Liraglutide alleviates high-fat diet-induced kidney injury in mice by regulating the CaMKKβ/AMPK pathway by Yingli Xuan, Ting-ting Ding, Xiao-lei Mao, Shiqing Pang, Ruibin He, Li Qin, Jiang zi Yuan

“…Besides, hematoxylin–eosin staining and periodic acid-Schiff staining were used to observe the pathological changes of kidney tissue; immunohistochemistry, western blot, and real-time quantitative PCR to assess the calmodulin-dependent protein kinase kinase beta (CaMKKβ)/AMPK signaling pathway activation.Results Liraglutide significantly reduced serum lipid loading, improved kidney function, and relieved kidney histopathological damage and glycogen deposition in the mouse model of obesity-related kidney disease induced by HFD. …”
Get full text

Inhibition of the Ubiquitin-Activating Enzyme UBA1 Suppresses Diet-Induced Atherosclerosis in Apolipoprotein E-Knockout Mice by Jiawei Liao, Xiaolei Yang, Qiuyue Lin, Shuang Liu, Yunpeng Xie, Yunlong Xia, Hui-Hua Li

“…UBA1 expression, detected by immunohistochemical staining, was found to be significantly increased in the atherosclerotic plaques, which confirmed to be mainly derived from lesional CD68+ macrophages via immunofluorescence costaining. …”
Get full text

Curcumin and α/β-Adrenergic Antagonists Cotreatment Reverse Liver Cirrhosis in Hamsters: Participation of Nrf-2 and NF-κB by José Roberto Macías-Pérez, Bruno Jesús Vázquez-López, Martin Humberto Muñoz-Ortega, Liseth Rubí Aldaba-Muruato, Sandra Luz Martínez-Hernández, Esperanza Sánchez-Alemán, Javier Ventura-Juárez

“…However, the best effect was the combined effect of doxazosin, carvedilol, and curcumin, reversing liver fibrosis and decreasing the amount of collagen I (Sirius red stain) without affecting the morphology of hepatocytes (hematoxylin and eosin stain), showing normal hepatic function (glucose, albumin, AST, ALT, total bilirubin, and total proteins). …”
Get full text

Witraże dziecięcego „bycia w życiu” – dobrostan versus obrazy codzienności by Justyna Kusztal, Małgorzata Turczyk

“…This means attempting to describe a child’s stained glass window „being in life”, actual or only apparent participation in particular areas of child’s activity in recent years. …”
Get full text

Olfactory Ensheathing Cell Tumor Arising from the Olfactory Mucosa by Eriko Ogino-Nishimura, Takayuki Nakagawa, Yoshiki Mikami, Juichi Ito

“…Immunohistochemically constituent cells were diffusely positive for S-100 protein, but olfactory ensheathing cell tumor was diagnosed by negative staining for Leu7 (CD57). This case indicates that olfactory ensheathing cell tumor should be included in differential diagnoses for the olfactory cleft tumors.…”
Get full text

Dentinogenic Ghost Cell Tumor of the Peripheral Variant Mimicking Epulis by Uddipan Kumar, Hitesh Vij, Ruchieka Vij, Jitin Kharbanda, IN Aparna, Raghu Radhakrishnan

“…Van Gieson histochemical stain further confirmed the nature of dentinoid-like material. …”
Get full text

New Insights about Pulmonary Epithelioid Hemangioendothelioma: Review of the Literature and Two Case Reports by Romeu Duarte Mesquita, Marta Sousa, Carmen Trinidad, Eugénia Pinto, Iosu Antón Badiola

“…The diagnosis is made on the basis of histopathological findings and confirmed by positive immunohistochemistry staining. Although the prognostic factors for PEH have not yet been well established, a better prognosis is usually associated with the multinodular pattern. …”
Get full text

Influence of Polyphenol Extract from Evening Primrose (Oenothera Paradoxa) Seeds on Proliferation of Caco-2 Cells and on Expression, Synthesis and Activity of Matrix Metalloprotein... by Szewczyk Karolina, Lewandowska Urszula, Owczarek Katarzyna, Sosnowska Dorota, Gorlach Sylwia, Koziołkiewicz Maria, Hrabec Zbigniew, Hrabec Elżbieta

“…To assess the effect of evening primrose extract on Caco-2 cell proliferation, crystal violet staining and sulforhodamine B (SRB) assays were used whereas mRNA expression and activity of MMPs were evaluated by RT-PCR and gelatin zymography.…”
Get full text

Hyperplastic Dental Follicle: A Case Report and Literature Review by Ligia Buloto Schmitd, Diego Maurício Bravo-Calderón, Cleverson Teixeira Soares, Denise Tostes Oliveira

“…Microscopic analyses based on routine staining and immunohistochemistry were used to discuss the cells found in pericoronal follicle. …”
Get full text

Protocol for generating liver metastasis microtissues to decipher cellular interactions between metastatic intestinal cancer and liver tissue by Maria Lamprou, Ana Krotenberg Garcia, Saskia Jacoba Elisabeth Suijkerbuijk

“…We then detail procedures for immunofluorescence staining, mounting microtissues, and sorting cells.For complete details on the use and execution of this protocol, please refer to Krotenberg Garcia et al.1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.…”
Get full text

The Heart as a Site of Metastasis of Benign Metastasizing Leiomyoma: Case Report and Review of the Literature by Mariam Meddeb, Robert D. Chow, Randolph Whipps, Reyaz Haque

“…Immunohistochemical staining was positive for smooth muscle α-actin and desmin, as well as estrogen and progesterone receptors, consistent with the diagnosis of uterine leiomyoma.…”
Get full text

Synthesis and Cytotoxicity of Chalcones and 5-Deoxyflavonoids by Jing Zhang, Xin-Ling Fu, Nan Yang, Qiu-An Wang

“…The cytotoxicity bioassays of these chalcones and 5-deoxyflavonoids were screened using the sulforhodamine B (SRB) protein staining method, and the results showed that compounds 2, 4, 5, 6, 10, 15, and 19 exhibited moderate cytotoxicity against the cancer cell line of MDA-MB-231, U251, BGC-823, and B16 in comparison with control drugs (HCPT, Vincristine, and Taxol).…”
Get full text

Leiomyoma of the Cheek by Ricardo Roberto de Souza Fonseca, Mário Augusto Ramos Junior, Douglas Baruchi, Tabata Resque Beckmann Carvalho, Andresa Borges Soares, Silvio Augusto Fernandes de Menezes, João de Jesus Viana Pinheiro, Jorge Nazareno Ferreira Fadul

“…Surgical excision was the treatment of choice, and the diagnosis was based on histopathological and immunohistochemical stains, which were positive for actin and desmin and negative for AE1/AE3, CD34, and S100. …”
Get full text

Subsequent Development of Desmoid Tumor after a Resected Gastrointestinal Stromal Tumor by Areen Abdulelah Murshid, Hatim Q. Al-Maghraby

“…We discuss the two entities of Desmoid tumor and GIST by comparing their definitions, clinical presentations, histological features, immunohistochemistry stains, molecular pathogenesis, prognosis, and treatment. …”
Get full text

Intracytoplasmic Crystalline Inclusions in the Hepatocytes of an Antelope by Sanjeev Gumber, Eric Lombardini, Bruce Williams, Nobuko Wakamatsu

“…Histologically, multifocal to coalescing areas of many hepatocytes contained large cytoplasmic vacuoles filled with pale eosinophilic homogeneous material and rare fine basophilic granules. Von Kossa staining showed the presence of calcium within cytoplasm, mainly in the inclusions, of hepatocytes. …”
Get full text

Mixed Medullary-Follicular Carcinoma of the Thyroid by Maasumeh Tohidi, Gholamreza Pourbehi, Mohammad Bahmanyar, Seyed Sajjad Eghbali, Mohammadreza Kalantar Hormozi, Iraj Nabipour

“…Immunohistochemical staining was positive for chromogranin, calcitonin, and thyroglobulin in tumoral cells. …”
Get full text

Characterization of Thermo- and Detergent Stable Antigenic Glycosylated Cysteine Protease of Euphorbia nivulia Buch.-Ham. and Evaluation of Its Ecofriendly Applications by Shamkant B. Badgujar, Raghunath T. Mahajan

“…In addition, the stability (temperature and surfactants) and hydrolysis of blood stain data also revealed its application in detergent industries. …”
Get full text

Adrenal Schwannomas: Rare Tumor of the Retroperitoneum by Emanuele Grasso, Michele Simone

“…Diagnosis of a schwannoma is based on detection of spindle cells with Antoni A and Antoni B regions in histological sections and positive staining for S-100 protein by immunohistochemical analysis. …”
Get full text

An Atypical Presentation of Sporadic Jejunal Burkitt’s Lymphoma by Pratik Naik, James Wang, Michael J. Brazeau, Domingo Rosario

“…Biopsy results and immunohistochemical stains were consistent with Burkitt’s lymphoma. PET/CT scan revealed only jejunal involvement. …”
Get full text

Automated and Reproducible Detection of Vascular Endothelial Growth Factor (VEGF) in Renal Tissue Sections by Nayana Damiani Macedo, Aline Rodrigues Buzin, Isabela Bastos de Araujo, Breno Valentim Nogueira, Tadeu Uggere Andrade, Denise Coutinho Endringer, Dominik Lenz

“…Light microscopy images of renal tissue sections stained for VEGF were analyzed. Subsequently, machine learning classified the cells as VEGF+ and VEGF- cells. …”
Get full text