A Case of Mucosal Cancer of the Stomach Treated by Endoscopic Submucosal Dissection after Which Nodal Metastasis Became Evident by Takashi Obana, Naotaka Fujita, Yutaka Noda, Dai Hirasawa, Kei Ito, Toshiki Sugawara, Yoshihiro Harada, Tetsuya Oohira, Hiroshi Honda, Takashi Sawai

“…Because lymphatic-vascular involvement was not detected by hematoxylin and eosin (HE) staining, additional gastrectomy was not performed. …”
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Immunocytochemical and Morphometric Studies of Gastrin-, Somatostatin- and Serotonin-Producing Cells in the Stomach and Duodenum of Patients with Acid Peptic Disorders by WR Yacoub, ABR Thomson, P Hooper, LD Jewell

“…Seven patients with minimal dyspeptic symptoms and an endoscopically and histologically normal stomach and duodenum served as controls. Immunoperoxidase staining for gastrin-producing G cells, somatostatin-producing D cells and serotonin-producing EC cells was carried out on fundic, antral and duodenal biopsies, and was quantified using a Zeiss MOP Videoplan using the peroxidase-antiperoxidase technique of Sternberger. …”
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Biological evaluation of novel 5-((1H-indol-3-yl) methyl)-2-(4-chlorobenzyl)-6-phenyl-imidazo[2,1-b][13,4]thiadiazole derivatives as novel BCL-2 specific inhibitors by Shivangi Sharma, Sadashivamurthy Shamanth, Humaira Siddiqua, Laijau Goyary, Kunigal S. Sagar, Susmita Kumari, Divya Sathees, Gudapureddy Radha, Bibha Choudhary, Sathees C. Raghavan, Kempegowda Mantelingu

“…Western blot analysis in tandem with Annexin V/PI staining confirmed the activation of apoptosis. In silico studies, using derivatives suggests a promising therapeutic window with less off-target effects. …”
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Transcutaneous Electrical Acupoint Stimulation in Early Life Changes Synaptic Plasticity and Improves Symptoms in a Valproic Acid-Induced Rat Model of Autism by Xiaoxi Wang, Rui Ding, Yayue Song, Juan Wang, Chen Zhang, Songping Han, Jisheng Han, Rong Zhang

“…Additional studies demonstrated that the dendritic spine density of the primary sensory cortex was decreased with Golgi staining. Results of the transcriptomic study showed that expression of some transcription factors such as the neurotrophic factor were downregulated in the hypothalamus of the VPA model of ASD. …”
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Human Adipose-Derived Mesenchymal Stem Cells Cryopreservation and Thawing Decrease α4-Integrin Expression by Ana Carolina Irioda, Rafael Cassilha, Larissa Zocche, Julio Cesar Francisco, Ricardo Correa Cunha, Priscila Elias Ferreira, Luiz Cesar Guarita-Souza, Reginaldo Justino Ferreira, Bassam Felipe Mogharbel, Venkata Naga Srikanth Garikipati, Daiany Souza, Mirian Perlingeiro Beltrame, Katherine Athayde Teixeira de Carvalho

“…Before and after cryopreservation the immunophenotype, cellular viability analysis by flow cytometer, colony forming units ability, differentiation potential into adipocytes and osteoblasts as demonstrated by Oil Red O and Alizarin Red staining, respectively. Results. The immunophenotypic markers expression was largely preserved, and their multipotency was maintained. …”
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Therapeutic potential of hydrogen-rich water in zebrafish model of Alzheimer’s disease: targeting oxidative stress, inflammation, and the gut-brain axis by Jiaxuan He, Peiye Xu, Ting Xu, Haiyang Yu, Lei Wang, Rongbing Chen, Kun Zhang, Yueliang Yao, Yanyan Xie, Qinsi Yang, Wei Wu, Da Sun, Dejun Wu

“…Behavioral assays, ELISA, Hematoxylin–eosin (H&E) staining, and reactive oxygen species (ROS) and neutrophil fluorescence labeling were employed to assess HRW’s impact. …”
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Comparison of the Manual, Semiautomatic, and Automatic Selection and Leveling of Hot Spots in Whole Slide Images for Ki-67 Quantification in Meningiomas by Zaneta Swiderska, Anna Korzynska, Tomasz Markiewicz, Malgorzata Lorent, Jakub Zak, Anna Wesolowska, Lukasz Roszkowiak, Janina Slodkowska, Bartlomiej Grala

“…The samples were immunohistochemically stained to determine the Ki-67/MIB-1 proliferation index used for prognosis and treatment planning. …”
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Genome Structure of Bacillus cereus tsu1 and Genes Involved in Cellulose Degradation and Poly-3-Hydroxybutyrate Synthesis by Hui Li, Suping Zhou, Terrance Johnson, Koen Vercruysse, Ouyang Lizhi, Parthasarathy Ranganathan, Nsoki Phambu, Alexander J. Ropelewski, Theodore W. Thannhauser

“…The PHB accumulation in B. cereus tsu1 was initially identified using Sudan Black staining and then confirmed using high-performance liquid chromatography. …”
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Therapeutic Effect of Intense Pulsed Light (IPL) Combined with Meibomian Gland Expression (MGX) on Meibomian Gland Dysfunction (MGD) by Shanshan Wei, Xiaotong Ren, Yuexin Wang, Yilin Chou, Xuemin Li

“…Meibomian gland assessment scores, including meibum quality and expressibility, eyelid margin abnormalities, and corneal staining, significantly decreased after treatment (P<0.05). …”
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Active Pedicle Epithelial Flap Transposition Combined with Amniotic Membrane Transplantation for Treatment of Nonhealing Corneal Ulcers by Ting Zhang, Yuexin Wang, Yanni Jia, Dongle Liu, Suxia Li, Weiyun Shi, Hua Gao

“…Postoperatively, ulcer healing time was detected by corneal fluorescein staining. Visual acuity, intraocular pressure, surgical complications, and recurrence were recorded. …”
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Ziziphus jujube promotes fertility and pregnancy outcomes in Rat model of uterine adhesions by Fereshteh Asgharzadeh, Fereshteh Asgharzadeh, Mahsa Attarian, Majid Khazaei, Majid Khazaei, Abdulridha Mohammed Al-Asady, Abdulridha Mohammed Al-Asady, Abdulridha Mohammed Al-Asady, Saeide Mansoori, Hamideh Naimi, Moein Eskandari, Azar Khorrami, Seyedeh Elnaz Nazari, Akram Aminian, Marjaneh Farazastanian, Elaheh Eshtad, Amir Avan, Amir Avan, Mikhail Ryzhikov, Malihe Hasanzadeh, Seyed Mahdi Hassanian, Seyed Mahdi Hassanian

“…Fibrotic changes were examined through histological Trichrome staining and analysis of pro-fibrotic factors.ResultsTreatment with Z. jujube resulted in a significant reduction in uterine tissue fibrosis, as evidenced by histological evaluation and reduced expression of fibrotic markers. …”
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Comparing Efficacies of Biopsy and Rapid Urease Testing for H. Pylori at a Single Institution by Kathleen Hill, Aiden V. Ohning, Jonathan P. Mallory, Stella Self, Christine M. G. Schammel, Jenny Meredith, John Reddic, Patrick Kent, Jenny B. Knight

“…We sought to evaluate the efficacy of H. pylori invasive detection methods: stained biopsy and rapid urease test (RUT) at a single institution. …”
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Hematopoietic Stem and Progenitor Cell Expansion in Contact with Mesenchymal Stromal Cells in a Hanging Drop Model Uncovers Disadvantages of 3D Culture by Olga Schmal, Jan Seifert, Tilman E. Schäffer, Christina B. Walter, Wilhelm K. Aicher, Gerd Klein

“…After one week, however, HSPC attachment increased considerably, leading to spheroid collapse as demonstrated by electron microscopy and immunofluorescence staining. In terms of HSPC proliferation, the conventional 2D coculture system was superior to the hanging drop model. …”
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Morphometric Evaluation of Interrenal Gland and Kidney Macrophages Aggregates in Normal Healthy Rainbow Trout (Oncorhynchus mykiss) and after Bacterial Challenge with Yersinia ruck... by Michela Gregori, Vincenzo Miragliotta, Roberto Leotta, Stefano Cecchini, Marino Prearo, Francesca Abramo

“…The study presented herein investigates the quantitative modifications from normal anatomical condition, of interrenal gland (IG) and kidney MA in six treatment groups of adult rainbow trout submitted to either specific or aspecific immune stimulation and subsequently challenged with Yersinia ruckeri. Routinely stained tissue sections from both IG and kidney were analysed. …”
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An Immunocompetent HIV-Negative Elderly Patient with Low-Grade Fever, Generalized Lymphadenopathy, Splenomegaly, and Acute Phase Response: Do Not Forget Castleman Disease by Kalliopi Azariadis, Maria Ioannou, Kalliopi Zachou, George N. Dalekos

“…Of note, the outer-zone plasmablasts’ nuclei stained positive for human herpesvirus-8 (HHV8). The patient received 4 cycles of cyclophosphamide, vincristine, and dexamethasone with regression of all symptoms. …”
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Inhibition of P38 MAPK Downregulates the Expression of IL-1β to Protect Lung from Acute Injury in Intestinal Ischemia Reperfusion Rats by De-Yi Zheng, Min Zhou, Jiao Jin, Mu He, Yi Wang, Jiao Du, Xiang-Yang Xiao, Ping-Yang Li, Ai-Zhu Ye, Jia Liu, Ting-Hua Wang

“…Here, we found that the level of pulmonary edema at 16 hours after operation (hpo) was obviously enhanced compared to that in 8hpo and sham groups. Immunofluorescent staining demonstrated that IL-1β and P38 MAPK were detected in lung tissues. …”
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Association of JMJD2B and Hypoxia-Inducible Factor 1 Expressions with Poor Prognosis in Osteosarcoma by Xujian Liu, Qianqian Zhang, Yi Zhao, Jianjun Xun, Hongzeng Wu, Helin Feng

“…JMJD2B and HIF1 were examined by immunohistochemical staining of paraffin-embedded osteosarcoma samples, and their association with clinical characteristics was examined by Spearman’s test. …”
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Changes in T-Cell and Monocyte Phenotypes In Vitro by Schistosoma mansoni Antigens in Cutaneous Leishmaniasis Patients by Aline Michelle Barbosa Bafica, Luciana Santos Cardoso, Sérgio Costa Oliveira, Alex Loukas, Alfredo Góes, Ricardo Riccio Oliveira, Edgar M. Carvalho, Maria Ilma Araujo

“…Peripheral blood mononuclear cells of CL patients were cultured with L. braziliensis antigen in the presence or absence of the S. mansoni antigens rSm29, rSmTSP-2- and PIII. Cells were stained with fluorochrome conjugated antibodies and analyzed by flow cytometry. …”
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An Experimental Study on Botulinum Toxin Type A for the Treatment of Excessive Secretion after Submandibular Gland Transplantation in Rabbits by Shang Xie, Hui Xu, Bo Lin, Kan Wang, Xiao-Feng Shan, Zhi-Gang Cai

“…Hematoxylin and eosin (HE) staining, transmission electron microscopy (TEM), Western blot, and immunofluorescence were performed to analyze its possible mechanisms. …”
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Generation of Femtosecond Laser-Cut Decellularized Corneal Lenticule Using Hypotonic Trypsin-EDTA Solution for Corneal Tissue Engineering by Man-Il Huh, Kyoung-Pil Lee, Jeongho Kim, Soojin Yi, Byeong-Ung Park, Hong Kyun Kim

“…Of all protocols, the decellularization methods, which efficiently removed nuclear materials examined as detected by immunofluorescent staining, were quantitatively tested for sample DNA and glycosaminoglycan (GAG) contents, recellularization efficacy, and biocompatibilities. …”
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