Identification and Development of Synovial B-Cell-Related Genes Diagnostic Signature for Rheumatoid Arthritis by Jifeng Tang, Jinfang Xia, Huiming Sheng, Jinpiao Lin

“…Single-sample gene set enrichment analysis (ssGSEA) was performed to evaluate the abundance of infiltrated immune cells, and the results were validated using immunohistochemical staining. GSEA was employed to decipher differences in B-cell-related biological pathways. …”
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miR-224-5p Attenuates Allergic Responses in Mice with Allergic Rhinitis by Modulating the Th1/Th2 Response by Yuxiao Li, Ran An, Mingjin Wu, Jiayan He, Xiaoguang He

“…RT-qPCR was used to determine the level of miR-224-5p; western blot analysis was used to determine GATA3; ELISA was used to determine the levels of OVA-specific IgE, IFN-γ, IL-4, IL-5, and IL-13; flow cytometry was used to determine the differentiation of Th1 and Th2 cells; and HE and PAS staining was used to observe the histopathological alterations in the mouse nasal mucosa and spleen. …”
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Estimation of Indole-3-Acetic Acid Production from New Molecularly Characterized Rhizobacterial Strains by Mohammed Ayad Harbawi, Najwa Ibrahim Khaleel Al-Barhawee

“…All isolates were bacilliform, with clear differences in their response to Gram staining and biochemical tests. On the other hand, the results showed that root colonizing bacteria could produce different amounts of indole-3-acetic acid (IAA). …”
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Effect of Codonopsis pilosula Polysaccharides on the Growth and Motility of Hepatocellular Carcinoma HepG2 Cells by Regulating β-Catenin/TCF4 Pathway by Yuan-yuan Zhang, Ying-mei Zhang, Hai-yan Xu

“…The proliferation, invasion, migration ability, and expression of proteins involved in the epithelial-mesenchymal transition (EMT) and signaling pathway of HepG2 cells were detected by CCK8 assay, BrdU staining, Transwell, Scratch test, and Western blot, respectively. …”
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Poorly differentiated biphasic synovial sarcoma of the vulva, displaying SS18::SSX1 fusion and weak to absent (mosaic) INI1/SMARCB1 immunostaining: A rare case with literature revi... by Bharat Rekhi, Sheenal Bhatia, Omshree Shetty, Amita Maheshwari

“…INI1/SMARCB1 showed a characteristic weak to absent (mosaic) staining pattern. Furthermore, the tumor displayed SS18::SSX1 fusion by RT-PCR. …”
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Toll-like receptor 2/6-stimulated HMC-1 mast cells promote keratinocyte migration in wound healing. by Jiyun Kwon, Kyung-Ah Cho, So-Youn Woo

“…Wound closure was measured, and tissue regeneration was assessed using hematoxylin and eosin staining. Growth factor expression levels were evaluated to identify the factors affecting wound healing. …”
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Recursive seed amplification detects distinct α-synuclein strains in cerebrospinal fluid of patients with Parkinson’s disease by Stefan Bräuer, Iñaki Schniewind, Elisabeth Dinter, Björn H. Falkenburger

“…In addition, cultured cells were transfected with SAA products, and the extent of seeded aSyn pathology was quantified by staining for phosphorylated aSyn followed by automated high-throughput microscopy and image analysis. …”
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Atypical Antiglomerular Basement Membrane Disease in a Pregnant Patient with Systemic Lupus Erythematosus by Areerat Ounhasuttiyanon, Ngoentra Tantranont, Thatsaphan Srithongkul

“…A kidney biopsy showed linear capillary wall staining with fibrous crescents without endocapillary proliferation. …”
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Bone Formation from Porcine Dental Germ Stem Cells on Surface Modified Polybutylene Succinate Scaffolds by Nergis Abay, Gorke Gurel Pekozer, Mustafa Ramazanoglu, Gamze Torun Kose

“…Besides, alkaline phosphatase (ALP) assay, von Kossa staining, real-time PCR, CM-Dil, and immunostaining were applied to analyze osteogenic potentials of pDGSCs. …”
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Prevalence of Intestinal Protozoan Parasites and Associated Risk Factors among School Children in Merhabete District, Central Ethiopia by Nigus Dagne, Amir Alelign

“…Stool samples were collected and examined using direct wet mount, Formal-Ether concentration and Modified Zeihel-Neelsen staining techniques. Results. The overall prevalence of intestinal protozoan parasitic infection was found to be 46.8% (131/280). …”
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Remimazolam Combined with Andrographolide Improve Postoperative Cognitive Dysfunction in Rats after Cardiopulmonary Bypass through the AMPK/SIRT1 Signaling Pathway by Chong Chen, Lixia Lan, Kai Xu

“…Apoptosis was evaluated using western blotting and the terminal deoxynucleotidyl transferase (TdT)-mediated dUTP nick end labeling (TUNEL) staining assay. …”
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Activation of TLR4-Mediated NF𝜅B Signaling in Hemorrhagic Brain in Rats by Weiyu Teng, Lishu Wang, Weishuang Xue, Chao Guan

“…TLR4 mRNA and protein, the phosphorylation of inhibitors of kappa B (p-I𝜅B𝛼), and the activity of NF𝜅B were examined in hemorrhagic cerebral tissue by Rt-PCR, Western blots, immunohistochemistry staining, and EMSA. Compared with saline control, the TLR4 mRNA and protein significantly increased starting at 6 hours after ICH, peaked on the 3rd day after ICH, and then decreased but still maintained at a higher level on the 7th day after ICH (𝑃<.05). …”
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Efficacy of SG Shield in reducing droplet contamination during collection of oropharyngeal swab culture specimens by Phui-Sze Angie Au-Yong, Xuanxuan Chen, Wen Hao Low, Keen Chong Chau, Stephanie Fook-Chong, Shariq Ali Khan

“…The primary outcome was droplet dispersion, determined quantitatively by calculating the proportion of grid paper wall squares stained with fluorescent dye. The secondary outcome was the severity of provider contamination. …”
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Chemical Composition and Microbial Contaminants of Poha Beer: A Local Nonalcoholic Beverage in the Bolgatanga Municipality, Ghana by Julius T. Dongdem, James Abugri, Clement A. Asakedola

“…Morphological examination of bacterial cultures, Gram staining, and biochemical confirmatory tests were used to detect the presence of microbial pathogens in 45 samples of Poha Beer. …”
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Investigation of nosema apis and nosema ceranae in bees by multiplex pcr in kırıkkale region by Meral Aydenizoz, Ali Can Yorulmaz

“…The samples with Nosema spores were stained with Saffron and the multiplex PCR was applied to detect specimens to the same samples.…”
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San-Huang-Yi-Shen Capsule Ameliorates Diabetic Kidney Disease through Inducing PINK1/Parkin-Mediated Mitophagy and Inhibiting the Activation of NLRP3 Signaling Pathway by Hanzhou Li, Yuansong Wang, Xiuhai Su, Qinghai Wang, Shufang Zhang, Wenjuan Sun, Tianyu Zhang, Mengxue Dong, Zhaiyi Zhang, Shuquan Lv

“…In addition, western blot and immunoflourescence staining showed that SHYS treatment induced the PINK1/Parkin-mediated mitophagy and inhibited the activation of NLRP3 signaling pathway. …”
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Computer-tomography features of hemodynamics in hepatocellular carcinoma foci depend on the stage of histological differentiation by U. N. Tumanova, N. I. Yashina, Ye. A. Dubova, G. G. Karmazanovsky, A. I. Schegolev

“…The morphometric analysis of HCC blood vessels was carried out in immunehistochemical specimens stained for CD34 and CD105.Results. According to CT and immunohistochemistry data highly differentiated carcinomas were the most vascularized (hypervascular), while poorly differentiated HCC were the least vascularized. …”
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Groping for Quantitative Digital 3-D Image Analysis: An Approach to Quantitative Fluorescence In Situ Hybridization in Thick Tissue Sections of Prostate Carcinoma by Karsten Rodenacker, Michaela Aubele, Peter Hutzler, P. S. Umesh Adiga

“…Confocal laser scanning microscopy is the means to obtain series of optical thin sections from fluorescence stained or marked material to fulfill the conditions mentioned above. …”
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Er-Xian Decoction Stimulates Osteoblastic Differentiation of Bone Mesenchymal Stem Cells in Ovariectomized Mice and Its Gene Profile Analysis by Shufen Liu, Jianhua Huang, Jing Wang, Yongjian Zhao, Sheng Lu, Yongjun Wang, Qin Bian

“…The self-renewal and differentiation capacities of bMSCs were evaluated by colony-forming unit-fibroblastic, colony-forming unit-adipocyte, and alkaline phosphatase staining. In addition, the expression of 26991 genes of bMSCs ex vivo at 2 weeks after EXD-treatment or of bMSCs in vitro after exposure to conditioned serum from EXD-treated rats was measured and analyzed using NimbleGen Gene Expression Profiling and Cluster and pathway analysis. …”
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Oxidative Stress in Hypertensive Patients Induces an Increased Contractility in Vein Grafts Independent of Endothelial Function by Claudio Joo Turoni, Rodrigo Marañón, Maria Karbiner, Juan Muntaner, Víctor Proto, María Peral de Bruno

“…Antioxidant agents increased nitrites and NO contents only in HT. The anti-nNOS-stained area by immunohistochemistry was higher in NT than HT. …”
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