Multi-gene precision editing tool using CRISPR-Cas12a/Cpf1 system in Ogataea polymorpha by Senqin Hou, Shibin Yang, Wenqin Bai

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Living Therapeutics for Synergistic Hydrogen‐Photothermal Cancer Treatment by Photosynthetic Bacteria by Yingyi Zhang, Xiaolian Deng, Lili Xia, Jianghui Liang, Meng Chen, Xiaoling Xu, Wei Chen, Jianwei Ding, Chengjie Yu, Limei Liu, Yang Xiang, Yiliang Lin, Fangfang Duan, Wei Feng, Yu Chen, Xiang Gao

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Engineering Saccharomyces cerevisiae for medical applications by Carla Maneira, Alexandre Chamas, Gerald Lackner

“…Abstract Background During the last decades, the advancements in synthetic biology opened the doors for a profusion of cost-effective, fast, and ecologically friendly medical applications priorly unimaginable. …”
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Design principles for engineering bacteria to maximise chemical production from batch cultures by Ahmad A. Mannan, Alexander P. S. Darlington, Reiko J. Tanaka, Declan G. Bates

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Proton motive force generated by microbial rhodopsin promotes extracellular electron transfer by Wenqi Ding, Tong Lin, Yun Yang, Wen-Wei Li, Shaoan Cheng, Hao Song

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Selective utilization of medicinal polysaccharides by human gut Bacteroides and Parabacteroides species by Zepeng Qu, Hongbin Liu, Ji Yang, Linggang Zheng, Jumin Huang, Ziming Wang, Chun Xie, Wenlong Zuo, Xiong Xia, Lin Sun, Yifa Zhou, Ying Xie, Jingguang Lu, Yizhun Zhu, Lili Yu, Lihua Liu, Hua Zhou, Lei Dai, Elaine Lai-Han Leung

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The manipulation of odor availability of training aids used in detection canine training by Katylynn B. Sloan, Michele N. Maughan, Caitlin E. Sharpes, Robin R. Greubel, Shawna F. Gallegos, Shawna F. Gallegos, Aleksandr E. Miklos, Lindsay D. Waldrop

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Nucleic acid therapeutics: Past, present, and future by Sajid Naeem, Ju Zhang, Yang Zhang, Yu Wang

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A design principle for neuronal firing with up-down oscillation through Na+ dynamics by Tomohide R. Sato, Koji L. Ode, Fukuaki L. Kinoshita, Hiroki R. Ueda

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Development of robust constitutive synthetic promoter using genetic resources of plant pararetroviruses by Tsheten Sherpa, Tsheten Sherpa, Nrisingha Dey

“…With the advancement of plant synthetic biology, complex genetic engineering circuits are being developed, which require more diverse genetic regulatory elements (promoters) to operate. …”
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Comparative transcriptome analyses of different orthosiphon aristatus tissues reveal differentially expressed genes associated with flavonoid biosynthesis by Qiaoxue Wang, Huan Long, Shumeng Liu, Xixi Xu, Qin Zhang, Shanyong Yi

“…However, there is limited knowledge on the mechanisms behind the synthetic biology of flavonoid in all tissues of O. aristatus. …”
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Poplar transformation with variable explant sources to maximize transformation efficiency by Haiwei Lu, Sara Jawdy, Jin-Gui Chen, Xiaohan Yang, Udaya C. Kalluri

“…The recent omnipresent emergence of synthetic biology, which relies on plant transformation to manipulate plant DNA and gene expression for novel product biosynthesis, has further propelled basic as well as applied interests in plant transformation technologies. …”
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Design of a humanized CD40 agonist antibody with specific properties using AlphaFold2 and development of an anti-PD-L1/CD40 bispecific antibody for cancer immunotherapy by Kun Du, He Huang

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Hydrophobic deep eutectic solvents as novel, sustainable aids for intracellular protein release from Saccharomyces cerevisiae by Tjalling Gijsbert Tjalsma, Yannick Patrice Didion, Ziran Su, Magdalena Malankowska, Pablo Torres-Montero, José Luis Martínez, Manuel Pinelo

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Fc-binding nanodisc restores antiviral efficacy of antibodies with reduced neutralizing effects against evolving SARS-CoV-2 variants by Jaehyeon Hwang, Soyun Choi, Beom Kyu Kim, Sumin Son, Jeong Hyeon Yoon, Kyung Won Kim, Wonbeom Park, Hyunjoo Choo, Suhyun Kim, Soomin Kim, Seokhyeon Yu, Sangwon Jung, Sang Taek Jung, Min-Suk Song, Sang Jick Kim, Dae-Hyuk Kweon

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Designer cellulosomes – catalytic nanomachines with significant potential in biotechnology and circular economy by Maša Vodovnik

“…The simultaneous advancements in synthetic biology, protein engineering, and the pursuit of greater sustainability across various industries have highlighted the immense potential of these artificially designed enzymatic complexes for diverse applications. …”
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Application of emerging technologies for gut microbiome research by Wit Thun Kwa, Saishreyas Sundarajoo, Kai Yee Toh, Jonathan Lee

“…As the emerging technologies for microbiome research continue to evolve (i.e. metatranscriptomics, metabolomics, culturomics, synthetic biology), microbiome research has moved beyond phylogenetic descriptions and towards mechanistic analyses. …”
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Vaccination ARN messager (ARNm), modèle de transition de la biologie fondamentale à la médecine by Sansonetti, Philippe J.

“…Sixty years of doubts for some and certainties for others about the possibility of using mRNA—an example of synthetic biology—in therapeutic medicine and vaccinology. …”
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AI, big data, and robots for the evolution of biotechnology by Haseong Kim

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