Histopathological analysis of the liver in hypercholesterolemia rats treated with Dillenia serrata fruits by Tien, Tri Wistya Utami, Pranita Aritrina, Laode Kardin, Sukurni, Andi Noor Kholidha Syarifin

“…Rat liver tissues were analyzed histologically using Hematoxylin-Eosin (HE) staining, and were observed under a light microscope at 40x magnification in five wide fields of view. …”
Get full text

Tertiary Lymphoid Structure in Dental Pulp: The Role in Combating Bacterial Infections by Ruiqi Li, Fan Gu, Linlin Peng, Tingting Huan, Zhuo Zhou, Yaling Song, Jinmei He, Kaili Ye, Yao Sun, Tiejun Li, Miao He, Zhuan Bian, Wei Yin

“…Multiple immunofluorescence staining reveals that typical TLS emerges in dental pulp with pulpitis, consistent with the high expression of CC chemokine ligand 3 (CCL3), which may be a key driver of TLS formation. …”
Get full text

A Rare Case of Pneumonia Caused by Shewanella putrefaciens by Rajshree Patel, Albin Abraham, Johnson Thomas, Wanqing Zhi, Shadab Ahmed, Janice Verley

“…Empirical antibiotic treatment with vancomycin and piperacillin/tazobactam combination was initiated. Gram-stained smear of endotracheal aspirate yielded gram-negative bacteria, and the isolate grown from endotracheal aspirate culture was identified as S. putrefaciens by Biomerieux API 20 NE technique. …”
Get full text

Morphology and Immunoexpression of Selenoproteins in Term Placenta of Alpaca (<i>Vicugna pacos</i>) from the Peruvian Andes by Miluska Beatriz Navarrete Zamora, Francisco Acuña, Mônica Duarte da Silva, Thamires Santos-Silva, Matheus Henrique Herminio Garcia, Rodrigo da Silva Nunes Barreto, Alberto Sato Sato, Claudio Gustavo Barbeito, Maria Angelica Miglino

“…Placentas were paraffin-embedded, sectioned (3–5 µm), stained with hematoxylin–eosin (H&E), Masson’s trichrome, and picrosirius red, and analyzed via light and polarized light microscopy. …”
Get full text

Extracellular AMP Inhibits Pollen Tube Growth in <i>Picea meyeri</i> via Disrupted Calcium Gradient and Disorganized Microfilaments by Junhui Zhou, Haobo Yang, Yue Zhang, Yuan Cao, Yanping Jing

“…Through intracellular staining for Ca²⁺ and microfilaments, we discovered that high concentrations of AMP disrupt the Ca²⁺ concentration gradient and impair microfilament organization, ultimately resulting in inhibited pollen tube elongation. …”
Get full text

Ocular Surface Disorders in Intensive Care Unit Patients by Tuba Berra Saritas, Banu Bozkurt, Baris Simsek, Zeynep Cakmak, Mehmet Ozdemir, Alper Yosunkaya

“…Conjunctiva hyperemia, mucopurulent or purulent secretion, corneal staining, and corneal filaments were observed in 56.25%, 36.25%, 15%, and 5% of the eyes, respectively. …”
Get full text

Sex-specific effects of prenatal bisphenol A exposure on transcriptome-interactome profiles of autism candidate genes in neural stem cells from offspring hippocampus by Kasidit Kasitipradit, Surangrat Thongkorn, Songphon Kanlayaprasit, Thanit Saeliw, Pattanachat Lertpeerapan, Pawinee Panjabud, Depicha Jindatip, Valerie W. Hu, Takako Kikkawa, Noriko Osumi, Tewarit Sarachana

“…In addition, in vitro proliferation assays were conducted, utilizing immunofluorescence staining for Sox2, a stem cell marker, and BrdU to quantify proliferating NSCs. …”
Get full text

The Increased Expression of HLA-DR and ICAM-1 Molecules by Human Bronchial Epithelial Cells, Induced by Activated Mononuclear Cells, is Downregulated by Nedocromil Sodium by O. Sacco, S. Lantero, L. Scarso, V. Frangova, V. Ottolini, G. A. Rossi

“…The presence of HLA-DR and ICAM-1 molecules on BECs was then evaluated by specific antibody staining and flow-cytometry analysis. The addition to BEC cultures of different concentrations of PHA-stimulated BMC-CM, or of IFN-γ induced a dosedependent increase of HIA-DR and ICAM-1 expression, while no effect was observed with unstimulated BMC-CM. …”
Get full text

Adrenomedullin 2 attenuates anxiety-like behaviors by increasing IGF-II in amygdala and re-establishing blood–brain barrier by Denian Wang, Zhi Yang, Pengfei Wu, Qingyan Li, Chunyan Yu, Ying Yang, Yuefan Du, Mengwei Jiang, Junpeng Ma

“…Furthermore, as evidenced by increased Evans blue staining and decreased VE-cadherin levels, the BBB exhibited dysfunction in ADM2 knockout mice with anxiety-like behaviors. …”
Get full text

Unusual Presentation of Recurrent Early Stage Endometrial Carcinoma 28 Years after Primary Surgery by Alessandro Franchello, Gianruggero Fronda, Giacomo Deiro, Alessia Fiore, Davide Cassine, Luca Molinaro, Luigi Chiusa, Sara Galati, Andrea Resegotti, Stefano Silvestri

“…It is important to keep in mind this possibility in order to set a correct diagnostic and therapeutic algorithm, including preoperative immunohistochemical staining, and to plan a prolonged follow-up program.…”
Get full text

Mast Cells and Histamine: Do They Influence Placental Vascular Network and Development in Preeclampsia? by Grzegorz Szewczyk, Michał Pyzlak, Jakub Klimkiewicz, Wacław Śmiertka, Magdalena Miedzińska-Maciejewska, Dariusz Szukiewicz

“…The concentration of histamine was measured, and immunohistochemical staining for mast cell tryptase was performed. Morphometric analysis was then performed. …”
Get full text

A Picrorhiza kurroa Derivative, Picroliv, Attenuates the Development of Dextran-Sulfate-Sodium-Induced Colitis in Mice by De-Kui Zhang, Jian-Jie Yu, Yu-Min Li, Li-Na Wei, Yi Yu, Yan-Hu Feng, Xiang Wang

“…The expression of NF-κB p65 was observed by immunohistochemistry staining and western blotting. Results. A significant improvement was observed in DAI and histological score in mice treated with picroliv, and incerased MPO activity, MDA concentrations, and the expression of IL-1β, TNF-α, and NF-κB p65 in mice with DSS-induced colitis were significantly reduced while decreased SOD level increased following administration of picroliv. …”
Get full text

Maternal Sevoflurane Exposure Causes Abnormal Development of Fetal Prefrontal Cortex and Induces Cognitive Dysfunction in Offspring by Ruixue Song, Xiaomin Ling, Mengyuan Peng, Zhanggang Xue, Jing Cang, Fang Fang

“…Brain tissues of fetal mice were subjected to immunofluorescence staining to assess differentiation, proliferation, and cell cycle dynamics of the fetal PFC. …”
Get full text

Validation of hyperthermia as an enhancer of chemotherapeutic efficacy: insights from a bladder cancer organoid model by Ying Xu, Guoliang Sun, Tiantian Yang, Huaibiao Li, Poyi Hu, Wanru Luo, Tingke Zhang, Haoran Liu, Guoyi Chen, Zhangqun Ye, Yuqing Wu, Jie Yu, Wanyi Chen, Kai Zhao, Chunyan Liu, Huiping Zhang

“…Proliferation was determined by Ki67 immunofluorescence staining, and apoptosis was assessed using a TdT-mediated dUTP nick end labeling (TUNEL) assay. …”
Get full text

Intraocular Lens Opacification following Intracameral Injection of Recombinant Tissue Plasminogen Activator to Treat Inflammatory Membranes after Cataract Surgery by Simon S. M. Fung, Evripidis Sykakis, Niaz M. Islam, Hadi J. Zambarakji, Ramin Khoramnia, Gerd U. Auffarth, Dipak N. Parmar

“…Light microscopy evaluation revealed diffuse fine granular deposits on the anterior surface/subsurface of IOL optic that stained positive for calcium salts. Scanning electron microscopy (SEM) and energy-dispersive X-ray spectrometry (EDS) confirmed the presence of calcium and phosphate on the IOL. …”
Get full text

IL-6 is Upregulated in Late-Stage Disease in Monkeys Experimentally Infected with Trypanosoma brucei rhodesiense by Dawn Nyawira Maranga, John Maina Kagira, Christopher Kariuki Kinyanjui, Simon Muturi Karanja, Naomi Wangari Maina, Maina Ngotho

“…Brain immunohistochemical staining revealed an increase in brain glial fibrillary acidic protein expression indicative of reactive astrogliosis in infected animals which were euthanized in late-stage disease. …”
Get full text

A Citrus bergamia Extract Decreases Adipogenesis and Increases Lipolysis by Modulating PPAR Levels in Mesenchymal Stem Cells from Human Adipose Tissue by Debora Lo Furno, Adriana Carol Eleonora Graziano, Rosanna Avola, Rosario Giuffrida, Vincenzo Perciavalle, Francesco Bonina, Giuliana Mannino, Venera Cardile

“…To verify adipogenic differentiation, oil red O staining at 7, 14, 21, and 28 days was performed. Moreover, the expression of peroxisome proliferator-activated receptor gamma (PPAR-γ), adipocytes fatty acid-binding protein (A-FABP), adipose triglyceride lipase (ATGL), hormone-sensitive lipase (HSL), monoglyceride lipase (MGL), 5′-adenosine monophosphate-activated protein kinase (AMPK)α1/2, and pAMPKα1/2 was evaluated by Western blot analysis and the release of glycerol by colorimetric assay. …”
Get full text

Inhibition Effect of Phenyllactic Acid on Foodborne Vibrio vulnificus Biofilms by Yanfang ZHANG, Shuilin LIU, Mengjia GUO, Mengmin LÜ, Xinran LÜ, Fengling BAI, Jianrong LI, Fangchao CUI, Xiqian TAN, Hao DONG

“…Then the inhibitory effect of PLA on the formation mass and metabolic activity of V. vulnificus biofilm was analyzed using crystal violet staining method and XTT reduction assay. Using extracellular polysaccharides, extracellular proteins, and extracellular DNA as indicators, the effect of PLA on the extracellular polymers of V. vulnificus was evaluated. …”
Get full text

Handle Region Peptide Ameliorating Insulin Resistance but Not β Cell Functions in Male Rats Neonatally Treated with Sodium L-Glutamate by Guo-shu Yin, Shao-da Lin, Dong-chuan Xu, Ru-qiong Sun, Kun Lin, Chu-jia Lin

“…On the other hand, losartan but not HRP decreased α-cell mass and number of PCNA-positive cells located periphery of the islets and decreased picrosirius red stained area in islets. HRP ameliorating insulin resistance but not β-cell functions leads to hyperglycemia in the end in male MSG rats, and the dual characters of HRP may partly account for the phenomenon.…”
Get full text

An Evaluation of the Biocompatibility and Chemical Properties of Two Bioceramic Root Canal Sealers in a Sealer Extrusion Model of Rat Molars by Shintaro Takahara, Naoki Edanami, Razi Saifullah Ibn Belal, Kunihiko Yoshiba, Shoji Takenaka, Naoto Ohkura, Nagako Yoshiba, Susan Gomez-Kasimoto, Yuichiro Noiri

“…After 28 days, periapical tissue reactions were assessed using microcomputed tomography and histological staining. The elemental composition and chemical composition of the extruded EBC and NBG were analyzed at Day 1 and 28 using an electron probe microanalyzer and micro-Raman spectroscopy. …”
Get full text