Antihepatitis B Virus Activity of a Protein-Enriched Fraction from Housefly (Musca domestica) in a Stable HBV-Producing Cell Line by Xuemei Lu, Xiaobao Jin, Jie Wang, Fujiang Chu, Jiayong Zhu

“…HBV core protein was assayed by immunofluorescent staining. Results indicate PE treatment inhibited both HBsAg, HBeAg secretion, and HBV-DNA replication. …”
Get full text

Identification of Ectoparasites and Gastrointestinal Tract Endoparasites in Stray Cats at Traditional Market of Bojonegoro City by Karina Rachmawati, Setiawan Koesdarto, Iwan Sahrial Hamid, Dian Ayu Permatasari

“…The ectoparasite samples were examined using the permanent attachment method without staining, while the feces samples were examined using three methods, namely native, simple sedimentation and fulleborn floatation. …”
Get full text

Quantification and identification of bacterial presence in salted eggs by Zukhrufa Vista Vindriati, Usamah Afiff, Trioso Purnawarman

“…The total bacterial count was determined using the plate count agar method, and bacterial identification was based on phenotypic analysis, which included Gram staining and biochemical tests. Results The total bacterial load in fresh salted eggs was below the maximum limit set by SNI 7388:2009 (1×105 CFU/g), whereas the pasta dough and black egg yolk exceeded this limit. …”
Get full text

Meningococcal Neonatal Purulent Conjunctivitis/Sepsis and Asymptomatic Carriage of N. meningitidis in Mother’s Vagina and Both Parents’ Nasopharynx by Enrique Chacon-Cruz, Jorge Arturo Alvelais-Palacios, Jaime Alfonso Rodriguez-Valencia, Erika Zoe Lopatynsky-Reyes, Maria Luisa Volker-Soberanes, Rosa Maria Rivas-Landeros

“…Clinical examination confirmed profuse purulent conjunctival discharge, as well as clinical signs and laboratory findings suggestive of bacteraemia. Gram stain from conjunctival exudate revealed intracellular Gram negative diplococci; we presumed the baby had gonorrheal conjunctivitis; however, serogroup Y, N. meningitidis was isolated both from conjunctival exudate and blood. …”
Get full text

A Technique for Section Thickness Evaluation for Microphotometry and Image Analysis of Sectioned Nuclei by R. L. Cabrini, A. Folco, S. Orrea, M. T. Savino, A. M. Schwint, M. E. Itoiz

“…The standards consist of celloidin cylinders stained with the PAS reaction and embedded in paraffin. …”
Get full text

Diagnosing Cardiac Amyloidosis: From Heart Failure to Electrical Storm by Adithya T. Mathews, Abu-Sayeef Mirza, Chandrashekar Bohra, Akshay G. Mathews, Philip Ritucci-Chinni, Jamie L. Weber, William W. Bulkeley, Maqsood Siddique, David Whitaker, Richard Bowerman

“…Autopsy revealed gross cardiomegaly and left ventricular hypertrophy with Congo red staining revealing amyloid fibrils with apple-green birefringence. …”
Get full text

Expression of Semaphorin 3F and Its Receptors in Epithelial Ovarian Cancer, Fallopian Tubes, and Secondary Müllerian Tissues by Christina D. Drenberg, Sandra Livingston, Ren Chen, Patricia A. Kruk, Santo V. Nicosia

“…Tissue sections were evaluated for staining intensity and percentage of immunoreactive epithelia. …”
Get full text

Purification and Characterization of a Novel Intracellular Sucrase Enzyme of Leishmania donovani Promastigotes by Arpita Singh, Debjani Mandal

“…The estimated size of intracellular sucrase is ~112 kDa by gel filtration chromatography, native PAGE, and substrate staining. However, in SDS-PAGE, the protein is resolved at ~56 kDa, indicating the possibility of a homodimer in its native state. …”
Get full text

Iodine test to detect physical damage in chickpea seeds by Jose de Oliveira Cruz, Luís Carlos de Carvalho Júnior, Ricardo Carmona, Jônatas Barros dos Santos, Isabella Fuckner Artiaga

“…With the aim of developing a protocol for rapid iodine testing, the aim of this study was to determine the most suitable exposure time for chickpea seeds in iodine solution, and the most appropriate concentration for staining naturally or artificially damaged seeds. Concentrations of 5%, 10% and 15% (commercial product containing 2% iodine) were tested on both intact and artificially damaged seeds for an immersion time of 2.5, 5.0, 10.0, 15.0 and 20.0 minutes. …”
Get full text

Resveratrol-loaded PCL-PEG/GO/HAP biocomposite bone membranes: Evaluation of mechanical properties, release kinetics, and cellular response by Betül Meryem Arpacay, Fatih Ciftci, Ali Can Özarslan, Mustafa Unal, Mine Kucak, Aslihan Yelkenci

“…In vitro cell adhesion studies, visualized using DAPI fluorescence staining, demonstrated increased cell adhesion to the composite membranes over periods of 1, 3, 5, 7, and 14 days. …”
Get full text

Solitary Fibrous Tumor of Neck Mimicking Cold Thyroid Nodule in 99m Tc Thyroid Scintigraphy by Oya Topaloglu, Bekir Ucan, Taner Demirci, Muyesser Sayki Arslan, Guleser Saylam, Evrim Onder, Sinan Gultekin, Alper Dilli, Mustafa Sahin, Erman Cakal, Mustafa Ozbek, Tuncay Delibasi

“…Immunohistochemistry showed positive reaction for CD34 in spindle cells and diffuse bcl-2 staining. The pathology was confirmed as solitary fibrous tumor. …”
Get full text

Prevalence of Rumen and Reticulum Trematodes in The Goat Slaughtered at Pegirian Surabaya Slaughter House using Digestive Surgery Method by Novia Intan Kurnia, Setiawan Koesdarto, Herry Agoes Hermadi, Kusnoto Kusnoto, Hardany Primarizky, Agus Sunarso

“…The purpose of this study was to determine the kind of trematodes which infected the rumen and reticulum of goat slaughtered at RPH Pegirian Surabaya using digestive surgery method, and determine the prevalence result. 36 samples of rumen and reticulum were collected from RPH Pegirian from September - November 2017 then using digestive surgery to obtain fresh adult worms, afterwards using the Semichen-Acetic Carmine staining method to makes the object easy to identifying. …”
Get full text

The Dynamic DNA Demethylation during Postnatal Neuronal Development and Neural Stem Cell Differentiation by Huikang Tao, Pei Xie, Yuhang Cao, Liqi Shu, Liping Li, Junchen Chen, Guangfeng Tian, Yingliang Zhuang, Qiang Shu, Xuekun Li

“…In this study, utilizing DNA dot blot, immunofluorescence staining, and qRT-PCR, we studied the expression pattern of Tets, the enzymes governing DNA demethylation, and the levels of 5hmC, 5fC, and 5caC during the postnatal neuronal development of mice. …”
Get full text

Detection of Mechanically Recovered Poultry Meat (MRPM) in Traditional Egyptian Luncheon (Emulsion Type Sausage) by Mohamed Mai A., Zahran Dalia A., Kassem Gehan M.A., Emara M.M.T., Mansour N.M.

“…Commercial and experimentally produced emulsion type sausage (traditional Egyptian luncheon) formulated with 0, 10, 30, 50, 70, 90% MRPM instead of the meat mass and cooked to different core temperature (70, 80 and 90°C) were examined for technological properties, ash, bones, cartilage and calcium (Ca) content, in addition to histological sections stained with H&E and Trichrome blue. Results indicated that all market samples showed unacceptable texture and binding scores with high ash, collagen, cartilage, bone and Ca content. …”
Get full text

Detecting Oil Slicks Using SAR Radar Images and the Google Earth Engine Platform by Ali Kourosh Niya, Ali Ashrafi

“…Comparing the obtained results with oil pollution events in the study area shows the appropriate efficiency of the proposed framework for detecting oil stains.…”
Get full text

GRHL3 Promotes Tumor Growth and Metastasis via the MEK Pathway in Colorectal Cancer by Lin Tan, Weiming Qu, Dajun Wu, Minji Liu, Qian Wang, Qiongjia Ai, Hongsai Hu, Min Chen, Weishun Chen, Hongbing Zhou

“…The expression of GRHL3 was measured in CRC and adjacent normal tissue using qPCR and immunohistochemical staining. Lentivirus-mediated knockdown expression of GRHL3 was performed in the CRC cell line HT29. …”
Get full text

Tuberculous Empyema Necessitatis in a 40-Year-Old Immunocompetent Male by Farhang Babamahmoodi, Lotfollah Davoodi, Roya Sheikholeslami, Fatemeh Ahangarkani

“…Smear and Ziehl-Neelsen stains from subcutaneous aspiration were positive for TB. …”
Get full text

Randomised Comparison of the AMBU AuraOnce Laryngeal Mask and the LMA Unique Laryngeal Mask Airway in Spontaneously Breathing Adults by Daryl Lindsay Williams, James M. Zeng, Karl D. Alexander, David T. Andrews

“…There was no statistical difference between the two devices for overall success rate, insertion time, number of adjustments, laryngeal alignment, blood-staining, and sore throat (P≥0.05). The AMBU AuraOnce disposable laryngeal mask provided a higher oropharyngeal leak pressure compared to the LMA Unique in spontaneously breathing adult patients.…”
Get full text

Ovarian Seromucinous Borderline Tumor and Clear Cell Carcinoma: An Unusual Combination by Eriko Nakamura, Yuichiro Sato, Sayaka Moriguchi, Atsushi Yamashita, Takashi Higo, Yujiro Asada

“…Immunohistochemistry showed different staining patterns in tumor components. Seromucinous tumor cells were positive for estrogen receptor (ER) and progesterone receptor (PgR) but negative for Napsin A, p504S, and HNF1B. …”
Get full text

Morphological Changes in the Bone Marrow of the Dogs with Visceral Leishmaniasis by Claudia Momo, Ana Paula Prudente Jacintho, Pamela Rodrigues Reina Moreira, Danísio Prado Munari, Gisele Fabrino Machado, Rosemeri de Oliveira Vasconcelos

“…At the necropsy, bone marrow samples were collected from the femur, fixed, processed, and stained with hematoxylin and eosin. The lesion intensity was classified as mild, moderate, or severe. …”
Get full text