Renal Tissue-Derived Exosomal miRNA-34a in Diabetic Nephropathy Induces Renal Tubular Cell Fibrosis by Promoting the Polarization of M1 Macrophages by Shuai Zheng, Yi Zeng, Liqing Chu, Taiyang Gong, Sihong Li, Min Yang

“…The DN model was established, and the success of the model establishment was confirmed by detecting general indicators, HE staining, and immunohistochemistry. Electron microscopy and NanoSight Tracking Analysis (NTA) were used to see the morphology and size of exosomes. …”
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Nanoemulsion and Encapsulation Strategy of Hydrophobic Oregano Essential Oil Increased Human Prostate Cancer Cell Death via Apoptosis by Attenuating Lipid Metabolism by Haribalan Perumalsamy, Rajeshkumar Shanmugam, Jun-Ran Kim, Gokulanathan Anandapadmanaban, Md. Amdadul Huq, Kamal Dua, Dinesh Kumar Chellappan, Tae Hyun Yoon, Sri Renukadevi Balusamy

“…The lipid biogenesis mediated, molecular target pathway analyses were performed using fluorescence cellular staining techniques, real-time RT-PCR, or western blotting analysis. …”
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Dulaglutide Protects Mice against Diabetic Sarcopenia-Mediated Muscle Injury by Inhibiting Inflammation and Regulating the Differentiation of Myoblasts by Fengyi Deng, Wenyan Wu, Xingyu Fan, Xing Zhong, Nuojin Wang, Yue Wang, Tianrong Pan, Yijun Du

“…Mouse muscle tissues were then pathologically evaluated and stained with F4/80 or MPO to detect macrophages and neutrophils, respectively. …”
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Ischemic Postconditioning Mitigates Retinopathy in Tree Shrews with Diabetic Cerebral Ischemia by Ling Zhao, Qiwei Liao, Yueting Zhang, Shufen Tan, Shuqing Li, Tingyu Ke

“…Tree shrews were divided into the normal control group, sham operation group, diabetes mellitus group, diabetes mellitus+cerebral ischemia group, and diabetes mellitus+cerebral ischemia+PC group (in which the tree shrews with diabetic cerebral ischemia were treated with ischemic PC). H&E staining was used to examine the pathological changes in the retina, and immunohistochemistry was performed to determine the retinal expression of VEGF (vascular endothelial growth factor). …”
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Absence of Tuberculosis-Causing Mycobacteria from Slaughtered Livestock Tissues and Environmental Samples, Gauteng Province, South Africa by Vuyokazi Mareledwane, Abiodun A. Adesiyun, Tiny M. Hlokwe

“…Additionally, 19 environmental samples were collected from feedlots, or where animals drink water while awaiting slaughter, to identify mycobacterial species using culture, acid-fast bacteria staining, and polymerase chain reaction (PCR). The Chi-square and Fisher’s Exact tests were used to detect statistically significant differences in the frequency of detection of Mycobacterium spp. according to the variables investigated (types of tissues, livestock, abattoirs, etc.). …”
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Very Early-Onset Inflammatory Bowel Disease (VEO-IBD) Presenting with Recurrent Leukocytoclastic Vasculitis Preceded by Streptococcal Pharyngitis by Ashley Fonseca, Julee Sunny, Lina M. Felipez

“…Prior to the diagnosis of VEO-IBD, a skin biopsy was obtained and had shown leukocytoclastic vasculitis with a negative IgA stain. Initial laboratory results were remarkable for leukocytosis and increased anti-strep O and anti-DNase B titers. …”
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Basis for the Age-related Decline in Intestinal Mucosal Immunity by Douglas L. Schmucker, Robert L. Owen, Robert Outenreath, Karine Thoreux

“…This was accompanied by a >17% decrease in the areal density of LP blood vessels staining positive for the cell adhesion molecule MAdCAM-1. …”
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Detection of Complement C1q B Chain Overexpression and Its Latent Molecular Mechanisms in Cervical Cancer Tissues Using Multiple Methods by Si-Tong Lin, Zi-Qian Liang, Xiao-Yu Chen, Xin-Qing Ye, Yu-Yan Pang, Jia-Yuan Luo, Jun-Hong Chen, Yi-Wu Dang, Gang Chen

“…In total, 120 cervical cancer tissues, as well as 20 samples each of high-grade squamous intraepithelial lesions (HSILs), low-grade squamous intraepithelial lesions (LSILs), and benign cervical tissue, were collected to evaluate the expression of C1QB protein via immunohistochemical staining. We conducted an integrated analysis of C1QB mRNA expression in cervical cancer using public microarrays and RNA-seq data sets by calculating standard mean differences (SMDs). …”
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Decreased Breg/Th17 Ratio Improved the Prognosis of Patients with Ulcerative Colitis by Xue Bing, Liang Linlang, Chen Keyan

“…Peripheral blood and colonic mucosa were collected from patients with ulcerative colitis. Hematoxylin-eosin staining was used to observe the pathological changes of colonic mucosa. …”
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lncRNA FR215775 Regulates Th2 Differentiation in Murine Allergic Rhinitis by Yue Ma, Le Shi, Keqing Zhao, Chunquan Zheng

“…Then, hematoxylin and eosin, Alcian blue-periodic acid Schiff, and toluidine blue staining were performed, and the levels of IL-2, IL-4, IL-5, IL-6, IL-10, IL-17A, IFN-γ, and TNF in the AR mice were also determined. …”
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Transient ocular hypertension remodels astrocytes through S100B. by Weiran Huang, Kenji Matsushita, Rumi Kawashima, Susumu Hara, Yuichi Yasukura, Kaito Yamaguchi, Shinichi Usui, Koichi Baba, Andrew J Quantock, Kohji Nishida

“…Glial fibrillary acidic protein (GFAP), S100B, and actin staining were used to characterize astrocyte morphology and cytoskeleton, with qPCR used to measure mRNA expression. …”
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Role of Leptin/Osteopontin Axis in the Function of Eosinophils in Allergic Rhinitis with Obesity by Wenlong Liu, Qingxiang Zeng, Yanqiu Chen, Ren Zhong Luo

“…Obese AR mice presented with more severe eosinophilia and symptoms compared with nonobese AR mice or control mice. Immunochemistry staining of leptin and OPN of nasal turbinate in obese AR mice was also stronger than those in nonobese AR mice or control mice. …”
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Neonatal Sepsis in a Resource-Limited Setting: Causative Microorganisms and Antimicrobial Susceptibility Profile by Edna Nai Acheampong, Joseph Adjei Tsiase, Daniel Kwame Afriyie, Seth Kwabena Amponsah

“…Bacteria isolates were identified by Gram staining and conventional biochemical methods. Antimicrobial susceptibility testing was done by Kirby–Bauer’s disc diffusion method, and interpretations were carried out according to clinical and laboratory standards. …”
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Effects of traditional Chinese medicine Zuo-Gui-Wan on gut microbiota in an osteoporotic mouse model by Junjie Li, HaomingYou, Yucheng Hu, Ruxu Li, Tianxin Ouyang, Qiang Ran, Guilong Zhang, Yong Huang

“…Subsequently, Micro-CT, HE staining, and analysis of gut microbiota were conducted to further explore the potential mechanism. …”
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RETRACTED ARTICLE: Emodin enhances osteogenesis and inhibits adipogenesis by Feng Yang, Pu-wei Yuan, Yang-Quan Hao, Zheng-Mao Lu

“…In vitro, we analyze the effect of emodin—at concentrations between 0.1 μM and 10 μM-on the processes of inducing osteogenesis and inhibiting adipogenesis in BMSCs by ALP, Oil red O staining, real time RT-PCR and western blot. Results As our experiment shows that emodin could increase the number of osteoblast, BMD (bone mineral density), BV/TV (trabecular bone volume fraction), Tb.N (trabecular number) and Conn.D (connectivity density) of OVX (ovariectomized) mice and decrease the bone marrow fat tissue and adipocytes. …”
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Cebpb Regulates Skeletal Stem Cell Osteogenic Differentiation and Fracture Healing via the WNT/β-Catenin Pathway by Jiansong Wang, Chensong Yang, Fanyu Kong, Zhi Zhang, Shengchao Ji, Guixin Sun

“…Alkaline phosphatase assay and alizarin red S staining were used to illustrate the osteogenic differentiation ability of SSCs in vitro. …”
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Catholic Church Interiors in Fin-de-Siècle Literature by Claire Masurel-Murray

“…The works of such late-Victorian writers as Lionel Johnson, Ernest Dowson, Theodore Wratislaw, John Oliver Hobbes and Oscar Wilde represent Catholic churches as retreats set apart from the ugliness and mediocrity of Victorian England—religious versions of Des Esseintes’s Fontenay-aux-Roses house in À rebours—filled with incense, organ music, and coloured light filtering through medieval stained-glass windows. In Dowson’s correspondence or in his poem ‘Benedictio Domini’, in Johnson’s ‘Our Lady of France’, in Wratislaw’s ‘Palm Sunday’ and ‘Songs to Elizabeth’, in some of Wilde’s stories, the opposition between inside and outside expresses figuratively the fundamental incompatibility between an ideal of beauty, embodied in the aesthetic experience of the church, and the coarseness of the outside world. …”
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Developmental and hormonal regulation of Arabidopsis thaliana ornithine-delta-aminotransferase by A. A. Egorova, S. V. Gerasimova, A. V. Kochetov

“…Plants were incubated with staining solution containing X-Gluc. We show that under standard growth conditions, the promoter is active during germination and in developing floral organs. …”
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Perkembangan Profesional Konselor Untuk Memenuhi Kebutuhan Masyarakat Industri by Fadhila Yusri

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Electroacupuncture Alleviates Neuropathic Pain through Regulating miR-206-3p Targeting BDNF after CCI by Wenzhan Tu, Jingjing Yue, Xuqing Li, Qiaoyun Wu, Guanhu Yang, Shengcun Li, Qiangsan Sun, Songhe Jiang

“…Mechanical withdrawal threshold (MWT) and thermal withdrawal latency (TWL) tests were used to determine differences in neurobehavioral manifestations. qPCR, western blotting, and immunofluorescence (IF) were carried out to detect the expression levels of miR-206-3p, BDNF, BAX/Bcl-2, TNF-α, and IL-6. Nissl staining was measured to observe morphological changes in neurons. …”
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