Metal ion-crosslinking multifunctional hydrogel microspheres with inflammatory immune regulation for cartilage regeneration by Zhuoming Xu, Zhuoming Xu, Jun Ma, Hanyin Hu, Hanyin Hu, Jintao Liu, Jintao Liu, Haiyang Yang, Haiyang Yang, Jiayi Chen, Hongwei Xu, Xinyu Wang, Huanhuan Luo, Gang Chen

“…In addition, in vivo MRI and tissue section staining of OA model proved the significant efficacy of the hydrogel microspheres on OA. …”
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Tissue Inhibitor of Matrix Metalloproteinases-1 Knockdown Suppresses the Proliferation of Human Adipose-Derived Stem Cells by Peihua Zhang, Jin Li, Yawei Qi, Xudong Tang, Jianfeng Duan, Li Liu, Zeyong Wu, Jie Liang, Jiangfeng Li, Xian Wang, Guofang Zeng, Hongwei Liu

“…Cell proliferation was then evaluated by MTT assay and Ki67 staining, respectively. Cell cycle progression was determined by flow cytometry. …”
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Structural Characterization of Functional Ingredient Levan Synthesized by Bacillus siamensis Isolated from Traditional Fermented Food in Thailand by Nattapong Thakham, Supphasin Thaweesak, Nuttinee Teerakulkittipong, Natthiwut Traiosot, Autaipohn Kaikaew, Gary Antonio Lirio, Witawat Jangiam

“…Isolated colonies were characterized morphologically by gram staining methods. Dinitrosalicylic acid (DNS) and thin-layer chromatography (TLC) reported the highest microbial enzyme activity of 8.51 IU/ml at 12 hours via hydrolysis and frutotransferase activities. …”
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A mouse model of deep vein thrombosis by inferior vena cava hypoperfusion using ameroid constrictors by Hiroko Tadokoro, Yukihide Ota, Mari Uomoto, Shiro Koizume, Shinya Sato, Yoshiyasu Nakamura, Mitsuyo Yoshihara, Yoko Endo-Takahashi, Yoichi Negishi, Etsuko Miyagi, Yohei Miyagi

“…The size of each thrombus was measured, and the composition was assessed using histological staining; BF through the IVC was confirmed using ultrasound imaging. …”
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DNA Damage and Augmented Oxidative Stress in Bone Marrow Mononuclear Cells from Angiotensin-Dependent Hypertensive Mice by Bianca P. Campagnaro, Clarissa L. Tonini, Breno V. Nogueira, Dulce E. Casarini, Elisardo C. Vasquez, Silvana S. Meyrelles

“…The current study was performed to examine the effects of angiotensin-II-dependent hypertension in bone marrow mononuclear cells (BM-MNC); dihydroethidium staining was used to assess reactive oxygen species (ROS) production, and the comet assay was used to assess DNA fragmentation in 2K1C hypertensive mice 14 days after renal artery clipping. …”
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Novel fusion protein REA induces robust prime protection against tuberculosis in mice by Sintayehu Kebede Gurmessa, Han-Gyu Choi, Yong Woo Back, Zongyou Jiang, Thuy An Pham, Seunga Choi, Hwa-Jung Kim

“…Notably, REA-immunized mice suppressed MTB growth to undetectable levels at 16 weeks post-infection, which was supported by gross and pathologic findings and acid-fast staining of the lung tissues, and maintained antigen-specific multifunctional IFN-γ+IL-2+TNF-α CD4+ T and long-lasting T cells producing cytokines in the tissues. …”
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Comparison of Lipid Content in Nine Dinoflagellate Species Using Flow Cytometry by Jaeyeon Park, Eun Young Yoon, Seung Joo Moon, Jun-Ho Hyung, Hangy Lee

“…Additionally, the correlation between lipid content, cell size, and carbon content in dinoflagellates was evaluated using BODIPY 505/515 staining. The flow cytometry side scatter (SSC) effectively represented relative cell size, showing a linear relationship with the equivalent spherical diameter (ESD). …”
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Collagen fibers quantification for liver fibrosis assessment using linear dichroism photoacoustic microscopy by Yang Qiu, Honghui Li, Kun Yu, Jiali Chen, Li Qi, Yinghua Zhao, Liming Nie

“…For validation purposes, collagen fibers were visualized with Sirius-red staining and quantitatively assessed through the collagen proportional area (CPA) score. …”
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The Sublingua of <i>Lemur catta</i> and <i>Varecia variegata</i>: Only a Cleaning Function? by Juan Francisco Pastor, Magdalena Natalia Muchlinski, Josep María Potau, Aroa Casado, Yolanda García-Mesa, José Antonio Vega, Roberto Cabo

“…To better understand the functional significance of the sublingua, we studied this structure in <i>Lemur catta</i> and <i>Varecia variegata</i> specimens using histochemical staining techniques and scanning electron microscopy with microanalysis. …”
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A 23-Year-Old Female with a Mixed Germ Cell Tumor of the Pituitary Infundibulum: The Challenge of Differentiating Neoplasm from Lymphocytic Infundibuloneurohypophysitis—A Case Repo... by Sann Yu Mon, Hussain Mahmud, Munira Abbasi, Geoff Murdoch, Juan C. Fernandez-Miranda, Paul A. Gardner, Sue M. Challinor

“…In our case, biopsy with comprehensive immunohistochemical staining was the sole means of making a definitive diagnosis.…”
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Metachronous Rectum Metastases from Gastric Adenocarcinoma: A Case Report by Deniz Tural, Fatih Selçukbiricik, Abdülkadir Erçalışkan, Berin İnanç, Feray Günver, Evin Büyükünal

“…Cytokeratin 7 (CK7) was found to be extensively strongly positive, Cytokeratin 20 (CK20) was negative in the immunohistochemical staining of the biopsy obtained from rectosigmoid area. …”
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Preliminary study on screening and selection of Lactic Acid Bacteria (LAB) from gastrointestinal tract of naleh fish (Barbonymus sp.) by Yulvizar Cut, Yunita Dewi, A Muchlizin Zainal, Suhartono Suhartono

“…The cell morphology is coccus - shaped (100%) and the bacteria are classified as Gram staining positive (100%). The growth of all LAB isolates from the gastrointestinal tract of the naleh fish is can survive by differences in temperature, NaCl concentration and catalase negative test.…”
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Assessment of the First Commercial ELISA Kit for the Diagnosis of Theileria annulata by Amira A. T. Al-Hosary, Jabbar Ahmed, Ann Nordengrahn, Malik Merza

“…Moreover, all samples were also analyzed by conventional Giemsa-stained blood smear. The results of this study revealed a good correlation between the results obtained by PCR and the ELISA, whereas all PCR positive samples scored correctly positive in the ELISA and 73 of the 125 PCR negative samples scored correctly negative. …”
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LIF Upregulates Expression of NK-1R in NHBE Cells by Cheng-Ping Hu, Jun-Tao Feng, Yu-Ling Tang, Jin-Qi Zhu, Min-Juan Lin, Ming-En Yu

“…In this study, the immunohistochemistry stain showed the expressions of NK-1R, LIF, p-STAT3, and p-ERK1/2 in the lung tissues of allergic rats were increased compared with the controls, and the main positive cell type was airway epithelial cell. …”
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Enhancing Enzyme-Mediated Cellulose Hydrolysis by Incorporating Acid Groups Onto the Lignin During Biomass Pretreatment by Jie Wu, Richard P. Chandra, Masatsugu Takada, Masatsugu Takada, Li-Yang Liu, Scott Renneckar, Kwang Ho Kim, Chang Soo Kim, Jack N. Saddler

“…Pretreated mechanical pulp (MP) made from Aspen wood chips was incubated with either 16% sodium sulfite or 32% sodium percarbonate to incorporate similar amounts of sulfonic and carboxylic acid groups onto the lignin (60 mmol/kg substrate) present in the pulp without resulting in significant delignification. When Simon’s stain was used to assess potential enzyme accessibility to the cellulose, it was apparent that both post-treatments enhanced accessibility and cellulose hydrolysis. …”
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Stathmin 1 expression in neuroendocrine and proliferating prostate cancer by Yingli Shi, Yunshin A. Yeh, Siyuan Cheng, Xin Gu, Shu Yang, Lin Li, Nazih P. Khater, Susan Kasper, Xiuping Yu

“…In this study, we investigated the expression of stathmin 1 (STMN1) across PCa subtypes using bioinformatics, western blotting, and immunohistochemical staining analyses in human and murine models. We found that elevated STMN1 expression correlated with high Gleason Scores, increased cell proliferation, and poor clinical outcomes in PCa patients. …”
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Photo-Induced Cell Damage Analysis for Single- and Multifocus Coherent Anti-Stokes Raman Scattering Microscopy by Takeo Minamikawa, Yoshinori Murakami, Naokazu Matsumura, Hirohiko Niioka, Shuichiro Fukushima, Tsutomu Araki, Mamoru Hashimoto

“…For the evaluation of cell viability, we employed 4′,6-diamidino-2-phenylindole (DAPI) fluorophores that predominantly stained the damaged cells. One- and two-photon fluorescence of DAPI fluorophores were, respectively, excited by an ultraviolet light source and the same near-infrared light source and were monitored to evaluate the cell viability during near-infrared pulsed laser irradiation. …”
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Transmission Electron Microscopic Morphological Study and Flow Cytometric Viability Assessment of Acinetobacter baumannii Susceptible to Musca domestica cecropin by Shuiqing Gui, Rongjiang Li, Yongwen Feng, Sanming Wang

“…Flow cytometry using viability stain demonstrated that Mdc causes A. baumannii membrane permeabilization in a concentration- and time-dependent process, which correlates with the bactericidal action. …”
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Observation of Blood Donor-Recipient Malaria Parasitaemia Patterns in a Malaria Endemic Region by Jamilu Abdullahi Faruk, Gboye Olufemi Ogunrinde, Aisha Indo Mamman

“…Blood donor units and patients’ blood samples were obtained, for the determination of malaria parasites (MPs). Giemsa staining technique was used to determine the presence of malaria parasitaemia. …”
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Electroacupuncture regulates ferroptosis to improve postoperative cognitive dysfunction in mice through mediating GRX1/GSK-3β/Nrf2 axis by Xiaqing Zhang, Afen Zhang, Rui Li, Mingna Jiao, Rou Wen, Rongzhi Zheng

“…Morris water maze (MWM) was applied to evaluate cognitive impairment of mice. HE staining was used to examine cell arrangement and cell morphology in hippocampus of mice. …”
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