Induced hepatocyte-like cells derived from adipose-derived stem cells alleviates liver injury in mice infected with Echinococcus Multilocularis by Hui Liu, Xiaojuan Bi, Ning Yang, Xue Zhang, Bingbing Fang, Nuerbaiti Kusuman, Wenmei Ma, Jing Li, Jin Chu, Li Sun, Liang Li, Guodong Lü, Renyong Lin

“…The expression of hepatocyte lineage markers was assessed and iHEPs function was evaluated by Periodic acid-Schiff staining. iHEPs were intravenously transplanted into mice infected with Echinococcus multilocularis. …”
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Primary Nocardia Infection Causing a Fluorodeoxyglucose-Avid Right Renal Mass in a Redo Lung Transplant Recipient by Sreeja Biswas Roy, Mitchell D. Ross, Pradnya D. Patil, Richard Trepeta, Ross M. Bremner, Tanmay S. Panchabhai

“…However, after a right nephrectomy, no evidence of neoplasia was observed; instead, a renal abscess containing filamentous bacteria was noted, raising suspicion for infection of the Nocardia species. Special stains confirmed a diagnosis of Nocardia renal abscess. …”
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Adipose-Derived Stromal Cells Attenuate Adipose Inflammation in Obesity through Adipocyte Browning and Polarization of M2 Macrophages by Wen-Chao Zhang, Feng Qin, Xiao-Jun Wang, Zhi-Fei Liu, Lin Zhu, Ang Zeng, Ming-Zi Zhang, Nan-Ze Yu, Xiao Long

“…In this study, the ADSCs could be stimulated to differentiate into brown adipocyte with rosiglitazone treatment based on the Oil-Red-O staining trial. Furthermore, the multilocular lipid droplets located in the center was increased in differentiated brown adipocytes, and brown fat-associated proteins, UCP1, PPAR-γ, and LPL were highly expressed in brown adipocytes differentiated from ADSCs. …”
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Anticancer, Antioxidant, Antimicrobial Activities, and HPLC Analysis of Alcoholic Extracts of Parthenocissus quinquefolia L. Plant Collected from Çanakkale by Sevil Kalın, Ersin Karabacak, Pınar Ilgın, Alper Önder, Özlem Maraba, Ferah Cömert Önder

“…The cell apoptosis with methanol extract was obtained between 20 µg/mL and 70 µg/mL for 48 h and 72 h treatments by Hoechst 33258 staining of nuclei under a fluorescence microscopy. …”
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Puerarin pretreatment provides protection against myocardial ischemia/reperfusion injury via inhibiting excessive autophagy and apoptosis by modulation of HES1 by Yong Yuan, Songqing Lai, Tie Hu, Fajia Hu, Chenchao Zou, Xiuqi Wang, Ming Fang, Jichun Liu, Huang Huang

“…Additionally, Pue significantly alleviated histopathological damage in MIRI-treated myocardium, as evidenced by HE staining and TUNEL assay. In vitro, Pue pretreatment significantly alleviated A/R-induced damage by decreasing LDH levels, increasing cellular activity, inhibiting autophagic lysosomal overactivation, inhibiting oxidative stress (ROS, LIP ROS, MDA), increasing antioxidant defense (SOD, GSH-Px), and increasing P62 protein expression while decreasing LC3II/I ratio. …”
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Expression and Significance of Immune Checkpoints in Clear Cell Carcinoma of the Uterine Cervix by Liju Zong, Qianqian Zhang, Yuncan Zhou, Yujia Kong, Shuangni Yu, Jie Chen, Youzhong Zhang, Yang Xiang

“…We evaluated the expression of PD-L1, B7-H3, B7-H4, and VISTA in 50 cervical CCCs and 100 SCCs using immunohistochemical staining and investigated the associations between these markers, clinicopathologic features, and survival in patients with CCCs. …”
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Impaired Fasting Glucose and Diabetes as Predictors for Radial Artery Calcification in End Stage Renal Disease Patients by Katarzyna Janda, Marcin Krzanowski, Mariusz Gajda, Paulina Dumnicka, Danuta Fedak, Grzegorz J. Lis, Piotr Jaśkowski, Jan A. Litwin, Władysław Sułowicz

“…Fragments of radial artery obtained during creation of hemodialysis access were stained for calcifications using von Kossa method and alizarin red. …”
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RIPK3-Mediated Necroptosis and Neutrophil Infiltration Are Associated with Poor Prognosis in Patients with Alcoholic Cirrhosis by Zhenzhen Zhang, Guomin Xie, Li Liang, Hui Liu, Jing Pan, Hao Cheng, Hua Wang, Aijuan Qu, Yan Wang

“…Neutrophil infiltration and necroptosis were assessed by immunohistochemical staining for myeloperoxidase (MPO) and RIPK3, respectively. …”
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Antituberculosis Drugs (Rifampicin and Isoniazid) Induce Liver Injury by Regulating NLRP3 Inflammasomes by Qiang Su, Wei Kuang, Weiyi Hao, Jing Liang, Liang Wu, Chunmei Tang, Yali Wang, Tao Liu

“…Histopathological changes that occurred in liver tissue were examined by H&E staining. Additionally, the levels IL-33, IL-18, IL-1β, NLRP3, ASC, and cleaved-caspase 1 expression in the liver tissues were also determined. …”
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Autoimmune pulmonary alveolar proteinosis developed during treatment for systemic sclerosis: a case report by Tomoki Kozono, Kentaro Tanaka, Tomoko Yagi, Kazuto Kamikawaji, Masaki Watanabe, Azusa Iwanaga, Minako Hamada, Yoshifusa Koreeda, Hiromasa Inoue, Ikkou Higashimoto

“…Bronchoscopy showed milky white bronchoalveolar lavage fluid (BALF) and histology of periodic acid-Schiff (PAS) stain-positive eosinophilic granular material. Serum anti granulocyte–macrophage colony-stimulating factor (GM-CSF) antibodies were measured, and the result was positive, leading to the diagnosis of APAP. …”
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Detection of N-Glycolyl GM3 Ganglioside in Neuroectodermal Tumors by Immunohistochemistry: An Attractive Vaccine Target for Aggressive Pediatric Cancer by Alejandra M. Scursoni, Laura Galluzzo, Sandra Camarero, Jessica Lopez, Fabiana Lubieniecki, Claudia Sampor, Valeria I. Segatori, Mariano R. Gabri, Daniel F. Alonso, Guillermo Chantada, María Teresa G. de Dávila

“…Immunoreactivity was moderate to intense in most tumors, showing a diffuse cytoplasmic and membranous staining, although cases of ESFT demonstrated a fine granular cytoplasmic pattern. …”
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The Plant-Derived Bauhinia bauhinioides Kallikrein Proteinase Inhibitor (rBbKI) Attenuates Elastase-Induced Emphysema in Mice by Bruno Tadeu Martins-Olivera, Rafael Almeida-Reis, Osmar Aparecido Theodoro-Júnior, Leandro Vilela Oliva, Natalia Neto dos Santos Nunes, Clarice Rosa Olivo, Marlon Vilela de Brito, Carla Máximo Prado, Edna Aparecida Leick, Mílton de Arruda Martins, Maria Luiza Vilela Oliva, Renato Fraga Righetti, Iolanda de Fátima Lopes Calvo Tibério

“…At day 28, the following analyses were performed: (I) lung mechanics, (II) exhaled nitric oxide (ENO), (III) bronchoalveolar lavage fluid (BALF), and (IV) lung immunohistochemical staining. Results. In addition to decreasing mechanical alterations and alveolar septum disruption, rBbKI reduced the number of cells in the BALF and decreased the cellular expression of TNF-α, MMP-9, MMP-12, TIMP-1, eNOS, and iNOS in airways and alveolar walls compared with the ELA group. rBbKI decreased the volume proportion of 8-iso-PGF2α, collagen, and elastic fibers in the airways and alveolar walls compared with the ELA group. …”
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Effect of Maternal Administration of Edible Bird’s Nest on the Learning and Memory Abilities of Suckling Offspring in Mice by Yong Xie, Hongliang Zeng, Zhiji Huang, Hui Xu, Qunyan Fan, Yi Zhang, Baodong Zheng

“…Additionally, cerebral malondialdehyde (MDA), superoxide dismutase (SOD), choline acetyltransferase (ChAT), and acetylcholinesterase (AChE) in cubs nursed by the female mice given the EBN homogenate were examined, while BDNF immunohistochemical staining and neuron count in hippocampi were investigated as well. …”
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Blockade of Vascular Endothelial Growth Factor Receptor 1 Prevents Inflammation and Vascular Leakage in Diabetic Retinopathy by Jianbo He, Hong Wang, Ying Liu, Wen Li, Dorothy Kim, Hu Huang

“…Vascular leakage was evaluated by measuring the leakage of [3H]-mannitol tracer into the retina and the IF staining of albumin. VEGFR1 blockade significantly inhibited diabetes-related vascular leakage, leukocytes-endothelial cell (EC) adhesion (or retinal leukostasis), expression of intercellular adhesion molecule- (ICAM-) 1 protein, abnormal localization and degeneration of the tight junction protein zonula occludens- (ZO-) 1, and the cell adhesion protein vascular endothelial (VE) cadherin. …”
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Hyperthermia suppresses the biological characteristics and migration of chicken primordial germ cells by Yuzhou Gu, Yuzhou Gu, Yuzhou Gu, Kexin Wu, Kexin Wu, Kexin Wu, Bowen Niu, Bowen Niu, Bowen Niu, Zhiting Wang, Zhiting Wang, Zhiting Wang, Yuchen Jie, Yuchen Jie, Yuchen Jie, Zixuan Fan, Zixuan Fan, Zixuan Fan, Junying Li, Junying Li, Congjiao Sun, Congjiao Sun, Congjiao Sun, Zhuo-Cheng Hou, Zhuo-Cheng Hou, Zhuo-Cheng Hou, Li-Wa Shao, Li-Wa Shao, Li-Wa Shao

“…A gonad-derived PGC line of White Leghorn (WLH) chicken was established and verified through PAS staining and immunofluorescence of PGC-specific proteins. …”
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Nobiletin restores the intestinal barrier of HFD-induced obese mice by promoting MHC-II expression and lipid metabolism by Ni Yang, Yue-Shan Pang, Yali Zheng, Yan-Ju Gong, Wei-Jun Ding

“…Hematoxylin and eosin (HE) staining and immunofluorescence were used to observe the intestinal barrier. …”
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Expression analysis of transforming growth factor beta (TGF-β) in oral squamous cell carcinoma by Arun Ganesh, P.P. Ashikha Shirin Usman, Ameya K.P., Priya Thomas, Dhanraj M. Ganapathy, Durairaj Sekar

“…The collected samples were subjected to staining studies. The RNA was isolated, and expression studies were done using the qRT-PCR method. …”
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Rotavirus VP6 Adjuvant Effect on Norovirus GII.4 Virus-Like Particle Uptake and Presentation by Bone Marrow-Derived Dendritic Cells In Vitro and In Vivo by Kirsi Tamminen, Suvi Heinimäki, Timo Vesikari, Vesna Blazevic

“…The adjuvant effect of VP6 on GII.4 VLP presentation and NoV-specific immune response induction by BMDC in vivo was also studied. Intracellular staining demonstrated that BMDCs internalized both antigens, but VP6 more efficiently than NoV VLPs. …”
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Inhalation of patchouli essential oil alleviates airway inflammation in cigarette smoke-induced COPD mice by Yan Zhang, Rui Cao, Duo Wang, Qiulin Yue, Le Su, Kunlun Li, Baojun Li, Lin Zhao, Song Zhang

“…Histopathological examination revealed that PEO treatment improved lung tissue changes, as observed through staining. Furthermore, PEO treatment reduced the levels of inflammatory factors IL-6, IL-1β, and TNF-α, and reversed the CS-induced elevation of mRNA levels of these factors. …”
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Nasal Carriage of Methicillin-Resistant Staphylococcus aureus among Health Care Workers in Tertiary and Regional Hospitals in Dar es Salam, Tanzania by Agricola Joachim, Sabrina J. Moyo, Lillian Nkinda, Mtebe Majigo, Sima Rugarabamu, Elizabeth G. Mkashabani, Elia J. Mmbaga, Naboth Mbembati, Said Aboud, Eligius F. Lyamuya

“…S. aureus was identified based on colonial morphology, Gram staining, catalase, coagulase, and DNase test results. …”
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