Preparation and in vitro biological evaluation of strontium doped β-tricalcium phosphate scaffolds for alveolar bone tissue regeneration by Congrui Liu, Guiyong Xiao, Zichen Wang, Mengchen Xu, Yinchuan Wang, Nan Wang, Zhenlong Sun, Xiaoyan Li, Yixin Yin

“…The alkaline phosphatase assay and mineralization staining confirmed the osteogenic differentiation potential of Srx-TCP in vitro, particularly evident in the Sr8-TCP scaffold. …”
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Vitrification affects the post-implantation development of mouse embryos by inducing DNA damage and epigenetic modifications by Yurong Chen, Haibo Zhu, Fucheng Guo, Luyao Wang, Wenli Zhang, Ruizhi Liu, Xiaoling Zhang, Xiangpeng Dai

“…The warmed embryos which were frozen at 8-cell stage were cultured in vitro until the blastocyst stage and were transferred into recipients. Immunofluorescence staining was performed to evaluate the reactive oxygen species (ROS) level, mitochondrial function, cell apoptosis, DNA damage and histone epigenetic modification in blastocysts. …”
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Trifarotene alleviates skin photoaging injury by inhibition of JNK/c-Jun/MMPs by Fei Xuan, Yang Lele Zixin, Zhang Jingjing, Li Xiang, Pan Mengtian, Xu Guangchen, Zhang Cuixia, Liu Fei, Fang Weirong

“…This study aimed to investigate the therapeutic effects and mechanisms of trifarotene, a functional retinoic acid analog, on UV-irradiated photoaging ICR and BALB/c nude mice and UVB photodamaged human epidermal keratinocyte (HaCaT) cells by examining indicators such as collagen, oxidoreductase, and inflammatory factor presence through histochemical staining, Western blot, and ELISA. Results suggested that trifarotene significantly reduced UV-induced photoaging in mouse skin tissue, potentially by reducing oxidative stress damage and inflammatory factor release, and inhibiting melanin deposition and collagen degradation by downregulating MMP expression. …”
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Revisiting the Synergistic In Vitro Antimicrobial and Antibiofilm Potential of Chlorhexidine Gluconate and Cetrimide in Combination as an Antiseptic and Disinfectant Agent by Diamond Jain, Rimjhim Gupta, Rashmi Mehta, Pratheesh N. Prabhakaran, Deva Kumari, Kulpreet Bhui, Deepa Murali

“…The synergism was assayed by determining the fractional inhibitory concentrations, while the antibiofilm effects were determined using crystal violet staining and the resazurin assay. Further, the effects on the biofilms were visualized using brightfield and confocal laser scanning microscopy. …”
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Retinoic Acid Signal Negatively Regulates Osteo/Odontogenic Differentiation of Dental Pulp Stem Cells by Jiangyi Wang, Guoqing Li, Lei Hu, Fei Yan, Bin Zhao, Xiaoshan Wu, Chunmei Zhang, Jinsong Wang, Juan Du, Songlin Wang

“…Alkaline phosphatase (ALP) activity assays, alizarin red staining, quantitative calcium analysis, CCK8 assay, osteogenesis-related gene expression, and in vivo transplantation were conducted to determine the osteo/odontogenic differentiation potential and proliferation potential of DPSCs. …”
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Lipopolysaccharide-Binding Protein Downregulates Fractalkine through Activation of p38 MAPK and NF-κB by Xia Huang, Yi Zeng, Yujie Jiang, Yueqiu Qin, Weigui Luo, Shulin Xiang, Suren R. Sooranna, Liao Pinhu

“…RT-PCR, western blotting, ELISA, immunofluorescence, coimmunoprecipitation, and immunohistochemical staining were used to study the expression of fractalkine and LBP and p38 MAPK and p65 NF-κB activities. …”
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Extracellular matrix stiffness regulates colorectal cancer progression via HSF4 by Kangtao Wang, Siyi Ning, Shuai Zhang, Mingming Jiang, Yan Huang, Haiping Pei, Ming Li, Fengbo Tan

“…Tumour stiffness was assessed using magnetic resonance elastography (MRE), and collagen ratio was analysed with Masson staining. CRC cell lines were cultured on matrices of varying stiffness, followed by transcriptome sequencing to identify stiffness-related genes. …”
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Molecular evidence for the efficacy of PBM therapy in the treatment of oral lichen planus by Maria Zaharieva Mutafchieva, Milena Nenkova Draganova, Plamen Ivanov Zagorchev, Reem Hanna, Georgi Tomchev Tomov

“…OLP tissue sections demonstrated lower staining for bcl-2 and Ki-67 compared to healthy controls. …”
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Effectiveness of purple leaves (Graptophyllum pictum L. Griff) and hydroxyapatite as socket preservation biomaterials by Ratri Maya Sitalaksmi, Rizkipriyanto Azharpratomo, Shafira Ninditya Irsan, Primanda Nur Rahmania, Sisca Meida Wati, Syafira Salsabila Adam, Kavanila Bilbalqish, Khairul Anuar bin Shariff, Harry Laksono, Agus Dahlan, Devi Rianti

“…Method: This research used MTT Assay, ICC, and Alizarin Red staining. The study groups were as follows: Group 1: Ad-MSCs; Group 2: Ad-MSCs + αmem; Group 3: Ad-MSCs + osteogenic medium + 1 % nano-extract of Purple leaves & Hydroxyapatite; Group 4: Ad-MSCs + osteogenic medium + 2 % nano-extract of Purple leaves & Hydroxyapatite. …”
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Protective Effects of Necrostatin-1 against Concanavalin A-Induced Acute Hepatic Injury in Mice by Yingqun Zhou, Weiqi Dai, Chunlei Lin, Fan Wang, Lei He, Miao Shen, Ping Chen, Chenfen Wang, Jie Lu, Ling Xu, Xuanfu Xu, Chuanyong Guo

“…The expression of TNF-α, RIP1, and LC3 was detected with immunohistochemical staining. The expression of TNF-α, IFN-γ, IL2, IL6, caspase 3, RIP1, beclin-1, and LC3 protein was assessed by immunofluorescence and western blotting. …”
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Impact of Long-term Polyvinylpyrrolidone Exposure on Gene Expression, Chromatin Structure, and Malondialdehyde Levels in Human Sperm by Mojdeh Sabour, Mahmood Dehghani Ashkezari, Azam Agharahimi, Seyed Morteza Seifati, Seyed Mehdi Kalantar

“…This study was designed to evaluate the effects of this component on chromatin quality with acridine orange (AO) staining, Malondialdehyde (MDA) levels, and BAX, BCL2, HSP70 (HSPA2), and SOD2 genes expression in different time intervals (0, 15, 30, and 60 minutes). …”
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Modulation of Caecal Microbiome in Obese Mice Associated with Administration of Amaranth or Soybean Protein Isolates by Diana Olguín-Calderón, Jorge L. González-Escobar, Rosalva Ríos-Villa, Elena Dibildox-Alvarado, Antonio De León-Rodríguez, Ana P. Barba de la Rosa

“…Morphological changes in caecum ultrathin sections were measured after hematoxylin/eosin staining. Microbiota was isolated from the caecum and 16S rRNA gene was sequenced. …”
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Activation of Opioid Receptors Attenuates Ischemia/Reperfusion Injury in Skeletal Muscle Induced by Tourniquet Placement by Yue-Xian Guo, Gui-Ying Wang, Wen-jie Cheng, Cai-Zhen Yan, Shuang Zhao, Zhao Li, Peng Liu, Xiu-Li Wang

“…Morphological changes of the gastrocnemius muscle in these mice were assessed by hematoxylin-eosin (HE) staining and electron microscopy. Expression levels of TLR4, NF-κB, SIRT1, and PGC-1α in the skeletal muscles were detected by western blot. …”
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TrkB Receptor Antagonism Enhances Insulin Secretion and Increases Pancreatic Islet Size in Rats Fed a Cafeteria-Style Diet by Jorge Agustín Velasco-Gutierrez, Elena Roces de Alvarez-Buylla, Sergio Montero, Alejandrina Rodríguez-Hernández, Saraí Limón Miranda, Karmina Martínez-Santillan, María del Rosario Álvarez-Valadez, Mónica Lemus, Alejandra Flores-Silva, Adolfo Virgen-Ortiz

“…After 4 months of intervention, the glucose and insulin tolerance curves, serum insulin levels, body fat and hematoxylin-eosin staining pancreas were evaluated. <b>Results:</b> The results showed that the cafeteria-style diet induced an increase in the amount of body fat, alterations in the glucose tolerance curve, increased insulin circulation levels, increased HOMA indices and increased pancreatic islet size. …”
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Immunophenotypic Characterization of Human Bone Marrow Mast Cells. A Flow Cytometric Study of Normal and Pathological Bone Marrow Samples by Luis Escribano, Alberto Orfao, Jesús Villarrubia, Beatriz Díaz-Agustín, Carlos Cerveró, Agustín Rios, José L. Velasco, Juana Ciudad, José L. Navarro, Jesús F. San Miguel

“…The goal of the present paper was to define the immunophenotype of bone marrow mast cells (BMMC) from healthy controls and patients with hematologic malignancies (HM) based on the use of multiple stainings with monoclonal antibodies analyzed by flow cytometry. …”
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Mechanical Strain-Mediated Tenogenic Differentiation of Mesenchymal Stromal Cells Is Regulated through Epithelial Sodium Channels by Hui Yin Nam, Malliga Raman Murali, Raja Elina Ahmad, Belinda Pingguan-Murphy, Hanumantha Rao Balaji Raghavendran, Tunku Kamarul

“…Analyses at these time points included cell morphology and alignment observation, immunocytochemistry and immunofluorescence staining (collagen I, collagen III, fibronectin, and N-cadherin), and gene expression (ENaC subunits, and tenogenic markers). …”
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Dual inhibition of BET and EP300 has antitumor activity in undifferentiated pleomorphic sarcomas and synergizes with ferroptosis induction by Stéphanie Verbeke, Aurélien Bourdon, Mathilde Lafon, Vanessa Chaire, Bertolo Frederic, Amina Naït Eldjoudi, Marie-Alix Derieppe, Francis Giles, Antoine Italiano

“…The most potent inhibitor was evaluated in vivo in two animal models and the mechanisms of action were investigated by RNA sequencing, Western blotting and immunofluorescence staining. A CRISPR knockout screen was performed to identify resistance mechanisms. …”
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Human parietal epithelial cells as Trojan horses in albumin overload by Giovanna Priante, Monica Ceol, Lisa Gianesello, Claudia Maria Radu, Rachele Mantese, Lucia Federica Stefanelli, Martina Cacciapuoti, Francesca K. Martino, Lorenzo Arcangelo Calò, Franca Anglani, Federico Nalesso, Dorella Del Prete

“…Cell morphology was analyzed by phase-contrast microscopy and F-actin staining. We demonstrate that hPECs internalize albumin via receptor-mediated mechanisms. …”
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Interleukin 10 Plays an Important Role in Neonatal Rats with Hypoxic-Ischemia Associated with B-Cell Lymphoma 2 and Endoplasmic Reticulum Protein 29 by Xue Bai, Liu-Lin Xiong, Chang-Le Fang, Hao-Li Zhou, Lu-Lu Xue, Yue Hu, Qing-Jie Xia, Jia Liu, Jun-Yan Zhang, Ting-Hua Wang, Si-Jin Yang

“…Immunofluorescence double staining was performed to observe the localization of IL-10 in neurons and astrocytes. …”
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Epigallocatechin-3-gallate inhibits the collagen accumulation of oral submucous fibrosis induced by arecoline by Ge Gao, Ge Gao, Caipeng Lin, Caipeng Lin, Ruibo Li, Xi Xie, Hai-Bin Luo, Hai-Bin Luo

“…The study also established an OSF model in Sprague-Dawley rats, induced by ARE, with pathological changes characterized using HE and Masson’s staining, further assessing the impact of ARE on ECM-related protein expression in rat oral tissues through RT-qPCR and Western blot methods.ResultsEGCG effectively suppressed the ARE-induced ECM components while concurrently improving the OSF pathological process in vitro and in vivo.ConclusionThe results indicate that the natural product EGCG effectively suppressed the increased ECM components induced by ARE and concurrently improved the OSF pathological process, indicating that EGCG could be potentially a novel anti-fibrotic candidate drug for the treatment of OSF.…”
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