Diffusion models for super-resolution microscopy: a tutorial by Harshith Bachimanchi, Giovanni Volpe

“…In this tutorial, we provide a comprehensive guide to build denoising diffusion probabilistic models from scratch, with a specific focus on transforming low-resolution microscopy images into their corresponding high-resolution versions in the context of super-resolution microscopy. We provide the necessary theoretical background, the essential mathematical derivations, and a detailed Python code implementation using PyTorch. …”
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Super-resolution microscopy as a drug discovery tool by Lauren Toms, Lorna FitzPatrick, Philip Auckland

“…Now, after nearly two decades, super-resolution microscopy has begun to address previously unmet challenges in the study of human disease and is poised to become a pivotal tool in drug discovery.…”
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Super-resolution microscopy: Shedding new light on blood cell imaging by Huan Deng, Yan Ma, Yu-Hui Zhang

“…The examination of subcellular structures and proteins within blood cells at the nanoscale can provide valuable insights into the health status of an individual, accurate diagnosis, and efficient treatment strategies for diseases. Super-resolution microscopy (SRM) has recently emerged as a cutting-edge tool for the study of blood cells, providing numerous advantages over traditional methods for examining subcellular structures and proteins. …”
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Isolation of Viral Biofilms From HTLV-1 Chronically Infected T Cells and Integrity Analysis by Coline Arone, Hélène Dutartre, Delphine Muriaux

“…This method circumvents the need for mechanical, chemical, or enzymatic biofilm detachment, bypasses the use of ultracentrifugation, and enables us to resuspend the biofilms in the appropriate buffer for subsequent analyses such as western blotting or super-resolution microscopy imaging as presented.…”
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Advanced Methods in Fluorescence Microscopy by Luke Fritzky, David Lagunoff

“…The gamut extends from established confocal laser scanning through multiphoton and TIRF to the emerging technologies of super-resolution microscopy that breech the Abbé limit of resolution. …”
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Evidence of Presynaptic Localization and Function of the c-Jun N-Terminal Kinase by Silvia Biggi, Lucia Buccarello, Alessandra Sclip, Pellegrino Lippiello, Noemi Tonna, Cristiano Rumio, Daniele Di Marino, Maria Concetta Miniaci, Tiziana Borsello

“…By using N-SIM Structured Super Resolution Microscopy as well as biochemical approaches, we demonstrated that presynaptic fractions contained significant amount of JNK protein and its activated form. …”
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Aggregation‐based dual‐target probe for dual‐colour super‐resolution monitoring mitophagy and evaluating drugs regulating mitochondria by Xiu‐Zhi Yang, Hongbao Fang, Shumeng Li, Chengyan Chu, Yunhua Zhang, Ying Yang, Weijiang He, Yuncong Chen, Zijian Guo

“…We apply AHOH in super‐resolution microscopy and clearly visualize the dynamics of mitochondria–lysosomes interactions and the oxidative stress states upon different stimuli. …”
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Lipidic folding pathway of α-Synuclein via a toxic oligomer by Vrinda Sant, Dirk Matthes, Hisham Mazal, Leif Antonschmidt, Franz Wieser, Kumar T. Movellan, Kai Xue, Evgeny Nimerovsky, Marianna Stampolaki, Magdeline Nathan, Dietmar Riedel, Stefan Becker, Vahid Sandoghdar, Bert L. de Groot, Christian Griesinger, Loren B. Andreas

“…We report an atomic-resolution structural characterization of a toxic pre-fibrillar aggregation intermediate (I1) on pathway to the formation of lipidic fibrils, which incorporate lipid molecules on protofilament surfaces during fibril growth on membranes. Super-resolution microscopy reveals a tetrameric state, providing insights into the early oligomeric assembly. …”
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Fluorescence engineering in metamaterial-assisted super-resolution localization microscope by Choi Kyu Ri, Li Shilong, Ozerov Igor, Bedu Frédéric, Park Dong Hee, Joo Bin Chan, Wu Jeong Weon, Nic Chormaic Síle, Lee Yeon Ui

“…In contrast to conventional localization microscopes, which rely on particular fluorescent probes in specific conditions, metamaterial-assisted super-resolution microscopies can be implemented with any fluorescent dye under general conditions. …”
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