A light microscopic investigation on the histology and alpha-naphthyl acetate esterase (anae)- and acid phosphatase (acp-ase) positive lymphocyte localization in the hemal nodes of... by Nariste Kadıralieva, Emrah Sur, Tuğba Özaydın, Şamil Sefergil

“…After processing, tissue sections were taken and stained with Crossmon's trichrome stain. In frozen sections, alpha-naphthyl acetate esterase (ANAE) and acid phosphatase (ACP-ase) were demonstrated. …”
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Employing Polymer and Gel to Fabricate Scaffold-like Cancellous Orthopedic Screw: Polycaprolactone/Chitosan/Hydroxyapatite by AmirHossein Badami, Javad Esmaeili, Hasan Mirtalaie

“…The screws were assessed mechanically, physically, and biologically (cell viability, cell attachment, DAPI, ALP staining, and Alizarin Red staining), and in vivo (a rat subcutaneous implantation model). …”
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β-ecdysone/PLGA composite scaffolds promote skull defect healing in diabetic rat by Yicai Luo, Ziwei Wu, Yingjuan Zhang, Yang Qiao, Yinge Wei, Xuan Yan, Xiangyu Ma, Xianxian Huang, Xiaoxia Zhong, Zhimao Ye, Xinping Lu, Hongbing Liao

“…The CCK8 cytotoxicity assay and live/dead cell staining demonstrated that BMSCs survived and proliferated on the composite scaffold under both low-glucose and high-glucose conditions. …”
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Histological changes in clıick bursa fabricii during postembryonic period by Nazlı Kocaöz, İlhami Çelik, Sadettin Ünsal

“…Tissue sections taken at 6 pm in thickness were stained with Grossman's trichrorne, Pappenheim's panoptic and methyl green-pyronin staining methods. …”
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Effects of Aspirin on Odontogenesis of Human Dental Pulp Cells and TGF-β1 Liberation from Dentin In Vitro by V. Khampatee, C. Zhang, L. Chou

“…The mitochondrial activity of HDPCs was assessed using an MTT assay. Crystal violet staining and triton were used to evaluate cell proliferation rates. …”
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EGFR as a biomarker of smoking status and survival in oropharyngeal squamous cell carcinoma by Shanmugappiriya Sivarajah, Morris Kostiuk, Cameron Lindsay, Lakshmi Puttagunta, Daniel A. O’Connell, Jeffrey Harris, Hadi Seikaly, Vincent L. Biron

“…Retrospectively collected tumors were used to construct tissue microarrays (TMAs), which were stained with EGFR, p16, DAPI and Pan-cytokeratin, and digitally quantified. …”
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ULK1 methylation promotes TGF‐β1‐induced endometrial fibrosis via the FOXP1/DNMT1 axis by Yuhua Zeng, Qing Feng

“…Cell proliferation was detected by CCK‐8 and EdU staining. Transcriptional regulation relationships were detected by dual luciferase reporter gene and chromatin immunoprecipitation (ChIP) assay. …”
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Optical Imaging of Integrin αβ Expression with Near-Infrared Fluorescent RGD Dimer with Tetra(ethylene glycol) Linkers by Zhaofei Liu, Shuanglong Liu, Gang Niu, Fan Wang, Shuang Liu, Xiaoyuan Chen

“…Fluorescent dye–labeled E[PEG 4 -c(RGDfK)] 2 were subjected to in vitro cell staining, in vivo NIRF imaging, ex vivo NIRF imaging, and histologic studies. …”
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Inhibition of Synovial Macrophage Pyroptosis Alleviates Synovitis and Fibrosis in Knee Osteoarthritis by Li Zhang, Runlin Xing, Zhengquan Huang, Nongshan Zhang, Li Zhang, Xiaochen Li, Peimin Wang

“…Besides, rats treated with the specific caspase1 inhibitor Ac-YVAD-CMK showed less inflammatory reaction and fibrosis, not only in the expression of proinflammatory factors IL-1β, IL-18, and HMGB1 and fibrosis markers TGF-β, PLOD2, COL1A1, and TIMP1 but also in the observation of HE staining, Sirius Red staining, and the transverse diameters of the right knees. …”
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Exploring the mechanism of berberine treatment for atherosclerosis combined with non-alcoholic fatty liver disease based on bioinformatic and experimental study. by Shushu Wang, Kachun Lu, Liwen Lin, Gaijie Li, Yuxin Han, Zhichao Lin, Qingmin Chu, Kunsheng Wu, Peijian Liu, Guiting Zhou, Rui Peng, Chuanjin Luo

“…The efficacy and mechanism of action of BBR were verified using methods such as hematoxylin and eosin (HE) staining, Oil Red O staining, and real-time quantitative PCR (RTqPCR). …”
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Design and Mechanism Study of 6c, a Novel Artesunate Derivatives, for Anti-Hepatocellular Carcinoma by Xiong SS

“…The ability of 6c to induce apoptosis was detected by Annexin V-FITC/PI double staining and Hoechst 33342 staining experiment. The effect of 6c on cycle arrest was detected by flow cytometry. …”
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CAPE activates AMPK and Foxo3 signaling to induce growth inhibition and ferroptosis in triple-negative breast cancer. by Qilu Fang, Qichuan Fang, Rui Cheng, Tingting Feng, Wenxiu Xin

“…Western blot, TUNEL and Annexin V-FITC/PI staining methods were employed to assess cell apoptosis. …”
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Isolation, identification, and quantification of Prevotella intermedia and Prevotella nigrescens in subgingival plaque samples of periodontally healthy and chronic periodontitis pa... by Vijayalakshmi Sharadindu Kotrashetti, Kishore Gajanan Bhat, Vijay Mahadev Kumbar, Sachita Shrikant Naik

“…The slides were stained using a specific oligonucleotide probe for PI and PN and then subjected to streptavidin-biotinylated horseradish peroxidase complex. …”
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Retrospective Observational Study on Rebamipide Ophthalmic Suspension on Quality of Life of Dry Eye Disease Patients by Yuri Sakane, Masahiko Yamaguchi, Atsushi Shiraishi

“…In addition, the standard fluorescein staining score, the Schirmer I test score, and the tear breakup time (TBUT) score were analyzed. …”
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Deer antler velvet (Cervus elaphus sibiricus) promotes fracture healing via partial BMP2-Smad mediated osteoblast differentiation by Ji Hyang Gu, Min-Young Chae, Jeong June Choi, Thanh Chu Duc, Chang Gue Son, Eun-Jung Lee

“…Results CES administration led to earlier union of the fractured bones, supported by Safranin O staining of the fracture region, demonstrating significantly increased cartilage formation day on 7 and a rapidly decreased cartilage area due to callus formation day on 14. …”
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Notch Signaling Pathway Promotes Th17 Cell Differentiation and Participates in Thyroid Autoimmune Injury in Experimental Autoimmune Thyroiditis Mice by Hao Liu, Yiwen Li, Yujiao Zhu, Lei Ma, Haibo Xue

“…Experimental mice were randomly divided into a control group, an EAT-A group (porcine thyroid immunoglobulin- (pTg-) treated mice) and an EAT-B group (treated with the DAPT γ-secretase inhibitor before pTg). HE staining, IHC staining, flow cytometry, RT-qPCR, and ELISA were used to evaluate the degrees of thyroiditis, detect the percentage of Th17 cells and measure the expression of retinoic acid-related orphan receptor gamma t (RORγt), interleukin-17A (IL-17A), and the main components of the Notch signaling pathway. …”
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Three-dimensional measurement of the depth of invasion in oral squamous cell carcinoma samples using Lugol's iodine-enhanced micro-computed tomography: an original study by Jiaxin Yu, Zhouyu Gu, Lichan Wang, Qian Zhang, Yumei Pu, Qingang Hu, Chengwan Xia, Yuxin Wang

“…Methodology: In total, 50 in vitro OSCC samples from Nanjing Stomatological Hospital (July 2022 to January 2024) were subjected to micro-CT imaging with a slice thickness of 50 μm following 3% Lugol iodine staining for 12 h, followed by pathological examination and staining. …”
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Aurora kinase A expression in pleomorphic adenoma, adenoid cystic carcinoma, and mucoepidermoid carcinoma of salivary glands: an immunohistochemical study by Razieh Zare, Leila Izadi, Mario Alberto Alarcón-Sánchez, Masumeh Taghva, Mohammad Ali Ranjbar

“…In 44.1% of cases, cells had a weak staining score, 27.9% a moderate score and the rest (27.9%) a strong score (p = 0.64). …”
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The Efficacy of Clinical Tests to Diagnose Evaporative Dry Eye Disease Related to Meibomian Gland Dysfunction by Jerry R. Paugh, Tiffany Nguyen, Alan Sasai, Elaine Chen, Melinda Thomas De Jesus, Justin Kwan, Andrew Loc Nguyen, Marjan Farid, Sumit Garg, James V. Jester

“…The global tests of tear breakup time, staining, and symptoms all produced AUCs ≥ 0.70, representing acceptable discrimination. …”
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The Effect of Human Bone Marrow Mesenchymal Stem Cell-Derived Exosomes on Cartilage Repair in Rabbits by Hongwei Yang, Meng Cong, Weixiao Huang, Jin Chen, Min Zhang, Xiaosong Gu, Cheng Sun, Huilin Yang

“…Moreover, wound healing assay showed that hBMSC-EXOs accelerate cell migration in primary chondrocytes. JC-1 staining revealed that the mitochondrial membrane potential was enhanced by hBMSC-EXOs, indicating cell apoptosis was decreased in the presence of hBMSC-EXOs. …”
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