Dendrobium chrysotoxum Lindl. Alleviates Diabetic Retinopathy by Preventing Retinal Inflammation and Tight Junction Protein Decrease by Zengyang Yu, Chenyuan Gong, Bin Lu, Li Yang, Yuchen Sheng, Lili Ji, Zhengtao Wang

“…Western blot analysis and retinal immunofluorescence staining results further confirmed that DC reversed the decreased expression of occludin and claudin-1 proteins in diabetic rats. …”
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The Protective Effect of Low-Dose Ethanol on Myocardial Fibrosis through Downregulating the JNK Signaling Pathway in Diabetic Rats by Ying Yu, Xian-Jie Jia, Wei-ping Zhang, Ting-ting Fang, Jie Hu, Shan-Feng Ma, Qin Gao

“…Morphology of collagen in myocardial tissue was observed with Masson’s trichrome staining method and collagen volume fraction (CVF) was analysed. …”
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Andrographolide Protects against HG-Induced Inflammation, Apoptosis, Migration, and Impairment of Angiogenesis via PI3K/AKT-eNOS Signalling in HUVECs by Ming-Xia Duan, Heng Zhou, Qing-Qing Wu, Chen Liu, Yang Xiao, Wei Deng, Qi-Zhu Tang

“…Western blot analysis, real-time PCR, immunofluorescence staining, the scratch test, and the tube formation assay were performed to assess the effects of Andr. …”
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Excretory Function of Intestinal Tract Enhanced in Kidney Impaired Rats Caused by Adenine by Yu Yun, Tao Gao, Yue Li, Zhiyi Gao, Jinlian Duan, Hua Yin, Weigang Duan

“…Kidneys were harvested and histologically examined with hematoxylin-eosin staining kits. Uric acid, urea (BUN), and creatinine in serum were determined with assay kits, and BUN and creatinine in every intestinal segment were also determined. …”
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Automatic deep learning-based colorectal adenoma detection system and its similarities with pathologists by Xin Chen, Wei Xu, Yong Huang, Wei Jin, Gang Xu, Jing Yuan, Zhigang Song, Chunkai Yu, Shuangmei Zou, Wenmiao Wang, Xiaohui Ding, Jinhong Liu, Liwei Shao, Xiangnan Gou, Zhanbo Wang, Huang Chen, Cancheng Liu, Zhuo Sun, Calvin Ku, Yongqiang Zhang, Xianghui Dong, Shuhao Wang, Ning Lv, Huaiyin Shi

“…Meanwhile, it obtains high accuracy on slides collected from different hospitals with significant staining configuration variations.…”
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KAE ameliorates LPS-mediated acute lung injury by inhibiting PANoptosis through the intracellular DNA-cGAS-STING axis by Yonghu Chen, Xilin Wu, Zhe Jiang, Xuezheng Li

“…Lung damage and anti-inflammatory/antioxidant effects were assessed by H&E staining, lung edema index, and SOD, MDA, and ELISA assays. …”
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Cystitis glandularis due to Escherichia coli infection in a diabetic Miniature Schnauzer: a case report by J. P. Rey Amunategui, D. Echeverria, A. Duchene

“…It was decided to perform a surgical cystotomy, which revealed that the surface of the urothelium showed dark hemorrhagic staining and multiple nodular structures, which were relatively hard to palpate. …”
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Expression of Genes Associated with Efflux Pump and Porins in Acinetobacter baumannii Isolates Recovered from Different Clinical Specimens by Alya Amer Rahi, Huda Hadi Al-Hasnawy

“…Bacteria were cultured and identified by Gram stain and biochemical tests and confirmed to be A. baumannii (8.33%) using the Vitek-2 system. …”
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Tacrolimus Protects Podocytes from Apoptosis via Downregulation of TRPC6 in Diabetic Nephropathy by Ruixia Ma, Ying Wang, Yan Xu, Rui Wang, Xianghua Wang, Ning Yu, Minghui Li, Yan Zhou

“…Nephrin and TRPC6 protein expression and podocyte apoptotic rates in vivo and in vitro were measured using immunohistochemical staining, TUNEL assays, and flow cytometry, respectively. …”
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Renal Tissue-Derived Exosomal miRNA-34a in Diabetic Nephropathy Induces Renal Tubular Cell Fibrosis by Promoting the Polarization of M1 Macrophages by Shuai Zheng, Yi Zeng, Liqing Chu, Taiyang Gong, Sihong Li, Min Yang

“…The DN model was established, and the success of the model establishment was confirmed by detecting general indicators, HE staining, and immunohistochemistry. Electron microscopy and NanoSight Tracking Analysis (NTA) were used to see the morphology and size of exosomes. …”
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Combination drug therapy prevents CIAKI by suppressing ER stress-induced apoptosis by Xuan Wang, Shan Han, Lili Zhao, Hongliang Cong

“…Among the five groups, Group PCC presented the significantly highest creatinine increase rate and protein and nucleic acid expression levels, with the most severe cell injury and apoptosis observed via HE and TUNEL staining. Compared with those in the atorvastatin group, the rate of increase in creatinine and protein expression in the combined treatment groups were decreased to some extent, and the histological morphology was also improved. …”
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Nanoemulsion and Encapsulation Strategy of Hydrophobic Oregano Essential Oil Increased Human Prostate Cancer Cell Death via Apoptosis by Attenuating Lipid Metabolism by Haribalan Perumalsamy, Rajeshkumar Shanmugam, Jun-Ran Kim, Gokulanathan Anandapadmanaban, Md. Amdadul Huq, Kamal Dua, Dinesh Kumar Chellappan, Tae Hyun Yoon, Sri Renukadevi Balusamy

“…The lipid biogenesis mediated, molecular target pathway analyses were performed using fluorescence cellular staining techniques, real-time RT-PCR, or western blotting analysis. …”
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Dulaglutide Protects Mice against Diabetic Sarcopenia-Mediated Muscle Injury by Inhibiting Inflammation and Regulating the Differentiation of Myoblasts by Fengyi Deng, Wenyan Wu, Xingyu Fan, Xing Zhong, Nuojin Wang, Yue Wang, Tianrong Pan, Yijun Du

“…Mouse muscle tissues were then pathologically evaluated and stained with F4/80 or MPO to detect macrophages and neutrophils, respectively. …”
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Ischemic Postconditioning Mitigates Retinopathy in Tree Shrews with Diabetic Cerebral Ischemia by Ling Zhao, Qiwei Liao, Yueting Zhang, Shufen Tan, Shuqing Li, Tingyu Ke

“…Tree shrews were divided into the normal control group, sham operation group, diabetes mellitus group, diabetes mellitus+cerebral ischemia group, and diabetes mellitus+cerebral ischemia+PC group (in which the tree shrews with diabetic cerebral ischemia were treated with ischemic PC). H&E staining was used to examine the pathological changes in the retina, and immunohistochemistry was performed to determine the retinal expression of VEGF (vascular endothelial growth factor). …”
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Absence of Tuberculosis-Causing Mycobacteria from Slaughtered Livestock Tissues and Environmental Samples, Gauteng Province, South Africa by Vuyokazi Mareledwane, Abiodun A. Adesiyun, Tiny M. Hlokwe

“…Additionally, 19 environmental samples were collected from feedlots, or where animals drink water while awaiting slaughter, to identify mycobacterial species using culture, acid-fast bacteria staining, and polymerase chain reaction (PCR). The Chi-square and Fisher’s Exact tests were used to detect statistically significant differences in the frequency of detection of Mycobacterium spp. according to the variables investigated (types of tissues, livestock, abattoirs, etc.). …”
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Very Early-Onset Inflammatory Bowel Disease (VEO-IBD) Presenting with Recurrent Leukocytoclastic Vasculitis Preceded by Streptococcal Pharyngitis by Ashley Fonseca, Julee Sunny, Lina M. Felipez

“…Prior to the diagnosis of VEO-IBD, a skin biopsy was obtained and had shown leukocytoclastic vasculitis with a negative IgA stain. Initial laboratory results were remarkable for leukocytosis and increased anti-strep O and anti-DNase B titers. …”
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Basis for the Age-related Decline in Intestinal Mucosal Immunity by Douglas L. Schmucker, Robert L. Owen, Robert Outenreath, Karine Thoreux

“…This was accompanied by a >17% decrease in the areal density of LP blood vessels staining positive for the cell adhesion molecule MAdCAM-1. …”
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Detection of Complement C1q B Chain Overexpression and Its Latent Molecular Mechanisms in Cervical Cancer Tissues Using Multiple Methods by Si-Tong Lin, Zi-Qian Liang, Xiao-Yu Chen, Xin-Qing Ye, Yu-Yan Pang, Jia-Yuan Luo, Jun-Hong Chen, Yi-Wu Dang, Gang Chen

“…In total, 120 cervical cancer tissues, as well as 20 samples each of high-grade squamous intraepithelial lesions (HSILs), low-grade squamous intraepithelial lesions (LSILs), and benign cervical tissue, were collected to evaluate the expression of C1QB protein via immunohistochemical staining. We conducted an integrated analysis of C1QB mRNA expression in cervical cancer using public microarrays and RNA-seq data sets by calculating standard mean differences (SMDs). …”
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Decreased Breg/Th17 Ratio Improved the Prognosis of Patients with Ulcerative Colitis by Xue Bing, Liang Linlang, Chen Keyan

“…Peripheral blood and colonic mucosa were collected from patients with ulcerative colitis. Hematoxylin-eosin staining was used to observe the pathological changes of colonic mucosa. …”
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lncRNA FR215775 Regulates Th2 Differentiation in Murine Allergic Rhinitis by Yue Ma, Le Shi, Keqing Zhao, Chunquan Zheng

“…Then, hematoxylin and eosin, Alcian blue-periodic acid Schiff, and toluidine blue staining were performed, and the levels of IL-2, IL-4, IL-5, IL-6, IL-10, IL-17A, IFN-γ, and TNF in the AR mice were also determined. …”
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