Nuclear DNA content variation within four species of Asian catfish of family Pangasidae and their two interspecific hybrids by flow cytometry by Imron, Evi Tahapari, Jadmiko Darmawan, Muhammad Luthfi Abdurachman

“…Red blood cells (RBC) were taken from the respective species/groups and NDC measurement was performed in an Attune acoustic flowcytometer (ABI) using DAPI staining and chicken, Gallus domesticus, RBC was used as size reference. …”
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Tyrosine phosphatase SHP2 accelerated ovarian cancer via modulating integrin/ E-Cadherin/ ZEB1 induced EMT by Xiaofei Li, Haibo Zhang, Jianan Dong, Juan Wang

“…Detection of expression of p-SHP2, ZEB1, E-Cadherin, α3-integrin, p-Src, p-SMAD2, Snail, Slug and SKOV3 cells of migration and invasion abilities were detected using Western blot method and cell scratch assay and Transwell assay; Progression of ovarian cancer was detected using subcutaneous tumor transplantation assay in nude mice and HE staining method and immunocyto. The bioinformatics analysis results suggested that SHP2 is highly specifically expressed and E-Cadherin, which is low specifically expressed in ovarian cancer tissues, while EGF, p-SHP2 or ZEB1 are highly expressed and E-Cadherin is low expressed in ovarian cancer tissues; Overexpression of E-Cadherin could reduce the expressions of p-SHP2, ZEB1, α3-integrin, p-Src, p-SMAD2, Snail and Slug might has roles in alleviating the ovarian cancer development and decreasing the levels of p-SHP2 and ZEB1 in tumor samples. …”
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YAP Overexpression in Breast Cancer Cells Promotes Angiogenesis through Activating YAP Signaling in Vascular Endothelial Cells by Yu Yan, Qiang Song, Li Yao, Liang Zhao, Hui Cai

“…Expression of proteins and phosphorylating proteins involved in YAP, G13-RhoA, and PI3K/Akt signaling pathways was evaluated using the Western blotting, immunofluorescence staining, and immunohistochemistry analysis. In addition, the effects of YAP on breast cancer angiogenesis were evaluated in vivo by tumor xenograft mice. …”
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Novel silver nanoparticle-based biomaterials for combating Klebsiella pneumoniae biofilms by Eslam Elashkar, Rihaf Alfaraj, Ola M. El-Borady, Mahmoud M. Amer, Abdelazeem M. Algammal, Azza S. El-Demerdash

“…The antibiofilm activity of AgNPs@chitosan-NaF was evaluated using crystal violet staining and scanning electron microscopy. The underlying mechanisms of action were investigated through gene expression analysis.ResultsThe majority of K. pneumoniae isolates exhibited high levels of multidrug resistance. …”
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Emodin promotes the recovery of rheumatoid arthritis by regulating the crosstalk between macrophage subsets and synovial fibroblast subsets by Lianying Cheng, Xiaofeng Rong

“…A collagen‐induced arthritis mouse model was constructed to investigate the therapeutic potential of emodin in RA by flow cytometry, micro‐CT and staining. Results Unique subsets of FLSs and STMs, namely, FAPα+THY1− FLSs, FAPα+THY1+ FLSs, and MerTKposTREM2high STMs, were identified in synovial tissues from RA patients. …”
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The Expression of Thymic AQP7 and Perilipin 1 (PLIN1) in Rats Fed a High-Fructose Diet is Modified by Voluntary Physical Activity by Jülide Tozkır, Nihayet Fırat, Ebru Göncü, Onur Ersoy, Pınar Tayfur, Orkide Palabıyık

“…Histological evaluation of the thymus was performed by hematoxylin-eosin staining. The expression levels of AQP7 and PLIN1 were determined real-time reverse transcription polymerase chain reaction using appropriate primers. …”
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Histomorphometrical Study on Regional Variation in Distribution of Sweat Glands in Buffalo Skin by Debajit Debbarma, Varinder Uppal, Neelam Bansal, Anuradha Gupta

“…The paraffin sections of 5-6 μm were cut with rotary microtome and stained with hematoxylin and eosin. The sweat glands in buffaloes were of saccular and simple coiled tubular type. …”
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Upregulated Solute Carrier SLC39A1 Promotes Gastric Cancer Proliferation and Indicates Unfavorable Prognosis by Dan Yu, Yong Chen, Ming Luo, Yanjin Peng, Shengen Yi

“…A retrospective gastric adenocarcinoma cohort (n = 154) was collected from our hospital to test their tissue expression of SLC39A1 through immunohistochemical staining method. After SLC39A1 overexpression or knockdown, proliferation and invasion assays were conducted for proliferation and invasion estimation, respectively. …”
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A Novel “Slit Side View” Method to Evaluate Fluid Dynamics during Phacoemulsification by Hisaharu Suzuki, Tsutomu Igarashi, Toshihiko Shiwa, Hiroshi Takahashi

“…In this experimental study, extracted porcine eyes were injected with OVDs stained with fluorescein through a side port of the eye and then fixed on a slit lamp microscope. …”
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Impact of Notch3 Activation on Aortic Aneurysm Development in Marfan Syndrome by Kathryn Jespersen, Chenxin Li, Rishi Batra, Christopher A. Stephenson, Paul Harding, Kylie Sestak, Ryan T. Foley, Harrison Greene, Trevor Meisinger, Jason R. Cook, B. Timothy Baxter, Wanfen Xiong

“…Aortic aneurysms and rupture were evaluated with connective tissue staining, ultrasound, and life table analysis. Results. …”
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Differentially Expressed MicroRNAs in the Development of Early Diabetic Retinopathy by Qiaoyun Gong, Jia’nan Xie, Yang Liu, Ying Li, Guanfang Su

“…HREC/RPE viability decreased after 24 h incubation and diminished further over 6 days, and Hoechst staining revealed hyperglycemia-induced HREC/RPE apoptosis. …”
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Contribution of domestic animals’ feces to the occurrence of diarrhoea among children aged 6–48 months in Sidama region, Ethiopia: a laboratory-based matched case-control study by Argaw Ambelu, Gorfu Geremew Gunsa, Alemayehu Haddis

“…The presence of diarrhoea-causing pathogens (Campylobacteria, Escherichia coli, non-typhoidal salmonella, Shigella and Cryptosporidium) was detected using culture media, biochemical tests, gram stain, catalase and oxidase tests. The diarrhoea risk factors were identified using conditional logistic regressions and the random forest method using R.4.3.2.Results Of the faecal specimens diagnosed, 250 (64.1%) tested positive for one or more pathogens. …”
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Long Noncoding RNA SNHG4 Attenuates the Injury of Myocardial Infarction via Regulating miR-148b-3p/DUSP1 Axis by Sheng Wang, Zhaoyun Cheng, Xianjie Chen, Guoqing Lu, Xiliang Zhu, Gaojun Xu

“…The myocardial injury in MI rats was evaluated by echocardiography, TTC/HE/MASSON/TUNEL staining, and immunohistochemistry (Ki67). DLR assay was performed to confirm the target relationships. …”
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A Study Comparing Immunohistochemistry, Fluorescence in situ hybridization, GeneXpert® Breast Cancer STRAT4 Assay for Determining Hormone Receptor, Human Epidermal Growth Factor Re... by Rajaa El Aje, Abdellah Naya, Ayoub Khoaja, Younes Zaid, Mounia Oudghiri, Jodi Weidler, Mehdi Karkouri

“…The Xpert® Breast Cancer STRAT4, an RT-qPCR based system, can be used to classify invasive breast carcinomas based on the assessment of these four biomarkers; Methods: We evaluated ESR1, PGR, ERBB2, and MKi67 mRNA expression by Xpert Breast Cancer STRAT4 and ER, PR, HER2 and Ki67 by IHC (FISH for HER2 IHC 2+) in 200 formalin-fixed paraffin-embedded (FFPE) tissue blocks with invasive breast cancer, collected from the Pathology Department of Casablanca Ibn Rochd University Hospital; Results: Concordance between Xpert ® Breast Cancer STRAT4 and IHC was 93.5% for ER, 83.51% for PR, 95% for HER2 (92% for IHC+FISH), and 81.20% for Ki67 (excluding intermediate IHC Staining 10 ≤ %IHC <20). The simple Kappa coefficient was, for ER, 0.830 (P < 0.0001), 0.565 (P < 0.0001) for PR, 0.838 (P < 0.0001) for HER2-IHC, 0.771 (P < 0.0001) for HER2 IHC+FISH and, for, Ki67, 0.458 (P < 0.0001); Conclusions: We demonstrated globally a high concordance between centrally assessed IHC, IHC+FISH and mRNA measurements of ER/ESR1 and HER2/ERBB2, and a moderate agreement between PR/PGR and Ki67/MKi67. …”
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Gastrospheres as a Model of Gastric Cancer Stem Cells Skew Th17/Treg Balance toward Antitumor Th17 Cells by Alaleh Rezalotfi, Ghasem Solgi, Marzieh Ebrahimi

“…The proliferation was evaluated using CFSE staining; the percentages of CD4+CD25+FoxP3+ Treg and CD4+IL-17+ Th17 cells and IFN-γ+cells and the production of IL-17, TGF-β, and IL-10 were assessed by flow cytometry and ELISA, respectively. …”
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Expression of cytokeratin 19 in prostatic adenocarcinoma: a systematic review by Sina Shahshenas, Seyyed Mohammad Hosseini, Hossein Yarmohammadi, Masood Soltanipur, Mohammadreza Jalali Nadoushan

“…Eleven studies used reverse transcription polymerase chain reaction (RT-PCR) to investigate CK-19 expression, four used immunohistochemistry (IHC) staining, three used both one-step nucleic acid amplification (OSNA) and hematoxylin and eosin (H&E) methods, and three used the electrochemiluminescence (ECL) technique. …”
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Influence of Adipose Tissue-Derived Stem Cells on the Burn Wound Healing Process by Claudio Luciano Franck, Alexandra Cristina Senegaglia, Lidiane Maria Boldrini Leite, Sérgio Adriane Bezerra de Moura, Nathália Forti Francisco, Jurandir Marcondes Ribas Filho

“…The progress was evaluated by immunohistochemical methods and H&E, Masson’s trichrome, Picrosirius red, and Lyve-1 immunofluorescence staining. Despite the quantitative similarity of blood vessels and the inflammatory infiltrate observed by H&E, there were statistically significant differences between the groups on the fourteenth day of evolution. …”
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Multiple Primary Angiosarcomas of the Colon by Sonja Radić, Mario Zovak, Anita Galović Marić, Stjepan Baturina, Monica Stephany Kirigin, Božo Krušlin

“…A definitive pathological diagnosis relies on immunohistochemical staining for endothelial markers. Misdiagnosis as poorly differentiated adenocarcinoma or GIST is possible in limited tissue biopsies.…”
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Follow up through Endoscopical – Histological Studies and Helicobacter Pylori Infections in Patients Suffering from Gastric Ulcers by Marcos Félix Osorio Pagola, Antonio Moré Pérez, Libán Álvarez Cáceres, Juan Luis de Pasos Carrazana, Anagalys Ortega Alvelay

“…Endoscopy and biopsy of the gastric mucosa was done for the histological study of the gastric ulcers and for the diagnosis of infection due to Helicobacter pylori through hematoxiline-eosine and giemsa stains respectively. <strong><br />Results</strong>: 89 patients (92,7 %) healed their ulcers in the first three months of follow up and 5 patients underwent a histological diagnosis of malignant ulcers (5,2 %). …”
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The Protective Effect of Naringin against Bleomycin-Induced Pulmonary Fibrosis in Wistar Rats by Nergiz H. Turgut, Haki Kara, Sahende Elagoz, Koksal Deveci, Huseyin Gungor, Emre Arslanbas

“…Tissue sections were stained with hematoxylin-eosin, Masson’s trichrome, and 0.1% toluidine blue. …”
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