Isolation, Culture, and Characterization of Primary Kidney Fibroblasts from Human Patients with Chronic Antibody-mediated Kidney Transplant Rejection by Mantabya Kumar Singh, Mohit Kumar Rai, Vikas Agarwal, Narayan Prasad

“…The immunofluorescence (IF) staining, shows the primary kidney fibroblast cells at the fourth passage were strongly positive for the fibroblast marker, Collagen I (fibrogenic cells), α-SMA (Resident fibroblast to active myofibroblasts conversion), and Vimentin (mesenchymal cells). …”
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Exploring the anticancer potential of Hewittia malabarica through phytochemical analysis and molecular docking study by Shiji Thozhukkad Moosaripparambil, Kannan Vadakkadath Meethal

“…The cytomorphological changes associated with the chloroform extract and AO/EB dual staining indicated apoptotic cell death. An analysis of ADME properties of compounds obtained in GC-MS analysis of the chloroform extract showed that compounds such as Cyclododecanol, Sulfurous acid hexyl octyl ester, 2,4-Ditert-Butylphenol and 1,2-benzenedicarboxylic acid had favourable physicochemical and drug-likeness characteristics. …”
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Advancing Hydrogel-Based 3D Cell Culture Systems: Histological Image Analysis and AI-Driven Filament Characterization by Lucio Assis Araujo Neto, Alessandra Maia Freire, Luciano Paulino Silva

“…Cross-sections of stretched and unstretched filaments were created and stained with hematoxylin and eosin. Using the Teachable Machine platform, images were grouped and trained for subsequent prediction. …”
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Evaluating the Effect of Active Charcoal-Containing Toothpaste on Color Change, Microhardness, and Surface Roughness of Tooth Enamel and Resin Composite Restorative Materials by Ali Forouzanfar, Pouria Hasanpour, Yasaman Yazdandoust, Hosein Bagheri, Hamideh Sadat Mohammadipour

“…Thirty-six bovine incisors and resin composite samples were prepared, stained with black tea, and then randomly divided into two groups. …”
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Antitumor Activities of Aqueous Cinnamon Extract on 5637 Cell Line of Bladder Cancer through Glycolytic Pathway by Zeynab Aminzadeh, Nasrin Ziamajidi, Roghayeh Abbasalipourkabir, Sajedeh Daei, Sobhan Helbi, Abbas Moridnia

“…Apoptosis was assessed with Hochest33258 staining. For evaluating of aqueous extract of Cinnamon effect on glycolysis, the gene expression of epidermal growth factor receptor 2 (ErbB2), heat shock protein transcription factor1 (HSF1), and lactate dehydrogenase A (LDHA), as well as protein levels of HSF1 and LDHA, LDH activity, glucose consumption, and lactate production, were measured. …”
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Il-6 and MSH3 in colorectal carcinoma: Expression, relationship, and prognostic significance in 171 colorectal carcinoma cases by Elif Kolay Bayram, Betul Ogut, Mehmet Arda Inan, Ayse Dursun

“…Results: A relationship between MSH3 loss and IL-6 expression was not proven (P = 0.963). MSH3 staining was significantly reduced in the patient group with high TILs (P = 0.035). …”
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Effect of ultrasound combined with microbubbles therapy on tumor hypoxic microenvironment by Yuyi Feng, Danxia Qiu, Yangcheng He, Hai Jin, Liping Chen, Fen Xi, Zhiwen Hu, Yanlin Xie, Yucai Li, Minhua Lin, Pengxiao Sun, Yan He, Jianhua Liu

“…The changes in tumor tissue hypoxia before and after treatment were observed by measuring oxygen partial pressure directly with in tumor tissue and immunohistochemical staining for hypoxia-inducible factor-1α (HIF-1α).ResultsResults indicated that low frequency, non-focused ultrasound at 0.5 MPa/20 Hz and 0.5 MPa/40 Hz, when combined with microbubbles, could increase tumor tissue blood perfusion and improve the hypoxia in tumor tissues.DiscussionThis study provides a new method for improving hypoxia in the tumor microenvironment (TME) which could potentially improve the cancer treatments resistance.…”
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RESULTS OF EXPERIMENTAL INFECTION OF TURKEYS WITH A/DUCK/ALTAI/469/14 H5N1 STRAIN OF AVIAN INFLUENZA VIRUS by V. Yu. Sosipatorova, D. A. Altunin, I. A. Chvala, N. S. Mudrak, D. B. Andreychuk

“…One sample was subjected to histological staining using hematoxylin and eosin dyes, and its duplicate was subjected to immunohistochemical assay using a preparation of polyclonal antibodies as primary antibodies against the ribonucleoprotein of avian influenza virus. …”
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Effects of Renal Denervation on Ouabain-Induced Hypertension in Rats by Minna Tang, Jialu Hu, Wenshu Li, Ningzhi Zhang, Sisi Ning, Yan Yan, Zhaoqiang Cui

“…RDN also reduced the thickening of aortic tunica media and downregulated the expression of proliferating cell nuclear antigen (PCNA) in the thoracic aorta induced by ouabain. Masson staining and echocardiography showed that myocardial fibrosis and increased left ventricular mass in the ouabain group could be attenuated by RDN. …”
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Effect of Baicalein on Cognitive Impairment in Diabetes Mellitus with Cerebral Small Vessel Disease by Li ZHOU, Zhengqi LU, Xiaoming YE, Wanxin YANG, Zhisheng WEI, Yi KONG, Xuming HUANG

“…Compared with the model group, the Ach level in the brain homogenate of the treatment group increased significantly (<italic>P</italic>&lt;0.05). The HE staining sections illustrated a large number of active neurons were found in the control group, but loose spongiform degeneration of brain tissue, apoptosis and necrosis of neurons and karyopyknosis were detected in the model group. …”
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Autoantibody to MDM2: A Potential Serological Marker of Systemic Lupus Erythematosus by Yuan Liu, Liping Dai, Weihong Liu, Guixiu Shi, Jianying Zhang

“…These anti-MDM2 positive sera present a nuclear staining pattern. The presence of anti-p53 in SLE patients was 39.50%, and the titer of anti-MDM2 was positively correlated with anti-p53 in SLE patients. …”
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Anatomical variations in Eurasian wolf (Canis lupus lupus) (Carnivora: Canidae) of the salivary glands: A histological and histochemical investigation. by Joanna Klećkowska-Nawrot, Karolina Goździewska-Harłajczuk, Krzysztof Stegmann, Arkadiusz Dziech, Dariusz Łupicki, Gabriela Jędrszczyk, Igor Jucenco, Karolina Barszcz

“…The study involved a gross anatomical description of the parotid gland, mandibular gland, monostomatic sublingual gland, polystomatic sublingual gland, and zygomatic gland in 12 adult Eurasian wolves (Canis lupus lupus) (wild free-ranging individuals and their zoo counterparts), including their morphometry and microscopic evaluation using hematoxylin & eosin, mucicarmine, azan trichrome, PAS, AB pH 1.0, AB pH 2.5; AB pH 2.5/PAS, and HDI stainings. Topographically, the salivary glands were located similarly to those of other terrestrial Carnivora. …”
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Assessment of Immuno-histochemical Expression of CD44 in Renal Tumors by Banan Mohammed, khalid Abdulfattah Khattab, Payman Salim

“…CD44 immunohistochemical stain showed significantly higher expression in cases with higher nuclear grade and tumor stage (P-value of 0.0376 and 0.0075, respectively). …”
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Tumor Budding, uPA, and PAI-1 in Colorectal Cancer: Update of a Prospective Study by Bruno Märkl, Jochen Hardt, Simon Franz, Tina Schaller, Gerhard Schenkirsch, Bernadette Kriening, Reinhard Hoffmann, Stefan Rüth

“…Tumor budding was analyzed on cytokeratin-stained slides. Survival analyses were performed using cut-offs that were determined previously. …”
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Aggressive Papillary Thyroid Carcinoma Presenting with Metastasis to the Pancreas by Firas Warda, Sam Ho, Enoch Kuo, Dinesh Rao, Marilu Jurado-Flores

“…Pathology showed papillary thyroid carcinoma with strong and diffuse staining for TTF-1 and thyroglobulin. The patient was started on lenvatinib in the postoperative period and is currently tolerating treatment well with evidence of decreasing thyroglobulin levels. …”
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Investigation of the osteogenic effects of ICA and ICSII on rat bone marrow mesenchymal stem cells by Zhangshun Yao, Weixiang Huang, Yan Yang, Leiyan Zou, Yunpeng Zhang, Jing Zhang, Guangming Luo

“…Subsequently, we compared the vascularization and osteogenic capacity of the two groups through alizarin red staining assay, Western Blot, and RT-PCR. Subsequently, we assessed the phosphorylated and non-phosphorylated expression of JNK, ERK1/2, P38, and AKT at different time intervals. …”
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Rat Hippocampal Neural Stem Cell Modulation Using PDGF, VEGF, PDGF/VEGF, and BDNF by Neus Gomila Pelegri, Catherine A. Gorrie, Jerran Santos

“…VEGF/PDGF at 100 ng/mL had the greatest influence on cellular proliferation of HNSC, which also stained positively for nestin, OSP, and NF200. In comparison, HNSC in other treatments had poorer cell health and adhesion. …”
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Prostate-Specific Membrane Antigen as Target for Neuroimaging of Central Nervous System Tumors by Brittany M. Stopa, James Crowley, Csaba Juhász, Cara M. Rogers, Mark R. Witcher, Jackson W. Kiser

“…Tissue studies demonstrated PSMA expression in tumor vascular endothelial cells, without expression in normal brain tissue, though the extent and intensity of staining varied by anti-PSMA antibody and methodology. …”
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Phytochemical Analysis and Antibacterial Activity of Stevia Rebaudiana Bertoni Leaves Extract against Streptococcus sanguis (A Primary Inhabitant of Dental Plaque): In Vitro Study by Manar Ibrahim Ahmed, Safa Ali Hamad, Maha Abdulsalam

“…Microscopic examination, gram stain, optochin test, catalase test and polymerase chain reaction were used to ensure the identification of S. sanguis. …”
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ET-1 Promotes Differentiation of Periodontal Ligament Stem Cells into Osteoblasts through ETR, MAPK, and Wnt/β-Catenin Signaling Pathways under Inflammatory Microenvironment by Li Liang, Wei Zhou, Nan Yang, Jifeng Yu, Hongchen Liu

“…The osteogenic potential of PDLSCs was tested using ALP staining. TNF-α, IL-1β, and IL-6 levels were evaluated by ELISA and western blot. …”
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