Unveiling the potential of spirulina algal extract as promising antibacterial and antibiofilm agent against carbapenem-resistant Klebsiella pneumoniae: in vitro and in vivo study by Mohamed I. Selim, Tarek El-banna, Fatma Sonbol, Walaa A. Negm, Engy Elekhnawy

“…This was manifested by improving the pulmonary architecture, decreasing the inflammatory cell infiltration, and fibrosis after staining with hematoxylin and eosin and Masson’s trichrome stain. …”
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Silencing FOXA1 suppresses inflammation caused by LPS and promotes osteogenic differentiation of periodontal ligament stem cells through the TLR4/MyD88/NF-κB pathway by Miao He, Yangdong Lin

“…Alkaline phosphatase (ALP) staining, alizarin red S staining, and Western blot were employed to evaluate the impact of FOXA1 silencing on the osteogenic differentiation of hPDLSCs. …”
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Electroacupuncture Therapy Effectively Protects the Rat Brain after Intracerebral Hemorrhage by Li Huang, Xuehui Fan, Yao Chen, Heng Lin, Xiaoqian Jiang, Chaoxian Yang

“…Measurement of the brain water content and TUNEL staining were used to evaluate brain edema and cell apoptosis, respectively. …”
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Ascitic Fluid in Ovarian Mass with Special Reference to Paired-box Gene 8 Immunoexpression: A Cross-sectional Analysis by Prabhat Mahato, Chhanda Das, Rama Saha, Ankita Pranab Mandal, Gourisankar Kamilya

“…The formalin-fixed, paraffin-embedded cellblocks were sliced into 4–5 μ sections and stained with hematoxylin and eosin. Subsequently, we used an immunohistochemical stain for PAX8 expression to diagnose ovarian malignancy. …”
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Connexin 37 Regulates the Kv1.3 Pathway and Promotes the Development of Atherosclerosis by Minqi Liao, Lihua Chen, Jiongbin Lu, Guangzhu Liang, Yongzhao Yao, Shumin Ouyang, Yanhua Yang, Zhengwei Jian, Suxia Guo

“…The protein and mRNA expression levels of Cx37 and Kv1.3 were detected by reverse transcription-PCR (RT-PCR), Western blot, and immunofluorescence technique. Oil red O staining was used to observe plaque area. Masson staining was used to detect collagen content. …”
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Diagnostic utility of LEF1 and β-catenin in WNT pathway tumors with CTNNB1 mutation by Can Li, Lingdan Dong, Li Zhu, Wenbin Guan

“…Immunohistochemical staining was identified positive (nuclear staining of LEF1 and β-catenin in > 50% of tumor cells). …”
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Evaluation of the quality characteristics of fermented sausages and sausage-like products sold in kars by Çiğdem Sezer, Aksem Aksoy, Özgür Çelebi, Turgay Deprem, Metin Öğün, Nebahat Bilge Oral, Leyla Vatansever, Abamüslüm Güven

“…Histological analysis was performed by Crossman triple stain and hematoxylin-eosin stains. Physicochemical analysis was performed by gravimetric and spectrophotometric methods.…”
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Selective epithelial expression of KRASG12D in the Oncopig pancreas drives ductal proliferation and desmoplasia that is accompanied by an immune response by Carlos P. Jara, Al-Murtadha Al-Gahmi, Audrey Lazenby, Michael A. Hollingsworth, Mark A. Carlson

“…Desmoplastic responses were evident through vimentin, α-SMA, and Masson’s trichrome staining, indicating progressive collagen deposition, particularly at the higher vector doses. …”
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NETosis Secondary to the Use of Levamisole-Adulterated Cocaine: A Likely Underlying Mechanism of Vasculopathy by Manuela Osorio, Isabel Velásquez, Ruben Vargas, Adriana Vanegas-García, Mauricio Rojas, Gloria Vásquez, Carlos Muñoz-Vahos

“…Cell viability and purity were evaluated by flow cytometry after staining with PI/DiOC6 and labeling with fluorescent anti-CD45/anti-CD3 monoclonal antibodies (mAbs), respectively. …”
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Immunosuppressive Phenotype of Esophagus Tumors Stroma by Olga V. Kovaleva, Madina A. Rashidova, Daria V. Samoilova, Polina A. Podlesnaya, Valeria V. Mochalnikova, Alexei Gratchev

“…For T cell detection, CD8, CD3, and FOXP3 were used. Also, we performed staining for PD-L1 that can be expressed on TAMs and tumor cells. …”
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Cytogenetic factors decreasing the fertility of pollen and cobs during clogging of tetraploid maize with triploid grains (<i>Zea mays</i> L.) by E. B. Khatefov, A. A. Grushin, V. N. Boyko

“…Metaphase plates of maize roots were stained with Schiff’s reagent according to Feulgen and pollen grains were stained with Lugol’s solution.Results. …”
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SDF-1α/OPF/BP Composites Enhance the Migrating and Osteogenic Abilities of Mesenchymal Stem Cells by Linli Li, Xifeng Liu, Bipin Gaihre, Sungjo Park, Yong Li, Andre Terzic, Lichun Lu

“…Scratching assay, Transwell assay, and three-dimensional migration model were utilized to assess the migration response of MSCs. Immunofluorescence staining of Runx2 and osteopontin (OPN), ELISA assay of osteocalcin (OCN) and alkaline phosphatase (ALP), and Alizarin Red S staining were conducted to assess the osteogenesis of MSCs. …”
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Type 2 Diabetic Mellitus Inhibits Skin Renewal through Inhibiting WNT-Dependent Lgr5+ Hair Follicle Stem Cell Activation in C57BL/6 Mice by Minghui Wang, Shangsheng Yao, Dehua He, Mulan Qahar, Jinqing He, Meifang Yin, Jun Wu, Guang Yang

“…Hair follicle regeneration was observed by naked eyes and HE staining. The proliferation of the skin cells was observed by PCNA and K14 staining. …”
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L-Borneol 7-O-[β-D-Apiofuranosyl-(1→6)]-β-D-Glucopyranoside Alleviates Myocardial Ischemia-Reperfusion Injury in Rats and Hypoxic/Reoxygenated Injured Myocardial Cells via Regulati... by Ziyi Tong, Gaowen Li, Chengxiao Su, Liyan Zhou, Ling Zhang, Qun Chen, Qing Xia

“…Myocardial function was detected by an electrocardiograph, histological, TTC, and TUNEL staining analyses. The influences of LBAG on the content concentration of cardiac enzymes in the serum were measured by ELISA. …”
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Ameliorative Impact of Liraglutide on Chronic Intermittent Hypoxia-Induced Atrial Remodeling by Jun Wang, Yongzheng Liu, Changhui Ma, Yue Zhang, Meng Yuan, Guangping Li

“…Masson and Sirius red staining assays were utilized to assess the extent of fibrosis in the atrial tissue of the mice. …”
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Optimization of a Rabbit Dry Eye Model Induced by Topical Instillation of Benzalkonium Chloride by Carlos Carpena-Torres, Jesús Pintor, María Jesús Pérez de Lara, Fernando Huete-Toral, Almudena Crooke, Cristina Pastrana, Gonzalo Carracedo

“…Tear secretion with and without anesthesia, tear breakup time, tear osmolarity, corneal staining, conjunctival hyperemia, density of goblet cells, height of mucin cloud, and transcript levels of IL-6 were measured before and after the treatment. …”
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The role and mechanism of HMGB1-mediated Notch1/Hes-1 pathway in anxiety and depression-like behaviors in mice with chronic rhinosinusitis by Fangwei Zhou, Yiting Jiang, Yangsong Li, Jianyao Li, Tian Zhang, Guodong Yu

“…Techniques including 18F-FDG PET, Nissl staining, and immunofluorescence were used to assess hippocampal metabolic activity in CRS mice. …”
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Ferrostatin-1 inhibits osteoclast differentiation and prevents osteoporosis by suppressing lipid peroxidation by Wenbo Xu, Shiyan Lv, Xiaoyan Wang, Chengchao Song, Chunyang Xi, Jinglong Yan

“…Methods Bone marrow macrophages (BMMs) were extracted from C57BL/6J mice and induced to differentiate into osteoclasts, which were observed via TRAP staining, Phalloidin staining and bone pit assays. …”
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HISTOLOGICAL ANALYSIS OF LYMPHOID MASSES OF THE STOMACH AND SMALL INTESTINE IN THE CLINICAL AND PATHOGENETIC PROFILE OF BABESIOSIS by I. I. Torianyk

“…Haematoxylin and eosin staining gave a clear histological pattern with a marked contrast of fragments, which contributed to diagnostic resources. …”
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Effects of Ginsenoside Rg1 Regulating Wnt/β-Catenin Signaling on Neural Stem Cells to Delay Brain Senescence by Yue Xiang, Shun-he Wang, Lu Wang, Zi-ling Wang, Hui Yao, Lin-bo Chen, Ya-ping Wang

“…The following indicators were detected: (1) NSC culture identification, (2) the effect of LiCl on the proliferation and survival rate of NSCs, (3) the effect of ginsenoside Rg1 on the proliferation and survival of NSCs, (4) the growth of NSCs in each group observed by an optical microscope, (5) the cell cycle of each group detected by flow cytometry, (6) the proliferative ability of each group detected by BrdU, (7) the fluorescence intensity of Nestin and Sox2 of NSCs in each group observed by a fluorescence microscope, (8) the positive rate of senescence staining analyzed by SA-β-Gal staining, (9) the localization of β-catenin in NSCs observed by laser confocal microscopy, and (10) the changes of the Wnt/β-catenin pathway-related proteins in each group detected by Western blotting. …”
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