Effect of supplementing epinephrine in maturation media on the developmental competence of buffalo oocytes by Ali Husnain, Abdul Khaliq, Talha Ashraf, Abdul Rehman, Muhammad Yasir Zahoor, Muhammad Binyameen, Amjad Riaz

“…The maturation of oocytes was examined based on the nuclear maturation after staining with Hoechst 33342 (Sigma Aldrich, St. Louis, MO, USA). …”
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Electroacupuncture Regulates Disorders of Gut-Brain Interaction by Decreasing Corticotropin-Releasing Factor in a Rat Model of IBS by Ying Chen, Yan Zhao, Dan-ni Luo, Hui Zheng, Ying Li, Si-yuan Zhou

“…Compared with the blank control group, visceral pain threshold pressure, the expression of ZO-1, OT%, SP%, CRF, and CRF-R1 mRNA expression in the hypothalamus, and double staining of IMMC and CRF-R1 were decreased significantly in the CUMS group. …”
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CEST imaging combined with 1H-MRS reveal the neuroprotective effects of riluzole by improving neurotransmitter imbalances in Alzheimer’s disease mice by Yuanyu Shen, Xiaolei Zhang, Siqi Liu, Lijing Xin, Wentao Xuan, Caiyu Zhuang, Yue Chen, Beibei Chen, Xinhui Zheng, Renhua Wu, Yan Lin

“…At the study endpoint, immunohistochemistry, Nissl staining, and Western blot were used to evaluate the brain pathology, neuronal survival, and protein expression. …”
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Microplastic analysis in soils: A comparative assessment by Stoyana Peneva, Quynh Nhu Phan Le, Davi R. Munhoz, Olivia Wrigley, Flora Wille, Heidi Doose, Crispin Halsall, Paula Harkes, Michael Sander, Melanie Braun, Wulf Amelung

“…Yet, our data indicate that adding stained large MiP as an internal standard could enhance extraction control, while Soxhlet-extraction with subsequent 1H NMR analysis is most powerful for controlling future thresholds of small MiP from biodegradable materials.…”
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Proteomic Analysis of Hypoxia-Induced Senescence of Human Bone Marrow Mesenchymal Stem Cells by Liping Mai, Guodong He, Jing Chen, Jiening Zhu, Shaoxian Chen, Xinghua Hou, Hui Yang, Mengzhen Zhang, Yueheng Wu, Qiuxiong Lin, Min Yang, Xiaohong Li

“…Cell apoptosis was evaluated using Annexin V/7-AAD staining by flow cytometry. The production of reactive oxygen species (ROS) was detected by the fluorescent probe 2,7-dichlorodihydrofluorescein diacetate (DCFH-DA). …”
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MLL4 regulates postnatal palate growth and midpalatal suture development by Jung-Mi Lee, Hunmin Jung, Bruno de Paula Machado Pasqua, Yungki Park, Qinghuang Tang, Shin Jeon, Shin Jeon, Soo-Kyung Lee, Jae W. Lee, Hyuk-Jae Edward Kwon

“…Whole-mount and histological staining at perinatal stages identified that the midline defects in the Mll4-cKO mice emerged as early as 1 day prior to birth, presenting as a widened midpalatal suture, accompanied by increased cell apoptosis in the suture mesenchyme. …”
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Sonic hedgehog restrains the ubiquitin-dependent degradation of SP1 to inhibit neuronal/glial senescence associated phenotypes in chemotherapy-induced peripheral neuropathy via the... by Ying Zou, Shu Wu, Qian Hu, Haoxian Zhou, Yuanlong Ge, Zhenyu Ju, Shengkang Luo

“…To understand the underlying mechanisms, we conducted ubiquitin assays, LC-MS/MS, H&E staining, and assessed protein expression through Western blotting and immunofluorescence staining. …”
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The antiarthritic effect of CBR‐470‐1 in hypoxic environment is to increase the level of NOD‐like receptor family pyrin domain containing 3 ubiquitination by decreasing phosphoglyc... by Ao Duan, Zemeng Ma, Xiaolong Shao, Zhencheng Xiong, Chaoyi Zhang, Wenzheng Liu, Guanglin Wang, Shouye Hu, Wei Lin

“…Methods The effects of CBR‐470‐1 were assessed in a mouse model of OA induced by destabilization of the medial meniscus (DMM) by micro‐computed tomography imaging, Safranin‐O and Fast Green staining, immunofluorescence staining and enzyme‐linked immunosorbent assay. …”
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Development of Higher Order Thinking Skills (HOTS) Based Inferential Reading Module by Uyun Nafiah, Wahyuni Fitria, Ayuliamita Abadi

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Metastatic Breast Carcinoma to the Prostate Gland by Meghan E. Kapp, Giovanna A. Giannico, Mohamed Mokhtar Desouki

“…The patient’s previous breast needle core biopsy showed strong ER positivity and negative staining for PR and HER2. Similar to the prostate, the breast was negative for CK5/6, p63, and p40. …”
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Upper Airways Spray for Viral Infections Prevention by Beniamino Palmieri, Maria Vadalà

“…The mucous smear was stained with May Grunwald-Giemsa to exclude eosinophilic infiltrate and confirm the prevalence of granulocytes and lympho-monocytes typical of viral seasonal inflammatory upper airways conditions. …”
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Cutaneous Squamous Cell Carcinoma with Signet-Ring Cell Component and CDX2 Expression in a Patient Treated with PD-1 Inhibitor: A Case Report of a Common Tumor with Unusual Differe... by Sherehan Zada, Jeremiah Tao, Maria Del Valle Estopinal

“…Immunohistochemical studies demonstrated that the tumor cells were positive for P63, CK5/6, CDX2, and P53 while negative for P16, CK7, CK20, and CD68 stains. An abnormal expression of B-catenin was also observed in the tumor. …”
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Tuberculous Panophthalmitis with Lymphadenitis and Central Nervous System Tuberculoma by Sirawat Srichatrapimuk, Duangkamon Wattanatranon, Somnuek Sungkanuparph

“…The pathological examination revealed acute suppurative granulomatous panophthalmitis with retinal detachment. Further staining demonstrated acid-fast bacilli in the tissue. …”
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Understanding Ambiguity in Knowledge, Value and Institutional Structure: A Case Study of Chinese Open Network Community by Yao Jin

“…Analyzing the structural stain from the dimension of knowledge, value, and institutional structure in the network community, multifarious ambiguity is found in Chinese network governance. …”
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Massive Pleural Effusion in an 18-Year-Old Girl with Ewing Sarcoma by Cengiz Özge, Mukadder Çalikoglu, Leyla Cinel, F Demir Apaydin, Eylem S Özgür

“…All of the tumour cell cytoplasms stained with CD99. The pathological examination supported Ewing sarcoma. …”
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An Analysis of Students’ Challenges in Speaking English in the Tenth Grade of Madrasah Aliyah Al-Hariri Tebo by Lili Rahma Yulita, Alfian, Nisaul Fadillah

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Inflammatory Mechanisms of Idiopathic Epiretinal Membrane Formation by Malav Joshi, Shivi Agrawal, John Byron Christoforidis

“…Thus, immunohistochemical techniques that stain for the cytostructural components of cells have confirmed the importance of glial cells and hyalocytes in iERM formation. …”
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Implications Of Ingesting Soft Drink And Energy Drink On Hepatic Microcirculation Of Albino Rat by Saira Munawar, Faiza Irshad, G.P. William, Muhammad Suhail, Ayesha Yousaf, Ahmad Haris Suhail

“…Later, the rats were sacrificed, the liver dissected out, slides prepared and stained with H & E for microscopic observation. …”
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Investigating Students’ Academic Self-Efficacy in Play Performance Class by Thomas Wahyu Prabowo Mukti, Veronica Triprihatmini

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Listeria monocytogenes Meningoencephalitis and Cerebral Abscess in a Heart Transplant Recipient by Paul C. Adjei

“…Lumbar puncture revealed elevated opening pressure, CSF neutrophilic pleocytosis, and elevated CSF protein and lactate but negative gram stain and cultures. First-line agent for Listeria meningoencephalitis is ampicillin. …”
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