Design, Synthesis, and Biological Evaluation of Novel Pyrrolo [2,3-b] Pyridine Derivatives for Nonalcoholic Fatty Liver Disease by Jialin Ma, Jing Li, Dongping Yao, Qi Wang, Xinzhou Chen, Feiyan Tao, Bin Chen, Yongmei Xie, Lang Bai, Yiwen Zhang

“…Importantly, intraperitoneal administration of F-9 at 10 mg·kg−1·day−1 for 5 weeks had the most excellent effects to reduce the weight of the body, liver, and fat, as well as adjusting serum levels of AST, ALT, TC, TG, HDL, and LDL. H&E staining showed that F-9 could suppress fat deposition and increase the adipocyte size in the liver.…”
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Protocol to study the role of medial entorhinal cortex-basolateral amygdala circuit in context-induced retrieval of morphine withdrawal memory in mice by Yali Fu, Ting Ye, Ming Chen, Bin Lai, Ping Zheng

“…Then, we detail procedures of immunofluorescence staining and image analysis.For complete details on the use and execution of this protocol, please refer to Fu et al.1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.…”
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Evaluation of New Film Based on Chitosan/Gold Nanocomposites on Antibacterial Property and Wound-Healing Efficacy by Kai Wang, Haosheng Wang, Su Pan, Chuan Fu, YuXin Chang, Hongru Li, Xiaoyu Yang, Zhiping Qi

“…Furthermore, through the CCK experiment, we found that AuNPs at a certain concentration can improve the adhesion and proliferation of cells, which has also been proved in animal experiments, including gross appearance, histological observations, and immunofluorescence staining. Such findings support the feasibility of using the film as a promising candidate for tissue engineering of skin in a near future.…”
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Undifferentiated Malignant Neoplasm Involving Parotid and Thyroid: Sampling and PAX8 Cross-Reactivity Can Obscure the Diagnosis of Lymphoma by Elizabeth W. Hubbard, Laurentia Nodit, Stuart Van Meter

“…This distinction can have importance in selection of subsequent clinical therapy and should be considered in choice of immunohistochemical stains for diagnostic purposes.…”
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Intraperitoneal Granulomas Unexpectedly Found during a Cesarean Delivery: A Late Complication of Dropped Gallstones by David A. Suarez-Zamora, Luis E. Barrera-Herrera, Ricardo Caceres-Mileo, Mauricio A. Palau-Lazaro

“…Histological examination demonstrated a bile-stained material and a foreign body-type granulomatous response without neoplastic tissue. …”
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Enamel Based Composite Layers Deposited on Titanium Substrate with Antifungal Activity by S. L. Iconaru, A. M. Prodan, C. S. Turculet, M. Beuran, R. V. Ghita, A. Costescu, A. Groza, M. C. Chifiriuc, P. Chapon, S. Gaiaschi, R. Hristu, G. A. Stanciu, R. Trusca, M. Ganciu, S. M. Raita, N. Vineticu, C. S. Ciobanu

“…The Candida albicans biofilm development on the obtained layers was examined after 24 h, 48 h, and 72 h by confocal laser scanning microscopy (CLSM) and scanning electron microscopy (SEM) after ethidium bromide staining. A significant inhibition of the fungal adherence and biofilm development was observed on Ti-Enamel layers. …”
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A Definitive Diagnosis of Primary Hodgkin Lymphoma on Endoscopic Biopsy Material Utilizing in-Depth Immunohistochemical Analysis by Garnet Horne, Shaun AC Medlicott, Adnan Mansoor, Johan Lategan, Raymond Lai, P Beck

“…A case of a gastroesophageal junction primary HL is presented that was diagnosed on endoscopic biopsy material with the assistance of the immunohistochemical stains PAX5 and MUM1 which verified the presence of Reed-Sternberg cells. …”
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A Neonate with CLOVES Syndrome by Dilek Sarici, Mustafa Ali Akin, Selim Kurtoglu, Filiz Tubas, Serdar Umit Sarici

“…We herein report a very rare case of CLOVES syndrome with the findings of lipomatous overgrowth in the cheek (facial asymmetry), vascular malformation (hemangiomas), epidermal nevi (large port wine stains), and skeletal abnormalities (widened first interdigital space, dystrophia in the nail of the first digit of the right foot, and bilateral hypertrophy of the first digits of the feet).…”
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Application of Autofluorescence Endoscopy for Colorectal Cancer Screening: Rationale and an Update by Hiroyuki Aihara, Hisao Tajiri, Takeshi Suzuki

“…Autofluorescence endoscopy (AFE) can detect slight alterations in their distribution and concentration during the colorectal carcinogenesis process and, thus facilitate noninvasive screening colonoscopies without the need for fluorescent substances or staining reagents to be administered. While detecting faint autofluorescence intensity by conventional fiberoptic endoscopy remains challenging, the latest AFE system with high-resolution videoendoscope capabilities enables such detection by using a false-color display algorithm. …”
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Performance of HPV E4 and p16INK4a biomarkers in predicting regression of cervical intraepithelial neoplasia grade 2 (CIN2): protocol for a historical cohort study by David Jenkins, Mark H Stoler, Anne Hammer, Torben Steiniche, Lone Kjeld Petersen, Patti E Gravitt, Rikke Kamp Damgaard, Maurits NC de Koning, Wim GV Quint, John Doorbar, Johnny Kahlert

“…Tissue samples from women included will be sectioned for p16INK4a and HPV E4 immunohistochemical staining in addition to conventional hematoxylin and eosin (H&E) staining. …”
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Verbascoside Inhibits the Activation of NLRP3 Inflammasome and Improves BV-2 Cell Inflammatory Damage by Wenjing HUANG, Fang CHENG, Min DU, Jiayi CHEN, Wei XIANG, Ke SHI, Xingliang XIE, Yanmei SHENG

“…Hoechst 33342/PI double staining was utilized to examine the effect of the treatments on cell pyroptosis. …”
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Staphylococcus aureus vesicles impair cutaneous wound healing through p38 MAPK-MerTK cleavage-mediated inhibition of macrophage efferocytosis by Jiaxin Ou, Kangxin Li, Hui Yuan, Shaohua Du, Tingting Wang, Qiannan Deng, Huimei Wu, Weiyan Zeng, Kui Cheng, Kutty Selva Nandakumar

“…Macrophage efferocytosis efficiency was assessed using flow cytometry and confocal microscopy, while efferocytosis at wound sites was analyzed through WB, FACS, and TUNEL staining. Hematoxylin and eosin (H&E) staining and wound size measurements were used to evaluate the wound healing process. …”
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Inhibition of id-1 reduces osteosarcoma growth and metastasis through mediation of snail by Rongbing Shu, Zhuanyi Yu, Jianmin Wu, Qiuxin Cheng, Zhihao Peng, Huaqiang Zhou, Min Zhao

“…Tumor growth and lung metastasis were observed using HE staining. The expression of Id-1, Snail, and EMT-related proteins in osteosarcoma and lung tissues from each group of mice was assessed using Western blot and immunohistochemistry. …”
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Electroacupuncture Pretreatment Elicits Tolerance to Cerebral Ischemia/Reperfusion through Inhibition of the GluN2B/m-Calpain/p38 MAPK Proapoptotic Pathway by Bao-yu Zhang, Guan-ran Wang, Wen-hua Ning, Jian Liu, Sha Yang, Yan Shen, Yang Wang, Meng-xiong Zhao, Li Li

“…Model preparation for MCAO/R or sham MCAO/R started 2 hours after the last pretreatment. 24 hours after model preparation, the Garcia neurobehavioral scoring criteria was used for the evaluation of neurological deficits, TTC for the measurement of infarct volume, TUNEL staining for determination of neural cell apoptosis at hippocampal CA1 area, and WB and double immunofluorescence staining for expression and the cellular localization of GluN2B and m-calpain and p38 MAPK. …”
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SUMO modified ETV1 promotes M2-polarized tumor-associated macrophage infiltration and cancer progression by facilitating CCL2 transcription in esophageal squamous cell carcinoma ce... by Tianci Han, Xiaoqi Guo, Junwei Xie, Wei Tong, Liang Zhang

“…Immunofluorescence double staining was used to detect the tumor-associated macrophage (TAM) infiltration. …”
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Study on the protective effect of naringin-regulated lncRNA GAS5 against mitochondrial apoptosis in osteoarthritic chondrocytes by LIN Qin, ZHU Yingkai, LIN Yanming, LAN Shujie, CHEN Changxing, FU Changlong

“…Magnetic resonance imaging and ultrasound were used to identify the success of modeling; histopathological changes in rat articular cartilage were assessed using toluidine blue and saffron O staining; changes in the levels of lncRNA GAS5 in rat cartilage tissues after naringin intervention were detected by Real-time PCR; Western blot detected Caspase-3, Bax, MMP-13 and Cyt-C in rat cartilage tissues. …”
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Nicotine-induced CHRNA5 activation modulates CES1 expression, impacting head and neck squamous cell carcinoma recurrence and metastasis via MEK/ERK pathway by Chen Feng, Wei Mao, Chenyang Yuan, Pin Dong, Yuying Liu

“…Tumor formation assays are performed in nude mice using sh-CHRNA5 Cal27 cells, followed by western blot and immunohistochemical staining. Additionally, laryngeal and hypopharyngeal cancer tissues are analyzed through immunohistochemistry. …”
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The Roles of Skin Fibroblasts at Local Acupoints in Chrono-Acupuncture by Nannan Liu, Zhengyu Zhao, Qizhi Zhou, Xiaohong Zhang, Yu Wang, Shiqi Huang, Dingjun Cai

“…After 7 days of treatments, the skin and subcutaneous tissue of rats’ acupoint area of “Zusanli” on the affected limb were taken and then stained by immunofluorescence double staining method to observe the expression of the fibroblast cytoskeleton F-actin and β-tubulin under the LSCM while using western blot to observe the expression of P38MAPK/P-P38MAPK. …”
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Changes in Intestinal Microbiota Are Associated with Islet Function in a Mouse Model of Dietary Vitamin A Deficiency by Yunting Zhou, Junming Zhou, Yumin Zhang, Jun Tang, Bo Sun, Wei Xu, Xiaohang Wang, Yang Chen, Zilin Sun

“…The glucose metabolic phenotypes of the mice were assessed using glucose tolerance tests and immunohistochemistry staining. Changes in intestinal microbiota were assessed using 16S gene sequencing. …”
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Effects and Mechanisms of Cutting Upper Thoracic Sympathetic Trunk on Ventricular Rate in Ambulatory Canines with Persistent Atrial Fibrillation by Jie Cai, Min Tang, Hao Liu, Shiao Ding, Rongxin Lu, Wei Wang, Nan Ma, Ju Mei, Zhaolei Jiang

“…Compared with the control group, both left stellate ganglion (LSG) and right stellate ganglion (RSG) of the experimental group caused neural remodeling characterized by decreased ganglionic cell density and reduced TH staining. TH-positive component was significantly decreased in the left atrium of the experimental group compared with the control group. …”
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