Rotavirus VP6 Adjuvant Effect on Norovirus GII.4 Virus-Like Particle Uptake and Presentation by Bone Marrow-Derived Dendritic Cells In Vitro and In Vivo by Kirsi Tamminen, Suvi Heinimäki, Timo Vesikari, Vesna Blazevic

“…The adjuvant effect of VP6 on GII.4 VLP presentation and NoV-specific immune response induction by BMDC in vivo was also studied. Intracellular staining demonstrated that BMDCs internalized both antigens, but VP6 more efficiently than NoV VLPs. …”
Get full text

Nasal Carriage of Methicillin-Resistant Staphylococcus aureus among Health Care Workers in Tertiary and Regional Hospitals in Dar es Salam, Tanzania by Agricola Joachim, Sabrina J. Moyo, Lillian Nkinda, Mtebe Majigo, Sima Rugarabamu, Elizabeth G. Mkashabani, Elia J. Mmbaga, Naboth Mbembati, Said Aboud, Eligius F. Lyamuya

“…S. aureus was identified based on colonial morphology, Gram staining, catalase, coagulase, and DNase test results. …”
Get full text

Tuberculosis Mimicking-Musculoskeletal Tumor of The Hand: An Uncommon Case of Extrapulmonary Tuberculosis by Andy Lesmana, Hapsari Retno Dewanti, Lenty BR Perangin Angin, Muhamad Irvan Muzakky, Stefi Geovani Valentin Hayon, Mentari Maratus Sholihah, Muhammad Ifham Hanif

“…The histopathology study revealed a chronic granulomatous structure surrounded by epitheloid cells, datia Langhans cells, and caseating necrosis. The acid-fast staining confirmed the presence of bacilliform bacteria. …”
Get full text

Negative Feedback of the cAMP/PKA Pathway Regulates the Effects of Endoplasmic Reticulum Stress-Induced NLRP3 Inflammasome Activation on Type II Alveolar Epithelial Cell Pyroptosis... by Qiaohui Hong, Yue Zhang, Weixian Lin, Wei Wang, Yafei Yuan, Jiajia Lin, Zhanzhan Xie, Xu Li, Ying Meng

“…In addition, immunofluorescence staining further confirmed that ER stress, NLRP3 inflammasome, and cAMP/PKA had potential links on type II AECs in BLM group. …”
Get full text

Concordance between Immunohistochemistry and Microarray Gene Expression Profiling for Estrogen Receptor, Progesterone Receptor, and HER2 Receptor Statuses in Breast Cancer Patients... by Ghina B. Fakhri, Reem S. Akel, Maya K. Khalil, Deborah A. Mukherji, Fouad I. Boulos, Arafat H. Tfayli

“…Immunohistochemical staining for estrogen receptor, progesterone receptor, and HER2 status was performed using standard methods at a CAP-accredited pathology laboratory. …”
Get full text

Local delivery of TGF-β1-mRNA decreases fibrosis in osteochondral defects by Gianluca Fontana, Brett Nemke, Yan Lu, Connie Chamberlain, Jae-Sung Lee, Joshua A. Choe, Hongli Jiao, Michael Nelson, Margot Amitrano, Wan-Ju Li, Mark Markel, William L. Murphy

“…At 9 weeks post-surgery, the mRNA-treated defects had a superior macroscopic cartilage appearance, decreased type I collagen deposition, increased stain intensity for type II collagen and increased glycosaminoglycan deposition area when compared to the controls. …”
Get full text

Expression of Sclerostin in Osteoporotic Fracture Patients Is Associated with DNA Methylation in the CpG Island of the SOST Gene by Yanming Cao, Bin Wang, Ding Wang, Dongxiang Zhan, Caiyuan Mai, Peng Wang, Qiushi Wei, Yamei Liu, Haibin Wang, Wei He, Liangliang Xu

“…Also, immunohistochemical staining was performed to observe the expression of SOST protein in the bone samples. …”
Get full text

Effects of Ethyl Pyruvate on Bile Duct Ligation-Induced Liver Fibrosis by Regulating Nrf2 Pathway and Proinflammatory Cytokines in Rats by Yonghua Zong, Mingxiao Zhang, Shuai Li, Wenqian Qi, Juan Li, Tonghua Liu, Huijun Yang, Chen Lu, Xiaosong Hu

“…Liver fibrosis was induced by common bile duct ligation and was evaluated by serum biochemical parameter levels, Masson’s trichrome staining, α-SMA expression, and collagen I deposition. …”
Get full text

Effect of Bacterial Nanocellulose with Chemisorbed Antiseptics on Alveolar Bone Repair in Rats Undergoing Bisphosphonate Therapy by Marcelo Matos Rocha, Valesca Sander Koth, Marcela Wiltgen Jeffman, Fernanda Gonçalves Salum, Josiane de Almeida, Karina Cesca, Karen Cherubini

“…BNC/Iodine showed greater CD31 immunostaining compared to BNC and the Control. Myeloperoxidase staining did not differ between the groups, and scanning electron microscopy analysis showed similar characteristics in all groups. …”
Get full text

MSC-derived exosome ameliorates pulmonary fibrosis by modulating NOD 1/NLRP3-mediated epithelial-mesenchymal transition and inflammation by Wei Chen, Jie Peng, Xiangyi Tang, Shao Ouyang

“…Histopathological examination was performed using HE and Masson staining. Results: MSC-exos alleviated inflammation, inhibited epithelial-mesenchymal transition (EMT), and ameliorated PF. …”
Get full text

Feasibility and Safety of Pleuroscopic Cryobiopsy of the Pleura: A Prospective Study by Chia-Hung Chen, Wen-Chien Cheng, Biing-Ru Wu, Chih-Yu Chen, Wei-Chun Chen, Wei-Chih Liao, Chih-Yen Tu

“…The immunohistochemical (IHC) staining was more easily performed with CB (98.9%) compared to either FFB (87.0%) or CPB (13.0%). …”
Get full text

Effect of opercular deformity on morphological and transcriptomic profiling of gills in golden pompano (Trachinotus ovatus) by Yuwei Wu, Qian Ma, Dihao Huang, Liuyong Wang, Qiling Zhou, Chunli Ran, Gang Chen

“…Paraffin section, Hematoxylin-Eosin (HE) staining, and TdT-mediated dUTP nick-end labeling (TUNEL) of the gills revealed that no significant difference in morphological structure or apoptosis was identified between the gills collected from the deformed and normal sides. …”
Get full text

Predictive role of ABC transporters in the efficacy of enfortumab vedotin for urothelial carcinoma by Toshiki Kijima, Atsuko Takada‐Owada, Hiroki Shimoda, Hidetoshi Kokubun, Toshitaka Uematsu, Kohei Takei, Hironori Betsunoh, Masahiro Yashi, Kazuyuki Ishida, Takao Kamai

“…Immunohistochemical staining was performed on formalin‐fixed, paraffin‐embedded tissue samples. …”
Get full text

Discovery of two novel Flavobacterium species with potential for complex polysaccharide degradation by Xu-Dong Lian, Yong Guan, Yue Jiang, Dong-Heui Kwak, Mi-Kyung Lee, Zhun Li

“…These strains, which belong to the phylum Bacteroidota, are Gram-stain-negative, non-motile, aerobic, rod-shaped bacteria and have shown polysaccharide-degrading capabilities. …”
Get full text

Comparison of HER2-Targeted Antibodies for Fluorescence-Guided Surgery in Breast Cancer by Solmaz AghaAmiri, Jo Simien, Alastair M. Thompson, Julie Voss, Sukhen C. Ghosh, Servando Hernandez Vargas, Sarah Kim, Ali Azhdarinia, Hop S. Tran Cao

“…In vivo HER2 specificity was confirmed by immunohistochemical staining of resected tumors and normal tissues. Conclusions. …”
Get full text

A machine learning based algorithm accurately stages liver disease by quantification of arteries by Zhengxin Li, Xin Sun, Zhimin Zhao, Qiang Yang, Yayun Ren, Xiao Teng, Dean C. S. Tai, Ian R. Wanless, Jörn M. Schattenberg, Chenghai Liu

“…AD was counted using qVessel (previously trained on manually labeled vessels by stained slides (CD34/a-SMA/CK19) and developed by a decision tree algorithm). …”
Get full text

Targeted dual biologic therapy for erythroderma of unknown etiology guided by high-parameter peripheral blood immunophenotyping by Hannah L. Cornman, Martin P. Alphonse, Arbor Dykema, Alexander L. Kollhoff, Kevin K. Lee, Jaya Manjunath, Emily Z Ma, Varsha Parthasarathy, Junwen Deng, Thomas Pritchard, Anusha Kambala, Melika Marani, Kayla A. Parr, Javid P. Mohammed, Madan M. Kwatra, Jay H. Bream, Won Jin Ho, Shawn G. Kwatra

“…Whole-genome sequencing of PBMCs and immunofluorescence staining of skin biopsies revealed increased expression of Th2- (IL-13, IL-4Rα) and Th17-associated markers (IL-17, IL-17Rα) and non-functional mutations associated with Th2 and Th17 signaling, demonstrating that PBMCs can reflect cutaneous disease pathology. …”
Get full text

Jiaweishaoyao Decoction Alleviates DSS-Induced Ulcerative Colitis via Inhibiting Inflammation by Huixia Qiao, Yahui Huang, Xiaoyan Chen, Long Yang, Yue Wang, Rongrong Xu

“…UC was assessed by body weight, disease activity index (DAI), colon length, spleen weight, and histopathological score (HE staining). The levels of TNF-α, IL-1β, and IL-6 were analyzed by ELISA and qRT-PCR. …”
Get full text

Qufeng epimedium decoction alleviates rheumatoid arthritis through CYLD-antagonized NF-kB activation by deubiquitinating Sirt1 by Zhiming Wu, Peng Zhang, Wenyan Huang, Yifen Zhou, Zhengliu Cao, Chunhong Wu

“…Methods: The RA animal model was established to investigate the anti-RA effect and regulatory effect on fibroblast-like synoviocytes (FLS) pyroptosis, qRT-PCR, Western blot, flow cytometry, histology staining, and ELISA were utilized to confirm the gene and protein expressions. …”
Get full text

IL1RN and KRT13 Expression in Bladder Cancer: Association with Pathologic Characteristics and Smoking Status by Thomas S. Worst, Verena Reiner, Ute Gabriel, Christel Weiß, Philipp Erben, Thomas Martini, Christian Bolenz

“…Immunohistochemical staining of KRT13 and IL1RN was performed and semiquantitative expression scores were assessed. …”
Get full text