Oncolytic influenza virus enhances killing effect of gemcitabine against pancreatic cancer cells by MENG Zihe, MENG Zihe, XU Yongru, DENG Zhaoda

“…Multi-immunofluorescence staining was employed to analyze T cell infiltration and proliferation in the tumor tissues. …”
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Fu Tu Sheng Jin Rehabilitation Formula Mitigate Airway Inflammation, Mucus Secretion and Immune Dysfunction Induced by SARS-CoV-2 Spike Protein by Wang BH, Yu KY, Zhang XN, Sun XH, Tang LL, Shi XL

“…Observe the pathological changes in lung tissues, expression of inflammatory factors, and mucins in different groups of mice using HE and PAS staining methods, as well as ELISA and RT-qPCR. Flow cytometry was used to detect T helper 17 (Th17)/regulatory T (Treg) cells and T helper 1(Th1)/T helper 2 (Th2) lymphocyte ratios and the proportions of conventional myeloid dendritic cells (cDCs), plasma cell-like DCs, CD80 and CD86 cells in mouse spleens.Results: HE and PAS staining showed that, compared to that in the normal group, the lung tissue of the D1 group mice showed a significant inflammatory damage response, whereas the D3 and D10 groups showed a gradual recovery trend. …”
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An Unusual Case of Cystic Fibrosis Associated Pneumocystis jiroveci Pneumonia in an Infant by Ravinder Kaur, Priyanka Katariya, Megh Singh Dhakad, Bhanu Mehra, Urmila Jhamb, A. P. Dubey

“…Subsequently, an immunofluorescence staining of BAL fluid was performed and P. jiroveci cysts were detected. …”
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Exploring the efficacy of AMACR, ERG, and AR immunostains in prostatic adenocarcinoma and their association with novel grade groups by Mohamed O. Andarawi, Hassan Otifi, Hesham Hassan, Adil A. Yousif, Saadalnour A. Mustafa, Shawgi A. Elsiddig, Asad Ma Babker, Elryah I. Ali, Omer Osman Elhag

“…Differential expression was observed among immunomarkers and grade groups of malignancy. AMACR and ERG stains serve as sensitive and specific markers for PCa diagnosis and prognosis. …”
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The Visualization of Biofilms in Chronic Diabetic Foot Wounds Using Routine Diagnostic Microscopy Methods by Angela Oates, Frank L. Bowling, Andrew J. M. Boulton, Philip G. Bowler, Daniel G. Metcalf, Andrew J. McBain

“…Results in this pilot study indicate that bacterial microcolonies and putative biofilm matrix can be visualized in chronic wounds using florescence microscopy and ESEM, but also using the simple Gram stain.…”
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Multiorganelle Localization of Metallated Phthalocyanine Photosensitizer in Colorectal Cancer Cells (DLD-1 and CaCo-2) Enhances Efficacy of Photodynamic Therapy by Palesa Rose Sekhejane, Nicolette Nadene Houreld, Heidi Abrahamse

“…ZnPcSmix was predominantly localized in lysosomes and partially in the mitochondria. FITC Annexin V staining showed a significant decrease in the percentage of viable DLD-1 and CaCo-2 cells 24 h after PDT, with an increase in apoptotic cell population. …”
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Lone Hepatocellular Carcinoma: An Isolated Chest Wall Malignancy by Joseph Allencherril, Sebastian Bruera, Ronan Allencherril, Richard J. Hamill

“…Pathological examination and immunohistochemical staining of the chest mass biopsy were consistent with metastatic hepatocellular carcinoma (HCC). …”
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Upper Gastrointestinal Bleeding from Gastric Amyloidosis in a Patient with Smoldering Multiple Myeloma by Mihajlo Gjeorgjievski, Treta Purohit, Mitual B. Amin, Paul J. Kurtin, Mitchell S. Cappell

“…Based on this and multiple other case reports, we recommend that patients with GI bleeding and MGUS, SMM, or MM undergo EGD and pathologic examination of endoscopic biopsies of identified lesions using Congo red stains for amyloidosis for early diagnosis and treatment.…”
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Antihepatitis B Virus Activity of a Protein-Enriched Fraction from Housefly (Musca domestica) in a Stable HBV-Producing Cell Line by Xuemei Lu, Xiaobao Jin, Jie Wang, Fujiang Chu, Jiayong Zhu

“…HBV core protein was assayed by immunofluorescent staining. Results indicate PE treatment inhibited both HBsAg, HBeAg secretion, and HBV-DNA replication. …”
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Quantification and identification of bacterial presence in salted eggs by Zukhrufa Vista Vindriati, Usamah Afiff, Trioso Purnawarman

“…The total bacterial count was determined using the plate count agar method, and bacterial identification was based on phenotypic analysis, which included Gram staining and biochemical tests. Results The total bacterial load in fresh salted eggs was below the maximum limit set by SNI 7388:2009 (1×105 CFU/g), whereas the pasta dough and black egg yolk exceeded this limit. …”
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Meningococcal Neonatal Purulent Conjunctivitis/Sepsis and Asymptomatic Carriage of N. meningitidis in Mother’s Vagina and Both Parents’ Nasopharynx by Enrique Chacon-Cruz, Jorge Arturo Alvelais-Palacios, Jaime Alfonso Rodriguez-Valencia, Erika Zoe Lopatynsky-Reyes, Maria Luisa Volker-Soberanes, Rosa Maria Rivas-Landeros

“…Clinical examination confirmed profuse purulent conjunctival discharge, as well as clinical signs and laboratory findings suggestive of bacteraemia. Gram stain from conjunctival exudate revealed intracellular Gram negative diplococci; we presumed the baby had gonorrheal conjunctivitis; however, serogroup Y, N. meningitidis was isolated both from conjunctival exudate and blood. …”
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A Technique for Section Thickness Evaluation for Microphotometry and Image Analysis of Sectioned Nuclei by R. L. Cabrini, A. Folco, S. Orrea, M. T. Savino, A. M. Schwint, M. E. Itoiz

“…The standards consist of celloidin cylinders stained with the PAS reaction and embedded in paraffin. …”
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Diagnosing Cardiac Amyloidosis: From Heart Failure to Electrical Storm by Adithya T. Mathews, Abu-Sayeef Mirza, Chandrashekar Bohra, Akshay G. Mathews, Philip Ritucci-Chinni, Jamie L. Weber, William W. Bulkeley, Maqsood Siddique, David Whitaker, Richard Bowerman

“…Autopsy revealed gross cardiomegaly and left ventricular hypertrophy with Congo red staining revealing amyloid fibrils with apple-green birefringence. …”
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Expression of Semaphorin 3F and Its Receptors in Epithelial Ovarian Cancer, Fallopian Tubes, and Secondary Müllerian Tissues by Christina D. Drenberg, Sandra Livingston, Ren Chen, Patricia A. Kruk, Santo V. Nicosia

“…Tissue sections were evaluated for staining intensity and percentage of immunoreactive epithelia. …”
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Purification and Characterization of a Novel Intracellular Sucrase Enzyme of Leishmania donovani Promastigotes by Arpita Singh, Debjani Mandal

“…The estimated size of intracellular sucrase is ~112 kDa by gel filtration chromatography, native PAGE, and substrate staining. However, in SDS-PAGE, the protein is resolved at ~56 kDa, indicating the possibility of a homodimer in its native state. …”
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Iodine test to detect physical damage in chickpea seeds by Jose de Oliveira Cruz, Luís Carlos de Carvalho Júnior, Ricardo Carmona, Jônatas Barros dos Santos, Isabella Fuckner Artiaga

“…With the aim of developing a protocol for rapid iodine testing, the aim of this study was to determine the most suitable exposure time for chickpea seeds in iodine solution, and the most appropriate concentration for staining naturally or artificially damaged seeds. Concentrations of 5%, 10% and 15% (commercial product containing 2% iodine) were tested on both intact and artificially damaged seeds for an immersion time of 2.5, 5.0, 10.0, 15.0 and 20.0 minutes. …”
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Resveratrol-loaded PCL-PEG/GO/HAP biocomposite bone membranes: Evaluation of mechanical properties, release kinetics, and cellular response by Betül Meryem Arpacay, Fatih Ciftci, Ali Can Özarslan, Mustafa Unal, Mine Kucak, Aslihan Yelkenci

“…In vitro cell adhesion studies, visualized using DAPI fluorescence staining, demonstrated increased cell adhesion to the composite membranes over periods of 1, 3, 5, 7, and 14 days. …”
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Solitary Fibrous Tumor of Neck Mimicking Cold Thyroid Nodule in 99m Tc Thyroid Scintigraphy by Oya Topaloglu, Bekir Ucan, Taner Demirci, Muyesser Sayki Arslan, Guleser Saylam, Evrim Onder, Sinan Gultekin, Alper Dilli, Mustafa Sahin, Erman Cakal, Mustafa Ozbek, Tuncay Delibasi

“…Immunohistochemistry showed positive reaction for CD34 in spindle cells and diffuse bcl-2 staining. The pathology was confirmed as solitary fibrous tumor. …”
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The Dynamic DNA Demethylation during Postnatal Neuronal Development and Neural Stem Cell Differentiation by Huikang Tao, Pei Xie, Yuhang Cao, Liqi Shu, Liping Li, Junchen Chen, Guangfeng Tian, Yingliang Zhuang, Qiang Shu, Xuekun Li

“…In this study, utilizing DNA dot blot, immunofluorescence staining, and qRT-PCR, we studied the expression pattern of Tets, the enzymes governing DNA demethylation, and the levels of 5hmC, 5fC, and 5caC during the postnatal neuronal development of mice. …”
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Detection of Mechanically Recovered Poultry Meat (MRPM) in Traditional Egyptian Luncheon (Emulsion Type Sausage) by Mohamed Mai A., Zahran Dalia A., Kassem Gehan M.A., Emara M.M.T., Mansour N.M.

“…Commercial and experimentally produced emulsion type sausage (traditional Egyptian luncheon) formulated with 0, 10, 30, 50, 70, 90% MRPM instead of the meat mass and cooked to different core temperature (70, 80 and 90°C) were examined for technological properties, ash, bones, cartilage and calcium (Ca) content, in addition to histological sections stained with H&E and Trichrome blue. Results indicated that all market samples showed unacceptable texture and binding scores with high ash, collagen, cartilage, bone and Ca content. …”
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