Histamine N-methyltransferase (HNMT) as a potential auxiliary biomarker for predicting adaptability to anti-HER2 drug treatment in breast cancer patients by Tzu-Chun Cheng, Mien-Chie Hung, Lu-Hai Wang, Shih-Hsin Tu, Chih-Hsiung Wu, Yun Yen, Chi-Long Chen, Jacqueline Whang-Peng, Wen-Jui Lee, You-Cheng Liao, Yu-Ching Lee, Min-Hsiung Pan, Hui-Kuan Lin, Huey-En Tzeng, Peixuan Guo, Cheng-Ying Chu, Li-Ching Chen, Yuan-Soon Ho

“…We also observed the nuclear translocation of HNMT/HER2-ICD cells using fluorescent double staining and DeltaVision microscopy. Finally, ChIP sequencing was employed to identify target genes affected by the HNMT/HER2-ICD complex. …”
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New insights into the immunomodulatory potential of sialic acid on monocyte-derived dendritic cells by Zélia Silva, João Amorim Rabaça, Vanessa Luz, Rita Adubeiro Lourenço, Mariolina Salio, Alexandra Couto Oliveira, Pedro Bule, Sebastian Springer, Paula Alexandra Videira

“…However, the cytokine cocktail induced a more pronounced IL-12 production. SNA lectin staining analysis demonstrated that the sialic acid profile is significantly altered by sialidase treatment, but not by the cytokine cocktail, which causes only slight sialic acid upregulation. …”
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Elucidating the pharmacological foundations and mechanisms of the Sihai Shuyu formula in treating Graves’ disease through integrated serum metabolomics and network pharmacology wit... by Xiaoju Liu, Xingjia Li, Wenbin Huang, Yifan Cui, Fengyun Cheng, Guofang Chen, Guofang Chen, Xiaodong Mao, Chao Liu, Chao Liu, Shuhang Xu

“…Hematoxylin-Eosin (H&E) staining assessed the effects of these pharmacological interventions on thyroid gland tissues. …”
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Cellular senescence induced by cholesterol accumulation is mediated by lysosomal ABCA1 in APOE4 and AD by Shaowei Wang, Boyang Li, Jie Li, Zhiheng Cai, Cristelle Hugo, Yi Sun, Lu Qian, Julia TCW, Helena C. Chui, Dante Dikeman, Isaac Asante, Stan G. Louie, David A. Bennett, Zoe Arvanitakis, Alan T. Remaley, Bilal E. Kerman, Hussein N. Yassine

“…Immunofluorescence and immunoblotting analyses confirmed increased lipofuscin-stained lipids and ABCA1 expression in AD brains and an association with mTOR phosphorylation. …”
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Biliary atresia susceptibility gene EFEMP1 regulates extrahepatic bile duct elastic fiber formation and mechanics by Kapish Gupta, Jessica Llewellyn, Emilia Roberts, Chengyang Liu, Ali Naji, Richard K. Assoian, Rebecca G. Wells

“…Fibulin-3 is deposited in most tissues and Efemp1 null mice have decreased elastic fibers in visceral fascia; however, fibulin-3 does not have a role in the development of large elastic fibers and its overall function in the extrahepatic bile ducts remains unclear. Methods: We used staining and histology to define the amount and organization of key extracellular matrix components in the extrahepatic bile ducts. …”
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Single-cell profiling of SLC family transporters: uncovering the role of SLC7A1 in osteosarcoma by Yan Liao, Junkai Chen, Hao Yao, Ting Zheng, Jian Tu, Weidong Chen, ZeHao Guo, Yutong Zou, Lili Wen, Xianbiao Xie

“…Pseudotime and CellChat analyses were performed to investigate the relationship between SLC7A1, malignant phenotypes, and the immune microenvironment. CCK8 assays, EdU staining, colony formation assays, Transwell assays, and co-culture systems were used to assess the effects of SLC7A1 on cell proliferation, metastasis, and macrophage polarization. …”
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Chemotherapy-induced cellular senescence promotes stemness of aggressive B-cell non-Hodgkin’s lymphoma via CCR7/ARHGAP18/IKBα signaling activation by Yangyang Wang, Fan Wu, Yang Wan, Huiping Wang, Linhui Hu, Qianshan Tao, Zhimin Zhai, Jiyu Wang, Keke Huang, Anwen Ren, Jinlan Li, Liuying Yi, Yanjie Ruan, Zhixiang Wanyan, Chaohong Liu

“…Cell proliferation was measured with the Cell Counting Kit-8 assay, and senescent cells were detected using senescence-associated β-galactosidase staining. The stemness of cells was evaluated through colony and sphere formation assays. …”
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ALDH1L2 drives HCC progression through TAM polarization by Jiajun Li, Chi Zhang, Qingqing Zhou, Qinqin Long, Jiayi Chen, Lili Meng, Wei Tian, Yue Yang, Chao Ge, Yuting Su, Xi-Dai Long, Jun Wu, Hua Tian

“…Methods: Immunohistochemistry, western blotting, and immunofluorescence staining were used to evaluate ALDH1L2 expression in HCC samples (n = 90) and cell lines (n = 9). …”
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Exosomal mir-126-3p derived from endothelial cells induces ion channel dysfunction by targeting RGS3 signaling in cardiomyocytes: a novel mechanism in Takotsubo cardiomyopathy by Xuehui Fan, Guoqiang Yang, Yinuo Wang, Haojie Shi, Katja Nitschke, Katherine Sattler, Mohammad Abumayyaleh, Lukas Cyganek, Philipp Nuhn, Thomas Worst, Bin Liao, Gergana Dobreva, Daniel Duerschmied, Xiaobo Zhou, Ibrahim El-Battrawy, Ibrahim Akin

“…The immunofluorescence staining, flow cytometry, qPCR, single-cell contraction, intracellular calcium transients, patch-clamp, dual luciferase reporter assay and western blot were performed for the study. …”
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Therapeutic role of miR-26a on cardiorenal injury in a mice model of angiotensin-II induced chronic kidney disease through inhibition of LIMS1/ILK pathway by Weijie Ni, Yajie Zhao, Jinxin Shen, Qing Yin, Yao Wang, Zuolin Li, Taotao Tang, Yi Wen, Yilin Zhang, Wei Jiang, Liangyunzi Jiang, Jinxuan Wei, Weihua Gan, Aiqing Zhang, Xiaoyu Zhou, Bin Wang, Bi-Cheng Liu, Yuanyuan Ji

“…In addition, Ang-II-treated AC16 cells and HK2 cells were used as in vitro models of cardiac and renal injury in the context of CKD. Histological staining, immunohistochemistry, quantitative real-time polymerase chain reaction (PCR), and Western blotting were applied to study the regulation of miR-26a on Ang-II-induced cardiac and renal injury. …”
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N-acetylcysteine increased the growth performance of Leuciscus waleckii by improving mitochondrial function, enhancing antioxidant and anti-inflammatory, and inhibiting apoptosis i... by Deng Lai Li, Rui Zhu, Si Tong Meng, Yin Tao Wang, Si Ying Liu, Zhi Yong Yang, Li Fang Wu

“…NAC supplementation upregulates the mRNA expression of Claudin-3, Claudin-f, ZO-1, and Occludin-a and reduces intestinal permeability-related markers, thereby maintaining intestinal physical barrier function. HE staining results showed that NAC maintained the morphological and structural integrity of the hepatopancreas and intestine. …”
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Polo-like Kinase 1 Expression as a Biomarker in Colorectal Cancer: A Retrospective Two-Center Study by Lana Jajac Brucic, Vesna Bisof, Majana Soce, Marko Skelin, Ivan Krecak, Andjela Nadinic, Branka Vrbicic, Zivana Puljiz, Suzana Hancic, Slavko Gasparov

“…Immunohistochemical staining was performed to assess PLK-1 expression. …”
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Effect and Mechanism of Running on Mouse Emphysema Model Induced by Cigarette Extract by LI Yongrong, XIE Haibin, LI hong, SUN Jie

“…Hematoxylin eosin (HE) staining was used to observe the pathological changes of lung tissue and evaluate the severity of emphysema in the four groups; the expression levels of inflammatory cells and cytokines in bronchoalveolar lavage fluid (BAL) and serum were analyzed to evaluate the pulmonary and systemic inflammation of the four groups; Western blot method was used to analyze the expression of STAT3 in lung tissues.Results1) Severity of emphysema: compared with the control group, the alveolar chord length of mice in the emphysema group and the emphysema + exercise group significantly increased, the difference was statistically significant (<italic>P</italic>&lt;0.05). …”
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Gambaran Slide Malaria Berdasarkan Sediaan Darah dari Kepulauan Siberut Mentawai Periode Oktober 2011 – Januari 2012 by Adeline Sacharissa Firdaus, Nuzulia Irawati, Arni Amir

“…Research done by microscopic examination of thick and thin blood preparations from peripheral blood samples that had been daubed with Giemsa staining to determine how the incidence, distribution of malaria by sex, distribution of malaria by species and Parasite Count. …”
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The compensatory enrichment of sphingosine-1-phosphate on HDL in FSGS enhances the protective function of glomerular endothelial cells compared to MCD by Yunfeng Xiong, Qiuping Ye, Lifang Liu, Wanjun Lin, Yonggen Liao, Ruiyu Gao, Jiaming Xu, Xinyu Zhang, Ruoyan Chen, Sihui Chen, Qiaoling Chen, Lixin Wei

“…Additionally, the synthesis activity of S1P in renal tissues of FSGS patients was markedly higher than that observed in MCD patients and healthy controls, suggesting that S1P may play a crucial protective role in the progression of FSGS. Immunofluorescence staining showed that compared with MCD and NC, the expression of S1PR1 in GECs of FSGS patients was significantly decreased. …”
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Gambaran Slide Malaria Berdasarkan Sediaan Darah dari Kepulauan Siberut Mentawai Periode Oktober 2011 – Januari 2012 by Adeline Sacharissa Firdaus, Nuzulia Irawati, Arni Amir

“…Research done by microscopic examination of thick and thin blood preparations from peripheral blood samples that had been daubed with Giemsa staining to determine how the incidence, distribution of malaria by sex, distribution of malaria by species and Parasite Count. …”
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Pengaruh Diet Tinggi Minyak Sawit Terhadap Sel Hepatosit Tikus by Susila Sastri, Husnil Kadri

“…After 4 weeks, all rats were slaughtered to take the livers, then the liver were preserved in formaldehyde for stained with haematoxylin eosin (HE) on the glass object. …”
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Optimization and Evaluation of Conditions for Orthotopic Nude Mouse Models of Human Liver Tumor Cells by MENG Yu, LIANG Dongli, ZHENG Linlin, ZHOU Yuanyuan, WANG Zhaoxia

“…On day 35 post-cell inoculation, mouse livers were dissected, and pathological slices were prepared for HE staining to observe histopathological changes in liver tissues. …”
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Investigating the Occurrence of Soil-Transmitted Parasites Contaminating Soil, Vegetables, and Green Fodder in the East of Nile Delta, Egypt by Samah H. Yahia, Samia E. Etewa, Abd Allah A. Al Hoot, Salwa Z. Arafa, Nesreen S. Saleh, Mohamed H. Sarhan, Suzan I. Rashad, Shimaa S. Hassan

“…Concentrations, including sedimentation, and flotation, and staining techniques were used to recover the greatest number of parasitic life forms. …”
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Prognostic significance of cell cycle-associated proteins p16, pRB, cyclin D1 and p53 in resected oropharyngeal carcinoma by Michaela Plath, Martina A. Broglie, Diana Förbs, Sandro J. Stoeckli, Wolfram Jochum

“…Methods Tissue microarray cores of 313 surgically treated OPSCC were stained for p16INK4a, pRB, cyclin D1 and p53 using immunohistochemistry. …”
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