Nestin Forms a Flexible Cytoskeleton by Means of a Huge Tail Domain That Is Reversibly Stretched and Contracted by Weak Forces by Ayana Yamagishi, Rina Tokuoka, Kazuki Imai, Mei Mizusawa, Moe Susaki, Koki Uchida, Saku T. Kijima, Akira Nagasaki, Daijiro Takeshita, Chiaki Yoshikawa, Taro Q. P. Uyeda, Chikashi Nakamura

“…Nestin is a type VI intermediate filament protein and a well-known neural stem cell marker. It is also expressed in high-grade cancer cells, forming copolymerized filaments with vimentin. …”
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Role of Leucine-Rich Repeat–Containing G-Protein-Coupled Receptors 4–6 (LGR4–6) in the Ovary and Other Female Reproductive Organs: A Literature Review by Yu-Hsun Chang, Kun-Chi Wu, Kai-Hung Wang, Dah-Ching Ding

“…LGR6 is a marker for fallopian tube stem cells and is involved in stem cell maintenance and differentiation. …”
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Standard: Human gastric organoids by Fan Hong, Ronghui Tan, Ting Wang, Nanshan Zhong, Hongling Zhao, Rui-hua Xu, Lin Shen, Yingbin Liu, Xuebiao Yao, Dongxi Xiang, Lijian Hui, Jianping Xiong, Dong Gao, Bing Zhao, Zhifeng Miao, Jie Hao, Yong Li, Shijun Hu, Boqiang Fu, Guoqiang Hua, Lei Wang, Zhao-Lei Zeng, Chong Chen, Jianmin Wu, Changlin Wang, Chunnian Wang, Xianbao Zhan, Chen Song, Chunping Yu, Yingying Yang, Gengming Niu, Yalong Wang, Tongbiao Zhao, Ye-Guang Chen

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Advances in the Development and Application of Human Organoids: Techniques, Applications, and Future Perspectives by Zhangcheng Zhu, Yiwen Cheng, Xia Liu, Wenwen Ding, Jiaming Liu, Zongxin Ling, Lingbin Wu

“…Organoids are three-dimensional (3D) cell cultures derived from human pluripotent stem cells or adult stem cells that recapitulate the cellular heterogeneity, structure, and function of human organs. …”
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Generation of live mice from haploid ESCs with germline-DMR deletions or switch by Yongjian Ma, Meng Yan, Zhenfei Xie, Hongling Zhang, Zhoujie Li, Yuanyuan Li, Suming Yang, Meiling Zhang, Wen Li, Jinsong Li

“…Here, we establish a stepwise system to reconstruct a development-related imprinting network, in which diploid embryonic stem cells (ESCs) are derived by fusing between parthenogenetic (PG)- and androgenetic (AG)-haploid embryonic stem cells (haESCs) with different DMR deletions (termed Ha-Ha-fusion system), followed by tetraploid complementation to produce all-haESC fetuses. …”
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USP39 phase separates into the nucleolus and drives lung adenocarcinoma progression by promoting GLI1 expression by Shaoxuan Cheng, Zhiyuan Qiu, Ziyi Zhang, Yuxuan Li, Yue Zhu, Yuxin Zhou, Yinghui Yang, Yaowen Zhang, Dian Yang, Yingqiu Zhang, Han Liu, Zhaoxia Dai, Shu-Lan Sun, Shuyan Liu

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MiRNAs as major players in brain health and disease: current knowledge and future perspectives by Sarika V. Kapplingattu, Sujata Bhattacharya, Yogita K. Adlakha

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Regulatory roles of RNA binding proteins in the Hippo pathway by Shuchang Peng, Chenglin Li, Yanwen He, Lei Xue, Xiaowei Guo

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The role of circadian rhythm regulator PERs in oxidative stress, immunity, and cancer development by Baimei Zhao, Eugenie Nepovimova, Qinghua Wu

“…PER2/3, as antagonists in cancer stem cell biology, play important roles in differentiating cancer stem cells and in maintaining their stemness. …”
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Comparative analysis of pediatric SHH medulloblastoma DAOY spheres and adherent monolayers: implications for medulloblastoma research by Jana Klementová, Šárka Jarošová, Irina Danilová, Markéta Farníková, Josef Novotný, Marie Davídková, Martina Zíková

“…Our findings demonstrate that DAOY spheres express markers for both neural stem cells, such as CD133 (PROM1), and differentiated neurons, exemplified by MAP2. …”
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Intestinal organoids: The path towards clinical application by Hady Yuki Sugihara, Ryuichi Okamoto, Tomohiro Mizutani

“…In this review, we outline the current state of translational research involving adult stem cell and pluripotent stem cell derived intestinal organoids, highlighting the advances and limitations in disease modeling, drug-screening, personalized medicine, and stem cell therapy. …”
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Rapid iPSC-derived neuromuscular junction model uncovers motor neuron dominance in amyotrophic lateral sclerosis cytopathy by Hsiao-Chien Ting, Yun-Ting Guo, Hong-Lin Su, Yu-Shuan Chen, Shinn-Zong Lin, Horng-Jyh Harn, Chia-Yu Chang

“…Although human induced pluripotent stem cell (iPSC)-derived models have advanced NMJ research, their application is constrained by challenges such as limited differentiation efficiency, lengthy generation times, and cryopreservation difficulties. …”
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Estrogen-dependent activation of TRX2 reverses oxidative stress and metabolic dysfunction associated with steatotic disease by Alfredo Smiriglia, Nicla Lorito, Marina Bacci, Angela Subbiani, Francesca Bonechi, Giuseppina Comito, Marta Anna Kowalik, Andrea Perra, Andrea Morandi

“…In this study, we utilized preclinical in vitro models—immortalized cell lines and hepatocyte-like cells derived from human embryonic stem cells—exposed to clinically relevant steatotic-inducing agents. …”
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The AhR pathway regulation in phthalates-induced cancer promotion, progression and metastasis: a scoping review by Mostafa Akbariani, Mahmoud Omidi, Zohreh Shahabi, Hamed Haghi-Aminjan, Amir Shadboorestan

“…Specifically, DEHP activated AhR, leading to elevated expression of EMT markers, increased cancer stem cell populations, and enhanced drug metabolism and resistance. …”
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Inhibition of TFF3 synergizes with c-MET inhibitors to decrease the CSC-like phenotype and metastatic burden in ER+HER2+ mammary carcinoma by Chuyu He, Xuejuan Wang, Yi-Shiou Chiou, Basappa Basappa, Tao Zhu, Vijay Pandey, Peter E. Lobie

“…Elevated expression of TFF3 promoted the oncogenicity of ER+HER2+ MC cells, including enhanced cell proliferation, survival, anchorage-independent growth, 3D growth, cancer stem cell-like (CSC-like) phenotype, migration, invasion, and xenograft growth. …”
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Human-based complex in vitro models: their promise and potential for rare disease therapeutics by Surat Parvatam, Francesca Pistollato, Lindsay J. Marshall, Fabia Furtmann, Devashree Jahagirdar, Mohua Chakraborty Choudhury, Sujata Mohanty, Harshita Mittal, Saveetha Meganathan, Rakesh Mishra

“…Complex in vitro models (CIVMs), including those using induced pluripotent stem cells (iPSCs), organoids, and organs-on-chips are emerging as powerful human-based pre-clinical systems with the capacity to provide efficacy data enabling drugs to move into clinical trials. …”
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CDK14 regulates the development and repair of lung by Jian-wei Chen, Yu-xiang Wang, Rong-rong Gao, Lan-yue Ma, Jing Zhong, Jia-xin Yang, Zhao-hua Deng, Yu-yan Li, Xiao-ling Li, Ya-hai Shu, Wen-jing Guo, Zi-yuan Zhou, Xiao Yu Tian, Jinjin Ma, Yang Liu, Qi Chen

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Synthetic embryology of the human heart by Maria Belen Paredes-Espinosa, Janet L. Paluh

“…The evolution of stem cell-based heart models from cells and tissues to organoids and assembloids and recently synthetic embryology gastruloids, is poised to revolutionize our understanding of cardiac development, congenital to adult diseases, and patient customized therapies. …”
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Exocyst complex component 1 (Exoc1) loss in dormant oocyte disrupts c-KIT and growth differentiation factor (GDF9) subcellular localization and causes female infertility in mice by Chi Lieu Kim Nguyen, Yumeno Kuba, Hoai Thu Le, Hossam Hassan Shawki, Natsuki Mikami, Madoka Aoki, Nanako Yasuhara, Hayate Suzuki, Saori Mizuno-Iijima, Shinya Ayabe, Yuki Osawa, Tomoyuki Fujiyama, Tra Thi Huong Dinh, Miyuki Ishida, Yoko Daitoku, Yoko Tanimoto, Kazuya Murata, Woojin Kang, Masatsugu Ema, Yuji Hirao, Atsuo Ogura, Satoru Takahashi, Fumihiro Sugiyama, Seiya Mizuno

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Vitronectin regulates focal adhesion turnover and migration of human placenta-derived MSCs under nutrient stress by Srishti Dutta Gupta, Nitish Pal, Malancha Ta

“…At sites of tissue damage and wound healing, the mesenchymal stem cells (MSCs) are often challenged by nutrient availability due to blood supply disruption. …”
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