Aortic intimal sarcoma with abdominal metastasis: case report and management approach by Gongji Yao, Gongji Yao, Jianwei Xu, Jianwei Xu

“…Post-treatment PET-CT indicated no significant tumor staining or progression.DiscussionAortic intimal sarcoma is a rare neoplasm with limited treatment options. …”
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Primary Signet Ring Cell/Histiocytoid Carcinoma of the Eyelid: Clinicopathologic Analysis with Evaluation of the E-Cadherin/β-Catenin Complex and Associated Genetic Alterations by Maria Del Valle Estopinal, Lavinia P. Middleton, Bita Esmaeli, Keyur P. Patel, Sara Nowroozizadeh, Michelle D. Williams

“…Immunohistochemically, two tumors with loss of E-cadherin expression had weak β-catenin and low cytoplasmic staining for Src while the other two cases with intact E-cadherin showed strong β-catenin expression and high cytoplasmic expression for Src. …”
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Plasma Sterilization Effectively Reduces Bacterial Contamination in Dental Unit Waterlines by Sarocha Noopan, Phattranit Unchui, Supitcha Techotinnakorn, Ruchanee Salingcarnboriboon Ampornaramveth

“…Moreover, treatment with plasma sterilization did not induce a change in the composition of microorganisms, as analyzed by Gram staining. Conclusion. Plasma sterilization, which is part of electrochemically activated water, effectively reduces bacterial contamination and reduces biofilms in dental unit waterlines.…”
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20-HETE mediates Ang II-induced cardiac hypertrophy via ROS and Ca2+ signaling in H9c2 cells by Jingyi Han, Jiaojiao Li, Lianlian Liu, Kaiyuan Li, Chun Zhang, Yong Han

“…The role of 20-HETE in Ang II-induced cardiac hypertrophy was examined using DHE, MitoSOX, and JC-1 staining to evaluate reactive oxygen species (ROS) generation and mitochondrial membrane potential changes. …”
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Efficacy of Nonpreserved Sodium Hyaluronate Artificial Tears in Dry Eye Disease Patients Treated with Prostaglandin Analogs for Primary Open-Angle Glaucoma: A Prospective, Nonrando... by Giulia Coco, Danilo Iannetta, Igino Febbraro, Ester Elmo, Gianluca Manni

“…Foreign body sensation, burning, stinging, dryness, pain, frequency of symptoms, Ocular Surface Disease Index (OSDI), conjunctival hyperaemia, corneal fluorescein staining (CFS), tear film break-up time (TBUT), best-corrected visual acuity, Schirmer test results, and 25-item National Eye Institute Visual Function Questionnaire score between the baseline and 4 and 12 weeks were evaluated. …”
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Nuclear DNA content variation within four species of Asian catfish of family Pangasidae and their two interspecific hybrids by flow cytometry by Imron, Evi Tahapari, Jadmiko Darmawan, Muhammad Luthfi Abdurachman

“…Red blood cells (RBC) were taken from the respective species/groups and NDC measurement was performed in an Attune acoustic flowcytometer (ABI) using DAPI staining and chicken, Gallus domesticus, RBC was used as size reference. …”
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YAP Overexpression in Breast Cancer Cells Promotes Angiogenesis through Activating YAP Signaling in Vascular Endothelial Cells by Yu Yan, Qiang Song, Li Yao, Liang Zhao, Hui Cai

“…Expression of proteins and phosphorylating proteins involved in YAP, G13-RhoA, and PI3K/Akt signaling pathways was evaluated using the Western blotting, immunofluorescence staining, and immunohistochemistry analysis. In addition, the effects of YAP on breast cancer angiogenesis were evaluated in vivo by tumor xenograft mice. …”
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Emodin promotes the recovery of rheumatoid arthritis by regulating the crosstalk between macrophage subsets and synovial fibroblast subsets by Lianying Cheng, Xiaofeng Rong

“…A collagen‐induced arthritis mouse model was constructed to investigate the therapeutic potential of emodin in RA by flow cytometry, micro‐CT and staining. Results Unique subsets of FLSs and STMs, namely, FAPα+THY1− FLSs, FAPα+THY1+ FLSs, and MerTKposTREM2high STMs, were identified in synovial tissues from RA patients. …”
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Histomorphometrical Study on Regional Variation in Distribution of Sweat Glands in Buffalo Skin by Debajit Debbarma, Varinder Uppal, Neelam Bansal, Anuradha Gupta

“…The paraffin sections of 5-6 μm were cut with rotary microtome and stained with hematoxylin and eosin. The sweat glands in buffaloes were of saccular and simple coiled tubular type. …”
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Upregulated Solute Carrier SLC39A1 Promotes Gastric Cancer Proliferation and Indicates Unfavorable Prognosis by Dan Yu, Yong Chen, Ming Luo, Yanjin Peng, Shengen Yi

“…A retrospective gastric adenocarcinoma cohort (n = 154) was collected from our hospital to test their tissue expression of SLC39A1 through immunohistochemical staining method. After SLC39A1 overexpression or knockdown, proliferation and invasion assays were conducted for proliferation and invasion estimation, respectively. …”
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A Novel “Slit Side View” Method to Evaluate Fluid Dynamics during Phacoemulsification by Hisaharu Suzuki, Tsutomu Igarashi, Toshihiko Shiwa, Hiroshi Takahashi

“…In this experimental study, extracted porcine eyes were injected with OVDs stained with fluorescein through a side port of the eye and then fixed on a slit lamp microscope. …”
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Impact of Notch3 Activation on Aortic Aneurysm Development in Marfan Syndrome by Kathryn Jespersen, Chenxin Li, Rishi Batra, Christopher A. Stephenson, Paul Harding, Kylie Sestak, Ryan T. Foley, Harrison Greene, Trevor Meisinger, Jason R. Cook, B. Timothy Baxter, Wanfen Xiong

“…Aortic aneurysms and rupture were evaluated with connective tissue staining, ultrasound, and life table analysis. Results. …”
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Differentially Expressed MicroRNAs in the Development of Early Diabetic Retinopathy by Qiaoyun Gong, Jia’nan Xie, Yang Liu, Ying Li, Guanfang Su

“…HREC/RPE viability decreased after 24 h incubation and diminished further over 6 days, and Hoechst staining revealed hyperglycemia-induced HREC/RPE apoptosis. …”
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Long Noncoding RNA SNHG4 Attenuates the Injury of Myocardial Infarction via Regulating miR-148b-3p/DUSP1 Axis by Sheng Wang, Zhaoyun Cheng, Xianjie Chen, Guoqing Lu, Xiliang Zhu, Gaojun Xu

“…The myocardial injury in MI rats was evaluated by echocardiography, TTC/HE/MASSON/TUNEL staining, and immunohistochemistry (Ki67). DLR assay was performed to confirm the target relationships. …”
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A Study Comparing Immunohistochemistry, Fluorescence in situ hybridization, GeneXpert® Breast Cancer STRAT4 Assay for Determining Hormone Receptor, Human Epidermal Growth Factor Re... by Rajaa El Aje, Abdellah Naya, Ayoub Khoaja, Younes Zaid, Mounia Oudghiri, Jodi Weidler, Mehdi Karkouri

“…The Xpert® Breast Cancer STRAT4, an RT-qPCR based system, can be used to classify invasive breast carcinomas based on the assessment of these four biomarkers; Methods: We evaluated ESR1, PGR, ERBB2, and MKi67 mRNA expression by Xpert Breast Cancer STRAT4 and ER, PR, HER2 and Ki67 by IHC (FISH for HER2 IHC 2+) in 200 formalin-fixed paraffin-embedded (FFPE) tissue blocks with invasive breast cancer, collected from the Pathology Department of Casablanca Ibn Rochd University Hospital; Results: Concordance between Xpert ® Breast Cancer STRAT4 and IHC was 93.5% for ER, 83.51% for PR, 95% for HER2 (92% for IHC+FISH), and 81.20% for Ki67 (excluding intermediate IHC Staining 10 ≤ %IHC <20). The simple Kappa coefficient was, for ER, 0.830 (P < 0.0001), 0.565 (P < 0.0001) for PR, 0.838 (P < 0.0001) for HER2-IHC, 0.771 (P < 0.0001) for HER2 IHC+FISH and, for, Ki67, 0.458 (P < 0.0001); Conclusions: We demonstrated globally a high concordance between centrally assessed IHC, IHC+FISH and mRNA measurements of ER/ESR1 and HER2/ERBB2, and a moderate agreement between PR/PGR and Ki67/MKi67. …”
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Gastrospheres as a Model of Gastric Cancer Stem Cells Skew Th17/Treg Balance toward Antitumor Th17 Cells by Alaleh Rezalotfi, Ghasem Solgi, Marzieh Ebrahimi

“…The proliferation was evaluated using CFSE staining; the percentages of CD4+CD25+FoxP3+ Treg and CD4+IL-17+ Th17 cells and IFN-γ+cells and the production of IL-17, TGF-β, and IL-10 were assessed by flow cytometry and ELISA, respectively. …”
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Influence of Adipose Tissue-Derived Stem Cells on the Burn Wound Healing Process by Claudio Luciano Franck, Alexandra Cristina Senegaglia, Lidiane Maria Boldrini Leite, Sérgio Adriane Bezerra de Moura, Nathália Forti Francisco, Jurandir Marcondes Ribas Filho

“…The progress was evaluated by immunohistochemical methods and H&E, Masson’s trichrome, Picrosirius red, and Lyve-1 immunofluorescence staining. Despite the quantitative similarity of blood vessels and the inflammatory infiltrate observed by H&E, there were statistically significant differences between the groups on the fourteenth day of evolution. …”
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Follow up through Endoscopical – Histological Studies and Helicobacter Pylori Infections in Patients Suffering from Gastric Ulcers by Marcos Félix Osorio Pagola, Antonio Moré Pérez, Libán Álvarez Cáceres, Juan Luis de Pasos Carrazana, Anagalys Ortega Alvelay

“…Endoscopy and biopsy of the gastric mucosa was done for the histological study of the gastric ulcers and for the diagnosis of infection due to Helicobacter pylori through hematoxiline-eosine and giemsa stains respectively. <strong><br />Results</strong>: 89 patients (92,7 %) healed their ulcers in the first three months of follow up and 5 patients underwent a histological diagnosis of malignant ulcers (5,2 %). …”
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The Protective Effect of Naringin against Bleomycin-Induced Pulmonary Fibrosis in Wistar Rats by Nergiz H. Turgut, Haki Kara, Sahende Elagoz, Koksal Deveci, Huseyin Gungor, Emre Arslanbas

“…Tissue sections were stained with hematoxylin-eosin, Masson’s trichrome, and 0.1% toluidine blue. …”
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Recurrent Herpes Zoster Ophthalmicus Preceded by Anabolic Steroids and High-Dose L-Arginine by Stephen A. LoBue, Adam Goldman, Richard A. Giovane, Stacy M. Carlson, Michael Bivona, Sinan Albear, Thomas D. LoBue

“…The best-corrected vision was 20/25 OS with slit-lamp examination remarkable for punctate staining and pseudodendrites at 6 o’clock, outside the visual axis. …”
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