Effect of supplementing epinephrine in maturation media on the developmental competence of buffalo oocytes by Ali Husnain, Abdul Khaliq, Talha Ashraf, Abdul Rehman, Muhammad Yasir Zahoor, Muhammad Binyameen, Amjad Riaz

“…The maturation of oocytes was examined based on the nuclear maturation after staining with Hoechst 33342 (Sigma Aldrich, St. Louis, MO, USA). …”
Get full text

Electroacupuncture Regulates Disorders of Gut-Brain Interaction by Decreasing Corticotropin-Releasing Factor in a Rat Model of IBS by Ying Chen, Yan Zhao, Dan-ni Luo, Hui Zheng, Ying Li, Si-yuan Zhou

“…Compared with the blank control group, visceral pain threshold pressure, the expression of ZO-1, OT%, SP%, CRF, and CRF-R1 mRNA expression in the hypothalamus, and double staining of IMMC and CRF-R1 were decreased significantly in the CUMS group. …”
Get full text

CEST imaging combined with 1H-MRS reveal the neuroprotective effects of riluzole by improving neurotransmitter imbalances in Alzheimer’s disease mice by Yuanyu Shen, Xiaolei Zhang, Siqi Liu, Lijing Xin, Wentao Xuan, Caiyu Zhuang, Yue Chen, Beibei Chen, Xinhui Zheng, Renhua Wu, Yan Lin

“…At the study endpoint, immunohistochemistry, Nissl staining, and Western blot were used to evaluate the brain pathology, neuronal survival, and protein expression. …”
Get full text

Effects of Duhuo Jisheng Decoction on Calcium Homeostasis and Apoptosis of Chondrocytes under Endoplasmic Reticulum Stress by Qing LIN, Wenyi WANG, Danhong PAN, Yanfeng HUANG, Changlong FU, Jinxia YE

“…Objective:To observe the effect of Duhuo Jisheng decoction on the balance of calcium homeostasis and apoptosis of chondrocytes under endoplasmic reticulum stress(ERS), and to preliminarily explore its mechanism of preventing and treating osteoarthritis.Methods:A steady chondrocyte in vitro culture system was established by using 4-week-old SD rat knee cartilage, and identify the third generation chondrocyte by toluidine blue staining.The 25 μmol/L Thapsigargin(TG)was used to induce chondrocyte in vitro culture to produce endoplasmic reticulum stress(ERS), incubate it in Duhuo Jisheng Decoction(100, 200, 400 μg/mL)for 24 hours after induction.The cells were divided into the blank group, the model group, the low-dose Duhuo Jisheng decoction group, the medium-dose Duhuo Jisheng decoction group, and the high-dose Duhuo Jisheng decoction group.Then morphological changes of chondrocytes were observed respectively by inverted microscope, viability of chondrocyte in each group was examined by CCK-8 method, chondrocyte apoptosis was observed by Hoechst 33342 staining and real-time dynamic changes of intracellular Ca²⁺concentration was examined by double turntable laser confocal microscope.Results:①The morphology of chondrocytes: the cells in the blank group grew in a single-layer paving stone shape, while the cells in the model group increased in a polygonal shape, the pseudopodia increased, the gaps widened, and the number of cells decreased significantly.Compared with the model group, the number of cells in the three dose groups of Duhuo Jisheng decoction(100, 200, 400 μg/mL)increased significantly and the cytoplasm was uniform.The activity of chondrocytes in each group: compared with the blank group, the survival rate of the model group was lower, and the difference was statistically significant(<italic>P</italic>&lt;0.05).The absorbance of each concentration group of Duhuo Jisheng decoction increased with the increase of concentration, and the difference was statistically significant compared with the model group(<italic>P</italic>&lt;0.05).③Apoptosis of chondrocytes: the number of apoptosis in the model group was significantly increased, and the degree of pyknosis of the nucleus was obvious, and some of them were a high density of bright blue or half moon-shaped.The number of apoptotic cells in each dose group of Duhuo Jisheng decoction was significantly reduced, only a few apoptotic cells showed a high density of bright blue or half-moon shape, and the number of apoptosis decreased with the increase of the concentration of Duhuo Jisheng decoction, showing a certain dose-effect relationship.④The changes of intracellular calcium ion concentration: the calcium ion fluorescence intensity of the cells in the blank group was weaker and more uniform, maintaining a stable state.The calcium ion release of the model group was significantly increased, and the fluorescence intensity was enhanced.The fluorescence spectrum showed that calcium sparks were more frequent.Compared with the blank group, the difference was statistically significant(<italic>P</italic>&lt;0.05).Chondrocytes in the medium and high-dose Duhuo Jisheng decoction groups could quickly return to calcium homeostasis, and calcium sparks were significantly reduced.Although there were still some calcium sparks in the low-concentration Duhuo Jisheng decoction group, the fluorescence intensity was significantly weaker than the model group.Conclusion:Duhuo Jisheng decoction has an inhibitory effect on the apoptosis of chondrocyte induced by TG, the mechanism may be that Duhuo Jisheng decoction can alleviate endoplasmic reticulum stress by regulating calcium balance, thus inhibiting the apoptosis of chondrocyte.…”
Get full text

Perfluorooctanoic acid induces abnormal heart development via aryl hydrocarbon receptor-mediated oxidative stress and apoptosis in zebrafish larvae by Xueyi CHEN, Tianchi MA, Kang WANG, Tao CHEN, Yan JIANG

“…The activity of AHR was measured using 7-ethoxyresorufin-O-dealkylation (EROD) staining. The levels of intracellular and mitochondrial ROS in the heart of zebrafish larvae were assessed using dichloro-dihydro-fluorescein diacetate and MitoSOX™ Red, respectively. …”
Get full text

Mechanism of Bushen Huoxue Recipe on Improving Arterial Calcification in Rats with Osteoporosis Based on OPG/RANKL Signaling Pathway by LIN Xiaorong, YANG Na, ZHENG Bei, ZHAO Hu, XU Junxia

“…Dual-energy X-ray absorptiometry was used to measure the bone mass density (BMD) of femoral shaft of right hindlimb; alizarin red staining was used to detect integrated optical density (IOD) of calcification deposition on the vascular wall of thoracic aorta, and the calcification degree of thoracic aorta was observed; ELISA method was used to detect serum levels of PⅠNP, BGP and CTX-Ⅰ; immunohistochemical staining was used to detect the expression of OPG and RANKL proteins in thoracic aorta.Results1) BMD and IOD value of thoracic aorta calcification deposition: compared with the control group, there were no significant changes of BMD and IOD value in thoracic aorta calcification deposition of the sham operation group (<italic>P</italic>&gt;0.05), while the BMD of the osteoporosis group decreased significantly, and the IOD value of thoracic aorta calcification deposition increased significantly (<italic>P</italic>&lt;0.05). …”
Get full text

Idiopathic Bilateral Bloody Tearing by Emrullah Beyazyıldız, Yasemin Özdamar, Özlem Beyazyıldız, Hasan Yerli

“…Bloody tearing was not associated with her menses. A blood-stained discharge from the punctum was not observed during the compression of both nasolacrimal ducts. …”
Get full text

The Peripherin Gene Regulates the Migration of Bone Marrow Mesenchymal Stem Cells in Wuzhishan Mini Pigs by Zheng Feng, Wentong Li, Ying Xia, Hui Yu, Hua Li, Kui Li, Yulian Mu

“…The migration capacity of the BMMSCs was estimated using a scratch assay, a transwell in vitro migration model assay, and filamentous actin staining. The results showed that shRNA-mediated knockdown of the expression of the PRPH gene in BMMSCs reduced the ability of these cells to migrate. …”
Get full text

Peroxisome Proliferator-Activated Receptor-𝛾 Is a Potent Target for Prevention and Treatment in Human Prostate and Testicular Cancer by Masahide Matsuyama, Rikio Yoshimura

“…In this study, we examined the expression of PPAR-𝛾 in prostate cancer (PC) and testicular cancer (TC) by RT-PCR and immunohistochemistry, and we also examined the effect of PPAR-𝛾 ligand in these cells by MTT assay, hoechest staining, and flow cytometry. PPAR-𝛾 expression was significantly more extensive and intense in malignant tissues than in normal tissues. …”
Get full text

The Utility of Endoscopic Ultrasound Guided Fine Needle Aspiration in the Diagnosis of Infectious Diseases—Report of Three Cases by Mauro Ajaj Saieg, Felipe Yazawa, Manuela Horta, Lucio G. Rossini, Mabel Tatty Medeiros Fracassi, Fabíola Del Carlo Bernardi

“…In conclusion, EUS was successful not only in obtaining enough cells for morphological analysis, but also for the production of cell blocks and assessment of the presence of the microorganisms by special stains. EUS allied to fine needle biopsy was an important tool in determining diagnoses of enlarged lymph nodes, revealing the diagnosis of infectious diseases in cases otherwise seen as malignant. …”
Get full text

Central nervous system involvement in Whipple's disease: Report of a rare pathological entity and comparative review of treatment strategies and outcomes by Moustafa A. Mansour, MD, MSc, MPhil, Reem W. Malaeb, BS, Islam E. Elnemr, MD, Mohamed Abdel-Fattah El-Salamoni, MD, Hamdi Nabawi Mostafa, MD, Zarina Ahmadi, MD

“…Diagnosis was confirmed through duodenal biopsy with PAS staining and PCR analysis. The patient was successfully treated with a combination of intravenous ceftriaxone followed by oral doxycycline and hydroxychloroquine, resulting in significant symptom improvement and full recovery of neurological function. …”
Get full text

Differential Downregulation of E-Cadherin and Desmoglein by Epidermal Growth Factor by Miquella G. Chavez, Christian A. Buhr, Whitney K. Petrie, Angela Wandinger-Ness, Donna F. Kusewitt, Laurie G. Hudson

“…Dual immunofluorescence staining revealed that neither E-cadherin nor desmoglein-2 internalized with the EGF receptor, or with one another. …”
Get full text

Synergetic Effects of Plasmodium, Hookworm, and Schistosoma mansoni Infections on Hemoglobin Level among Febrile School Age Children in Jawe Worda, Northwest Ethiopia by Tadesse Hailu, Mulat Yimer, Wondemagegn Mulu, Bayeh Abera

“…Plasmodium and helminths infections were detected using Giemsa stain and formol-ether concentration techniques, respectively. …”
Get full text

Reporting a Rare Case of Pleomorphic Adenoma of the Breast by Tahere Khamechian, Javad Alizargar, Tahere Mazoochi

“…After an excisional biopsy, the pathologist found proliferation in epithelial and myoepithelial cells that had small and multiple nuclei, myxoid and chondroid stroma. Immunohistochemistry stain was positive for S-100 and patchy for GFAP in tumor cells and for SMA around the tubule-glandular and tumor cell aggregates and suggested PA of the breast. …”
Get full text

Investigation of relationships between biochemical polymorphism and some production charecters on local breeds and cross breeds of sheep in konya central animal research institute by Mehmet Nizamlıoğlu, Vahdettin Altunok, İnci Togan, Firuze Kurtoğlu, Zafer Bulut, Hüseyin Düzgün

“…For each of the enzyme systems appropriate buffers and chemical for the activity staining were used in accordance with the information given in the literature. …”
Get full text

Tuberculous Panophthalmitis with Lymphadenitis and Central Nervous System Tuberculoma by Sirawat Srichatrapimuk, Duangkamon Wattanatranon, Somnuek Sungkanuparph

“…The pathological examination revealed acute suppurative granulomatous panophthalmitis with retinal detachment. Further staining demonstrated acid-fast bacilli in the tissue. …”
Get full text

Understanding Ambiguity in Knowledge, Value and Institutional Structure: A Case Study of Chinese Open Network Community by Yao Jin

“…Analyzing the structural stain from the dimension of knowledge, value, and institutional structure in the network community, multifarious ambiguity is found in Chinese network governance. …”
Get full text

Relapse of Diffuse Large B‐Cell Lymphoma as Painless Masses in the Abdominal Wall Muscles: A Rare Case Report by Somar Mansour, Seif‐Aldin Abdul Rahman, Majd Mansour, Ali Afif, Raghad Hasan, Nader Abdullah, Zuheir Alshehabi

“…It shows the importance of the diagnostic process that combines imaging with histological examination and immune stains for accurate diagnosis.…”
Get full text

Chloroquine Treatment Increases Detection of 5-Fluorouracil-Induced Apoptosis In Vivo by Mukesh K. Nyati, Felix Y. Feng, Vinayak D. Kanade, Rabindranath Nayak

“…This technique resulted in a significant increase in the accumulation of apoptotic cells induced by 5-fluorouracil, as detected by propidium iodide staining in solid and ascitic forms of Ehrlich ascitic tumors and in bone marrow cells. …”
Get full text

Knockdown of Annexin A1 induces apoptosis, causing G2/M arrest and facilitating phagocytosis activity in human leukemia cell lines by Hasan Masyitah, Kumolosasi Endang, Jantan Ibrahim, Jasamai Malina, Nazarudin Norlaili

“…Phagocytosis activity was evaluated using hematoxylin & eosin staining. The ANXA1 expression was significantly downregulated after the knockdown and apoptosis was induced in tested cells. …”
Get full text