Effect and Mechanism of Action of Pushing Method on Rats with Skeletal Muscle Overuse Injury based on Wnt/β-catenin Signaling Pathway by DUAN Miaomiao, HUANG Bo, RUAN Lei, MU Pangpang, CHEN Yiming, PENG Liang

“…The balance beam test was used to assess the motor function of the rats after skeletal muscle injury at 24 h after the end of modeling and 24 h after the end of the intervention, respectively; hematoxylin-eosin staining (HE) method was used to observe the pathological changes of the gastrocnemius muscle; EM-1400 transmission electron microscope was used to observe the ultrastructure of the skeletal muscle fibers; and fluorescence PCR was used to detect the myogenic regulatory factor myogenic differentiation antigen (Myogenic differentiation antigen (MyoD), myogenin (MyoG) and paired box 7 (Pax7) mRNA transcript levels were detected by fluorescence quantitative PCR; MyoD, MyoG, Pax7, Wnt3a, Axin2 and β-catenin protein expression levels were detected by Western blot. …”
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Effect of Xingbi Gel Nasal Drops on Rats with Allergic Rhinitis Based on the Nasal Mucosa-Hippocampus Neuroimmune Mechanism by FAN Xueqi, QIU Caixia, YAN Shuiping, ZHUANG Xiangli, AI Si, GUO Lihua, ZHENG Jian

“…The AR rat behavioral scoring criteria was used to evaluate rhinitis behavior of rats; Morris water maze test was used to evaluate learning and memory function of rats; enzyme-linked immunosorbent assay (ELISA) was used to detect serum levels of immunoglobulin E (IgE) and transforming growth factor-β1 (TGF-β1); hematoxylin-eosin (HE) staining was used to observe pathological changes of nasal mucosa and eosinophil (EOS) count; immunohistochemistry method was used to detect expression levels of non-receptor tyrosine kinases Fyn, substance P (SP), vasoactive intestinal peptide (VIP) and neuropeptide Y (NPY) in the hippocampus; and real-time polymerase chain reaction (RT-PCR) was used to detect mRNA transcription levels of Fyn, SP, VIP and NPY in the hippocampus.Results(1) Behavioral scores of rhinitis: compared with the normal group, behavioral scores of rhinitis were significantly higher in the model group, the traditional Chinese medicine group and the western medicine group before treatment (<italic>P</italic>&lt;0.05). …”
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Hesperidin enhances broiler growth performance by augmenting gastric acid secretion via the proton pump pathway by Yunfei Li, Mingyuan An, Shasha Wan, Yifan Li, Yusong Du, Yufei Zhao, Huimin Li, Qingzhen Zhong, Zewei Sun

“…Proventriculus and gizzard tissues were used to evaluate pepsinogen levels, perform hematoxylin-eosin (H&E) staining, conduct enzyme-linked immunosorbent assays (ELISAs) for key proton pump components, and assess proton pump activity. …”
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T Cell-Derived Apoptotic Extracellular Vesicles Ameliorate Bone Loss via CD39 and CD73-Mediated ATP Hydrolysis by Yang X, Zhou Y, Zhou F, Bao L, Wang Z, Li Z, Ding F, Kuang H, Liu H, Tan S, Qiu X, Jing H, Liu S, Ma D

“…Western blot and immunogold staining confirmed the enrichment of CD39 and CD73 on ApoEVs. …”
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Effect of integrin α5 on NLRP3 expression in periodontal ligament fibroblasts within an inflammatory microenvironment by DAI Jingyi, CAI Hongxuan, SI Weixing, ZHANG Zan, WANG Zhurui, LI Mengsen, TIAN Ya guang

“…On the 30th day, the left maxillary tissue of rats was used for Micro-CT, HE staining, and immunohistochemical detection. Results The CCK-8 assay showed that CM, 25 μg/mL ATN-161, and ATN-161 concentrations below 25 μg/mL had no significant effect on cell viability at 12 h and 24 h (<i>P &gt; </i>0.05). 50-CM and 25 μg/mL ATN-161 significantly inhibited cell viability at 48 h (<i>P &lt; </i>0.05). …”
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Reproductive hormone profiles and gametogenesis in female of giant gouramy (Osphronemus gouramy) by Gratiana E. Wijayanti, . Soeminto, Sorta B.I. Simanjuntak

“…Ovarian were weighted to examine gonado somatic index (GSI), and they were then be fixative by NBF solution, processed to histology using paraffin, and stained with haematoxyline-eosin.   Histology of ovarian was observed using a light microscope.  …”
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Regulatory Effect of Pyroptosis Related Genes Expression in Adipose Tissue of Aging Rats by Long-Time Weight-Bearing Running by GONG Lijing, TANG Shuning, YANG Luyao, SU Hao, NI Zhen, FU Pengyu

“…After the intervention, the body weight, body composition, perirenal and subcutaneous adipose tissue (p/sWAT) wet weight and blood lipids were measured; the morphology of adipocytes were observed by HE staining, and the cross-sectional area (CSA) of adipocyte was calculated; the relative expression level of ASC, NF-κB, IL-1β, GSDMD, NLRP3 and Caspase1 mRNA were tested by RT-PCR; the immunopositive expression of ASC, IL-1β, GSDMD, NLRP3 and Caspase1 were observed by Immunofluorescence, and the integral optical density (IOD) value was calculated.Results(1) After the intervention, the body weight of rats in the control group was higher than that in the baseline group, and the body weight of rats in the resistance training group was lower than that in the control group (<italic>P</italic>&lt;0.05); the body fat percentage of the resistance training group was lower than those in the control and baseline groups (<italic>P</italic>&lt;0.05), respectively; the muscle content percentage of rats in the control group was lower than that in the baseline group, and the muscle content percentage of rats in the resistance training group was higher than that in the control group (<italic>P</italic>&lt;0.05). (2) After 32 weeks of intervention, the plasma levels of TG, TC, HDL-C, LDL-C and FFA of rats in the control group were higher than those in the baseline group, and the levels of TG and FFA of rats in the resistance training group were lower than those in the control group (<italic>P</italic>&lt;0.05). (3) After 32 weeks of intervention, the wet weight percentages of pWAT and sWAT of rats in the control group were higher than those in the baseline group; the wet weight percentage of pWAT of rats in the resistance training group was lower than those in both the control and baseline groups, and the wet weight percentage of sWAT of rats was lower than that in the control group (<italic>P</italic>&lt;0.05); the CSA of adipocyte of sWAT and pWAT of rats in the resistance training group was smaller than those in the control and baseline groups (<italic>P</italic>&lt;0.05), respectively. (4) After 32 weeks of intervention, the relative expression levels of ASC, NF-κB, IL-1β, NLRP3 and Caspase1 mRNA in pWAT of rats in the control group were higher than those in the baseline group, and the relative expression levels of NF-κB and IL-1β mRNA in sWAT were higher than those in the baseline group (<italic>P</italic>&lt;0.05). …”
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