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81
Decoding the mannose receptor-mAb interaction: the importance of high-mannose N-glycans and glycan-pairing
Published 2024-12-01“…Two novel analytical methods, MR surface plasmon resonance and MR affinity chromatography, were developed and applied to investigate the MR-mAb interaction. …”
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82
Novel green synthesis of silver nanoparticles from empty fruit bunch waste: Biomedical applications and mechanistic insights
Published 2025-01-01“…EFB-AgNPs showed a surface plasmon resonance peak at 477 nm, hydroxyl and amine group stabilization, and a silver content of 75.56 %. …”
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83
Advance in identified targets of berberine
Published 2025-01-01“…Through target fishing, molecular docking, and surface plasmon resonance (SPR) analyses, combined with cellular and molecular experiments, we further identified 6 targets of berberine. …”
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84
Gallium Sulfide-Immobilized Optical Fiber-Based SPR Sensor for Detection of Brilliant Blue Food Adulteration
Published 2024-01-01“…Food safety assurance is crucial, particularly in identifying prohibited colors like brilliant blue (BB) that may pose significant health risks when used in food products. Surface plasmon resonance (SPR) biosensors provide a reliable, label-free method for highly sensitive detection of food adulterants. …”
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85
GENI as an AMPK Activator Binds α and γ Subunits and Improves the Memory Dysfunction of Alzheimer’s Disease Mouse Models via Autophagy and Neuroprotection
Published 2025-01-01“…Furthermore, the Western blot, small interfering RNA (siRNA), drug affinity responsive target stability (DARTS), cellular thermal shift assay (CESTA), liquid chromatography-tandem mass spectrometry (LC/MS-MS), adenosine 5′-monophosphate-activated protein kinase (AMPK) mutants and surface plasmon resonance (SPR) analysis were utilized to clarify the mechanism of action and identify target protein of this molecule. …”
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86
Domperidone inhibits dengue virus infection by targeting the viral envelope protein and nonstructural protein 1
Published 2025-01-01“…Molecular dynamics (MD) simulations and surface plasmon resonance (SPR) analysis were subsequently performed to determine specific interactions of domperidone with the DENV E and NS1 proteins and their binding affinity. …”
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87
Development of a stable eukaryotic strain producing fully human monoclonal antibody on the basis of the human antibody against ectromelia virus
Published 2018-01-01“…The binding affinity of purified antibody fh8E with the targeted protein, measured by surface plasmon resonance, was 12 nM. In addition, antibody fh8E demonstrated antivaccinia virus activity in the plaque reduction neutralization test in vitro.…”
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88
Full-length and N-terminally truncated recombinant interleukin-38 variants are similarly inefficient in antagonizing interleukin-36 and interleukin-1 receptors
Published 2025-01-01“…Binding of recombinant IL-38 proteins to the IL-36R was assessed by surface plasmon resonance. Results Analysis of its native N-terminus revealed that the IL-38 protein produced by human keratinocytes starts at cysteine 2. …”
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89
Bismuth-Immobilized Optical Fiber-Based SPR Nanosensor for Detection of Zinc Nitrate Contamination in Aquaculture Industry
Published 2024-01-01“…This study presents a bismuth-immobilized optical fiber-based surface plasmon resonance (SPR) biosensor for the rapid detection of zinc nitrate contamination. …”
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90
Development of a Recombinant Omicron BA.1 Subunit Vaccine Candidate in Pichia pastoris
Published 2025-01-01“…After in vitro characterisation of purified RBD‐Omicron via chromatography, mass spectrometry, calorimetry and surface plasmon resonance‐based methods, it was injected into mice for immunization studies. …”
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91
Eco-benign synthesis of multifunctional silver nanoparticles using leptochloa fusca for enhanced biomedical and catalytic applications
Published 2025-01-01“…These analyses confirmed the synthesis, chemical composition, size, morphology, crystalline nature and thermal stability of LF-AgNPs. The surface plasmon resonance of LF-AgNPs was recorded at 432–436 nm and the particle size measured via SEM analysis was noted to be 30 to 200 nm. …”
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92
Kaempferol protects against high-salt-induced hypertension and vascular endothelial injury by inhibiting ferroptosis through the ATF4/ACSL4 pathway
Published 2025-02-01“…Compared to ATF4+/−mice on a high-salt diet, ATF4+/−mice treated with Kae exhibited greater reductions in blood pressure and endothelial damage and more pronounced inhibition of ferroptosis. Surface plasmon resonance and molecular docking analyses revealed that Kae directly binds to ATF4, while proteome microarray and co-immunoprecipitation assays demonstrated that ATF4 interacts with ACSL4 in HAECs. …”
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93
Biparatopic binding of ISB 1442 to CD38 in trans enables increased cell antibody density and increased avidity
Published 2025-12-01“…The predicted antibody-antigen stoichiometries at different CD38 surface densities were experimentally validated by surface plasmon resonance and cell binding assays. The specific design and structural features of ISB 1442 enable: 1) enhanced trans binding to adjacent CD38 molecules to increase Fc density at the cancer cell surface; 2) prevention of avid cis binding to monomeric CD38 to minimize blockade by soluble shed CD38; and 3) greater binding avidity, with a slower off-rate at high CD38 density, for increased specificity. …”
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94
Compatibility and antimicrobial activity of silver nanoparticles synthesized using Lycopersicon esculentum peels
Published 2024-11-01“…Results of UV–visible spectroscopy recorded surface plasmon resonance at 310 nm for SPR of 2.5. The DLS results showed particles of 186 nm with a polydispersity index of 0.573. …”
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95
Magnesium ions regulate the Warburg effect to promote the differentiation of enteric neural crest cells into neurons
Published 2025-01-01“…D-glucose-13C6 isotope tracing identified key glucose flux changes. Surface plasmon resonance was used to detect the binding affinity of magnesium ions with key glycolysis genes. …”
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96
Exploring Innovative Approaches for the Analysis of Micro- and Nanoplastics: Breakthroughs in (Bio)Sensing Techniques
Published 2025-01-01“…Plasmonic techniques, including surface plasmon resonance (SPR) and surface-enhanced Raman spectroscopy (SERS), provide high sensitivity and specificity through nanostructure-enhanced detection. …”
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97
Tau oligomers impair memory and synaptic plasticity through the cellular prion protein
Published 2025-01-01“…Similarly, TauOs inhibited long-term potentiation in acute hippocampal slices from WT but not Prnp 0/0 mice. Surface plasmon resonance indicated a high-affinity binding between TauOs and PrPC with a K D of 20–50 nM. …”
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98
Metformin blocks BIK1-mediated CPK28 phosphorylation and enhances plant immunity
Published 2025-02-01“…Additionally, surface plasmon resonance (SPR) and microscale thermophoresis (MST) techniques were employed to identify MET targets. …”
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99
Nitidine chloride inhibits the progression of hepatocellular carcinoma by suppressing IGF2BP3 and modulates metabolic pathways in an m6A-dependent manner
Published 2025-02-01“…The target effect of NC on IGF2BP3 was verified through RT-qPCR, WB, molecular docking, molecular dynamics (MD) simulation, surface plasmon resonance (SPR), and CCK8 off-target assays. …”
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100
Eco-friendly synthesis of silver nanoparticles: Enhancing optimization reaction, characterization, and antimicrobial properties with Lantana camara from geothermal area
Published 2025-01-01“…The AgNPs exhibited a spherical shape with a surface plasmon resonance (SPR) peak at a wavelength of 417 nm, and the smallest particle size measured was 44 nm. …”
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