Effects of Ginsenoside Rg1 Regulating Wnt/β-Catenin Signaling on Neural Stem Cells to Delay Brain Senescence by Yue Xiang, Shun-he Wang, Lu Wang, Zi-ling Wang, Hui Yao, Lin-bo Chen, Ya-ping Wang

“…The following indicators were detected: (1) NSC culture identification, (2) the effect of LiCl on the proliferation and survival rate of NSCs, (3) the effect of ginsenoside Rg1 on the proliferation and survival of NSCs, (4) the growth of NSCs in each group observed by an optical microscope, (5) the cell cycle of each group detected by flow cytometry, (6) the proliferative ability of each group detected by BrdU, (7) the fluorescence intensity of Nestin and Sox2 of NSCs in each group observed by a fluorescence microscope, (8) the positive rate of senescence staining analyzed by SA-β-Gal staining, (9) the localization of β-catenin in NSCs observed by laser confocal microscopy, and (10) the changes of the Wnt/β-catenin pathway-related proteins in each group detected by Western blotting. …”
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Upregulated miR-206 Aggravates Deep Vein Thrombosis by Regulating GJA1-Mediated Autophagy of Endothelial Progenitor Cells by Yan Li, Jingping Ge, Yuanyuan Yin, Ruowen Yang, Jie Kong, Jianping Gu

“…DVT formation in vivo was observed through hematoxylin-eosin (HE) staining. The expression of thrombus resolution markers, CD34 molecule (CD34) and matrix metallopeptidase 2 (MMP2), in the thrombi was measured by immunofluorescence staining. …”
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Formononetin ameliorates polycystic ovary syndrome through suppressing NLRP3 inflammasome by Zhuo Liu, Rui-Han Wang, Ke-Hua Wang

“…Protein expression was detected via western blot and immunofluorescence staining in the DHEA-induced PCOS rat model and DHT-induced PCOS cell model. …”
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lncRNA NEAT1 Downregulation Ameliorates the Myocardial Infarction of Mice by Regulating the miR-582-5p/F2RL2 Axis by Zhenhua Wu, Yunpeng Bai, Yujuan Qi, Chao Chang, Yan Jiao, Yaobang Bai, Zhigang Guo

“…The cell viability, proliferation, and apoptosis were determined by CCK-8, EdU staining, and flow cytometry assays. The expressions of F2RL2, miR-582-5p, and nuclear paraspeckle assembly transcript 1 (NEAT1) in myocardial tissues and HCMs were quantified by qRT-PCR or Western blot. …”
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Rapamycin Alleviates 2,4,6-Trinitrobenzene Sulfonic Acid-Induced Colitis through Autophagy Induction and NF-κB Pathway Inhibition in Mice by Zhen Ni, Hao Li, Dong Mu, Juanni Hou, Xiaoyan Liu, Shanhong Tang, Shumei Zheng

“…The expressions of p65, ATG16L1 and LC3 were detected by western blot and immunohistochemistry staining. The monodansylcadaverine (MDC) staining and transmission electron microscopy were developed to study the autophagy in LPS-induced HT-29 cells. …”
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CircSPG21 ameliorates oxidative stress-induced senescence in nucleus pulposus-derived mesenchymal stem cells and mitigates intervertebral disc degeneration through the miR-217/SIRT... by Yongbo Zhang, Sheng Yang, Xuan You, Zhengguang Li, Liuyang chen, Rui Dai, Hua Sun, Liang Zhang

“…Interactions among circSPG21, miR-217, and the NAD-dependent protein deacetylase sirtuin-1 (SIRT1) were validated through dual-luciferase assays, RNA fluorescence in situ hybridization and RNA immune precipitation. β-Gal staining, EdU staining, Western blotting, JC-1 assays, cell cycle analysis, and quantitative reverse transcription PCR (RT‒qPCR) were used to examine the functions of these molecules in TBHP-induced senescent NPMSCs. …”
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A pharmacoinformatic approach for studying Atractylodes Lancea DC’s anticancer potential and control ROS-mediated apoptosis against prostate cancer cells by Chi-Hoon Ahn, Ji Soo Myong, Kazi Rejvee Ahmed, Md Ataur Rahman, Md. Maharub Hossain Fahim, Min Choi, Muntajin Rahman, Jinwon Choi, Kiryang Kim, Seungjoon Moon, Seungjoon Moon, Mohammed Dalli, Mohammed Dalli, Rony Abdi Syahputra, Sang-Won Shin, Abdel Halim Harrath, Moon Nyeo Park, Bonglee Kim, Hwa-Seung Yoo

“…Cell viability was assessed using the EZ-Cytotoxic kit, while colony formation assays and TUNEL staining were used to meas-ure proliferation and apoptosis, respectively. …”
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Remarkable Differences in Calcification between the Primary Tumor and Metastatic Lymph Nodes in a Patient with ALK-Positive Non-Small-Cell Lung Cancer by Keeya Sunata, Tetsuo Tani, Takahiko Ui, Hidehiro Irie, Yohei Funatsu, Hidefumi Koh

“…Immunohistochemical staining and fluorescent in situ hybridization detected an anaplastic lymphoma kinase (ALK) fusion. …”
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Intestinal Tuberculosis Presenting with Gastrointestinal Bleeding in Patient on Warfarin Therapy by Werimo Pascal Kuka, Joe Rakiro, Joseph Gatheru, Felix Riunga, Allan Rajula

“…We present a case report of an African woman who presented to a Kenyan hospital with lower gastrointestinal bleeding while on anticoagulation for valvular atrial fibrillation, and was diagnosed with intestinal tuberculosis after colonoscopy, biopsy, and positive staining for tuberculous bacilli. Conclusion. Intestinal tuberculosis causing gastrointestinal bleeding is rare but should be suspected in patients living in TB endemic regions.…”
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2013 Cotton Defoliation and Harvest Aid Guide by Ramon Leon, David L. Wright, Barry J. Brecke

“…The greatest losses in quality are caused by stain from poorly defoliated plants or regrowth and moisture from green tissue. …”
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2013 Cotton Defoliation and Harvest Aid Guide by Ramon Leon, David L. Wright, Barry J. Brecke

“…The greatest losses in quality are caused by stain from poorly defoliated plants or regrowth and moisture from green tissue. …”
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Molecular profiling of bladder cancer xenografts defines relevant molecular subtypes and provides a resource for biomarker discovery. by Sharada Mokkapati, Ganiraju Manyam, Alexis R. Steinmetz, Côme Tholomier, Alberto Martini, Woonyoung Choi, Bogdon Czerniak, Byron H Lee, Colin P Dinney, David J McConkey

“…Ki67 was assessed as a continuous percentage of positively stained cells. Morphological assessment of xenografts included H&E and α-SMA staining. …”
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Cyclopiazonic acid suppresses the function of Leydig cells in prepubertal male rats by disrupting mitofusin 1-mediated mitochondrial function by Hang Lin, Ming Su, He Zhu, Yang Yu, Jianmin Sang, Yiyan Wang, Qiqi Zhu, Yang Zhu, Xiaoheng Li, Xingwang Li, Ren-Shan Ge, Huitao Li

“…The qPCR and Western blotting analyses revealed significant alterations in the expression of genes and proteins pertinent to PLC function, such as Scarb1, Star, Cyp11a1, and Cyp17a1. Immunofluorescence staining further revealed a reduction in MFN1 expression following exposure to CPA. …”
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High serum CA19-9 predicts severe cholecystitis in calculous cholecystitis patients by Shouwen Wang, Dachen Zhou, Wanjin Chen, Qi Guo, Liujin Hou, Ruolin Wu, Wei Wang, Muhammad Annus Khan, Muhammad Ahmad, Fan Huang, Meijuan Zheng, Guobin Wang, Hongchuan Zhao, Xiaoping Geng, Xiaojun Yu

“…Higher expression of CA19-9 was found by immunohistochemical staining in gallbladder specimen and more autophagy pathway related genes enriched in high group. …”
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PD-L1 monoclonal antibody alleviated MI injury of left ventricular function via modulating CD47/SHP2/SIRPα/SYK/FcγR signalings in tumor associated macrophages by Deyou Kong, Yongzhen Chen, Yajuan Yin, Zhikun Liu, Fang Yang, Xiaohong Li, Dongxing Shen, Jun Zhang

“…The extent of myocardial infarction was evaluated by Masson staining. In vitro experiments involved dividing macrophages, treated with different inducers, into 8 groups. …”
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β-Hydroxybutyrate Protects Against Cisplatin-Induced Renal Damage via Regulating Ferroptosis by Ruixue Tian, Shuqin Tang, Jingyu Zhao, Yajie Hao, Limei Zhao, Xiutao Han, Xingru Wang, Lijun Zhang, Rongshan Li, Xiaoshuang Zhou

“…Furthermore, western blotting and immunohistochemical staining indicated that β-HB may prevent kidney injury by regulating the Camkk2-AMPK pathway. …”
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A novel technique of cryodenervation for murine vagus nerve: implications for acute lung inflammation by Di Wu, Ximing Liao, Jing Gao, Kun Wang, Wujian Xu, Feilong Wang, Zhixian Jin, Dandan Wu, Qiang Li, Wei Gao

“…We first estimated the feasibility, safety and effectiveness of the technique via haematoxylin-eosin staining, histochemistry or immunofluorescence staining and immunoblotting assay. …”
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Melatonin Alleviates LPS-Induced Pyroptotic Cell Death in Human Stem Cell-Derived Cardiomyocytes by Activating Autophagy by Ya Qiu, Yan Ma, Min Jiang, Sulei Li, Jibin Zhang, Haixu Chen, Mengqi Xu, Shan Gao, Lei Tian, Bo Tao, Yabin Wang, Dong Han, Feng Cao

“…The hiPSC-CMs treated with LPS exhibited considerably fewer acidic vesicles (as revealed by LAMP1 staining) and autophagosomes (as revealed by LC3-II staining); however, Mel reversed this outcome in a dose-dependent manner. …”
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Overexpression of OsDUF868.12 enhances salt tolerance in rice by Hao Chen, Jiale Wan, Jiali Zhu, Ziyi Wang, Caiyao Mao, Wanjing Xu, Juan Yang, Yijuan Kong, Xiaofei Zan, Rongjun Chen, Jianqing Zhu, Zhengjun Xu, Lihua Li, Lihua Li

“…Physiological and biochemical assessments, along with leaf staining, demonstrated that overexpression of OsDUF868.12 improved the activity against oxidative stress.under salt stress. …”
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Wenxin Granules Influence the TGFβ-P38/JNK MAPK Signaling Pathway and Attenuate the Collagen Deposition in the Left Ventricle of Myocardial Infarction Rats by Ya Huang, Aiming Wu, Lixia Lou, Dongmei Zhang, Bo Nie, Yizhou Zhao, Keke Liu, Mingjing Zhao, Hongcai Shang

“…The changes of cardiac hemodynamics were detected by the catheter method, morphological changes were observed by HE staining, and myocardial tissue collagen volume was counted by Immunohistochemistry combined with Masson staining, and the expression of inflammatory TGFβ-p38/JNK MAPK signal pathway markers was detected by Western blot. …”
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