters on systems performance, testing was conducted using 10 & 20 µm, 20 & 60 µm, and 40 & 60 µm sized particles and flow rates of 50-250 µL/min to stimulate red and white blood cells. Furthermore, imaging was done using a microscope, and postprocessing was done through MATLAB Image Processing. …”
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Exploring salinity induced adaptations in marine diatoms using advanced photonic techniques by Julijana Cvjetinovic, Yekaterina D. Bedoshvili, Nickolai A. Davidovich, Eugene G. Maksimov, Ekaterina S. Prikhozhdenko, Daria A. Todorenko, Daria V. Bodunova, Olga I. Davidovich, Igor S. Sergeev, Dmitry A. Gorin

“…Lipid droplets within the cells display a minimum diameter at 40‰, indicating metabolic adjustments to osmotic stress. …”
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Cardioprotective effects of hUCMSCs-Exosi-EGR1 MN patch in MI/RI by modulating oxidative stress and mitophagy by Chen Huang, Xun Zhang, Shi-Xiong Wu, Qing Chang, Zhi-Kun Zheng, Jing Xu

“…In an effort to address the detrimental effects of myocardial ischemia/reperfusion injury (MI/RI), this study introduces a novel therapeutic strategy that involves a microneedle (MN) patch loaded with exosomes derived from human umbilical cord mesenchymal stem cells (hUCMSCs) and carrying small interfering RNA (siRNA) targeting early growth response-1 (EGR1). …”
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Hemodynamic disturbance and mTORC1 activation: Unveiling the biomechanical pathogenesis of thoracic aortic aneurysms in Marfan syndrome by Ming-Yuan Liu, Meili Wang, Junjun Liu, An-Qiang Sun, Chang-Shun He, Xin Cong, Wei Kong, Wei Li

“…We uncovered that activation of the mTOR complex 1 (mTORC1) within smooth muscle cells, instigated by the oscillatory wall shear stress (OSS) that stems from disturbed flow (DF), is a catalyst for TAA progression. …”
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Prevalence and pathological studies on ovine pneumonic pasteurellosis in kashmir valley, india by Latief Mohammad Dar, Mohammad Maqbool Darzi, Masood Saleem Mir, Adil Rashid, Swaid Abdullah, Syed Ashiq Hussain

“…Duplicate sections were stained for connective tissue by Masson's Trichrome Stain, elastin by Hart's method, neutral and acid mucopolysaccharide by Combined Alcian Blue PAS technique, and mast cells by Toluidine Blue Stain.<p> <b>Results:</b> Out of 956 grossly pneumonic lung samples, Pasteurella spp. was isolated from 398 affected lung samples giving an overall prevalence of 18.46% (398/2155) in the population studied. …”
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A high-fidelity CRISPR-Cas13 system improves abnormalities associated with C9ORF72-linked ALS/FTD by Tristan X. McCallister, Colin K. W. Lim, Mayuri Singh, Sijia Zhang, Najah S. Ahsan, William M. Terpstra, Alisha Y. Xiong, M. Alejandra Zeballos C, Jackson E. Powell, Jenny Drnevich, Yifei Kang, Thomas Gaj

“…This high-fidelity system possessed improved transcriptome-wide specificity compared to its native form and mediated targeting in motor neuron-like cells derived from a patient with ALS. These results lay the foundation for the implementation of RNA-targeting CRISPR technologies for C9ORF72-linked ALS/FTD.…”
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Simulating Immune Interference on the Effect of a Bivalent Glycoconjugate Vaccine against Haemophilus influenzae Serotypes “a” and “b” by Angjelina Konini, Mingsong Kang, Seyed M. Moghadas

“…We compared simulation outcomes in the absence of any preexisting immunity with an already primed immune response having specific memory B cells and/or anti-Hib antibodies. Results. The simulation results show that the preexisting immune responses to Hib or carrier protein (CP) may significantly impede the production of anti-Hia antibodies by a unimolecular vaccine. …”
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Involvement of Stat1 in the Phagocytosis of M. avium by Sabrina Dominici, Giuditta Fiorella Schiavano, Mauro Magnani, Costantina Buondelmonte, Angela Gabriela Celeste, Giorgio Brandi

“…This activation enhances the survival of infected cells, but, at the same time, makes macrophages optimal targets for drugs development against p-tyr701stat1. …”
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Microplastic-enhanced chromium toxicity in Scenedesmus obliquus: Synergistic effects on algal growth and biochemical responses by Luoxin Li, Jiani Yu, Yonghua Ma, Hui Tan, Fengxia Tan, Yi Chai, Hui Zhang

“…A 30-day experimental simulation revealed that co-exposure to Cr6+ and 5 µm diameter polystyrene MPs resulted in significantly enhanced toxicity compared to individual exposures, characterized by a notable decrease in algal growth, diminished photosynthetic pigments, and protein content, alongside oxidative system damage. 100 nm MPs exhibited a distinct toxicity profile, with more pronounced effects when not combined with Cr6+, suggesting size-dependent interactions with algal cells. Transcriptomic analysis illuminated the complex regulatory mechanisms, indicating that toxicity primarily modulates metabolic pathways essential for photosynthesis, oxidative phosphorylation, the TCA cycle, and ribosome function in Scenedesmus obliquus. …”
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TOXICITY TEST OF LIME PEEL EXTRACT (Citrus aurantifolia) USING THE BRINE SHRIMP LETHALITY TEST (BSLT) METHOD FOR DETECTION OF ANTI CANCER ACTIVITY by Indah Khairunnisa, Endang Linirin Widiastuti, Nuning Nurcahyani

“…Based on phytochemical and FTIR tests, lime peel (C. aurantifolia) contains alkaloid, flavonoid, and tannin compounds which are known to have antioxidant properties and can disrupt the balance of Reactive Oxygen Species (ROS) so as to trigger apoptosis of cancer cells. …”
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Transcriptome analysis of testis in response to silencing of Piwi2 sheds light on spermatogenesis in the mud crab Scylla paramamosain by Tao Wang, Li Lu, Fang Liu, An Liu, Haihui Ye

“…In response to the prolonged down-regulation of Sp-Piwi2, the number of germ cells in the testis was markedly decreased. Transcriptome analysis further suggested that Sp-Piwi2 presumably regulates spermatogenesis by promoting spermatogonia proliferation and the differentiation of spermatogonia to spermatocytes, and is also involved in apoptosis and regulation of transposons. …”
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Virulence changes in Vibrio parahaemolyticus during the freezing of Penaeus chinensis by Zenghui Gao, Heer Ding, Ke Li, Qiang Zhang, Xubo Zhao, Liu Bin, Tianli Yue

“…These results demonstrated that the strains were not dead during freezing process, but became VBNC cells, which still produced and accumulated toxins, especially TDH, the most virulent factor.…”
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tRF-AspGTC Promotes Intracranial Aneurysm Formation by Controlling TRIM29-Mediated Galectin-3 Ubiquitination by Chao Wang, Bing Yu, Han Zhou, Huanting Li, Shifang Li, Xiaolu Li, Wentao Wang, Yugong Feng, Tao Yu

“…In this study, we identified that the transfer RNA-Asp-GTC derived fragment (tRF-AspGTC) is highly expressed in the IA tissues of both humans and mice. tRF-AspGTC promotes IA formation by facilitating the phenotypic switching of vascular smooth muscle cells, increasing of matrix metalloproteinase 9 expression, and inducing of oxidative stress and inflammatory responses. …”
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