Clinicopathological and Immunohistochemical Characterisation of Gastric Schwannomas in 29 Cases by Lijun Zheng, Xiaojiang Wu, Martin E. Kreis, Zhen Yu, Lijin Feng, Chunqiu Chen, Bin Xu, Zhaode Bu, Ziyu Li, Jiafu Ji

“…All tumors were positive for S-100 and CD56 proteins, displaying a diffuse staining pattern. Vimentin was expressed in 100% cases and all schwannomas were negative for smooth muscle actin, c-kit, and HMB-45. …”
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Lack of Effect of H2-Receptor Antagonists and Antacids on the Gastric and Duodenal Gastrin-, Somatostatin- and Serotonin-Producing Cells in Patients with Acid Peptic Disorders by WR Yacoub, ABR Thomson, P Hooper, LD Jewell

“…Seven patients with minimal dyspeptic symptoms and an endoscopically and histologically normal stomach and duodenum served as controls. Immunoperoxidase staining for gastrin-producing G cells, somatostatin-producing D cells and serotonin-producing EC cells was carried out on fundic, antral and duodenal biopsies, and quantitated using a Zeiss MOP videoplan. …”
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A Diet Enriched in Docosahexanoic Acid Exacerbates Brain Parenchymal Extravasation of Apo B Lipoproteins Induced by Chronic Ingestion of Saturated Fats by Menuka M. Pallebage-Gamarallage, Virginie Lam, Ryusuke Takechi, Susan Galloway, John C. L. Mamo

“…Cerebrovascular leakage of apo B was quantitated utilising immunofluorescent staining. The plasma concentration of brain-derived S100𝛽 was measured as a marker of cerebrovascular inflammation. …”
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Comparisons of Retinal Nerve Fiber Layer Thickness after Indocyanine Green, Brilliant Blue G, or Triamcinolone Acetonide-Assisted Macular Hole Surgery by Yoshiharu Toba, Shigeki Machida, Daijiro Kurosaka

“…The degree of change of the RNFL thickness was not significantly related to the type of vital stain used during MH surgery.…”
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The Degeneration and Apoptosis Patterns of Cone Photoreceptors in rd11 Mice by Hua Zhang, Xia Li, Xufeng Dai, Juanjuan Han, Yangyang Zhang, Yan Qi, Ying He, Yan Liu, Bo Chang, Ji-jing Pang

“…The distribution patterns of cone function-related L- and S-opsin were examined by immunofluorescence staining, and the apoptosis was performed by TUNEL assay in rd11 mice. …”
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Applying reproductive technologies and genome resource banking to laboratory animals by S. Ya. Amstislavsky, E. Yu. Brusentsev, T. O. Abramova, D. S. Ragaeva, I. N. Rozhkova, T. N. Igonina, E. A. Kizilova, V. A. Naprimerov, N. Yu. Feoktistoiva

“…Furthermore, semen of Djungarian (Phodopus sungorus) and Campbell’s (Phodopus campbelli) hamsters, domestic cat (Felis catus), amur cat (Prionailurus bengalensis euptilurus) and bobcat (Lynx rufus) was frozen and cryopreserved. Double staining by SYBR Green/PI and subsequent confocal microscopy demonstrated that more than 40 % of amur cat semen retained viability after cryopreservation. …”
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Effect of agarose/gelatin gel addition on the pro-angiogenic potential of polyhydroxybutyrate/chitosan scaffolds by Mária Giretová, Ľubomír Medvecký, Zuzana Demčišáková, Lenka Luptáková, Eva Petrovová, Radoslava Štulajterová

“…No sign of cytotoxicity was identified by both the MTS assay and live/dead cell staining. The quail chorioallantoic membrane (CAM) assay—as an alternative to in vivo assays—revealed suitable pro-angiogenic properties of the scaffold for the formation and ingrowth of new blood vessels. …”
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IL-1β Suppresses the Formation of Osteoclasts by Increasing OPG Production via an Autocrine Mechanism Involving Celecoxib-Related Prostaglandins in Chondrocytes by Yusuke Watanabe, Aki Namba, Yukiko Aida, Kazuhiro Honda, Hideki Tanaka, Naoto Suzuki, Hideo Matsumura, Masao Maeno

“…Conditioned medium from IL-1β-treated chondrocytes decreased TRAP staining in RAW264.7 cells. These results suggest that IL-1β suppresses the formation of osteoclast-like cells via increased OPG production and decreased M-CSF production in chondrocytes, and OPG production may increase through an autocrine mechanism involving celecoxib-related PGs.…”
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Andrographolide suppresses cervical cancer progression by targeting angiogenesis and inducing apoptosis in a CAM-PDX model by Wanwan Zou, Jun Lou, Yun Yi, Yiming Cui, Xiaoyan Chu

“…The model was validated through hematoxylin-eosin staining and immunohistochemistry, which confirmed its ability to accurately replicate the histological and molecular characteristics of patient-derived xenografts, establishing its reliability for therapeutic screening. …”
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Costimulation of Murine Osteoblasts with Interferon-γ and Tumor Necrosis Factor-α Induces Apoptosis through Downregulation of Bcl-2 and Release of Cytochrome c from Mitochondria by Mayumi Iguchi, Miki Hiroi, Haruhide Kanegae, Yoshihiro Ohmori

“…The diminished cell viability was due to apoptosis, as indicated by increased TUNEL staining and elevated caspase 3, 8, and 9 activities. …”
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Protective effects of 18β-glycyrrhetinic acid on Pasteurella multocida–induced vascular inflammatory injury in mice by Qirong Lu, Qirong Lu, Luyao Wang, Luyao Wang, Xueping Jiang, Xueping Jiang, Wantong Han, Wantong Han, Pu Guo, Pu Guo, Yu Liu, Yu Liu, Shulin Fu, Shulin Fu, Jianglin Xiong, Jianglin Xiong, Zhongyuan Wu, Zhongyuan Wu, Yinsheng Qiu, Yinsheng Qiu

“…Haematoxylin and eosin staining revealed that GA delayed the progression of vascular injury, including abnormalities in elastic fibres, local rupture of the vascular intima, and inflammatory cell infiltration in response to Pm infection. …”
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Adjusting Effects of Baicalin for Nuclear Factor-κB and Tumor Necrosis Factor-α on Rats With Caerulein-Induced Acute Pancreatitis by Dongbo Xue, Weihui Zhang, Yingmei Zhang, Haiyang Wang, Biao Zheng, Xingye Shi

“…Pathological damage of pancreatic tissue was scored with hematoxylin and eosin (HE) staining. The mRNA expression of TNF-α was measured with semiquantitative RT-PCR, and activation of NF-κB was detected with flow cytometry assay. …”
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LRP11 facilitates lipid metabolism and malignancy in hepatocellular carcinoma by stabilizing RACK1 through USP5 regulation by Litao Liang, Wenbo Jia, Jinyi Wang, Yanzhi Feng, Deming Zhu, Wenhu Zhao, Chao Xu, Xiangyu Ling, Qingpeng Lv, Xiaoming Ai, Lianbao Kong, Wenzhou Ding

“…Lipid metabolism plays a crucial role in the metabolic reprogramming observed in HCC, although the underlying mechanisms are still being elucidated. Nile red staining and lipid assays showed that LRP11 knockdown significantly reduces lipid accumulation in HCC cells, with a concurrent decrease in key lipid metabolism markers such as FSAN, ACLY and ACSL4, as demonstrated by Western blotting. …”
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AI-based tumor-infiltrating lymphocyte scoring system for assessing HCC prognosis in patients undergoing liver resection by Zhiyang Chen, Tingting Xie, Shuting Chen, Zhenhui Li, Su Yao, Xuanjun Lu, Wenfeng He, Chao Tang, Dacheng Yang, Shaohua Li, Feng Shi, Huan Lin, Zipei Li, Anant Madabhushi, Xiangtian Zhao, Zaiyi Liu, Cheng Lu

“…Conclusions: We developed an automated system using CD8-stained whole-slide images to assess immune infiltration (ATLS-8). …”
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Correlation of the Changes of Gray Matter Volume with Aβ Deposition and Learning and Memory Abilities in APP/PS1 Mice at Different Periods by Yuhao ZHANG, Hao JIN, Xiufeng ZHANG, Shengxiang LIANG, Weilin LIU, Jing TAO, Lidian CHEN

“…The brain paraffin sections of two groups of mice of different months were separated and prepared, and the Aβ deposition in bilateral entorhinal cortex and hippocampus of the two groups was detected by Thioflavine-s fluorescence staining, and the correlation between the changes of gray matter volume and memory learning ability and Aβ deposition in the corresponding brain area of the two groups was analyzed by Pearson correlation analysis.Results:①The results of Morris water maze test: compared with the WT group, there was no significant difference in the behavioral results of the 2-month-old mice in the APP/PS1 group, and the difference was not statistically significant (<italic>P</italic>&gt;0.05); the escape latency of mice of the APP/PS1 group at the 6 and 12 month-old increased significantly, the number of crossing platforms of the APP/PS1 group at the 6 and 12 month-old decreased significantly, and the difference was statistically significant (<italic>P</italic>&lt;0.05).②The results of MRI T2WI scan: compared with the WT group, there was no significant change in the gray matter volume of the APP/PS1 group at 2-month-old, and the difference was not statistically significant (<italic>P</italic>&gt;0.05); the volume of the entorhinal cortex of the APP/PS1 group at 6-month-old decreased significantly, and the volume of the entorhinal cortex and hippocampus of the APP/PS1 group at 12-month-old mice decreased significantly, the difference was statistically significant (<italic>P</italic>&lt;0.05).③The results of Th-s fluorescence staining test: Aβ deposition was not detected in WT group and APP/PS1 group at 2-month-old; compared with the WT group, a small amount of Aβ deposition could be detected in the entorhinal cortex and hippocampus of the APP/PS1 group at 6-month-old, while a large number of dense Aβ plaques can be detected in the entorhinal cortex and hippocampus of the APP/PS1 group at 12-month-old.④Correlation analysis results: APP/PS1 group mice's entorhinal cortex and hippocampus volume changes were significantly negatively correlated with escape latency (<italic>r</italic>=-0.729, <italic>P</italic>&lt;0.001; <italic>r</italic>=-0.643, <italic>P</italic>&lt;0.001); the volume changes of the entorhinal cortex and hippocampus in the APP/PS1 group showed a positive correlation with the number of crossing platforms (<italic>r</italic>=0.705, <italic>P</italic>&lt;0.001; <italic>r</italic>=0.719, <italic>P</italic>&lt;0.001); the volume changes of the entorhinal cortex and hippocampus in the APP/PS1 group were significantly negatively correlated with Aβ deposition (<italic>r</italic>=-0.865, <italic>P</italic>&lt;0.001; <italic>r</italic>=-0.885, <italic>P</italic>&lt;0.001).Conclusion:APP/PS1 transgenic mice may have learning and memory dysfunction at 6-month-old, and it gradually worsen with time; the learning and memory dysfunction may be related to the atrophy of the entorhinal cortex and hippocampus in the brain of mice, and excessive Aβ deposition may be the one of the factors that cause the brain gray matter volume to shrink.…”
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NUCB2/Nesfatin-1 Regulation of Chronic Visceral Hyperalgesia by Qiaoyan Gu, Yuan Lei, Jianming Wu, Ting He, Juanjuan Li, Shanshan Song

“…The models were identified by the distension volume threshold to reach abdominal withdraw reflex AWR score=3, histological staining, and myeloperoxidase (MPO) detection. The visceral sensitivity to chronic visceral hyperalgesia was then evaluated. …”
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Myotubularin-Related Phosphatase 3 Promotes Growth of Colorectal Cancer Cells by Bo’an Zheng, Xiaojun Yu, Rui Chai

“…The effect of MTMR3 knockdown on cell apoptosis was evaluated by flow cytometry with Annexin V/7-AAD double staining. The activation of apoptotic markers, Bad and PARP, was detected using Intracellular Signaling Array. …”
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Effect of slope position on the spatial distribution of soil preferential flow paths based on point pattern analysis. by Mingfeng Li, Jingjing Yao, Ru Yan, Yingxu Fan, Shuqin Liu

“…Therefore, taking the subtropical evergreen broad-leaved forest area of Simian Mountain as the study area, this study conducted a quantitative analysis of the spatial distribution characteristics and spatial association of soil preferential flow at different slope positions (slope shoulder (SS), mid-slope (SM) and slope foot (SF)) using field staining tracer tests and spatial point pattern analysis. …”
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EZH2 Regulates Lipopolysaccharide-Induced Periodontal Ligament Stem Cell Proliferation and Osteogenesis through TLR4/MyD88/NF-κB Pathway by Pengcheng Wang, Huan Tian, Zheng Zhang, Zuomin Wang

“…The formation of mineralized nodules was analyzed using alizarin red staining. Results. LPS stimulation significantly promoted EZH2 and H3K27me3 expression in primary human periodontal ligament stem cells (PDLSCs). …”
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Alpiniae oxyphyllae Fructus ameliorates renal lipid accumulation in diabetic kidney disease via activating PPARα by Zi-Jie Yan, Lin Zhang, Xin-Yao Han, Yu Kang, Shu-Man Liu, Tian-Peng Ma, Man Xiao, Yi-Qiang Xie

“…Cell viability and lipid accumulation were detected by CCK8 and oil red O staining. The expressions of PPARα and fatty acid oxidation-related genes (ACOX1 and CPT1A) were detected by quantitative RT-PCR, Western blot, and immunofluorescence. …”
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