GPR137-RAB8A activation promotes ovarian cancer development via the Hedgehog pathway by Chao Tang, Lin Li, Chongying Zhu, Qiang Xu, Zihao An, Shouying Xu, Chao Lin

“…The biological effects of GPR137-RAB8A-Hedgehog(HH) were investigated using in vitro and in vivo models and methods including qRT-PCR, RNA stability assay, RNA immunoprecipitation assay, GLI-luciferase reporter assay, nucleo-cytoplasmic separation assay, membrane-cytoplasmic separation assay, western blot, co-immunoprecipitation, immunofluorescence staining, cell counting kit-8 assay, wound healing assay, matrigel invasion assay, colony formation assay, xenografts assay, in situ transplantation tumor model of ovarian cancer in nude mice, and immunohistochemistry staining. …”
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Inhibition of the mTOR Pathway Exerts Cardioprotective Effects Partly through Autophagy in CLP Rats by Wen Han, Hao Wang, Longxiang Su, Yun Long, Na Cui, Dawei Liu

“…After treatment, pathological changes were determined by H&E staining, cardiac functions by echocardiography, the distribution of microtubule-associated protein light chain 3 (LC-3) type II and hypoxia-inducible factor 1α (HIF-1a) by immunohistochemical staining, and autophagic vacuoles by transmission electron microscopy. …”
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miRNA-541-5p regulates myocardial ischemia–reperfusion injury by targeting ferroptosis by ZhiYu Zhao, BoXia Li, DianWei Cheng, YuFang Leng

“…ELISA kits quantified MDA, SOD, GSH, LDH, and ferritin levels. TTC staining evaluated infarction size. HE staining observed histopathological changes. …”
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Migrasomes derived from human umbilical cord mesenchymal stem cells: a new therapeutic agent for ovalbumin-induced asthma in mice by Weifeng Gu, Tingting Zheng, Wen Li, Xinkai Luo, Xiaowei Xu, Ying Wang, Chaoming Mao, Yongbin Ma, Liyang Dong

“…The therapeutic effects of migrasomes on airway inflammation in ovalbumin (OVA)-induced asthmatic mice were evaluated by hematoxylin-eosin (HE) and periodic-acid schiff (PAS) staining, and their mechanism were further testified by immunofluorescent staining, real-time PCR and flow cytometry. …”
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Nucleic-Acid-Binding Chromophores as Efficient Indicators of Aptamer-Target Interactions by Kwabena Sarpong, Bhaskar Datta

“…We describe the use of nucleic-acid-staining reagents as an effective tool for detecting and signaling aptamer-target interactions. …”
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Prepatellar Bursal Infection Caused by Mycobacterium tuberculosis with an In Situ Total Knee Arthroplasty: A Case Report and Comprehensive Literature Review by Abdulrahman D. Algarni

“…Aspiration of the bursa revealed acid-fast bacilli on Ziehl–Neelsen staining, typical of Mycobacterium tuberculosis; this was confirmed later on culture. …”
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Alveolar Proteinosis in COVID-19: Clinical Case by Silvia Martin Bote, Maria Angeles Herrera Morueco, Beatriz Arias Arcos, Javier García Lopez, Maria Belen Lopez-Muñiz Ballesteros

“…Bronchoscopy revealed milky bronchoalveolar lavage fluid, and staining with periodic acid–Schiff was positive, thus indicating PAP. …”
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What is the student's level? Analyzing Students' English Proficiency Levels in High Education Level by Burhanudin Rais, Luthfi Awwalia

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Primary Amoebic Meningoencephalitis in an Iranian Infant by Zahra Movahedi, Mohammad Reza Shokrollahi, Mohammad Aghaali, Hosein Heydari

“…Though occurrence of PAM is rare, this unusual disease has grave prognosis, so infection with free living amoebas must be considered in differential diagnosis of pediatric patients of purulent meningitis without evidence of bacteria on Gram’s stain and imaging findings, nonspecific brain edema on CT or hydrocephalus even without history of contact.…”
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Deconstructing the Israeli-Palestinian Conflict in Online News Media: A Critical Discourse Analysis by Alfainy Darojat, Daniel Ari Widhiatama

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A NEW METHOD FOR PREDICTING FATIGUE LIFE UNDER TENSION-TORSIONAL LOADING by ZHANG YanJun, LEI MeiRong, SU Fang

“…The established nonlinear fatigue damage model based on the theory of damage mechanics has been widely applied to multiaxial fatigue life prediction,however,this method does not consider the physical significance in the damage plane.This problem can be solved by combining with the critical plane method.Taking the thin-walled cylindrical specimen as the study object,a new damage parameter applicable to proportional and nonproportional multiaxial fatigue damage is proposed by means of von Mises rule based on the critical plane method with consideration of the influences of phase delay on the fatigue life are analyzed as well.The new defined damage parameter not only considers the maximum shear stain and normal strain on the critical plane,but also considers the effect of the additional cyclic hardening caused by the nonproportional loading on the fatigue life.The fatigue life predicted by the proposed damage model of three types of materials are proved to be in good agreement with experimental results.It is convenient for engineering application because the uniaxial fatigue data and material constants are considered only in this model.…”
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TSALATS MARAHIL LI TATHWIR TA'LIM AL-LUGHAH AL-ARABIYYAH FI INDONESIA by Muhajir Muhajir

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A Systematic Literature Review of Online Peer Feedback: Uncovering the Current Situation in EFL Setting by Sibakhul Milad Malik Hidayatulloh

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Investigation of genetic structure of angora goat by using starch gel electrophoretic method by Vahdettin Altunok, Mehmet Nizamlıoğlu, Zafer Bulut

“…For each of the enzyme systems appropriate buffers end chemicals for the activity staining were used in accordance with the information given in the literature. …”
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A Multimodal Discourse Analysis on Street Graffities about Indonesian Police by Rifpan Putra Afriansyah, Eva Tuckyta Sari Sujatna, Nani Darmayanti

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Multilayer Super-Translucent Zirconia for Chairside Fabrication of a Monolithic Posterior Crown by Sven Rinke, Anna Metzger, Holger Ziebolz

“…The usage of a multilayer super-translucent material allows for the individualization of the restoration, typically in a single combined stain and glaze firing. However, it should be noted that clinical data for this type of restoration are still sparse. …”
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Fatal Balamuthia mandrillaris Encephalitis by Binoy Yohannan, Mark Feldman

“…She underwent right frontal craniotomy with removal of tan, creamy, partially liquefied necrotic material from the brain, consistent with granulomatous amoebic encephalitis on tissue staining. Immunohistochemical studies and PCR tests confirmed infection with Balamuthia mandrillaris. …”
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Deep-Learning-Guided Point Cloud Modeling with Applications in Intelligent Manufacturing by Guifeng Wang, Ning Shuigen, Jianzhang Xiao

“…Protector shape for detailed discoloration data on constructed prominence surfaces for automatic rifle recognition is conducted. Spot staining data sample library is also constructed. The prosecuting feature recognizer gained advantages through sample training, which realized robot-style notification of 36 processing shapes. …”
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Confirmatory identification and antibiotics resistance profiling of Pseudomonas aeruginosa: a laboratory-based study by Hafsa Abdulhamid Abdulsalam

“…Five isolates of Pseudomonas aeruginosa were collected from the Ahmadu Bello University Teaching Hospital, Shika Zaria and subjected to Gram staining and biochemical tests for initial identification. …”
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Macular Amyloidosis and Epstein-Barr Virus by Yalda Nahidi, Naser Tayyebi Meibodi, Zahra Meshkat, Narges Nazeri

“…Amyloidosis is extracellular precipitation of eosinophilic hyaline material of self-origin with special staining features and fibrillar ultrastructure. Macular amyloidosis is limited to the skin, and several factors have been proposed for its pathogenesis. …”
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