High-intensity focused ultrasound: an innovative approach for micro-manipulation of demineralized dentine by Sheetal Maria Rajan, Barsha Shrestha, Amr Fawzy

“…Confocal microscopy of Rhodamine-B stained ACSD (n = 5/group) quantified the depth of dentine microscale removal post-HIFU exposure in a time-dependent manner. …”
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GOLPH3 inhibits glioma cell apoptosis through the JNK signaling pathway by Shao Xie, Shao Xie, Jiahai Ding, Zhaohao Wang, Hengliang Shi, Zheng-Quan Yu

“…Apoptosis was assessed using flow cytometry, immunofluorescence staining, TUNEL assays, and Western blotting. The activation of the JNK signaling pathway was evaluated by analyzing the phosphorylation levels of JNK and c-Jun through Western blotting.ResultsDownregulation of GOLPH3 in U87 glioma cells significantly enhanced apoptosis, as evidenced by increased levels of cleaved caspase-3 and higher apoptosis rates. …”
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New taphonomic approach applied to the Late Pleistocene bone remains from Pikimachay Cave, Ayacucho Basin, Peru: possible implications for the debate on human colonisation of weste... by Hugo G Nami, Karina V Chichkoyan, Juan Yataco Capcha, José L Lanata

“…For this purpose, we examined the surface modifications indicating fresh or post-depositional fractures, the different kinds of marks, weathering, and the presence of manganese staining. We concluded that several bones (n=8) from layer h showed various human-made modifications. …”
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Evaluation of the Anti-Cancer Effect of TRAF2 and NCK In teracting Protein Kinase (TNIK) Inhibition in Breast Cancer Cells by Kaan Furkan Hamarat, Gamze Güney Eskiler, Selin Zeynep Özçelik, Süleyman Kaleli

“…Acridine Orange/Propidium Iodide (AO/PI) staining was performed to evaluate the effect of NCB-0846 on cellular morphology in the MCF-7 cell line. …”
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Pathological characterization of lung fibrosis in Sprague–Dawley rats treated with fluoro-edenite fibres by intrapleural injection by Eva Tibaldi, Federica Gnudi, Daniele Mandrioli, Caterina Bruno, Amerigo Zona, Lucia Fazzo, Pietro Comba

“…Immunohistochemical analysis of protein involved in fibrotic responses and histochemical staining for FE-fibres identification were performed. …”
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Nuclear DNA Content and Chromatin Pattern of Rat Rhabdomyosarcoma Cell Sublines with Different Metastatic Potentials by Jean Dufer, Marie-France Poupon, Sonia Yatouji

“…The aim of this work was to characterize nuclear changes observed in metastatic cell clones issued in vitro from the same parental cell population The nuclear phenotypes of 6 cell sublines isolated from a rat rhabdomyosarcoma cell line and differing in their metastatic ability were evaluated by image cytometry on Feulgen‐stained preparations. Densitometric [5], geometric [3] and textural [9] features were computed from each nuclear image. …”
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Reduced Fascicle Area Demonstrated in Ilioinguinal Nerves Resected from Primary Inguinal Herniorrhaphy Patients as Evidence of Compression Neuropathy by Robert Wright, Donald E. Born, Troy Sanders, Jordan Landes, Troy Salisbury, Anjali S. Kumar, Makena Horne

“…Three samples of the resected ilioinguinal nerve (proximal, canal, and distal) were evaluated using Masson’s trichrome stain to measure fascicle and total nerve cross-sectional area and detect changes in collagen. …”
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Trichomonas vaginalis and Associated Factors among Pregnant Women Attending Antenatal Care at Bule Hora University Teaching Hospital, Oromia Region, Southern Ethiopia by Oliyad Husen, Alqeer Aliyo, Kalicha Boru, Tibeso Gemechu, Wako Dedecha, Girma Ashenafi

“…The two vaginal swabs were collected by brushing the vagina with a sterile cotton swab and tested by using direct wet mount and the Giemsa staining. The data were analyzed using SPSS version 26 for logistic regression analysis. …”
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Comparative efficacy of 0.1% and 0.15% Sodium Hyaluronate on lipid layer and meibomian glands following cataract surgery: A randomized prospective study. by Seung Ahn Yang, Mu Ryang Jeong, Cheon Ho Park, Ki Bum Cheon, Jun Ho Chang, Ji Eun Lee

“…Subjective and objective assessments were performed at preoperative and postoperative visits, including ocular surface disease index score, tear break up time, corneal staining score, Schirmer's I test score, lipid layer thickness), meiboscore, and biochemical analysis of the eye drops.…”
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Abdominal Massage Improves the Symptoms of Irritable Bowel Syndrome by Regulating Mast Cells via the Trypase-PAR2-PKCε Pathway in Rats by Huanan Li, Wei Zhang, Fei Ma, Xiaofan Zhang, Yuyan Wang, Jingui Wang

“…On day 14, the rats were euthanized and the tissues were analyzed by transmission electron microscopy (TEM), immunohistochemistry or immunofluorescence staining, and laser confocal focus to visualize the micromorphology of the intestinal mucosa. …”
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Inhibition of miR-652-3p Regulates Lipid Metabolism and Inflammatory Cytokine Secretion of Macrophages to Alleviate Atherosclerosis by Improving TP53 Expression by Haiyun Liu, Changpeng Zuo, Lijuan Cao, Naiquan Yang, Tingbo Jiang

“…After ox-LDL treatment and miR-652-3p mimic, si-TP53 and their controls transfection, ELISA, and Q-PCR assays were used to detect IL-1ß, IL-6, and TNF-α levels. oil red O staining was processed to verify cholesterol accumulation. …”
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Proteomic characterization of molecular mechanisms of paraquat-induced lung injury in a mouse model by Yu Qing Zhou, Jin Jin Peng, Li Ping Shan, Wei Liu

“…Lung pathological changes were observed with conventional staining techniques. Lung tissue components were assessed with tandem mass spectrometry tag technology, and differentially expressed proteins (DEPs) were bioinformatically analyzed and investigated with parallel reaction monitoring. …”
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Differential expression of DNA fragmentation factor 45 in leukoplakia and oral squamous cell carcinoma and its correlation with TNF-α levels by Sabitha Balakrishnan, Ramya Ramadoss, Rajashree Padmanaban, Rajkumar Krishnan

“…Materials and methods: Surgically excised specimens from patients with primary OSCC (n = 20) leukoplakia (n = 30) and normal oral mucosa (n = 25) were immuno stained for DFF-45. The relationship of DFF-45expression with clinical parameters and histological grading was also evaluated. …”
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TGF-β1 inhibitor enhances the therapeutic effect of microwave ablation on hepatocellular carcinoma by Shufang Xiang, Juan Li, Mei Zhang

“…Histopathological changes were examined by hematoxylin and eosin staining. The mechanisms associated with the function of MWA combined with TGF-β1 inhibitor in HCC development were explored by western blotting.Results Combination of MWA and SB-525334 decreased the survival rate and promoted the apoptosis of HCC cells compared with MWA alone. …”
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Acute Genetic Damage Induced by Ethanol and Corticosterone Seems to Modulate Hippocampal Astrocyte Signaling by Ana Laura Reyes-Ábalos, Magdalena Álvarez-Zabaleta, Silvia Olivera-Bravo, María Vittoria Di Tomaso

“…Genetic damage and initial DDR were assessed by immunolabeling against the phosphorylated histone variant H2AX (γH2AX), DDR-dependent apoptosis by BAX immunoreactivity, and astrocyte activation by the glial acidic fibrillary protein (GFAP) and phalloidin staining. Surface-attached EVs and NTs were examined via scanning electron microscopy, and labeled proteins were analyzed via confocal microscopy. …”
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In vitro antitumor effects of methanolic extracts of three Ganoderema mushrooms by Elshahat A. Toson, Amira A. El-Fallal, Marwa A. Oransa, Hoda M. El-Gharabawy

“…In this regard, Sulfo-rhodamine B staining and flow cytometry were used. Hepatocellular carcinoma (HepG2) and breast ductal carcinoma (T-47D) cell lines were used as cancer models, while mouse normal liver (BNL) and oral epithelial cell (OEC) lines were used as respective controls. …”
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Gastrointestinal Parasites With Their Risk Factors in Tharu Indigenous People in Southern Nepal: A Cross‐Sectional Study by Pinki Kumari Chaudhary, Tirth Raj Ghimire

“…The samples were analyzed by direct wet mount and acid‐fast staining techniques and examined at 40×, 100×, 400×, and 1000× magnifications. …”
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Surgical treatment of epirethinal fibrosis: a current state of the problem by S.A. Kochergin, D.G. Alipov

“…The article describes the features for visualization of ERM during surgery and a comparison of the results of operations involving ERM staining with ILM-Blue and MembraneBlue-Dual. The role of OCT in the diagnostics and treatment of ERF is shown. …”
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Effect of adipose mesenchymal stem cell-derived exosomes on allergic rhinitis in mice by Xiaojie Xu, Chang Li, Qin Qin, Feiyan Han, Ying Wang

“…Mice were sacrificed, nasal mucosa was collected for HE staining and qRT-PCR. Results: After injection of AMSC-exos, compared with the AR + PBS group, symptoms were significantly improved, inflammatory factor levels were significantly decreased, disturbed and thickened mucosal layer was relieved, and the numbers of goblet cells and eosinophils were reduced in the AR + AMSC-exos group. …”
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Primary Mechanism Study of Panax notoginseng Flower (Herb) on Myocardial Infarction in Rats by Xin Zhou, Zhi-Cheng Li, Pei-Pei Chen, Rui-Fang Xie

“…HE (hematoxylin and eosin) staining, immunofluorescent assay, TUNEL assay, quantitative real-time PCR, and western blot analysis were, respectively, performed to observe morphology, count mean minimal vessels, investigate apoptotic cells, and record gene (HIF-1, VEGFA, and KDR) and protein (Bcl-2 and Bax) expressions. …”
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