Establishing a Surgical Procedure for Rhesus Epiretinal Scaffold Implantation with HiPSC-Derived Retinal Progenitors by Ziming Luo, Kang Li, Kaijing Li, Bikun Xian, Ying Liu, Sijing Yang, Chaochao Xu, Zhigang Fan, Shoutao Lu, Haijun Zhang, Jian Ge

“…Follow-up examination showed that retinal tack fixation was the only method to maintain the scaffolds attached to host retina for at least 3 weeks, which is the minimal time required for cell integration. Histological staining demonstrated slight glial fibrillary acidic protein (GFAP) accumulation in the retinal tack insertion area. …”
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Differential Effects of Chronic Pulsatile versus Chronic Constant Maternal Hyperglycemia on Fetal Pancreatic β-Cells by Mackenzie S. Frost, Aqib H. Zehri, Sean W. Limesand, William W. Hay, Paul J. Rozance

“…We measured the impact of one week of chronic constant hyperglycemia (CHG, n=6) versus pulsatile hyperglycemia (PHG, n=5) versus controls (n=7) on the percentage of the fetal pancreas staining for insulin (β-cell area), mitotic and apoptotic indices and size of fetal β-cells, and fetal insulin secretion in sheep. …”
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Effect of ectoparasite Ichthyophthirius multifiliis on the histopathology and gill and gut microbiota of goldfish (Carassius auratus) by Xialian Bu, Xialian Bu, Xianqi Peng, Xianqi Peng, Lei Huang, Lei Huang, Yu Zhao, Jinbiao Jiao, Jinbiao Jiao, Jian Zhu, Jing Chen, Jing Chen, Xiaohong Huang, Xiaohong Huang, Aqin Zheng, Aqin Zheng, Huantao Qu, Jiayun Yao, Jiayun Yao

“…However, whether it influences gill and gut microbiota is still unknow.MethodsIn this study, H&E staining was used to show the gill and gut histopathological characteristics of I. multifiliis-infected and uninfected goldfish (Carassius auratus). …”
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Severe Disfiguring Scalp and Facial Oedema due to Henoch–Schönlein Purpura in a Child by Visvalingam Arunath, Arjuna Salinda Athapathu, Thabitha Jebaseeli Hoole, Heshan Aruppala, Asanka Rathnasri, Randula Ranawaka, Sachith Mettananda

“…Urine analysis revealed microscopic haematuria on day 14 of the illness, and immunohistochemical staining of renal biopsy confirmed Henoch–Schönlein purpura nephritis. …”
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Restriction Enzyme Analysis of DNA Methylation in “Condensed” Chromatin of Ha-Ras-Transformed NIH 3T3 Cells by Maria Luiza S. Mello, Ann F. Chambers, Benedicto C. Vidal, Wolfgang Planding, Ulrich Schenck

“…The DNA evaluation was performed by video image analysis in Feulgen‐stained cells previously subjected to treatment with Msp I and Hpa II restriction enzymes, which distinguish between methylated and non‐methylated DNA. …”
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Microscopic Observation of Proliferative Membranes in Fibrocontractive Retinal Disorders by Rino Frisina, Francesco Tessarolo, Ivan Marchesoni, Federico Piccoli, Emiliana Bonomi, Patrizio Caciagli, Edoardo Midena, Giandomenico Nollo

“…Immunohistochemical staining was performed with antibodies to alpha smooth muscle actin and CD68. …”
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The Role of E-Cadherin in Maintaining the Barrier Function of Corneal Epithelium after Treatment with Cultured Autologous Oral Mucosa Epithelial Cell Sheet Grafts for Limbal Stem D... by Fawzia Bardag-Gorce, Richard H. Hoft, Andrew Wood, Joan Oliva, Hope Niihara, Andrew Makalinao, Jacquelyn Thropay, Derek Pan, Imara Meepe, Kumar Tiger, Julio Garcia, Amanda Laporte, Samuel W. French, Yutaka Niihara

“…LSCD corneas did not show continuous staining for ZO-1 or for Cnx43, while CAOMECS-grafted corneas showed a positive expression of ZO-1 and Cnx43. …”
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Melatonin Reverses the Loss of Stemness Induced by TNF-α in Human Bone Marrow Mesenchymal Stem Cells through Upregulation of YAP Expression by Xudong Wang, Tongzhou Liang, Jincheng Qiu, Xianjian Qiu, Bo Gao, Wenjie Gao, Chengjie Lian, Taiqiu Chen, Yuanxin Zhu, Anjing Liang, Peiqiang Su, Yan Peng, Dongsheng Huang

“…The results of colony formation assays, Alizarin red staining, western blotting, and reverse transcription-polymerase chain reactions suggest that melatonin can reverse the inflammatory damage caused by TNF-α treatment in the third, seventh, and tenth generations of primary BMMSCs (vs. control and the TNF-α-treated group). …”
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A single-center prospective study evaluating the relationship of tumor consistency on remission in acromegaly patients by Eren Yilmaz, Seda Duman Ozturk, Ayse Uzuner, Pinar Yildirim, Atakan Emengen, Aykut Gokbel, Melih Caklili, Sibel Balci, Ayca Ersen Danyeli, Burak Cabuk, Ihsan Anik, Savas Ceylan

“…Furthermore, no or weak staining for E-cadherin was associated with Type-2 tumors(p < 0.001). …”
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Tremella fuciformis Polysaccharides Attenuate Oxidative Stress and Inflammation in Macrophages through miR-155 by Yang Ruan, Hong Li, Lianmei Pu, Tao Shen, Zening Jin

“…Reactive oxygen species (ROS) production, real-time PCR, ELISA, and immunofluorescence staining were performed on RAW264.7 cells that were treated with LPS and/or TFPS to investigate the anti-inflammatory effect of TFPS. …”
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The Application of Resolvin D1-Loaded Gelatin Methacrylate in a Rat Periodontitis Model by Zhe Xing, Jing Liu, Jiazheng Cai, Xiaofeng Jiang, Jingwen Liang, Masahito Fujio, Elin Hadler-Olsen, Jing Wang, Alpdogan Kantarci, Ying Xue

“…Periodontal inflammation and tissue regeneration were evaluated using quantitative PCR (qPCR) and histochemical staining, while alveolar bone repair and regeneration were analyzed through micro-CT. …”
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Dynamic changes of extracellular vesicles during zebrafish organogenesis by Linda-Marie Mulzer, Tim Felger, Luis E. Muñoz, Gesa Engl, Heiko Reutter, Mario Schiffer, Leila Pourtalebi Jahromi, Fanni Annamária Boros, Friederike Zunke, Philipp Arnold, Alina C. Hilger

“…EVs were isolated and characterized using flow cytometry, negative staining transmission electron microscopy (TEM), nanoparticle tracking analysis (NTA), and Western Blot. …”
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Examine the Availability and Safety of Mucosal Cutting Biopsy Technique for Diagnosis of Gastric Submucosal Tumor by Kazuhiro Mizukami, Osamu Matsunari, Ryo Ogawa, Yuka Hirashita, Kazuhisa Okamoto, Kensuke Fukuda, Akira Sonoda, Hidetoshi Akiyama, Sotaro Ozaka, Yoshinari Kawahara, Tadayoshi Okimoto, Masaaki Kodama, Kazunari Murakami

“…The pathological diagnosis was made with hematoxylin-eosin staining, with immunostaining added to diagnose GIST. …”
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Stem Cells from Human Exfoliated Deciduous Teeth Attenuate Trigeminal Neuralgia in Rats by Xiaofeng Bai, Xuedi Zhang, Chun Wang, Yao Liu, Xuemei Liu, Yu Fan, Xia Zhang

“…The effects of local SHED transplantation on inflammatory cell infiltration in the trigeminal nerve were investigated based on hematoxylin and eosin staining. The levels of proinflammatory factors in the injured nerve and transient receptor potential vanilloid type 1 (TRPV1) expression in the trigeminal nerve and ganglion were quantified. …”
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Exercise during pregnancy modulates infant cellular and whole‐body adiposity by Alex Claiborne, Filip Jevtovic, Donghai Zheng, Cody Strom, Breanna Wisseman, Samantha McDonald, Edward Newton, Steven Mouro, James DeVente, Joseph A. Houmard, Nicholas T. Broskey, Linda E. May

“…Mesenchymal stem cells (MSC) collected from the umbilical cord at delivery underwent 21 days of adipogenic differentiation, then Oil Red O staining for lipid content. Infant body composition was measured at 1 month of age. …”
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TIM‐4 increases the proportion of CD4+CD25+FOXP3+ regulatory T cells in the pancreatic ductal adenocarcinoma microenvironment by inhibiting IL‐6 secretion by Ziyao Wang, Zerong Xie, Yu Mou, Ruiman Geng, Chen Chen, Nengwen Ke

“…Meanwhile, multiple fluorescent immunohistochemical staining was used to study the distribution characteristics of TIM‐4, and through tissue microarrays, we explored its correlation with patient prognosis. …”
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Stemness Maintenance Properties in Human Oral Stem Cells after Long-Term Passage by Francesca Diomede, Thangavelu Soundara Rajan, Valentina Gatta, Marco D’Aurora, Ilaria Merciaro, Marco Marchisio, Aurelio Muttini, Sergio Caputi, Placido Bramanti, Emanuela Mazzon, Oriana Trubiani

“…Cell proliferation assay, beta galactosidase staining, immunocytochemistry, and real-time PCR techniques were applied. …”
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Studies on the Dual Cytotoxicity and Antioxidant Properties of Berberis vulgaris Extracts and Its Main Constituent Berberine by Lamyae El khalki, Mounir Tilaoui, Abdeslam Jaafari, Hassan Ait Mouse, Abdelmajid Zyad

“…The observed cytotoxicity was related to apoptosis induction as demonstrated by the annexin-V-streptavidin FITC-staining analysis.…”
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Liangxue Tongyu Prescription exerts neuroprotection by regulating the microbiota-gut-brain axis of rats with acute intracerebral hemorrhage by Yingying Sun, Dandan Zhou, Anlan Liu, Yu Zhou, Yang Zhao, Yuan Yuan, Weifeng Guo, Jianxiang Li

“…Analysis via western blot, quantitative real-time PCR, immunohistochemistry (IHC) and tunel staining indicated that LTP upregulated the expression of brain-derived neurotrophic factor (BDNF) and nerve growth factor(NGF) and reduced neuronal cell apoptosis. …”
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Genetic Comparison of Stemness of Human Umbilical Cord and Dental Pulp by Chung-Min Kang, Hyunok Kim, Je Seon Song, Byung-Jai Choi, Seong-Oh Kim, Han-Sung Jung, Seok-Jun Moon, Hyung-Jun Choi

“…Immunohistochemical staining revealed significant differences in the expression of genes (DSPP, DMP1, and CALB1) related to odontogenesis and angiogenesis in DP. …”
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