Deciphering the significance of neutrophil to lymphocyte and monocyte to lymphocyte ratios in tuberculosis: A case-control study from southern India [version 2; peer review: 2 appr... by Poorva Bakshi, Shikha Jayasheelan, Rakshatha Nayak, Sharada Rai

“…Background Diagnosis of tuberculosis (TB) in resource-limited countries relies primarily on bacteriological confirmation using Ziehl-Neelsen (ZN) stain on sputum or other representative samples. …”
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A case report of hermaphrodite goat: clinical and genetic approach by Çağlayan Özel, Gonca Şen, Mustafa Hitit, Nadir Koçak, Aydın Güzeloğlu, Ahmet Semacan, Ercan Kurar, Ercan

“…After chromosome preparation using peripheral blood samples and GTG staining, appropriate metaphases were analyzed using an image analyzer program. …”
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Integrated single-cell RNA sequencing reveals the tumor heterogeneity and microenvironment landscape during liver metastasis in adenocarcinoma of esophagogastric junction by Junrui Xu, Ussama Sadiq, Wangruizhi Zhao, Hengbo Xia, Yiwei Liu, Renquan Zhang, Aman Xu

“…The incorporation of GEO, TCGA, and immunofluorescence staining of SFRP2 expression enhanced our study. High expression of SFRP2 in pericytes may influence vascular stability and angiogenesis through the Wnt pathway.ConclusionOur study provides novel insights into the cellular interactions and molecular mechanisms that underlie AEGJ liver metastasis. …”
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Rat Nasal Respiratory Mucosa-Derived Ectomesenchymal Stem Cells Differentiate into Schwann-Like Cells Promoting the Differentiation of PC12 Cells and Forming Myelin In Vitro by Jian Zhang, Xin Gao, Hongjun Zou, Jinbo Liu, Zhijian Zhang

“…The differentiated REMSCs (dREMSCs) exhibited a spindle-like morphology similar to SC cells. Immunocytochemical staining and Western blotting indicated that SC-like cells expressed the glial markers (GFAP, S100β, Galc, and P75) and CNPase. …”
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Autologous precision-cut lung slice co-culture models for studying macrophage-driven fibrosis by So-Yi Chang, So-Yi Chang, Wen-Hsin Chang, Wen-Hsin Chang, David C. Yang, Qi-Sheng Hong, Qi-Sheng Hong, Ssu-Wei Hsu, Ssu-Wei Hsu, Reen Wu, Ching-Hsien Chen, Ching-Hsien Chen

“…Fibrotic responses to nicotine, cigarette smoke extract (CSE), and a fibrosis-inducing cocktail (FC) were first evaluated in PCLS containing only tissue-resident macrophages, with upregulation of α-SMA-expressing fibroblasts confirmed by immunofluorescence and Western blotting, and collagen deposition quantified using Sirius Red staining. To study macrophage recruitment, we employed an indirect co-culture model using transwells to approximate blood vessel function. …”
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Lynch syndrome screening in colorectal and endometrial cancers in Iceland by Katla R. Kluvers, Thordur Tryggvason, Vigdis Stefansdottir, Jon G. Jonasson, Petur Snaebjornsson, Sigurdis Haraldsdottir

“…Results: In 2020–2022, 429 of 522 (82%) diagnosed CRCs were stained and 90 of 106 (85%) ECs, compared to 74% of CRCs and 82% of ECs in 2017–2019. …”
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Textural Analysis of Nuclear Mitotic Apparatus Antigen (NuMA) Spatial Distribution in Interphase Nuclei from Human Drug-Resistant CEM Lymphoblasts by Naima Rafki‐Beljebbar, Françoise Liautaud‐Roger, Dominique Ploton, Jean Dufer

“…Therefore, we have compared the textural features observed in G0/G1 nuclei from human leukemic CEM cells and their MDR variant CEM‐VLB, after staining of either DNA by Feulgen method or nuclear matrix by immunodetection of NuMA antigen on DNase treated samples. …”
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EXO1 is a key gene for lung-resident memory T cells and has diagnostic and predictive values for lung adenocarcinoma by Zhuoqi Li, Xiaoyan Lin, Yuanhui Yang, Mei Tian, Lu Zhang, Fujing Huang, Xiao Wen, Zhigang Wei, Yuan Tian

“…The results were validated through immunohistochemical staining of EXO1 in LUAD samples. The results showed EXO1 was aberrantly highly expressed in LUAD tissues. …”
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Toxic Effects of Ethyl Cinnamate on the Photosynthesis and Physiological Characteristics of Chlorella vulgaris Based on Chlorophyll Fluorescence and Flow Cytometry Analysis by Yang Jiao, Hui-Ling Ouyang, Yu-Jiao Jiang, Xiang-Zhen Kong, Wei He, Wen-Xiu Liu, Bin Yang, Fu-Liu Xu

“…Parameters, including biomass, Fv/Fm (maximal photochemical efficiency of PSII), ФPSII (actual photochemical efficiency of PSII in the light), FDA, and PI staining fluorescence, were measured. The results showed the following: (1) The inhibition on biomass increased as the exposure concentration increased. 1 mg/L ethyl cinnamate was sufficient to reduce the total biomass of C. vulgaris. …”
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Effect of Stretching Force on the Cells of Epithelial Rests of Malassez In Vitro by Teruyoshi Koshihara, Kenichi Matsuzaka, Toru Sato, Takashi Inoue

“…The cells were observed by immunohistochmical staining using actin 0, 12, 24, 36, 48, and 72 hours. …”
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Rapid Multi-Well Evaluation of Assorted Materials for Hydrogel-Assisted Giant Unilamellar Vesicle Production: Empowering Bottom-Up Synthetic Biology by Cherng-Wen Darren Tan, Magdalena Schöller, Eva-Kathrin Ehmoser

“…PLE-GUVs offer a novel method of GUV labelling and tracing, which emulates the calcein-AM staining behaviour of cells. Our results highlight the utility of our protocol for potentiating substrate material discovery, as well as protocol and product development.…”
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Microbial Contamination in Chicken Meat in Traditional Markets in Banyuwangi by Prima Ayu Wibawati, Yunia Chrismonica, Racca Biana Susanti, Mario Putra Himawan, Afaf Heny Sintya Devy, Juwita Ayu Nirmalasari, Abzal Abdramanov

“…E. coli and Salmonella spp. were identified using Gram staining, culturing, and biochemical tests. The Most Probable Number (MPN) method was used for coliform and E. coli. …”
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Pathogenic Role of Iron Deposition in Reticuloendothelial Cells during the Development of Chronic Hepatitis C by Hironori Mitsuyoshi, Kohichiroh Yasui, Kanji Yamaguchi, Masahito Minami, Takeshi Okanoue, Yoshito Itoh

“…Serum transaminase levels and hepatic scores of stage, grade, and steatosis were higher in patients with REC iron staining than in those without. REC iron scores were independently associated with advanced stage. …”
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Protective effect of adiponectin on oxidative stress-induced ovarian granulosa cell senescence in geese by Yan Zheng, Yunqiao Qiu, Ming Gao, Qianhui Wang, Lei Yu, Zhongzan Cao, Xinhong Luan

“…To investigate this, senescence in primary goose GCs was induced by D-gal and assessed via RT‒qPCR, senescence-associated β-galactosidase (SA-β-gal) staining, immunofluorescence, flow cytometry, and transcriptomics. …”
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Extramedullary Hematopoiesis in Uterine Leiomyoma Associated with Numerous Intravascular Thrombi by Xiaoyan Cui, Deniz Peker, Heather O. Greer, Michael G. Conner, Lea Novak

“…Microscopic examination revealed benign smooth muscle consistent with leiomyoma and numerous intravascular thrombi both with areas of EMH. Immunohistochemical stains confirmed the presence of all three benign lineages of hematopoietic cells. …”
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TACSTD2 in gelatinous drop-like corneal dystrophy: variant functional analysis and expression in the cornea after limbal stem cell transplantation by Liubov O. Skorodumova, Ekaterina N. Grafskaia, Daria D. Kharlampieva, Dmitry I. Maltsev, Tatiana V. Petrova, Alexandra V. Kanygina, Elena V. Fedoseeva, Pavel V. Makarov, Boris E. Malyugin

“…Taken together, the results of transcriptome analysis and immunofluorescence staining of post-LSCT corneal sample with amyloid deposits obtained during keratoplasty demonstrated complete restoration of wild-type TACSTD2 expression, indicating that donor CECs replaced host CECs. …”
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The SGLT-2 Inhibitor Dapagliflozin Has a Therapeutic Effect on Atherosclerosis in Diabetic ApoE−/− Mice by Weiling Leng, Xinshou Ouyang, Xiaotian Lei, Mingxia Wu, Liu Chen, Qinan Wu, Wuquan Deng, Ziwen Liang

“…The atherosclerosis was evaluated by oil red O and hematoxylin-eosin staining. The effects of dapagliflozin on the IL-1β production in culturing primary macrophages of wild type and NLRP3−/− knockout mice were investigated for mechanism analyses. …”
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Genome-wide and rna-seq highlight genetic characteristics of rumpless signals in piao chicken by Wang Mei QI, Xing Fu ZHANG, Li Wen SONG, Zai Xia LIU, Yuan CHAI, Yan Yong SUN

“…In this study, transparent staining was performed on the bones of 16 tailed chickens and 16 Piao chickens to observe the dynamic bone formation process of embryos at different developmental stages. …”
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In Vivo and Impression Cytology Study on the Effect of Compatible Solutes Eye Drops on the Ocular Surface Epithelial Cell Quality in Dry Eye Patients by Manuela Lanzini, Claudia Curcio, Rossella Annamaria Colabelli-Gisoldi, Alessandra Mastropasqua, Roberta Calienno, Luca Agnifili, Mario Nubile, Leonardo Mastropasqua

“…No significant difference in Schirmer I Test, TBUT, and vital staining results was observed during the follow-up period in both groups. …”
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Technogenic effects on scots pine generative organs: a cross-country study by Gulmira Assylbekova, Bibigul Zhumabekova, Mikhail Klimenko, Mulk Khan Shujaul, Karlygash Aubakirova

“…For cytological analysis, microsporophylls were selected from the middle part of the microstrobilus, the material was stained with 1% Lugol's pharmaceutical solution, the preparations were studied on a Nikon eclipse E 200 microscope with a magnification of 10x15x40. …”
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