Expression Levels of IL-17A, IL-17F, IL-17RA, and IL-17RC in Prostate Cancer with Taking into Account the Histological Grade according to Gleason Scale in Comparison to Benign Pros... by Marlena Janiczek, Łukasz Szylberg, Paulina Antosik, Anna Kasperska, Andrzej Marszałek

“…Expression of the antibody to be examined using light microscopy and the Remmele-Stegner score (IRS) in cancer staining was then evaluated. Expression of IL-17 RA was not shown in a group of patients with PCa and in the control group. …”
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Discussion on the Mechanism of Gandoufumu Decoction Attenuates Liver Damage of Wilson’s Disease by Inhibiting Autophagy through the PI3K/Akt/mTOR Pathway Based on Network Pharmacol... by Lulu Tang, Chenling Zhao, Jing Zhang, Ting Dong, Huaizhen Chen, Taohua Wei, Jiuxiang Wang, Wenming Yang

“…Hematoxylin-eosin (HE), Masson, and Sirius red staining were used for the characterization of liver pathological changes. …”
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The Invasion and Metastasis of Colon Adenocarcinoma (COAD) Induced by SALL4 by Wenjuan Zhang, Yan Hu, Wenbing Zhang, Ke Yi, Xiaohui Xu, Zhihua Chen

“…The immunofluorescence staining confirmed the EMT phenotype. On the contrary, knocking out SALL4 gene reversed EMT, weakened cell proliferation and invasion, inhibited upregulation of Twist, vimentin, and N-cadherin expressions and downregulation of E-cadherin. …”
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Characterization of Transverse Aortic Constriction in Mice Based on the Specific Recruitment of Leukocytes to the Hypertrophic Myocardium and the Aorta Ascendens by Jan Lukas Kleiner, Odilia Köpke, Anton Faron, Yunyang Zhang, Jan Cornelssen, Mark Coburn, Stilla Frede, Lars Eichhorn, Christina Katharina Weisheit

“…We used complex flow cytometry staining combinations to characterize the various innate immune cell subsets and a multiplex array to determine cytokine concentrations in the serum. …”
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Umbilical Cord Mesenchymal Stem Cells Overexpressing Heme Oxygenase-1 Promotes Symptoms Recovery in Cystitis Rats by Alleviating Neuroinflammation by Qiongqiong Gao, Zhentao Gao, Minzhi Su, Yong Huang, Chi Zhang, Cuiping Li, Hailun Zhan, Bolong Liu, Xiangfu Zhou

“…Additionally, western blot, immunofluorescence staining, and ELISA kit test for anti-inflammation effect. …”
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Intraoperative Complications of Cataract Surgery Using Intracameral Illumination in the Elderly over 75 Years by Yu Jeong Kim, Su Jin Park, Jong Yeon Lee, Dae Yeong Lee, Dong Heun Nam

“…Intraoperative complications (posterior capsule rupture, radial tear of the anterior capsule, dropped nucleus, or sulcus-implanted/sclera-fixated IOL) and utilization of adjunctive devices (pupil expansion device or anterior capsule staining). Results. The frequency of use of the pupil expansion device was lower in the advanced cataract surgery group than that in the standard cataract surgery group (0.7% vs 6.9%; p=0.007). …”
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Acupuncture Alleviates CUMS-Induced Depression-Like Behaviors by Restoring Prefrontal Cortex Neuroplasticity by Peng Li, Wenya Huang, Yiping Chen, Muhammad Shahzad Aslam, Wenjing Cheng, Yang Huang, Wenjie Chen, Yanxun Huang, Xinnan Wu, Yining Yan, Junliang Shen, Tao Tong, Shuqiong Huang, Xianjun Meng

“…The number of nerve cells, dendrites’ length, and the prefrontal cortex’s spine density were detected using Golgi staining. The prefrontal cortex expression, such as BDNF, PSD95, SYN, and PKMZ protein, was detected using the western blot and RT-PCR. …”
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Bovine Trypanosomosis and Its Vectors in Three Selected Districts of Buno Bedele Zone of Oromia Region, Ethiopia by Gelaye Gebisa, Kibiru Beriso, Biruk Bogale, Oda Gizaw, Dawit Chala

“…The buffy coat technique was used to check the presence of parasites from sampled blood, and the trypanosome species were identified using Giemsa-stained thin blood films. The packed cell volume of sampled blood was determined using the haematocrit. …”
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Renewable Human Cell Model for Type 1 Diabetes Research: EndoC-βH5/HUVEC Coculture Spheroids by James M. Porter, Michael Yitayew, Maryam Tabrizian

“…Insulin secretion, metabolic activity, live cell fluorescence staining, and gene expression assays were used to compare the viability and functionality of spheroids composed of 100% beta-cells, 1 : 1 beta-cell/endothelial, and 1 : 3 beta-cell/endothelial. …”
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Proton pump inhibitors enhance chemosensitivity, promote apoptosis, and suppress migration of breast cancer cells by Ihraiz Worood G., Ahram Mamoun, Bardaweel Sanaa K.

“…The mechanism of cell death was assessed using annexin V-FITC/propidium iodide staining flow cytometry method. Our results indicated that PPIs treatment significantly inhibited the growth of breast cancer cells in a dose-dependent manner. …”
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Anticancer Potential of Fruit Extracts from Vatica diospyroides Symington Type SS and Their Effect on Program Cell Death of Cervical Cancer Cell Lines by Atchara Chothiphirat, Kesara Nittayaboon, Kanyanatt Kanokwiroon, Theera Srisawat, Raphatphorn Navakanitworakul

“…Flow cytometry analysis with Annexin V-FITC and PI staining precisely indicated that ACE induced apoptosis in HeLa and SiHa cell lines in a dose-dependent manner. …”
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Exosomes derived from liver failure patients’ plasma stimulated mesenchymal stem cells alleviate acute liver failure by Zhuoran Wang, Jun Ling, Shaoli You, Bing Zhu

“…Liver tissue damage was examined by hematoxylin and eosin staining and immunohistochemistry. The levels of signaling pathway proteins were determined by western blotting. …”
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Screening of Anti-Hair Loss Plant Raw Materials Based on Reverse Network Pharmacology and Experimental Validation by Jiajia Xu, Congfen He, Rui Tian

“…Licorice water extract (LWE), salvia miltiorrhiza water extract (SMWE), mulberry leaf water extract (MLWE), ephedra water extract (EWE) and curcumae radix water extract (CRWE) did not exhibit cytotoxicity on human dermal papilla cells (HDPCs). Through ALP staining, it was found that the expression of ALP in HDPCs treated with LWE, SMWE, MLWE, EWE and CRWE was enhanced. …”
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GOLPH3 inhibits glioma cell apoptosis through the JNK signaling pathway by Shao Xie, Shao Xie, Jiahai Ding, Zhaohao Wang, Hengliang Shi, Zheng-Quan Yu

“…Apoptosis was assessed using flow cytometry, immunofluorescence staining, TUNEL assays, and Western blotting. The activation of the JNK signaling pathway was evaluated by analyzing the phosphorylation levels of JNK and c-Jun through Western blotting.ResultsDownregulation of GOLPH3 in U87 glioma cells significantly enhanced apoptosis, as evidenced by increased levels of cleaved caspase-3 and higher apoptosis rates. …”
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New taphonomic approach applied to the Late Pleistocene bone remains from Pikimachay Cave, Ayacucho Basin, Peru: possible implications for the debate on human colonisation of weste... by Hugo G Nami, Karina V Chichkoyan, Juan Yataco Capcha, José L Lanata

“…For this purpose, we examined the surface modifications indicating fresh or post-depositional fractures, the different kinds of marks, weathering, and the presence of manganese staining. We concluded that several bones (n=8) from layer h showed various human-made modifications. …”
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Nuclear DNA Content and Chromatin Pattern of Rat Rhabdomyosarcoma Cell Sublines with Different Metastatic Potentials by Jean Dufer, Marie-France Poupon, Sonia Yatouji

“…The aim of this work was to characterize nuclear changes observed in metastatic cell clones issued in vitro from the same parental cell population The nuclear phenotypes of 6 cell sublines isolated from a rat rhabdomyosarcoma cell line and differing in their metastatic ability were evaluated by image cytometry on Feulgen‐stained preparations. Densitometric [5], geometric [3] and textural [9] features were computed from each nuclear image. …”
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Reduced Fascicle Area Demonstrated in Ilioinguinal Nerves Resected from Primary Inguinal Herniorrhaphy Patients as Evidence of Compression Neuropathy by Robert Wright, Donald E. Born, Troy Sanders, Jordan Landes, Troy Salisbury, Anjali S. Kumar, Makena Horne

“…Three samples of the resected ilioinguinal nerve (proximal, canal, and distal) were evaluated using Masson’s trichrome stain to measure fascicle and total nerve cross-sectional area and detect changes in collagen. …”
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Trichomonas vaginalis and Associated Factors among Pregnant Women Attending Antenatal Care at Bule Hora University Teaching Hospital, Oromia Region, Southern Ethiopia by Oliyad Husen, Alqeer Aliyo, Kalicha Boru, Tibeso Gemechu, Wako Dedecha, Girma Ashenafi

“…The two vaginal swabs were collected by brushing the vagina with a sterile cotton swab and tested by using direct wet mount and the Giemsa staining. The data were analyzed using SPSS version 26 for logistic regression analysis. …”
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Abdominal Massage Improves the Symptoms of Irritable Bowel Syndrome by Regulating Mast Cells via the Trypase-PAR2-PKCε Pathway in Rats by Huanan Li, Wei Zhang, Fei Ma, Xiaofan Zhang, Yuyan Wang, Jingui Wang

“…On day 14, the rats were euthanized and the tissues were analyzed by transmission electron microscopy (TEM), immunohistochemistry or immunofluorescence staining, and laser confocal focus to visualize the micromorphology of the intestinal mucosa. …”
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Inhibition of miR-652-3p Regulates Lipid Metabolism and Inflammatory Cytokine Secretion of Macrophages to Alleviate Atherosclerosis by Improving TP53 Expression by Haiyun Liu, Changpeng Zuo, Lijuan Cao, Naiquan Yang, Tingbo Jiang

“…After ox-LDL treatment and miR-652-3p mimic, si-TP53 and their controls transfection, ELISA, and Q-PCR assays were used to detect IL-1ß, IL-6, and TNF-α levels. oil red O staining was processed to verify cholesterol accumulation. …”
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