Induced Pluripotent Stem Cells Reduce Progression of Experimental Chronic Kidney Disease but Develop Wilms’ Tumors by Heloisa Cristina Caldas, Fernando Henrique Lojudice, Cinthia Dias, Ida Maria Maximina Fernandes-Charpiot, Maria Alice Sperto Ferreira Baptista, Rosa Sayoko Kawasaki-Oyama, Mari Cleide Sogayar, Christina Maeda Takiya, Mario Abbud-Filho

“…These 5 animals presented tumors with histology and cells highly staining positive for PCNA and Wilms’ tumor protein antibody characteristics of Wilms’ tumor. …”
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Comparative Genomic Hybridization (CGH) in New World Monkeys (Primates) Reveals the Distribution of Repetitive Sequences in Cebinae and Callitrichinae by Vanessa Milioto, Vincenzo Arizza, Aiti Vizzini, Polina L. Perelman, Melody E. Roelke-Parker, Francesca Dumas

“…The distribution of repetitive sequences has been traditionally studied through classical staining methods of cytogenetics. It has been hypothesized that repeats are species-specific and their conservation across closely related species are also common; their role in the genome has been extensively studied even though its role in speciation is not well studied and understood. …”
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Influence of the Cement Film Thickness on the Push-Out Bond Strength of Glass Fiber Posts Cemented in Human Root Canals by Natália Araújo Silva Prado, Reinaldo de Souza Ferreira, Marcos Henrique de Pinho Maurício, Sidnei Paciornik, Mauro Sayão de Miranda

“…The posts were cemented using a self-adhesive cement, and a small amount of powdered Rhodamine B was used as a stain. Images of both sides of each slice were obtained before and after the push-out test. …”
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HMGB1 Promotes Systemic Lupus Erythematosus by Enhancing Macrophage Inflammatory Response by Mudan Lu, Shanshan Yu, Wei Xu, Bo Gao, Sidong Xiong

“…Furthermore, nephritic pathology was evaluated by H&E staining of renal tissues. Results. In this study, we found that HMGB1 levels were significantly increased and correlated with SLE disease activity in both clinical patients and murine model. …”
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Prevalence of Bacterial Vaginosis and Associated Risk Factors among Women Complaining of Genital Tract Infection by Adane Bitew, Yeshiwork Abebaw, Delayehu Bekele, Amete Mihret

“…Paul’s Hospital. Gram-stained vaginal swabs were examined microscopically and graded as per Nugent’s procedure. …”
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Atypical Radiographic Presentation of Cryptococcus Pneumonia in a Newly Diagnosed HIV Patient by Arthur Cacacho, Umair Ashraf, Arsalan Rehmani, Masooma Niazi, Misbahuddin Khaja

“…Interferon-gamma release assay, Mycobacterium tuberculosis PCR, and acid-fast bacilli staining of the bronchoalveolar lavage were negative for the M. tuberculosis complex. …”
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Pre-screening of endomysial microvessel density by fast random forest image processing machine learning algorithm accelerates recognition of a modified vascular network in idiopath... by Alessandro Massaro, Gerardo Cazzato, Giuseppe Ingravallo, Nadia Casatta, Carmelo Lupo, Angelo Vacca, Florenzo Iannone, Francesco Girolamo

“…The FRF technique applied on images of CD31-stained muscle sections achieved a good performance in the recognition of microvessels by estimating their density over a standard area corresponding to a sample of microscope image. …”
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The quantitative characteristic of morphological and structural changes in the diverted regions of the large intestine by A. S. Akopyan, E. V. Manukyan, T. G. Bagdasaryan, A. F. Abramyan, A. A. Akopyan

“…Morphological investigation was carried out under the practical standard with staining of samples by hematoxylin and eosine. Stereometric analysis principles were used. …”
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A comparative study of mitoxantrone hydrochloride injection and technetium-99 m for tracing axillary sentinel nodes in patients with breast cancer by Dechuang Jiao, Xiuchun Chen, Xianfu Sun, Zhenduo Lu, Hao Dai, Chengzheng Wang, Chongjian Zhang, Lianfang Li, Jianghua Qiao, Zhenzhen Liu

“…The SLN detection rate did not significantly differ between MHI and 99mTc. MHI blue staining was an independent predictor of pathological positivity (OR 3.58, 95% CI: 1.21–10.60, p = 0.02), while the 99mTc signal was not significant in the same model (p = 0.39). …”
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Caffeic Acid Phenethyl Ester Regulates PPAR’s Levels in Stem Cells-Derived Adipocytes by Luca Vanella, Daniele Tibullo, Justyna Godos, Francesca Romana Pluchinotta, Claudia Di Giacomo, Valeria Sorrenti, Rosaria Acquaviva, Alessandra Russo, Giovanni Li Volti, Ignazio Barbagallo

“…Quantification of Oil Red O-stained cells showed that lipid droplets decreased following Cape treatment as well as radical oxygen species formation. …”
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Ginsenoside Rg1 Improves Differentiation by Inhibiting Senescence of Human Bone Marrow Mesenchymal Stem Cell via GSK-3β and β-Catenin by Ziling Wang, Rong Jiang, Lu Wang, Xiongbin Chen, Yue Xiang, Linbo Chen, Minghe Xiao, Li Ling, Yaping Wang

“…The differentiation ability of hBM-MSCs was studied by differentiation culture. SA-β-gal staining is used to detect cell senescence levels. …”
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Cytotoxic and Apoptotic Effects of Different Extracts of Artemisia turanica Krasch. on K562 and HL-60 Cell Lines by Zahra Tayarani-Najaran, Mahla Sareban, Atefeh Gholami, Seyed Ahmad Emami, Mahdi Mojarrab

“…In this study, we have investigated the cytotoxic effects of n-hexane, CH2Cl2, EtOAc, EtOH, and EtOH/H2O (1 : 1) extracts of A. turanica Krasch. on two human leukemic cancer cell lines (K562 and HL-60) and J774 as normal cells using alamarBlue (resazurin) assay. PI staining of the fragmented DNA and western blot analysis were used to evaluate the possible apoptotic effect of the extract. …”
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The Influence of Mg2+ Ions on the In Vitro Efficacy of Chitosan-Titanium Dioxide Nanotubes (CTNTs) Scaffolds by Siew Shee Lim, Shui Wei Kho, Nehemiah Li Heng Ang, Cheng Heng Pang, Hwei-San Loh

“…Its adsorption isotherm fitted well with Langmuir isotherm by showing R2 of 0.9995. Fluorescence-based staining, cell viability, and alkaline phosphatase assays indicated that the adsorbed Mg2+ ions onto CTNTs scaffolds aided in promoting higher proliferation and early differentiation of MG63 cells than scaffolds without Mg2+ ions in a concentration-dependent manner. …”
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Human Amniotic Fluid Mesenchymal Stem Cells from Second- and Third-Trimester Amniocentesis: Differentiation Potential, Molecular Signature, and Proteome Analysis by Jurate Savickiene, Grazina Treigyte, Sandra Baronaite, Giedre Valiuliene, Algirdas Kaupinis, Mindaugas Valius, Audrone Arlauskiene, Ruta Navakauskiene

“…AF-MSCs differentiated into adipocytes, osteocytes, chondrocytes, myocytes, and neuronal cells, as determined by morphological changes, cell staining, and RT-qPCR showing the tissue-specific gene presence for differentiated cell lineages. …”
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Immunohistological Localization of BMP-2, BMP-7, and Their Receptors in Knee Joints with Focal Cartilage Lesions by Hagen Schmal, Alexander T. Mehlhorn, Ingo H. Pilz, David Dovi-Akue, Christina Kirchhoff, Norbert P. Südkamp, Ulrike Gerlach, Christian Lohrmann, Philipp Niemeyer

“…Expression of BMP-2, BMP-7, and their receptors BMPR-1A, BMPR-1B and BMPR-2 were semiquantitatively evaluated by immunohistological staining. Results. BMP-7 was equally highly expressed in all cartilage and synovial biopsies. …”
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Tumor-associated neutrophils and macrophages interaction contributes to intrahepatic cholangiocarcinoma progression by activating STAT3 by Jian Zhou, Jia Fan, Zhiqiang Hu, Jia Li, Zhengjun Zhou, Pengcheng Wang, Rongqi Sun, Haoyang Xin, Chubin Luo, Shaolai Zhou

“…Background Tumor-associated neutrophils (TANs) and macrophages (TAMs) can each influence cancer growth and metastasis, but their combined effects in intrahepatic cholangiocarcinoma (ICC) remain unclear.Methods We explored the distributions of TANs and TAMs in patient-derived ICC samples by multiplex immunofluorescent staining and tested their separate and combined effects on ICC in vitro and in vivo. …”
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Matrix Metalloproteinase-9 is associated with tumor microenvironment remodeling of bladder cancer by Fang Fang, Tiange Wu, Mengxue Wang, Wenchao Li, Zonghao You, Ming Chen, Han Guan

“…The results were confirmed by brightfield and multiplex fluorescence immunohistochemistry using stained bladder cancer and normal tissue.…”
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Clinical Feature of Intrahepatic B-Lymphocytes in Chronic Hepatitis B by Ashraf Mohamadkhani, Elnaz Naderi, Masoud Sotoudeh, Aezam Katoonizadeh, Ghodratollah Montazeri, Hossein Poustchi

“…The CD20 was expressed in all 57 liver biopsy samples with a submembranous and membranous staining pattern and its expression was significantly associated with HAI total score (r=0.32, P=0.01) and stage of fibrosis (r=0.31, P=0.02). …”
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Low CD4/CD8 Ratio in Bronchus-Associated Lymphoid Tissue Is Associated with Lung Allograft Rejection by K. V. Shenoy, C. Solomides, F. Cordova, T. J. Rogers, D. Ciccolella, G. J. Criner

“…BALT if present was immunohistochemically stained to determine T-and B-cell subpopulations. …”
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Neuroprotective Effects of Bone Marrow Mesenchymal Stem Cells on Bilateral Common Carotid Arteries Occlusion Model of Cerebral Ischemia in Rat by Bagher Pourheydar, Sara Soleimani Asl, Mostafa Azimzadeh, Adel Rezaei Moghadam, Asghar Marzban, Mehdi Mehdizadeh

“…We performed neuromuscular and vestibulomotor function tests to assess behavioral function and, finally, brains were subjected to hematoxylin and eosin (H&E), anti-Brdu immunohistochemistry, and TUNEL staining. The ischemia group had severe apoptosis. …”
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