Inflammatory Mediators in Induced Sputum and Airway Hyperresponsiveness in Cough Variant Asthma during Long-Term Inhaled Corticosteroid Treatment by Meixuan Liu, Kaixiong Liu, Ning Zhu, Jingwen Xia, Xiaodong Chen

“…Interleukin- (IL-) 5 and IL-10 were assayed by ELISA, and the percentage of eosinophils was detected with Giemsa stain. Trends during the follow-up period were analyzed using a general linear model. …”
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Cell Surface Proteins for Enrichment and In Vitro Characterization of Human Pluripotent Stem Cell-Derived Myogenic Progenitors by Sin-Ruow Tey, Madison Mueller, Megan Reilly, Colton Switalski, Samantha Robertson, Mariko Sakanaka-Yokoyama, Masatoshi Suzuki

“…The ability of muscle differentiation in the resulting cells was characterized by immunofluorescent labeling and quantification of positively stained cells. Our results revealed that myotube-forming cells resided in the differentiated cultures of CD29+, CD56+, CD271+, and CD15– fractions, while thick and multinucleated myotubes were identified in the differentiated cultures from CD9+ and CD146+ fractions. …”
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Diversity and occurrence of methylotrophic yeasts used in genetic engineering by A. S. Rozanov, E. G. Pershina, N. V. Bogacheva, V. Shlyakhtun, A. A. Sychev, S. E. Peltek

“…Whereas their status  for commercial use is undetermined, the search for alternative stains for expression of recombinant protein continues.  …”
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Polydatin ameliorates early brain injury after subarachnoid hemorrhage through up-regulating SIRT1 to suppress endoplasmic reticulum stress by Yuwei Han, Guangzhi Hao, Song Han, Tingzhun Zhu, Yushu Dong, Ligang Chen, Xinyu Yang, Xiaoming Li, Hai Jin, Guobiao Liang

“…Terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) staining was used to detect cell apoptosis, and transmission electron microscopy was used to observe the ultrastructure of the endoplasmic reticulum.ResultsThe results indicated that PD significantly reduced brain edema and Evans blue extravasation after SAH, improving neurological function. …”
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Bisphenol S Induces Lipid Metabolism Disorders in HepG2 and SK-Hep-1 Cells via Oxidative Stress by Kai-Xing Lin, Zi-Yao Wu, Mei-Lin Qin, Huai-Cai Zeng

“…Increased lipid droplets were observed using morphological Oil Red O staining. Our study demonstrates that BPS may cause lipid accumulation by increasing oxidative stress and perturbing cellular lipid metabolism.…”
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The Anti-proliferative Effect of a Novel Glutaminase Inhibitor IN-3 on Prostate Cancer Cells by Ummuhan DEMIR, Ayse Busranur CELIK

“…Methods: The cell viability upon IN-3 treatment was examined using crystal violet staining and IC50 values were calculated for cancer cell lines PC-3 and LNCaP and normal fibroblasts CCD1072sk. …”
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Fungal Keratitis Caused by Drechslera spp. Treated with Voriconazole: A Case Report by Margarita I. Echavez, Archimedes Lee D. Agahan, Noel S. Carino

“…Corneal scraping of the ulcer showed no organisms on Gram and Giemsa stain. Cultures were positive for Drechslera spp. and patient was started on Natamycin drops every 15 minutes, Atropine drops 3× a day, and Levofloxacin was continued every 4 hours. …”
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Environmental quality and macroeconomic phenomenon by S. Amar, A. Satrianto, A. Ariusni, A. Ikhsan, E.S. Siregar

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Pathological effects of pregabalin toxicity in rats by Roaa Salih Mahdi, Nawras Najah, Sura Salman Ejam

“…Results: Focal regions of hemorrhage and congestion were seen in H&E-stained sections from the acute toxicity group, and most pyramidal cells were degraded, pyknotic, and exhibited karyolysis. …”
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Effect of Black Cumin on the Expression of Genes Related to Lipid Metabolism in Adipose Tissue: A Mouse Model Study by Roya Maleki, Ali Ahadi, Hoda Ayat

“…In addition, adipose tissue and cell size were analyzed using the Oil Red O staining method and quantitation using Image J software. …”
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Value of alpha smooth muscle actin (αSMA) and inducible nitric oxide synthase (iNOS) in oral squamous cell carcinoma (OSCC): An immunohistochemical approach to tumor stroma categor... by Heba E.M. Youssef, Basant Hamdy AbouZaid

“…Methodology: Thirty-two archival specimens of OSCC were stained immunohistochemically with anti-α-SMA and anti-iNOS. …”
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Therapeutic Potential of Liraglutide for Diabetes–Periodontitis Comorbidity: Killing Two Birds with One Stone by Man Yang, Yunqing Pang, Minyu Pei, Yuanyuan Li, Xuemin Yuan, Rongbing Tang, Jing Wang

“…The morphological changes of periodontal tissues were observed by hematoxylin–eosin staining. The absorption of alveolar bone and its ultrastructural changes were observed by histomorphometry and microcomputed tomography. …”
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Clinical, histopathological and immunohistochemical analysis of vulvar squamous cell carcinoma by Lorenza Bridi Todeschini, Rita de Cássia Sant’Anna Alves, Adriana Vial Roehe

“…Positive p16 and PDL1 staining was detected in 24.4% and 17.1% of the samples, respectively. p53 expression was negative in 19.5% of the samples, whereas it was overexpressed in 24.4%. p16-positive tumors showed a smaller depth of invasion (DOI) (p = 0.014), while tumors with p53 abnormal expression showed greater DOI (p = 0.041). …”
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Plexin C1 Marks Liver Cancer Cells with Epithelial Phenotype and Is Overexpressed in Hepatocellular Carcinoma by Gorkem Odabas, Metin Cetin, Serdar Turhal, Huseyin Baloglu, A. Emre Sayan, Tamer Yagci

“…In silico and in vitro analyses revealed a Plexin C1-based clustering of well-differentiated HCC cell lines. Staining of HCC and nontumoral liver tissues with PE4 showed a membrane-localized overexpression of Plexin C1 in tumors (p=0.0118). …”
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Neuroprotective Effect of Wharton’s Jelly-Derived Mesenchymal Stem Cell-Conditioned Medium (WJMSC-CM) on Diabetes-Associated Cognitive Impairment by Improving Oxidative Stress, Neu... by Zohre Aghaei, Narges Karbalaei, Mohammad Reza Namavar, Masoud Haghani, Mahboobeh Razmkhah, Mahdi Khorsand Ghaffari, Marzieh Nemati

“…The histopathological evaluation of the hippocampus was performed by Nissl staining. The expression levels of Bax, Bcl-2, BDNF, and TNF-α were detected by real-time polymerase chain reaction (RT-PCR). …”
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ABA enhances the apoptotic effect of docetaxel in the multidrug-resistant DU145 prostate cancer cell line by Şumnulu Deniz, Doğanlar Zeynep Banu

“…Tali™ cytometry analysis showed a 47% increase in apoptotic/necrotic cells with the combined treatment of docetaxel and ABA, compared to a 26% increase with docetaxel alone. Fluorescent staining revealed that co-administration of docetaxel and ABA increases apoptosis in resistant DU145 cells compared to treatment with docetaxel alone. …”
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Serum hepcidin level with iron profile assay might replace bone marrow iron study as a diagnostic tool for evaluation of anemia in elderly without chronic renal disease by Sukdev Manna, Sankha Shubhra Chakrabarti, Rohit Singh, Indrajeet Singh Gambhir

“…This could replace the use of bone marrow iron staining as a diagnostic tool in anemia in elderly.…”
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An unbiased cell morphology-based screen for new, biologically active small molecules. by Masahiro Tanaka, Raynard Bateman, Daniel Rauh, Eugeni Vaisberg, Shyam Ramachandani, Chao Zhang, Kirk C Hansen, Alma L Burlingame, Jay K Trautman, Kevan M Shokat, Cynthia L Adams

“…Cellular phenotypes induced by each compound were quantified by multivariate statistical analysis of the morphology, staining intensity, and spatial attributes of the cellular nuclei, microtubules, and Golgi compartments. …”
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Extraskeletal Myxoid Chondrosarcoma with Small Bowel Metastasis Causing Bowel Obstruction by Ernesto Bustinza-Linares, Francisco Socola, Vinicius Ernani, Shelly A. Miller, Jonathan C. Trent

“…It has been often described as a low-grade sarcoma although there are certain characteristics like high mitotic activity and the presence of focal regions of Ki67 staining above 25% that correlate with aggressive behavior of the tumor. …”
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Bacteriological Assessment of the Indoor Air of Different Hospitals of Kathmandu District by Arzu Kunwar, Samyukta Tamrakar, Shyaron Poudel, Sony Sharma, Pramila Parajuli

“…The bacteriological agents were isolated and identified by cultural characteristics, Gram staining, and biochemical tests, and their antibiotic susceptibility pattern was determined using CLSI Guideline, 2015. …”
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