The Neuroprotective Effects of Astragaloside IV against H2O2-Induced Damage in SH-SY5Y Cells are Associated with Synaptic Plasticity by Zurong Song, Ali Tao

“…The expression of postsynaptic density 95 (PSD-95) and growth-associated protein 43 (GAP-43) were measured by western blot (WB) and inmunofluorescence staining assay under the same treatment conditions. …”
Get full text

Effect of Electroacupuncture at Shenting and Baihui Acupoints on Ability of Learning and Memory and Expression of Autophagy Related Genes and Proteins in Cerebral Ischemia-reperfus... by Lingli GAO, Xiaodong FENG

“…Morris water maze experiment was used to observe learning and memory ability of rats, TTC staining was used to observe the change of brain infarction area of rats, RT-PCR and Western Blot were used to detect the expression of autophagy related genes and proteins in hippocampus.Results:④Morris water maze outcomes showed that compared with the model group, the escape latency time of rats in the EA group was obviously shortened, the number of across-platform was increased and the distance was shortened (<italic>P</italic>&lt;0.05).④Compared with the model group, the nerve defect scale of brain ischemia-reperfusion injury rats in the EA group was decreased (<italic>P</italic>&lt;0.05).④TTC staining results showed that the area of brain infarction in the EA group and model group was respectively higher than that in the sham operation group, and the volume of brain infarction in the EA group was reduced compared with the model group (<italic>P</italic>&lt;0.05).④Compared with the model group, the levels of Beclin 1, LC3Ⅰ/Ⅱand PI3K mRNA and protein in the left side of hippocampus in the EA group were increased (<italic>P</italic>&lt;0.05).Conclusion:EA at the DU24 and DU20 acupoints can reduce the cerebral infarction, and improve animal behaviors, and protect the neuronal injury. …”
Get full text

Antimicrobial Susceptibility Testing for Colistin: Extended Application of Novel Quantitative and Morphologic Assay Using Scanning Electron Microscopy by Omar Zmerli, Sara Bellali, Gabriel Haddad, Rim Iwaza, Akiko Hisada, Erino Matsumoto, Yusuke Ominami, Didier Raoult, Jacques Bou Khalil

“…Phosphotungstic Acid (PTA) staining was applied, followed by SEM imaging using Hitachi TM4000PlusII-Tabletop-SEM at ×2000, ×5000 and ×7000 magnifications. …”
Get full text

Laminin-5 γ2 Chain Expression Correlates with Unfavorable Prognosis in Colon Carcinomas by C. Lenander, J. K. Habermann, Å. Öst, B. Nilsson, H. Schimmelpenning, K. Tryggvason, G. Auer

“…Epithelial cells of normal mucosa were consistently negative for staining. In contrast, positive cytoplasmic staining was observed in 89 tumors (96%). …”
Get full text

Bone Marrow Mesenchymal Stem Cells Decrease the Expression of RANKL in Collagen-Induced Arthritis Rats via Reducing the Levels of IL-22 by Fang Li, Xin Li, Guiyan Liu, Chong Gao, Xiaofeng Li

“…The concentrations of IL-22 and RANKL in serum were detected by ELISA and those in synovial tissue of rats’ joints by immunohistochemical staining. In addition, the expression of RANKL mRNA was measured by RT-PCR in the fibroblast-like synoviocytes (FLSs), cultured with IL-22 in vitro, which were delivered from the joints of CIA rats treated with or without MSCs. …”
Get full text

Generalized Lymphadenopathy: Unusual Presentation of Prostate Adenocarcinoma by Bulent Cetin, Zeynep Cetin, Suleyman Buyukberber, Ipek Isık Gonul, Ilgin Sahiner, Ugur Coskun, Mustafa Benekli

“…A biopsy of the inguinal lymph node was compatible with adenocarcinoma, whose prostatic origin was shown by immunohistochemical staining with PSA. The origin of the primary tumor was confirmed by directed prostate biopsy. …”
Get full text

La place des habitants dans la patrimonialisation conflictuelle du logement social by Géraldine Djament-Tran

“…Rather, the promotion of heritage is based on the views of experts (“cité Renaudie” at Villetaneuse), whilst intercommunality promotes residential activism (as in the garden-suburb of Stains). Promotion of heritage is threatened in two ways: mobilization against the notion by residents experiencing terrible social problems (“Cité des Poètes” in Pierrefitte), or the departure of such people away from social housing.…”
Get full text

Helicobacter (Campylobacter) pylori and Acid Peptic Diseases by Sigmund Kradjen, Philip Sherman

“…Diagnostic approaches chat have been used include tissue stains, culture of stomach biopsy specimens, labelled-urea breath tests and serology. …”
Get full text

Rare Isolated Pectoralis Minor Tear from a Noncontact Injury: Case Report and Review of the Literature by Danica D. Vance, Usama Qayyum, Charles M. Jobin

“…These injuries are often mistaken for pectoralis major muscle stains and tears. Advanced imaging with MRI helps define the anatomic location and grade of injury. …”
Get full text

Detection of Spargana on Ptyas korros in Sidoarjo by Ryan Bagaskara, Bambang Sektiari Lukiswanto, Adi Prijo Rahardjo, Emmanuel Djoko Poetranto, Setiawan Koesdarto, Mufasirin Mufasirin

“…Identification of Spirometra sp. was done using a native method, Semichen Acetic Carmine, and HE staining. As a results, 8 (44.4%) of 18 samples showed positive infection with Spirometra sp. …”
Get full text

Tuberculosis of the Oral Cavity Affecting Alveolus: A Case Report by Bipin Kumar

“…Biopsy from the ulcer margin revealed histological features of necrotizing granulomatous lesion. Stain for acid fast bacilli was positive.…”
Get full text

Generalized actinobacillosis in a cow by Enver Beytut, Mehmet Tuzcu

“…Microscopical examination revealed that the granulomas were characterized by colonies of bacteria surrounded by radially arranged projections in the center of neutrophil leucocyles which were again surrounded by macrophages and giant cells of Langhans type with outer fibrous capsule including lymphocyte and plasma cells. The sections stained with Brown and Brenn, Ziehl-Neelsen and Gridley methods were positive for Gram-negative bacteria; negative for acid-fast bacilli and mycotic agents, respectively.…”
Get full text

Neuroprotective Effect of Crocin on Rats with Focal Cerebral Ischemia-Reperfusion Injury Based on TLR4/NF-κB Pathway by LI Xiaolei, ZHU Haisheng, MA Ruijuan, HU Ke, FENG Lina, WANG Xudong

“…After reperfution for 24 h, modified neurological severity score (mNSS) was used to evaluate the neurological deficits; triphenyltetrazolium chloride (TTC) staining method was used to evaluate cerebral infarction rate, and dry-wet method was used to evaluate brain water content; hematoxylin-eosin staining (HE) staining and TUNEL staining were used to observe pathological changes and apoptosis of cortical neurons; ELISA method was used to detect the level of inflammatory factors in brain tissue (TNF-α, IL-1β, IL-6, etc.); western blot was used to detect the expression of toll-like receptor 4 (TLR4), nuclear factor-κB p65 (NF-κB p65), p-NF-κB p65, IκBα, p-IκBα, Cleved Caspase-3, B-lymphoma-2 gene (Bcl-2), Bcl-2-related X protein (Bax).Results(1) Neurological function, cerebral infarction rate and cerebral water content: compared with the sham operation group, the mNSS score, cerebral infarction rate, cerebral water content in the model group increased significantly (<italic>P</italic>&lt;0.05); compared with the model group, the mNSS score, cerebral infarction rate, cerebral water content in the crocin group and the crocin + lipopolysaccharide group decreased significantly (<italic>P</italic>&lt;0.05); compared with the crocin group, the mNSS score, cerebral infarction rate, cerebral water content in the crocin + lipopolysaccharide group increased significantly (<italic>P</italic>&lt;0.05). (2) Pathological changes and apoptosis of cortical neurons: the cortical neurons in the sham operation group showed normal morphology and complete structure; the pathological changes of cortical neurons such as disordered arrangement, decreased number, vacuolar degeneration, hyperchromatic cytoplasmic pyknosis, unclear nuclear membrane boundary, inflammatory cell infiltration were observed in the model group; the pathological changes of cortical neurons in the crocin group were significantly improved. …”
Get full text

Identification and functional characterization of the MYB transcription factor GmMYBLJ in soybean leaf senescence by Guohua Bao, Xiao Xu, Jing Yang, Juanjuan Liu, Tianran Shi, Xiaoxuan Zhao, Xuyan Li, Shaomin Bian

“…Moreover, transgenic soybean plants overexpressing GmMYBLJ grew faster and exhibited accelerated senescence under salt stress. DAB staining analysis showed that GmMYBLJ induced ROS accumulation in soybean hairy roots and Arabidopsis leaves. …”
Get full text

Properties of new polycationic bacteriochlorin photosensitizers: cytotoxicity and interaction with biofilms by Irina Tiganova, Yulia Zhizhimova, Eteri Tolordava, Elena Makarova, Natalia Shevlyagina, Alexander Barmashov, Maria Baryshnikova, Vladimir Zhukhovitsky, Yulia Romanova

“…The interaction of PS with the matrix was studied with electron microscopy, alcian blue staining, and quantitative evaluation of polysaccharides. …”
Get full text

Clinicopathological risk factors of oral second primary tumours by Jelena Karan, Miriam P. Rosin, Lewei Zhang, Denise M. Laronde

“…Positive toluidine blue staining indicated a trend toward higher risk of SPTs, whereas fluorescence visualization did not. …”
Get full text

Prognostic significance of mast cell and microvascular densities in malignant peripheral nerve sheath tumor with and without neurofibromatosis type 1 by Roberto André Torres de Vasconcelos, Pedro Guimarães Coscarelli, Thyago Marsicano Vieira, Washington Silva Noguera, Davy Carlos Mendes Rapozo, Marcus André Acioly

“…A secondary aim was to correlate histological staining to clinical data and survival in patients with and without NF1. …”
Get full text

Association of Structural Maintenance of Chromosome-1A Phosphorylation with Progression of Breast Cancer by Sushma Yadav, Claudia M. Kowolik, Daniel Schmolze, Yuan Yuan, Min Lin, Arthur D. Riggs, David A. Horne

“…Our results revealed that p-SMC1A staining intensity was lower in normal breast tissues compared to invasive or metastatic breast cancer tissues (<i>p</i> < 0.001). …”
Get full text

Akt/p27kip1 Pathway Is Not Involved in Human Insulinoma Tumorigenesis by Adriana Graciela Díaz, Andrea Paes de Lima, Paula Garibaldi, Maria de los Milagros Rubio, Florencia García, Marta Kral, Oscar D. Bruno

“…Cytoplasmic pp27kip1 staining was higher in insulinomas and surprisingly nearly half of the insulinomas also presented nuclear p27kip1 (p=0.029). …”
Get full text

Antitumor activity of edible fishes (Channa striata and Anabas testudineus) and gastropods (Helix aspersa and Pila virens) rudimentary mucus against HT-29 cell line and its biochem... by Elumalai Devan, Hemavathi Maduraiveeran, Kavitha Raja, Arulvasu Chinnasamy, Govindan Sivalingam, Sathya Balaji, Vinoth Neelakandan, Sharon Agnita, Murugan Rajaram

“…Propidium iodide staining showed nuclear damage followed by DNA damage. …”
Get full text