Deguelin inhibits the proliferation of human multiple myeloma cells by inducing apoptosis and G2/M cell cycle arrest: Involvement of Akt and p38 MAPK signalling pathway by Sun Kening, Chen Ping, Zhang Liang, Lu Zhidong, Jin Qunhua

“…The apoptosis of multiple myeloma cells was studied using AO/EB and Annexin V-FITC/PI staining methods. Multiple myeloma cell cycle analysis was performed through flow cytometry. mRNA expression levels were depicted using qRT-PCR. …”
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Dry eye post-cataract surgery: a systematic review and meta-analysis by Hillary Ta, Paul McCann, Mengli Xiao, Tiffany Lien, Kaleb Abbott, Darren G. Gregory, Riaz Qureshi, Tianjing Li

“…We compared postoperative dry eye outcomes with baseline including Ocular Surface Disease Index (OSDI), tear break up time (TBUT), Schirmer’s I test (ST1), and corneal fluorescein staining (CFS) at short-term (< 1 week) and medium-term (≥ 1 week to 3 months) follow-up. …”
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Morphometric, histometric and histomorphological investigation of the effects of different ratios safflower (chartamus tinctorius l.) oil supplements on glandula uropygialis in jap... by İsmail Demircioğlu, Bestami Yılmaz, Kürşat Filikçi, Gülşah Güngören, Faruk Bozkaya

“…Routine tissue processing was performed for histometric examination, then the tissues were stained with Haematoxylin-Eosin and Sudan Black.<p> <b>Results:</b> The results indicated that safflower oil supplementatio had a significant effect on papillary length, left lobe length, glandular weight, relative glandular weight, glycogen region thickness(p<0.05) and right lobe length with (p<0.01). …”
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Tissue-friendly dentin treatments as a potential element in revascularization protocol (ex-vivo study) by Hala Fayek Khalil, Nadia Ezz El Din Metwalli, Sara Magdy, Mohamed Shamel

“…Osteogenic differentiation was assessed through alizarin red staining for calcium deposition and quantitative PCR analysis of BMP-2, TGF-β1, VEGF, and DSPP gene expression. …”
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Key wound healing genes as diagnostic biomarkers and therapeutic targets in uterine corpus endometrial carcinoma: an integrated in silico and in vitro study by Fuchuan Jiang, Sajjad Ahmad, Sadia kanwal, Yasir Hameed, Qian Tang

“…Validation using TCGA datasets revealed consistent downregulation of these genes in UCEC samples, corroborated by immunohistochemical staining. Promoter methylation analysis showed significantly higher methylation levels in UCEC, correlating with decreased mRNA expression and poor survival outcomes. …”
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Molecular Detection and Multidrug Resistance of Shigella spp. Isolated from Wild Waterfowl and Migratory Birds in Bangladesh by Jarna Karmoker, Md. Saiful Islam, Md. Liton Rana, Md. Ashek Ullah, Fahim Haque Neloy, Nahian Muniath Oishy, Pritom Kumar Pramanik, Mahbubul Pratik Siddique, Sukumar Saha, Md. Tanvir Rahman

“…Shigella spp. were isolated and identified by culturing, staining, and biochemical tests followed by polymerase chain reaction (PCR). …”
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Evaluation of the Effect of Different Glazing Brands on Surface Roughness of Monolithic Zirconia by Riyam Imad Fouad, Lateef Essa Alwan Al Jorani, Abdel Kareem Jasim Khadim Al-Azzawi

“…The samples dimension for the surface roughness test (diameter 10 mm and thickness 2 mm) by using a surface roughness testing machine profilometer (TR200, Germany), divided into 3 groups depending on types of glazing brands materials (VITA, VITA AKZENT® Plus, glaze LT, Germany), the second group with (Ivoclar Vivadent, IPS e.max Ceram glaze and stain liquid, Liechtenstein), and the third group with (GC Initial spectrum glaze liquid, Austria). …”
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A mouse model of immunosuppression facilitates oral Candida albicans biofilms, bacterial dysbiosis and dissemination of infection by Raja Veerapandian, Anuja Paudyal, Sarah M. Schneider, Sonny T. M. Lee, Govindsamy Vediyappan, Govindsamy Vediyappan

“…Results showed abundant growth of resident oral bacteria and a strong C. albicans biofilm on the tongue consisting of hyphae which damaged papillae, the epidermal layer, and invaded tongue tissue with the accumulation of inflammatory cells as demonstrated by Grocott’s methenamine silver and hematoxylin and eosin staining, respectively. The dispersed microbes from the oral biofilm colonized the gastrointestinal (GI) tract and damaged its integrity, disseminating microbes to other organs. …”
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Patient‐Reported Fatigue Associated with Joint Histopathology in Rheumatoid Arthritis by Diyu Pearce‐Fisher, Melanie H. Smith, Bella Y. Mehta, Edoardo Spolaore, Edward DiCarlo, Dongmei Sun, Susan M. Goodman

“…Hematoxylin and eosin stains of sectioned synovium were systematically scored by a pathologist. …”
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Using β-Elemene to reduce stemness and drug resistance in osteosarcoma: A focus on the AKT/FOXO1 signaling pathway and immune modulation by Shaochun Zhang, Zhijie Xing, Jing Ke

“…Bioinformatics analysis, flow cytometry, and immunofluorescence staining were used to evaluate signaling pathway activity and macrophage polarization. …”
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Dissecting macrophage heterogeneity and kaempferol in lung adenocarcinoma: a single-cell transcriptomic approach and network pharmacology by Laiyi Wan, Wentao Hao, Leilei Li, Lin Wang, Yanzheng Song

“…Pseudotime trajectory analysis, immunofluorescence staining, and in vitro assays examined the functional roles of TAMs and the effects of selected compounds on macrophage polarization. …”
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Chemical Composition and Biological Activities of the Essential Oils of Chrysophyllum albidum G. Don (African Star Apple) by Daniel Nartey, Joseph Nana Gyesi, Lawrence Sheringham Borquaye

“…The antibiofilm activity was assessed via the crystal violet staining assay, with Pseudomonas aeruginosa as the model organism. …”
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The Mutual Inhibition of FoxO1 and SREBP-1c Regulated the Progression of Hepatoblastoma by Regulating Fatty Acid Metabolism by Yu Hu, Hongyan Zai, Wei Jiang, Zhenglin Ou, Yuanbing Yao, Qin Zhu

“…The morphology of tumor tissue sections was observed by HE staining. Results. FoxO1 expression was downregulated in HB tissue, while the expressions of SREBP-1c, FASN, ACLY, ACC, and MAGL were upregulated. …”
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Assessment of Myometrial Concentrations of Oestrogen and Progesterone Receptors in the Lower Uterine Segment of Full-Term Pregnancies in Presence or Absence of Labour by Joana Soares de Arruda, Edward Araujo Júnior, Manuel de Jesus Simões, Luiz Kulay Júnior

“…The biopsy of myometrium was realized during caesarian section, and the excised tissue was stained using immunohistochemical techniques for the quantification of the receptors, and with the aid of image-analysis software, the numbers of receptors for each hormone were determined spectrophotometrically. …”
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Effect of decitabine on PD-L2 methylation in whole blood of iodine-induced autoimmune thyroiditis rats by Baiming Jin, Yanbo Qi, Hong Chao, Xiaolei Yang, Hongjie Li, Siyuan Wan

“…After injecting Dec intraperitoneally into EAT rats, we performed arsenic-cerium catalytic spectrophotometry, pathological hematoxylin and eosin staining, enzyme-linked immunosorbent assay, quantitative methylation-specific polymerase chain reaction (qMSP), and quantitative real-time polymerase chain reaction (qPCR) to determine the relevant indices. …”
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Zinc Supplementation Mitigates High Salt Diet-Induced Bone Damage: A Histological Evaluation of Osteocyte Apoptosis by Kaukab Anjum, Abdullah Qamar, Nomana Mahmood, Muhammad Marghoob Khan, Shazia Imran, Ayesha Ali

“…After eight weeks, femurs were harvested, processed, and stained with hematoxylin and eosin. Osteocyte apoptosis was assessed by counting empty lacunae and apoptotic bodies under light microscopy. …”
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LncRNA GAS5 reduces blood glucose levels and alleviates renal fibrosis in diabetic nephropathy by regulating the miR-542-3p/ERBB4 axis by Qinghua Yin, Na Guo, Ruoxi Liao

“…HE, TUNEL and Masson stainings were employed to observe the cellular morphology, apoptosis, and fibrosis of renal tissues, respectively. …”
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Development and evaluation of a novel E7 multi-epitopic vaccine for human papillomavirus type 16: design, expression, purification, and immunological characterization by Bahareh Bahmani, Zahra Amini-bayat, Mohammad Mehdi Ranjbar, Masoud Hassanzadeh Makoui, Amir-Hassan Zarnani

“…Polyclonal antibodies were generated, and their reactivity with immunizing antigen and native protein in E7 expressing cells (TC-1) was assessed by ELISA and immunofluorescent staining, respectively. The efficacy of the vaccine was assessed in a therapeutic animal model of HPV-induced cancer. …”
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Synthesis and protective effect of pyrazole conjugated imidazo[1,2-a]pyrazine derivatives against acute lung injury in sepsis rats via attenuation of NF-κB, oxidative stress, and a... by Zang Binbin, Wang Lihui

“…Results suggested that compound 3h significantly reduced lung inflammation and membrane permeability, as evidenced by H&E staining of lung tissues. It substantially reduced the generation of pro-inflammatory cytokines (TNF-α, IL-1B, IL-6) and oxidative stress (MPO, MDA, SOD). …”
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Extracellular MMP-9-Based Assessment of Ocular Surface Inflammation in Patients with Primary Open-Angle Glaucoma by Anna Zaleska-Żmijewska, Ewa Strzemecka, Zbigniew M. Wawrzyniak, Jacek P. Szaflik

“…Further assessments of disease severity and inflammation comprised Goldmann applanation tonometry for intraocular pressure (IOP), Schirmer’s test with anesthesia, ocular surface staining with unpreserved fluorescein (Oxford scale index), tear breakup time (TBUT), McMonnies questionnaire, and the Ocular Surface Disease index (OSDI). …”
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