Effect of Ginkgolide B on Non-Alcoholic Fatty Liver Disease based on Notch Signaling Pathway by Hong YU, Junzi WU, Bo SONG, Jun LI

“…After intervention for four weeks, some indexs such as aspartate aminotransferase (AST), alanine aminotransferase (ALT), low-density lipoprotein cholesterol (LDL-C) and high-density lipoprotein cholesterol (HDL-C) were compared; the differences of transforming growth factor-β1(TGF-β1) and tumor necrosis factor-α(TNF-α) in liver and plasma were compared; HE staining method was used to observe liver histopathological changes; Real-time fluorescent quantitative PCR method and Western blot method were used to detect the protein and mRNA expression of Notch-3, Hes-1. …”
Get full text

Effects of Electroacupuncture Preconditioning at Baihui and Zusanli Acupoints on the Expression of Inflammatory Factors in Brain and Intestines of Rats with Cerebral Ischemia/Reper... by ZHANG Jiyao, TANG Qiang, ZHU Luwen

“…Before surgery for one day, after reperfusion for 12 h and after reperfusion for 1,3,7 days, the grip tester was used to measure the forelimb grasping strength; after reperfusion for 7 days, ELISA methods was used to determine the content of IL-17A and IL-10 in serum, Western blot method was used to detect the expression of IL-17A and IL-10 protein in ischemic cortex and colon tissue, immunohistochemistry method was used to calculate the average optical density (AOD) value of IL-17A and IL-10 in ischemic cortex and colon tissue, and HE staining method was used to observe the pathological change of ischemic cortex.Results① Forelimb grasping strength: compared with the S-con group, the forelimb grasping strength of rats in the M-con group after reperfusion for 12 h, 1 d, 3 d, 7 d was decreased significantly (<italic>P</italic>&lt;0.05); compared with the M-con group, the forelimb grasping strength of rats in the EP group was increased significantly (<italic>P</italic>&lt;0.05). ② Content of IL-17A and IL-10 in serum: after reperfusion for 7 days , compared with the S-con group, the content of serum IL-17A and IL-10 of rats in the M-con group were significantly increased (<italic>P</italic>&lt;0.05); compared with the M-con group, the content of serum IL-17A in the EP group were significantly decreased (<italic>P</italic>&lt;0.05), and IL-10 in the EP group were significantly increased (<italic>P</italic>&lt;0.05). ③ Expression of IL-17A and IL-10 protein: after reperfusion for 7 days, compared with the S-con group, the expression of IL-17A and IL-10 protein in ischemic cortex and colon tissue in the M-con group were significantly increased (<italic>P</italic>&lt;0.05); compared with the M-con group, the expression of IL-17A in the EP group was significantly decreased (<italic>P</italic>&lt;0.05), and the expression of IL-10 protein in the EP group was significantly increased (<italic>P</italic>&lt;0.05). ④ AOD value of IL-17A and IL-10: after reperfusion for 7 days, compared with the S-con group, the AOD value of IL-17A and IL-10 in ischemic cortex and colon tissue in the M-con group were significantly increased (<italic>P</italic>&lt;0.05); compared with the M-con group, the AOD value of IL-17A in the EP group was significantly decreased (<italic>P</italic>&lt;0.05), the AOD value of IL-10 in the EP group was significantly increased (<italic>P</italic>&lt;0.05). ⑤ Pathological change of ischemic cortex: after reperfusion for 7 days, compared with the M-con group, the pathological change of ischemic cortex in EP group was significantly decreased (<italic>P</italic>&lt;0.05).ConclusionElectroacupuncture preconditioning at Baihui and Zusanli acupoints can reduce the pathological damage degree of the ischemic cortex and intestinal-central inflammation, improve neurobehavioral function, which may be related to brain-gut protection.…”
Get full text

Evaluation of Simulated Weightlessness Model of Hindlimb Unloading Miniature Pigs and Their Tissue Damage by TU Yingxin, JI Yilan, WANG Fei, YANG Dongming, WANG Dongdong, SUN Zhixin, DAI Yuexin, WANG Yanji, KAN Guanghan, WU Bin, ZHAO Deming, YANG Lifeng

“…After the experiment, the miniature pigs were euthanized and tissue samples were collected for histopathological observation of the cardiovascular, skeletal and muscle systems HE and Masson staining. Statistical analysis was also conducted on the thickness of arterial vessels and the diameter of skeletal muscle fibers. …”
Get full text

Supplementation of Arjun (Terminalia arjuna) bark powder prevented oxidative stress and enhanced antioxidants in kidneys on isoproterenol-treated Swiss albino mice model by Rashedul Haque, Sajib Paul, Md. Tipu Sultan, Faizul Islam Chowdhury, Md. Kawser, Shariful Islam Nayan, S.M. Hafiz Hassan, Afsana Kabir Chowdhury, Raiyana Huda, Sauda Sumaya Dina, Sheikh Zahir Raihan

“…Ultimately, a histopathological examination was conducted on the kidney specimens, which were subjected to Hematoxylin and Eosin staining. Administration of isoproterenol led to cell infiltration in the kidney, while therapy with Arjun resulted in improved renal imaging. …”
Get full text

Comparison of the Accuracy of Four Malaria Diagnostic Methods in a High Transmission Setting in Coastal Cameroon by Marcel N. Moyeh, Innocent M. Ali, Dieudonné L. Njimoh, Akindeh M. Nji, Palmer M. Netongo, Marie S. Evehe, Barbara Atogho-Tiedeu, Stephen M. Ghogomu, Wilfred F. Mbacham

“…Venous blood from 320 participants was used to detect the presence of malaria parasite using SD Bioline™ mRDT and Giemsa stained microscopy or spotted on filter paper for PCR amplification of the 18s rRNA gene of Plasmodium sp following standard procedures. …”
Get full text

Role of BDNF-TrkB signaling in the improvement of motor function and neuroplasticity after ischemic stroke in rats by transcranial direct current stimulation by Yu Chen, Lin Mao, Qinxiang Zhou, Dingqun Bai, Yuhan Kong

“…Behavioral tests, the modified neurologic severity score(mNSS), and the Hoffman reflex / the M wave(Hmax/Mmax) ratio helped assess motor function and neurologic deficits. HE and Nissl staining helped observe the morphological changes and count of nerve cells. …”
Get full text

Kuiyangling Enema Alleviates Ulcerative Colitis Mice by Reducing Levels of Intestinal NETs and Promoting HuR/VDR Signaling by Zhang D, Zhu Z, He Z, Duan S, Yi Q, Qiu M, Dai X, Su G, Li K, Xu L, Liu D, Wu Y, Gao Y, Li R, Guo S

“…Measurements of colon length, spleen index, histopathological variances, subcellular structure observations, ROS content, and NET-related proteins (PAD4, MPO, citH3) were obtained through HE staining, electron microscopy, live imaging, and Western blotting assays. …”
Get full text

Mechanism of DMOC in Diabetes Mellitus with Liver Fibrosis by Zizhi XIAO, Hong SHI, Huanwen RAO, Lifen LUO

“…DCFH-DA assay was used to detect ROS content in rat blood;ELISA was used to detect TNF-α, IL-6, and TGF-β1 content in rat blood;Masson's trichrome stain method was used to observe the changes of fibrosis in liver tissue;immunohistochemical method was used to observe the changes of Fibronectin and IV-Collagen in liver tissue;Western Blot was used to observe protein expression of Smad2, Smad3, pSmad2, pSmad3, α-SMA, MMP1, MMP2 and MMP9 in liver tissue.Results:Testing changes of anti-oxidation, anti-inflammation and anti-liver fibrosis in blood: the content of ROS, TNF-α, IL-6 and TGF-β1 in the model intervention group were significantly lower than those in the model group(<italic>P</italic>&lt;0.01).Observe the changes of fibrosis in liver tissue: liver tissue was found along the area around the portal vein with obvious hepatocyte necrosis and infiltration of inflammatory cells. …”
Get full text

Mechanism of Delaying Cartilage Degeneration in Knee Osteoarthritis with Rongjin Niantong Recipe Based on lncRNA NEAT1 and Nrf2/ARE Pathway by FU Changlong, XIE Xinyu, QIU Zhiwei, HUANG Yanfeng, JIN Linglu, LI Xihai

“…After the intervention, all groups were sacrificed under anesthesia, and the right knee tissues were separated and collected; HE staining was used to observe the pathological changes; Western blot was used to detect the expression levels of Nrf2, heme oxygenase-1 (HO-1), NAD (P) H quinone oxidoreductase-1 (NQO-1), kelch like ECH-associated protein 1 (Keap1) and inducible nitric oxide synthase (iNOS) in cartilage; Real-time PCR was used to detect the expression of lncRNA NEAT1 in cartilage tissue.Results1) Pathological changes of cartilage tissue: the cartilage matrix was light red and uniform, with complete tissue structure and clear tissue margins of each layer in the blank group; in the model group, the chondrocytes grew in clusters with thinner tangent layer. …”
Get full text

Effect of Acupuncture on the Expression of Heme Oxygenase 1 and Inflammatory Factors in Rats with Intracerebral Hemorrhage by CHEN Qiuxin, KONG Ying, YU Tingting, ZHANG Yu, LIU Peng, ZHANG Xin

“…Hematoxylin-eosin (HE) staining was used to observe the pathological changes of brain tissue. …”
Get full text

Fhl1, a new spatially specific protein, regulates vein graft neointimal hyperplasia by Chaoqun Wang, Jiantao Chen, Zicong Feng, Bohao Jian, Suiqing Huang, Kangni Feng, Haoliang Liu, Zhuoming Zhou, Ziyin Ye, Jing Lu, Mengya Liang, Zhongkai Wu

“…Methods In this study, Sprague–Dawley (SD) rats were used to establish an external jugular vein transplantation model, and the NIH pathophysiological process was tracked across 11 time points (0–35 days) using various histological stains. Spatial transcriptomics was performed on normal veins and 19‐day grafts to explore gene expression in neointimal regions. …”
Get full text

Mechanism study of KCMF1 promoting proliferation and NF-κB signaling transduction in colorectal cancer cells by WU Zhibai, XU Guiqin, ZHANG Li, YANG Zhaojuan, LIU Yun, JIAO Kun, CHEN Zehong, XU Chen, ZUO You, ZHENG Ningqian, YE Zhiqian, LIU Yongzhong

“…Furthermore, immunohistochemical staining was performed to detect the protein level of KCMF1 in 90 paired human CRC tissues and adjacent non-tumor tissues. …”
Get full text

Pengaruh Hiperglikemia terhadap Gambaran Histopatologis Pulau Langerhans Mencit by Muhammad Farid, Eryati Darwin, Delmi Sulastri

“…Morphometric analysis of islet of Langerhans on H&amp;E-stained pancreatic sections showed that the islet area and diameter were increased in group G1 (48607.13 μm2 and 240713.25 nm, respectively; p&lt;0.01) but decreased in group G2 (5471.42 μm2 and 81170.83 nm, p&lt;0.01) and G3 (4628.07 μm2 and 74730.86 nm, p&lt;0.05). …”
Get full text

Therapeutic Potential of <i>Cajanus cajan</i> (L.) <i>Millsp</i>. Leaf Extract in Modulating Gut Microbiota and Immune Response for the Treatment of Inflammatory Bowel Disease by Mingzhang Lin, Linghua Piao, Zhendong Zhao, Li Liao, Dayong Wang, Haiwen Zhang, Xiande Liu

“…The body weight, colon length, disease activity index (DAI), and histopathological examination using hematoxylin and eosin (H&E) staining were performed in the IBD model. The levels of the main inflammatory factors, specifically TNF-α, IL-1β, IL-6, and myeloperoxidase (MPO), were quantified by employing enzyme-linked immunosorbent assay (ELISA) kits. …”
Get full text

Genetic characteristics involving the PD-1/PD-L1/L2 and CD73/A2aR axes and the immunosuppressive microenvironment in DLBCL by Tingting Zhang, Zhenzhen Zhang, Xiubao Ren, Jin He, Zhengzi Qian, Bin Meng, Lei Jiao, Hengqi Liu, Lanfang Li, Lihua Qiu, Shiyong Zhou, Wenchen Gong, Huilai Zhang, Xianhuo Wang

“…In addition, multiplex immunofluorescence staining was used to quantitatively assess the expression of dysfunctional CD8+ T cells in DLBCL.Results SP140 was identified as a novel translocation partner for PD-L1, and a new inversion was detected between PD-L1 and PD-L2, both leading to the upregulation of PD-L1 expression. …”
Get full text

EZH2/miR-142-3p/HMGB1 axis mediates chondrocyte pyroptosis by regulating endoplasmic reticulum stress in knee osteoarthritis by Yang Chen, Shanshan Dong, Xin Zeng, Qing Xu, Mingwei Liang, Guangneng Liao, Lan Li, Bin Shen, Yanrong Lu, Haibo Si, Peifang Wei

“…The expression of the histone methyltransferase enhancer of zeste homolog 2 (EZH2), microRNA-142-3p (miR-142-3p), and high mobility group box 1 (HMGB1) and the levels of ER stress, pyroptosis, and metabolic markers in normal and OA chondrocytes were investigated by western blotting, quantitative polymerase chain reaction, immunohistochemistry, fluorescence in situ hybridization, fluorescein amidite-tyrosine-valine-alanine-aspartic acid-fluoromethyl ketone (FAM-YVAD-FMK)/Hoechst 33342/propidium iodide (PI) staining, lactate dehydrogenase (LDH) release assays, and cell viability assessments. …”
Get full text

LncRNA LUCAT1 offers protection against human coronary artery endothelial cellular oxidative stress injury through modulating hsa-miR-6776-5p/LRRC25 axis and activating autophagy f... by Yanjun Wang, Xin Zhang, Mengmeng Ren, Shu He, Hengjie Bie, Mengyang Duan, Zhiyuan Chen, Qiaowei Jia, Boyu Chi, Xiongkang Gan, Chengcheng Li, Yahong Fu, Hanxiao Zhou, Sheng Zhang, Qian Zhang, Fenghui An, Xiumei Chen, Enzhi Jia

“…Methods (1) Autophagy-related proteins including LC3, p62, Beclin1, ATG5, and ATG7 were immunohistochemical stained in coronary specimens; (2) The levels and function of autophagy in the HCAEC oxidative stress model were evaluated using western blot (WB), transmission electron microscopy (TEM), and mRFP-GFP-LC3 adenovirus transfection experiments; (3) The competing endogenous RNA (ceRNA) network of lncRNA LUCAT1/hsa-miR-6776-5p/LRRC25 axis was constructed and validated; (4) The expression levels of above autophagy-related RNAs in peripheral blood mononuclear cells (PBMCs) were verified by qPCR, and their diagnostic performance was subsequently analyzed using receiver operating characteristic (ROC) analysis. …”
Get full text

Clinicopathological and molecular characterization of astrocytoma by Xiaoyan Wu, Wenfeng Peng, Xu Zhang, Tao Tang, Ling Deng, Yuxia Xu, Xiaoyun Liu, Fang Wang, Wujian Peng, Jianrong Huang, Xiaoni Zhong

“…Immunohistochemical staining revealed that all four patients expressed OLIG2, EMA, and vimentin, while three patients also expressed GFAP and S-100. …”
Get full text

Protective mechanism of safflower yellow injection on myocardial ischemia-reperfusion injury in rats by activating NLRP3 inflammasome by Lingmei Li, Ce Cao, Hao Guo, Li Lin, Lei Li, Yehao Zhang, Gaojie Xin, Zixin Liu, Shujuan Xu, Xiao Han, Qiong Zhang, Jianhua Fu

“…The area of myocardial ischemia infarction and pathological changes were observed by the Tetrazolium method (TTC) and HE staining. Myocardial enzymes such as aspartate aminotransferase (AST), lactate dehydrogenase (LDH) and creatine kinase (CK) were measured by chemiluminescence (CL) method. …”
Get full text

Extended HPV genotyping by the BD Onclarity assay: concordance with screening HPV-DNA assays, triage biomarkers, and histopathology in women from the NTCC2 study by Laura De Marco, Simonetta Bisanzi, Guglielmo Ronco, Pamela Mancuso, Francesca Carozzi, Elena Allia, Raffaella Rizzolo, Daniela Gustinucci, Helena Frayle, Jessica Viti, Anna Iossa, Elena Cesarini, Simonetta Bulletti, Basilio Passamonti, Silvia Gori, Laura Toniolo, Francesco Venturelli, Annarosa Del Mistro, Paolo Giorgi Rossi, Maria Benevolo

“…Among the baseline-positive cases, we observed an increasing trend of BD positivity with cytology severity (from 71.6% in negative for intraepithelial lesion of malignancy to 95.1% in ASC-H+ samples), with histologically confirmed CIN3 (96.9%), with p16/ki67 dual staining positivity (90.9% among the positive and 69.6% among the negative specimens), and with E6/E7 mRNA positivity (93.4% in the mRNA-positive cases vs 39.7% among the mRNA-negatives). …”
Get full text