Acute Genetic Damage Induced by Ethanol and Corticosterone Seems to Modulate Hippocampal Astrocyte Signaling by Ana Laura Reyes-Ábalos, Magdalena Álvarez-Zabaleta, Silvia Olivera-Bravo, María Vittoria Di Tomaso

“…Genetic damage and initial DDR were assessed by immunolabeling against the phosphorylated histone variant H2AX (γH2AX), DDR-dependent apoptosis by BAX immunoreactivity, and astrocyte activation by the glial acidic fibrillary protein (GFAP) and phalloidin staining. Surface-attached EVs and NTs were examined via scanning electron microscopy, and labeled proteins were analyzed via confocal microscopy. …”
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In vitro antitumor effects of methanolic extracts of three Ganoderema mushrooms by Elshahat A. Toson, Amira A. El-Fallal, Marwa A. Oransa, Hoda M. El-Gharabawy

“…In this regard, Sulfo-rhodamine B staining and flow cytometry were used. Hepatocellular carcinoma (HepG2) and breast ductal carcinoma (T-47D) cell lines were used as cancer models, while mouse normal liver (BNL) and oral epithelial cell (OEC) lines were used as respective controls. …”
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Gastrointestinal Parasites With Their Risk Factors in Tharu Indigenous People in Southern Nepal: A Cross‐Sectional Study by Pinki Kumari Chaudhary, Tirth Raj Ghimire

“…The samples were analyzed by direct wet mount and acid‐fast staining techniques and examined at 40×, 100×, 400×, and 1000× magnifications. …”
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Primary Mechanism Study of Panax notoginseng Flower (Herb) on Myocardial Infarction in Rats by Xin Zhou, Zhi-Cheng Li, Pei-Pei Chen, Rui-Fang Xie

“…HE (hematoxylin and eosin) staining, immunofluorescent assay, TUNEL assay, quantitative real-time PCR, and western blot analysis were, respectively, performed to observe morphology, count mean minimal vessels, investigate apoptotic cells, and record gene (HIF-1, VEGFA, and KDR) and protein (Bcl-2 and Bax) expressions. …”
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Activation of endogenous tolerance to bleaching stress by high salinity in cloned endosymbiotic dinoflagellates from corals by Ching-Nen Nathan Chen, Tze Ching Yong, Jih-Terng Wang

“…Pretreatment at 50 ppt salinity reduced the percentage of cells stained for ROS by 59% and 64% in the two species under bleaching stress compared to those incubated at 30 ppt. …”
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Genistein Protects against Spinal Cord Injury in Mice by Inhibiting Neuroinflammation via TLR4-Mediated Microglial Polarization by Xin-Wu Li, Peng Wu, Jian Yao, Kai Zhang, Gen-Yang Jin

“…Apoptosis was determined by TUNEL staining, and inflammation was evaluated by measuring inflammatory factors by an ELISA kit. …”
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The Efficacy of Fecal Microbiota Transplantation in Experimental Autoimmune Encephalomyelitis: Transcriptome and Gut Microbiota Profiling by Sanwang Wang, Hongliang Chen, Xin Wen, Jingjing Mu, Mingyue Sun, Xiaowen Song, Bin Liu, Jinbo Chen, Xueli Fan

“…We measured the severity of disease by clinical EAE scores and H&E staining. Gut microbiota alteration in the gut and differentially expressed genes (DEGs) in the spinal cord were analyzed through 16S rRNA and transcriptome sequencing. …”
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Analysis of the Renal Protection and Antioxidative Stress Effects of Panax notoginseng Saponins in Diabetic Nephropathy Mice by Wei Mi, Min Yu, Shuying Yin, Yuhan Ji, Tala Shi, Ning Li

“…Eight weeks after intervention, the 24 h urine protein (UPro) and urinary albumin (UAlb) were quantitatively examined, and the related blood biochemical indices were measured. HE and PAS staining were performed for pathological changes of renal tissue. …”
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Incorporation of recombinant proteins into extracellular vesicles by Lactococcus cremoris by Tina Vida Plavec, Kristina Žagar Soderžnik, Giulia Della Pelle, Špela Zupančič, Robert Vidmar, Aleš Berlec

“…The presence of vesicular structures (50–200 nm) in the samples was confirmed by transmission electron microscopy and by flow cytometry using membrane-specific stain. Mass spectrometry analyses confirmed the presence of recombinant proteins in the EVs fraction, with amounts ranging from 13.17 to 100%, highlighting their significant incorporation into the vesicles, together with intrinsic L. cremoris NZ9000 proteins that were either more abundant in the cytoplasm (ribosomal proteins, metabolic enzymes) or present in the membrane. …”
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The Roles of Autophagy, Mitophagy, and the Akt/mTOR Pathway in the Pathogenesis of Chronic Rhinosinusitis with Nasal Polyps by Chen Wang, Min-Li Zhou, Yong-Cai Liu, Ke-Jia Cheng

“…Masson’s trichrome and periodic acid–Schiff Alcian blue staining were used to evaluate the severity of tissue remodeling. …”
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Frequency and Distribution of Parasites Detected in Children Between 2017-2022 (6-year Period) by Yelda Sorguç

“…The stool samples were examined with saline-Lugol, modified Kinyoun acid-fast and trichrome staining methods. Results: Parasites were detected in the stool samples of 1.8% of a total of 36,748 patients. …”
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The Long Noncoding RNA Gm9866/Nuclear Factor-κB Axis Promotes Macrophage Polarization by Xiaomin Liao, Xianxian Ruan, Xiubing Chen, Biaolin Zheng, Xianbin Wu, Zhejun Deng, Zhe Yang, Feng Wang, Shanyu Qin, Haixing Jiang

“…EdU (5-ethynyl-2′-deoxyuridine) and TUNEL (TdT-mediated dUTP nick-end labeling) staining assays revealed that overexpression of long noncoding RNA Gm9866 promoted cell proliferation and inhibited apoptosis. …”
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Dimethyl Fumarate Protects against Lipopolysaccharide- (LPS-) Induced Sepsis through Inhibition of NF-κB Pathway in Mice by He Fang, Xingtong Wang, Mahendra Damarla, Rongju Sun, Qingli He, Ruojing Li, Pengfei Luo, Jun O. Liu, Zhaofan Xia

“…The survival rate was assessed, and HE staining was performed to investigate histopathological damage to the organs. …”
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Constructing an Isogenic 3D Human Nephrogenic Progenitor Cell Model Composed of Endothelial, Mesenchymal, and SIX2-Positive Renal Progenitor Cells by Lisa Nguyen, Lucas-Sebastian Spitzhorn, James Adjaye

“…Immunofluorescence-based stainings of sectioned 3D-NPCs revealed cells expressing the renal progenitor cell markers (SIX2 and PAX8), podocyte markers (Nephrin and Podocin), the endothelial marker (CD31), and mesenchymal markers (Vimentin and PDGFR-β). …”
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miR-486-5p Restrains Extracellular Matrix Production and Oxidative Damage in Human Trabecular Meshwork Cells by Targeting TGF-β/SMAD2 Pathway by Le Xu, Yiming Zhang, Hua Long, Bo Zhou, Haibo Jiang

“…TMCs were disposed with 300 μM H2O2 to establish oxidative damage models in vitro. miR-486-5p mimics and its controls were transfected into TMCs, and cell apoptosis and extracellular matrix production (ECM) genes were measured by flow cytometry, western blotting, and immunofluorescence staining. Activities of superoxide dismutase (SOD) and malondialdehyde (MDA) were also assayed. …”
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Cystatin C Secretion in Blood Derivatives and Cellular Models of Idiopathic Parkinson’s Disease by Kathleen Mommaerts, Anna S. Monzel, Alise Zagare, Sarah L. Nickels, Nico J. Diederich, Laura Longhino, William Mathieson, Paul M. A. Antony, Fay Betsou, Jens C. Schwamborn

“…The identity of the derived models was confirmed using immunohistochemical staining. Secreted cystatin C concentration was measured in each biospecimen tested. …”
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Magnetic Resonance Imaging of Contrast-Induced Acute Renal Injury and Related Pathological Alterations In Vivo by Yanfei Li, Dafa Shi, Haoran Zhang, Xiang Yao, Ke Ren

“…Magnetic resonance imaging of 9.4 T scanner and microscopic appearance of renal H&E staining were tools to test the occurrence of CI-AKI at different times. …”
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Diagnosis and Prognosis of Neuroendocrine Tumours of the Lung by Means of High Resolution Image Analysis by Uta Jütting, Peter Gais, Karsten Rodenacker, Joachim Böhm, Susanne Koch, Heinz W. Präuer, Heinz Höfler

“…Sections 4 μm thick from paraffin embedded tissue were Feulgen stained. By means of high resolution image analysis 100 nuclei per case were randomly gathered to extract morphometric, densitometric and textural quantitative features. …”
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Oral Hyaluronic Acid Supplementation for the Treatment of Dry Eye Disease: A Pilot Study by Yeseul Kim, Chan Hee Moon, Bo-Yeon Kim, Sun Young Jang

“…The inclusion criteria were as follows: (1) >18 years of age; (2) distance best-corrected visual acuity ≥ 20/40 Snellen equivalent in each eye; (3) IOP ≤ 21 mmHg in both eyes; (4) ocular surface disease index (OSDI) score of ≥18 and <65; (5) <10 seconds of tear break up time (TBUT); (6) >5 corneal spots of corneal fluorescein staining (CFS); and (7) ≤ 10 mm/5 min of the Schirmer test. …”
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Status of the Multidrug Resistance-1 Gene of Plasmodium falciparum in Four Malaria Epidemiological Strata, Two Decades after the Abolition of Chloroquine as First-Line Treatment fo... by David Denis Sofeu-Feugaing, Fabrice Nkengeh Ajonglefac, Marcel Nyuylam Moyeh, Tobias Obejum Apinjoh, Marianne Elodie Essende, Gilchrist Destin Talla Kouam, Stephen Mbigha Ghogomu

“…Fragments were analyzed using a 3% ethidium bromide-stained agarose gel. P. falciparum was the most abundant Plasmodium species, accounting for 87.21% of P. falciparum monoinfections only. …”
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