Intraperitoneal Granulomas Unexpectedly Found during a Cesarean Delivery: A Late Complication of Dropped Gallstones by David A. Suarez-Zamora, Luis E. Barrera-Herrera, Ricardo Caceres-Mileo, Mauricio A. Palau-Lazaro

“…Histological examination demonstrated a bile-stained material and a foreign body-type granulomatous response without neoplastic tissue. …”
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Enamel Based Composite Layers Deposited on Titanium Substrate with Antifungal Activity by S. L. Iconaru, A. M. Prodan, C. S. Turculet, M. Beuran, R. V. Ghita, A. Costescu, A. Groza, M. C. Chifiriuc, P. Chapon, S. Gaiaschi, R. Hristu, G. A. Stanciu, R. Trusca, M. Ganciu, S. M. Raita, N. Vineticu, C. S. Ciobanu

“…The Candida albicans biofilm development on the obtained layers was examined after 24 h, 48 h, and 72 h by confocal laser scanning microscopy (CLSM) and scanning electron microscopy (SEM) after ethidium bromide staining. A significant inhibition of the fungal adherence and biofilm development was observed on Ti-Enamel layers. …”
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A Definitive Diagnosis of Primary Hodgkin Lymphoma on Endoscopic Biopsy Material Utilizing in-Depth Immunohistochemical Analysis by Garnet Horne, Shaun AC Medlicott, Adnan Mansoor, Johan Lategan, Raymond Lai, P Beck

“…A case of a gastroesophageal junction primary HL is presented that was diagnosed on endoscopic biopsy material with the assistance of the immunohistochemical stains PAX5 and MUM1 which verified the presence of Reed-Sternberg cells. …”
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A Neonate with CLOVES Syndrome by Dilek Sarici, Mustafa Ali Akin, Selim Kurtoglu, Filiz Tubas, Serdar Umit Sarici

“…We herein report a very rare case of CLOVES syndrome with the findings of lipomatous overgrowth in the cheek (facial asymmetry), vascular malformation (hemangiomas), epidermal nevi (large port wine stains), and skeletal abnormalities (widened first interdigital space, dystrophia in the nail of the first digit of the right foot, and bilateral hypertrophy of the first digits of the feet).…”
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Protocol for inducing beige adipocytes in white adipose tissue of mouse using cold exposure and CL316,243 injection by Yalan Wu, Chenguang Wang, Xiao Yu Tian

“…We then detail procedures for histology, whole-mount immunofluorescence (IF) staining, and extracting RNA and protein. This protocol can be used for subsequent analysis to explore the mechanisms governing beige WAT induction in experimental settings, particularly the evaluation of angiogenesis.For complete details on the use and execution of this protocol, please refer to Wang et al.1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.…”
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Application of Autofluorescence Endoscopy for Colorectal Cancer Screening: Rationale and an Update by Hiroyuki Aihara, Hisao Tajiri, Takeshi Suzuki

“…Autofluorescence endoscopy (AFE) can detect slight alterations in their distribution and concentration during the colorectal carcinogenesis process and, thus facilitate noninvasive screening colonoscopies without the need for fluorescent substances or staining reagents to be administered. While detecting faint autofluorescence intensity by conventional fiberoptic endoscopy remains challenging, the latest AFE system with high-resolution videoendoscope capabilities enables such detection by using a false-color display algorithm. …”
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Invasive pathogens impacting forest and urban trees in Türkiye by Tuğba Doğmuş Lehtijarvi, Stephen Woodward

“…Examples of invasive pathogens, including the causes of sweet chestnut canker, Dutch elm disease, dieback of Cupressus, Phytophthora root rots, boxwood blight, canker stain (wilt) of Platanus and Dothistroma needle blight of pines are detailed. …”
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Integration and acceptability of cervical cancer screening as part of postnatal care among women attending postnatal clinics in the Accra Metropolis, Greater Accra Region, Ghana. by Lawrence Oppong Buadi, Raphael Odame, Ali Samba, Mumuni Kareem, Angela Amoanimaa Boateng, Claudette Ahliba Diogo, Rebecca Tricia Morrision, George Kumi Kwakye, Cecilia Smith-Togobo

“…The samples were sent to the cytology laboratory and stained with the Pap staining technique. All the smears were examined and reported by the cytology department of Korle-Bu Teaching Hospital. …”
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Performance of HPV E4 and p16INK4a biomarkers in predicting regression of cervical intraepithelial neoplasia grade 2 (CIN2): protocol for a historical cohort study by David Jenkins, Mark H Stoler, Anne Hammer, Torben Steiniche, Lone Kjeld Petersen, Patti E Gravitt, Rikke Kamp Damgaard, Maurits NC de Koning, Wim GV Quint, John Doorbar, Johnny Kahlert

“…Tissue samples from women included will be sectioned for p16INK4a and HPV E4 immunohistochemical staining in addition to conventional hematoxylin and eosin (H&E) staining. …”
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hCeO2@CA-074Me Nanoparticles Alleviate Inflammation and Improve Osteogenic Microenvironment by Regulating the CTSB-NLRP3 Signaling Pathway by Niu Z, Xia X, Zhang Z, Liu J, Li X

“…The osteogenic ability was evaluated using ALP staining, ALP quantitative analysis, alizarin red staining, and RT-PCR analysis.Results: Findings clearly demonstrated that hCeO2@CA-074Me NPs could effectively reduce the production of ROS and inhibited CTSB-NLRP3 signal pathway, thereby significantly attenuating the damage caused by the cellular inflammatory response. hCeO2@CA-074Me NPs could also induce the polarization of macrophages towards anti-inflammatory M2 phenotype. …”
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DYNLT3 overexpression induces apoptosis and inhibits cell growth and migration via inhibition of the Wnt pathway and EMT in cervical cancer by Jianan Zhang, Qi Shen, Lu Xia, Xueqiong Zhu, Xuejie Zhu

“…Immunohistochemical staining was used to detect DYNLT3 protein expression in cervical cancer tissues. …”
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AM1241 inhibits chondrocyte inflammation and ECM degradation through the Nrf2/HO-1 and NF-κB pathways and alleviates osteoarthritis in mice by Zhuan Zou, Songmu Pan, Changzheng Sun, Jiyong Wei, Yi Xu, Kaizhen Xiao, Jinmin Zhao, Ronghe Gu

“…In vivo, the impact of AM1241 on OA mice was analyzed through safranin-fast green staining, IHC staining, Mankin score, and microCT. …”
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Scutellarein Inhibits Osteosarcoma Growth by Targeting the TLR4/TRAF6/NF-κB Pathway by Shi Y, Tang Y, Sun Z, Sui P, Shao Y, Wang Z, Zhang J, Gao M

“…The effects of SCU and TLR4 overexpression on OS growth were analyzed using a xenograft tumor model and immunohistochemical staining.Results: SCU was found to significantly inhibit OS cell proliferation, and RNA sequencing analysis suggested that the NF-κB pathway is closely associated with this process. …”
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Effect and Mechanism of Catalpol on Behavior in Rats with Post-stroke Depression by WANG Xiangyu, ZHOU Xia, LIU Ju, LIU Fuhong, ZHANG Jiale, SUN Liping, LI Wanbin

“…The behavior of the rats was evaluated by elevated plus maze measurement. TTC staining was used to detect cerebral infarction volume. …”
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Evaluation of the protective role of resveratrol on LPS-induced septic intestinal barrier function via TLR4/MyD88/NF-κB signaling pathways by Zhongliang Shi, Yanna Jiao, Zhizhen Lai, Juan Liu, Bo Yang, Mahong Hu, Jianbiao Meng

“…Various techniques including enzyme-linked immunosorbent assay (ELISA), hematoxylin and eosin staining (HE), periodic acid Schiff (PAS) staining, transmission electron microscopy (TEM), Western blot analysis (WB), and quantitative real-time polymerase chain reaction (qRT-PCR) were utilized to assess differences in inflammatory cytokine expression, histopathological changes, apoptosis, tight junction (TJ) protein, and the TLR4/MyD88/NF-кB signaling pathways. …”
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Verbascoside Inhibits the Activation of NLRP3 Inflammasome and Improves BV-2 Cell Inflammatory Damage by Wenjing HUANG, Fang CHENG, Min DU, Jiayi CHEN, Wei XIANG, Ke SHI, Xingliang XIE, Yanmei SHENG

“…Hoechst 33342/PI double staining was utilized to examine the effect of the treatments on cell pyroptosis. …”
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Effect of Qingjie Fuzheng Granules on Proliferation, Apoptosis and Migration of Colorectal Cancer SW620 Cells by Xiaoqin ZHU, Hong YANG, Bin HUANG, Lihui WEI, Jiumao LIN

“…Apoptosis of SW620 cells after 24 h was detected by Hoechst 33258 staining test. The migration ability of cells after 0 h,6 h,12 h and 24 h was detected by scratching test. …”
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Zoning of the reaction of the tissues of the implantation area after prosthetic repair in abdominal hernias in experiment by E. E. Lukoyanychev, S. G. Izmailov, D. A. Evsyukov, V. O. Nikolskij, A. A. Mironov, A. V. Panyushkin

“…The preparation and staining of each preparation was carried out in two versions: with hematoxylin-eosin and according to Mallory on different glasses. …”
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Staphylococcus aureus vesicles impair cutaneous wound healing through p38 MAPK-MerTK cleavage-mediated inhibition of macrophage efferocytosis by Jiaxin Ou, Kangxin Li, Hui Yuan, Shaohua Du, Tingting Wang, Qiannan Deng, Huimei Wu, Weiyan Zeng, Kui Cheng, Kutty Selva Nandakumar

“…Macrophage efferocytosis efficiency was assessed using flow cytometry and confocal microscopy, while efferocytosis at wound sites was analyzed through WB, FACS, and TUNEL staining. Hematoxylin and eosin (H&E) staining and wound size measurements were used to evaluate the wound healing process. …”
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Inhibition of id-1 reduces osteosarcoma growth and metastasis through mediation of snail by Rongbing Shu, Zhuanyi Yu, Jianmin Wu, Qiuxin Cheng, Zhihao Peng, Huaqiang Zhou, Min Zhao

“…Tumor growth and lung metastasis were observed using HE staining. The expression of Id-1, Snail, and EMT-related proteins in osteosarcoma and lung tissues from each group of mice was assessed using Western blot and immunohistochemistry. …”
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