The Proapoptotic Effect of MB-653 Is Associated with the Modulation of Metastasis and Invasiveness-Related Signalling Pathways in Human Colorectal Cancer Cells by Libor Sokoli, Peter Takáč, Mariana Budovská, Radka Michalková, Martin Kello, Natália Nosálová, Ľudmila Balážová, Šimon Salanci, Ján Mojžiš

“…The proapoptotic activity was evidenced by the activation of caspases 3 and 7, mitochondrial dysfunction, and an increased number of apoptotic cells, confirmed by annexin V/PI and AO/PI staining. Additionally, MB-653 induces dose-dependent G2/M phase cell cycle arrest, the cause of which could be cyclin B1/CDC2 complex dysfunction and/or a decrease in α-tubulin protein expression. …”
Get full text

Mechanism of hyperbaric oxygen therapy downregulating H-type angiogenesis in subchondral bone of knee osteoarthritis through the PHD2/HIF-1α pathway by Jianjian Wang, Wen Yu, Yuxin Zhang, Bo Chen, Zhaoxiang Meng

“…The effect of hyperbaric oxygen therapy on osteoarthritis was evaluated using Micro-CT, Safranin O-Fast Green staining, and detection of osteoarthritis inflammation markers (MMP-13, ADAMTS-5, Col2a1, and Aggrecan). …”
Get full text

Association of One-Step Nucleic Acid Amplification Detected Micrometastases with Tumour Biology and Adjuvant Chemotherapy by Ghaleb Goussous, Sadaf Jafferbhoy, Niamh Smyth, Lisette Hammond, Sankaran Narayanan, Robert Mark Kirby, Soni Soumian

“…Out of 1285 patients who underwent sentinel node (SLN) biopsy, 76 patients had micrometastases. Using H&E staining, 36 patients were detected with SLN micrometastases (9/year) in contrast to 40 patients in the OSNA year (40/year) (p<0.0001), demonstrating a fourfold increase with the use of OSNA. …”
Get full text

Effect of Repetitive Glucose Spike and Hypoglycaemia on Atherosclerosis and Death Rate in Apo E-Deficient Mice by Kenichi Nakajima, Tomoya Mita, Yusuke Osonoi, Kosuke Azuma, Toshiyuki Takasu, Yoshio Fujitani, Hirotaka Watada

“…Atherosclerosis was evaluated in all mice by oil red O staining. Administration of ipragliflozin, a selective inhibitor of sodium-glucose cotransporter 2, in the mouse model of repetitive glucose spikes inhibited the progression of atherosclerosis, whereas long-term repetitive glucose spikes, repetitive hypoglycaemia, and their combination had no significant impact on atherosclerosis. …”
Get full text

The Fractal Dimension of Nuclear Chromatin as a Prognostic Factor in Acute Precursor B Lymphoblastic Leukemia by Randall L. Adam, Rosana C. Silva, Fernanda G. Pereira, Neucimar J. Leite, Irene Lorand-Metze, Konradin Metze

“…In 28 patients, gray scale transformed pseudo-3D images of 100 nuclei (May–Grünwald–Giemsa stained bone marrow smears) were analyzed. FD was determined by the Minkowski–Bouligand method extended to three dimensions. …”
Get full text

Dopamine Suppresses Osteogenic Differentiation of Rat Bone Marrow-Derived Mesenchymal Stem Cells via AKT/GSK-3β/β-Catenin Signaling Pathway by Zhili Kuang, Zheng Chen, Shaoqin Tu, Zhihui Mai, Lin Chen, Xiaoning Kang, Xiaochuan Chen, Jiaming Wei, Yuxuan Wang, Yun Peng, Hong Ai

“…Interestingly, dopamine suppressed the osteogenic differentiation of rBMSCs, as characterized by reduced ALP staining, ALP activity, mineralized nodule formation, and the mRNA and protein levels of osteogenesis-related genes (Col1a1, Alp, Runx2, Opn, and Ocn). …”
Get full text

Inactivation of antibiotic resistant bacteria by nitrogen-doped carbon quantum dots through spontaneous generation of intracellular and extracellular reactive oxygen species by Weibo Xia, Zixia Wu, Bingying Hou, Zhang Cheng, Dechuang Bi, Luya Chen, Wei Chen, Heyang Yuan, Leo H. Koole, Lei Qi

“…The antimicrobial activity was confirmed by live/dead staining. In in vivo studies, the N-CQDs were more efficient in treating drug-resistant bacterial keratitis and reducing corneal damage compared to the common antibiotic levofloxacin. …”
Get full text

L-theanine promotes angiogenesis in limb ischemic mice by modulating NRP1/VEGFR2 signaling by Jingyi Wang, Yinghui Xu, Yating Ruan, Xinyang Hu

“…Blood flow recovery was measured via Doppler ultrasound, and vascular density was analyzed using immunofluorescence staining. RNA sequencing identified neuropilin-1 (NRP1) as a key regulator, and the expression levels of NRP1 and VEGFR2 were examined through qPCR and Western blotting. …”
Get full text

Current Status of Fasciolosis of Goat in Sylhet, Bangladesh: An Integrated Morphomolecular Study by Chamali Akter Shykat, Saiful Islam, Foyez Ahmed, Kazi Mehetazul Islam, Jamal Uddin Bhuiyan, Tilak Chandra Nath

“…Collected flukes were examined by light microscopy after being stained with Semichon’s acetocarmine, and sequences of mtDNA Cox1 genes were obtained. …”
Get full text

The Effect of Clopidogrel and Ticagrelor on Human Adipose Mesenchymal Stem Cell Osteogenic Differentiation Potential: In Vitro Comparative Study by Sally S. Mohamed, Hala F. Zaki, Shereen N. Raafat

“…The osteogenic differentiation potential was determined by mineralization nodule formation using Alizarin Red S staining, measuring ALP enzyme activity by alkaline phosphatase assay. …”
Get full text

Effects of Earthworm (Pheretima communisima) extract on atopic dermatitis: An in vitro and in vivo study by Yoon-Young Sung, Heung-Joo Yuk, Seung-Hyung Kim, Dong-Seon Kim

“…Immunohistofluorescence staining of skin lesions from AD mice revealed that EWE induced expression of claudin-1, filaggrin, and SIRT1. …”
Get full text

Induction of somatic embryogenesis and cryopreservation of Abies pinsapo Boiss. by Laura Cabero-Moreno, Ignacio Landeras-López, María Victoria Bravo-Navas, Carolina Sánchez-Romero

“…Following fluorescein diacetate (FDA) staining, more intense and abundant green fluorescence could be observed in these samples, compared to those subjected to other preconditioning and cryoprotective treatments, thus evincing a higher proportion of viable cells after freezing in liquid nitrogen. …”
Get full text

Single-cell RNA sequencing reveals tumor heterogeneity in small cell neuroendocrine cervical carcinoma by Xuesong Xiang, Xiang Tao, Keqin Hua, Hua Jiang, Jingxin Ding

“…Based on immunohistochemical staining of malignant subtyping markers, a cohort of 66 SCNECC patients from our hospital were divided into five subtypes. …”
Get full text

Spectroscopic, Diagnostics, and Surface Modification of Stainless Steel Doped with Ti Plasma Produced by DC Magnetron Sputtering and Used in Biomedical Applications by Mustafa A. Mahmood, Riyam Adnan Hammudi

“…SEM-EDS analyses confirm the roughness of the surface and elemental composition of stain steel doping with titania with 4.5 eV peak and 6.5eV peak. …”
Get full text

Comparison of Prevalence of blaNDM-1 Gene among Metallo-Beta Lactamase-Producing Multidrug-Resistant Fermenters and Nonfermenters in a Tertiary Care Hospital by Shaveta Kataria, Shipra Galhotra, Mohit Kumar, Priyanka Thandi, Neerja Jindal

“…Brief history and related data of the patients was being recorded. The direct Gram staining and samples were inoculated on culture media and incubated at 37°C for 18–24 h. …”
Get full text

Ocular Surface Disease Severity in Polycystic Ovary Syndrome: is This Really Significant in Daily Life? by Demet Yabanoglu, Funda Gode

“…Ocular surface disease grading was performed using the Ocular Surface Disease Index questionnaire, slit lamp examination, meibomian gland secretion scoring, tear film breakup time, staining scores of ocular surfaces, the Schirmer's test, and tear osmolarity assessment. …”
Get full text

Differential expression of “A Disintegrin and Metalloproteinase 10” in hepatocellular carcinoma and the noncancerous hepatic tissues: Contribution to HCV-promoted hepatocarcinogene... by Amal Abd El Hafez, Basem H. Elesawy, Heba S. E. I. Hany

“…Materials and Methods: IHC staining for anti-ADAM10 was performed using horseradish peroxidase technique. …”
Get full text

Histological Changes of the Mucosal Epithelium in the Chicken Intestine during Pre- and Post-Hatching Stages by Md. Al amin, Md Badiul Alam, Kohzy Hiramatsu

“…Paraffin sections prepared from tissue samples were stained with periodic acid-Schiff followed by alcian blue for histological analysis and to detect goblet cells. …”
Get full text

Reparative histogenesis of bone tissue in femoral fractures in rats using biocomposite material along with immunocorrection by V. V. Reshetnyak, V. V. Burdeyniy, V. V. Pronin, Ye. A. Iskaliev

“…The connective tissue was stained more intensely which indicates a denser arrangement of fibers in the callus. …”
Get full text

Empagliflozin Alleviates Hepatic Steatosis by Activating the AMPK-TET2-Autophagy Pathway in vivo and in vitro by Ting Li, Ting Fang, Linxin Xu, Xiangyang Liu, Xiaoyu Li, Mei Xue, Xiaochen Yu, Bei Sun, Liming Chen

“…Hepatic steatosis was evaluated by Oil Red O staining and triglyceride quantification. Immunohistochemistry, immunofluorescence, and western blot were performed to assess protein levels.Results: Empagliflozin alleviated liver steatosis in db/db mice and reduced triglyceride content and lipid accumulation in the hepatocyte steatosis model. …”
Get full text