Automated quantification of axonal and myelin changes in contusion, dislocation, and distraction spinal cord injuries: Insights into targeted remyelination and axonal regeneration by Xuan Li, Yuan He, Fangyao Chen, Xin Tong, Yunlong Fan, Yuzhe Langzhou, Jie Liu, Kinon Chen

“…Spinal cords were processed and stained for neurofilament, tubulin, and myelin basic protein, with histology images segmented into dorsal, lateral, and ventral white matter regions. …”
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Mechanism of engineered macrophage membrane bionic gene-carrying nanospheres for targeted drug delivery to promote wound repair in deep second-degree burns by Zhihan Zhu, Xinghua Zhu, Shichen Miao, Bolin Wang, Zihan Li, Dinghao Zhang, Shentian Zou, Yi Zhang, Qingrong Zhang, Kesu Hu

“…Twenty-seven Sprague–Dawley rats were randomly divided into three groups: a saline control (NS) group, an engineered macrophage membrane-encapsulated nanospheres (ETMM@NPS) group, and an engineered macrophage membrane-encapsulated nanospheres treatment with HGF-loaded gene (HGF@ETMM@NPS) group.The wound tissue sections were examined histologically using hematoxylin and eosin (H&E) and Masson trichrome staining. Immunohistochemistry and Western blotting were performed to determine the expression of relevant proteins. …”
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Development of Cutaneous Leishmaniasis after Leishmania Skin Test by Paulo R. Machado, Augusto M. Carvalho, Gustavo U. Machado, Marina L. Dantas, Sérgio Arruda

“…Further analysis identified amastigotes by immunohistochemical stain. Mononuclear cells harvested from the patient were stimulated with Leishmania antigen and showed high levels of production of both tumor necrosis factor-alpha (TNF-α) and interferon-gamma (IFN-γ): 2,943 pg/mL and 2,313 pg/mL, respectively. …”
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Sodium Lactate Accelerates M2 Macrophage Polarization and Improves Cardiac Function after Myocardial Infarction in Mice by Jialiang Zhang, Fangyang Huang, Li Chen, Guoyong Li, Wenhua Lei, Jiahao Zhao, Yanbiao Liao, Yijian Li, Changming Li, Mao Chen

“…The cardiac fibrosis area was assessed by Masson trichrome staining. Macrophage phenotype was detected via qPCR, flow cytometry, and immunofluorescence. …”
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Experimental Study on the Shear Behavior of Bolted Concrete Blocks with Oblique Shear Test by Bo Meng, Hongwen Jing, Shengqi Yang, Yingchao Wang, Biao Li

“…More than 90 intact cubic samples (100 mm × 100 mm × 100 mm) with bolts ranging from 2 mm to 5 mm in diameter were tested at a constant stain rate of 0.5 mm/min. An oblique shear apparatus, which could simultaneously apply shear and normal force on tested samples at three slope angles (53°, 58°, and 63°) of a predetermined shear plane, was employed. …”
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Smad4 deficiency in hepatocytes attenuates NAFLD progression via inhibition of lipogenesis and macrophage polarization by Wei Yang, Xuanxuan Yan, Rui Chen, Xin Xin, Shuang Ge, Yongxiang Zhao, Xinlong Yan, Jinhua Zhang

“…The role of Smad4 in the occurrence and development of NAFLD was determined by monitoring the body weight of mice, detecting triglycerides and free fatty acids in serum and liver tissue homogenates, staining the tissue sections to observe the accumulation of liver fat, and RT-qPCR detecting the expression of genes related to lipogenesis, fatty acid intake, and fatty acid β oxidation. …”
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Genome resource banking in the family Felidae by S. Ya. Amstislavsky, V. I. Mokrousova, V. V. Kozhevnikova, E. A. Kizilova, E. Yu. Brusentsev, K. A. Okotrub, V. A. Naprimerov, S. V. Naidenko

“…Different methods of frozen-thawed spermatozoa and embryonic viability testing were used in this study, including vital staining and subsequent fluorescent and light microscopy, and heterologous in vitro fertilization.…”
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NADPH Oxidase Inhibitor Apocynin Attenuates PCB153-Induced Thyroid Injury in Rats by Ablikim Abliz, Chen Chen, Wenhong Deng, Weixing Wang, Rongze Sun

“…NOX2, 8-OHdG, and NF-κB expression in the thyroid tissue was evaluated by immune-histochemical staining. Oxidative stress and inflammatory cytokines were detected. …”
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Effects of Stromal Cell-Derived Factor-1α Secreted in Degenerative Intervertebral Disc on Activation and Recruitment of Nucleus Pulposus-Derived Stem Cells by Jinwei Ying, Zhihua Han, Shishen Pei, Linghao Su, Dike Ruan

“…Immunohistochemical staining also showed that the expression level of SDF-1α was significantly higher in the degenerative group compared to that in the normal group. …”
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Extensive Characterization of Mesenchymal Stem Cell Marker Expression on Freshly Isolated and In Vitro Expanded Human Adipose-Derived Stem Cells from Breast Cancer Patients by Premrutai Thitilertdecha, Visnu Lohsiriwat, Poonsin Poungpairoj, Varangkana Tantithavorn, Nattawat Onlamoon

“…This study, therefore, developed a polychromatic staining panel for an in-depth characterization of freshly isolated SVF and expanded adipose-derived stem cells (ADSC) from the patients. …”
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Electroacupuncture for mild-to-moderate dry eye: study protocol for a multicentre, randomised, single-blind, sham-controlled trial by Yue Zhao, Guang Yang, Yun-Qiong Lu, Mei-Yan Li, Jue Hong, Yan-Ting Yang, Xue-Jun Wang, Xie-He Kong, Zhao Ma, Xin-Yun Huang, Yue-Lan Zou, Xing-Tao Zhou, Xiao-Peng Ma

“…The secondary outcomes include tear meniscus height, the Schirmer I test, corneal and conjunctival sensation, the ocular surface disease index, corneal fluorescein staining, the numerical rating scale and the Chinese DE-related quality of life scale.Ethics and dissemination The trial protocol and informed consent were approved by the Ethics Committee of Yueyang Hospital of Integrated Traditional Chinese and Western Medicine Affiliated to Shanghai University of Traditional Chinese Medicine (identifier: 2021–119), Shanghai Eye Disease Prevention and Treatment Center (identifier: 2022SQ003) and Eye and ENT Hospital of Fudan University (identifier: 2022014).Trial registration number NCT05552820.…”
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Effects of IQW and IRW on Inflammation and Gut Microbiota in ETEC-Induced Diarrhea by Naiyuan Liu, Lingsi Zhou, Jun Fang, Hongmei Jiang, Gang Liu

“…Villus length and crypt depth were measured after hematoxylin and eosin staining. The inflammatory reaction was analyzed via inflammatory cytokines (i.e., TNF-α, IL-1β, IL-6, and IL-10) using the enzyme-linked immunosorbent assay (ELISA). …”
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Skeletal Muscle-Derived Stem/Progenitor Cells: A Potential Strategy for the Treatment of Acute Kidney Injury by Egle Pavyde, Romaldas Maciulaitis, Mykolas Mauricas, Gintaras Sudzius, Ernesta Ivanauskaite Didziokiene, Arvydas Laurinavicius, Neringa Sutkeviciene, Edgaras Stankevicius, Justinas Maciulaitis, Arvydas Usas

“…Characteristics of rat BM-MSCs and MDSPCs were assessed by population doubling time, flow cytometry, immunofluorescence staining, RT-PCR, and multipotent differentiation capacity. …”
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Nε-Carboxymethyl-Lysine Negatively Regulates Foam Cell Migration via the Vav1/Rac1 Pathway by Zhengyang Bao, Lili Zhang, Lihua Li, Jinchuan Yan, Qiwen Pang, Zhen Sun, Yue Geng, Lele Jing, Chen Shao, Zhongqun Wang

“…Transwell and wound healing assays showed that the migratory ability of macrophage-derived foam cells was inhibited by CML. Cytoskeletal staining showed that advanced glycation end products inhibited the formation of lamellipodia in foam cells, and inhibition of the Vav1/Rac1 pathway restored the formation of lamellipodia. …”
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Loss of flavonoids homeostasis leads to pistillody in sua-CMS of Nicotiana tabacum by Jie Xu, Zhuo Wei, Jugou Liao, Keliang Tao, Junpeng Zhang, Yu Jiang, Yongzhi Niu, Yunye Zheng, Limeng Zhang, Xuemei Wei

“…Chemical fluorescence staining assay showed that reactive oxygen species (ROS) levels were higher in the pistil-like structure of sua-CMS. …”
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Analytical Method for the Validation of Three Polyphenols as a Marker Compound for the Standardization of Solidago virgaurea subsp. gigantea Extracts and Antiadipogenesis of Harves... by Seung Hwan Hwang, Ju Hee Kim, Zhiqiang Wang, Jae-Yong Lee, Soon Sung Lim

“…The antiadipogenesis activity of SV extracts collected from five different months and from seven different regions was evaluated using an Oil Red O staining assay in 3T3-L1 cells. Extract from SV collected in April from the Ulleung Island produced over 106.89% inhibition of adipogenesis without cytotoxicity at 50 μg/ml. …”
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Regeneration of Tracheal Tissue in Partial Defects Using Porcine Small Intestinal Submucosa by Nelson Bergonse Neto, Lianna Ferrari Jorge, Julio C. Francisco, Bruna Olandoski Erbano, Barbara Evelin Gonçalves Barboza, Larissa Luvison Gomes da Silva, Marcia Olandoski, Katherine Athayde Teixeira de Carvalho, Luiz Felipe Pinho Moreira, Jose Rocha Faria Neto, Eltyeb Abdelwahid, Luiz Cesar Guarita-Souza

“…We examined tissues at the site of tracheostomy 60 days after surgery using histological analysis with hematoxylin and eosin (H&E) staining and analyzed the perimeter and area of the defect with Image-Pro® PLUS 4.5 (Media Cybernetics). …”
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Establishing a Surgical Procedure for Rhesus Epiretinal Scaffold Implantation with HiPSC-Derived Retinal Progenitors by Ziming Luo, Kang Li, Kaijing Li, Bikun Xian, Ying Liu, Sijing Yang, Chaochao Xu, Zhigang Fan, Shoutao Lu, Haijun Zhang, Jian Ge

“…Follow-up examination showed that retinal tack fixation was the only method to maintain the scaffolds attached to host retina for at least 3 weeks, which is the minimal time required for cell integration. Histological staining demonstrated slight glial fibrillary acidic protein (GFAP) accumulation in the retinal tack insertion area. …”
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Differential Effects of Chronic Pulsatile versus Chronic Constant Maternal Hyperglycemia on Fetal Pancreatic β-Cells by Mackenzie S. Frost, Aqib H. Zehri, Sean W. Limesand, William W. Hay, Paul J. Rozance

“…We measured the impact of one week of chronic constant hyperglycemia (CHG, n=6) versus pulsatile hyperglycemia (PHG, n=5) versus controls (n=7) on the percentage of the fetal pancreas staining for insulin (β-cell area), mitotic and apoptotic indices and size of fetal β-cells, and fetal insulin secretion in sheep. …”
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Effect of ectoparasite Ichthyophthirius multifiliis on the histopathology and gill and gut microbiota of goldfish (Carassius auratus) by Xialian Bu, Xialian Bu, Xianqi Peng, Xianqi Peng, Lei Huang, Lei Huang, Yu Zhao, Jinbiao Jiao, Jinbiao Jiao, Jian Zhu, Jing Chen, Jing Chen, Xiaohong Huang, Xiaohong Huang, Aqin Zheng, Aqin Zheng, Huantao Qu, Jiayun Yao, Jiayun Yao

“…However, whether it influences gill and gut microbiota is still unknow.MethodsIn this study, H&E staining was used to show the gill and gut histopathological characteristics of I. multifiliis-infected and uninfected goldfish (Carassius auratus). …”
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