Development of delayed pulmonary toxicities and transcriptional changes in pre-existing interstitial lung disease mice after partial thoracic irradiation by Jiamei Fu, Xinglong Liu, Yuchuan Zhou, Shengnan Zhao, Liang Zeng, Yan Pan, Jianghong Zhang, Kevin M. Prise, Chunlin Shao, Yaping Xu

“…Biopsy examination was performed on hematoxylin and eosin (H&E), Masson’s trichrome, and immunohistochemistry-stained lung tissue sections. Changes in lung function were measured. …”
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Rnd3 protects against doxorubicin-induced cardiotoxicity through inhibition of PANoptosis in a Rock1/Drp1/mitochondrial fission-dependent manner by Wen Ge, Xiaohua Zhang, Jie Lin, Yangyang Wang, Xiao Zhang, Yu Duan, Xinchun Dai, Jiye Zhang, Yan Zhang, Mengyuan Jiang, Huanhuan Qiang, Zhijing Zhao, Xuebin Zhang, Dongdong Sun

“…The molecular mechanism was explored through RNA sequencing, immunofluorescence staining, co-immunoprecipitation assay, and protein-protein docking. …”
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Fermented sugarcane juice-derived probiotic Levilactobacillus brevis RAMULAB54 enhances lipid metabolism and glucose homeostasis through PPAR-γ activation by V. B. Chandana Kumari, Ramith Ramu, Sujay S. Huligere, Shashank M. Patil, Shivasharanappa Nayakvadi, Sharath Bijoor, Uma Venkateswaran Manjappara, Mohammad Z. Ahmed, Ling Shing Wong

“…Organic acid interactions with PPAR-γ were evaluated via molecular docking, while cytotoxicity and adipocyte differentiation in 3T3-L1 cells were tested using MTT assays, Oil Red O staining, triglyceride quantification, and qRT-PCR. …”
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Three-dimensional structure of entire hydrated murine hearts at histological resolution by Jasper Frohn, Frederik Böddeker, Marius Reichardt, Hendrik Bruns, Titus Czajka, Amara Khan, Ludovic Broche, Michael Krisch, Alberto Bravin, Frauke Alves, Jana Zschüntzsch, Tim Salditt

“…It requires several invasive preparation steps, which introduce structural artefacts, namely dehydration, physical slicing and staining, or for the case of light sheet microscopy also clearing of the tissue. …”
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Comprehensive genomic and transcriptomic profiling of pulmonary nodules in synchronous multiple primary lung cancer by Xingsheng Liu, Kun Qian, Lei Su, Xiaoru Tian, Xin Zhao, Tengteng Wang, Li Han, Zhenzhen Li, Peilong Zhang, Ruotian Wang, Baodong Liu, Yuanbo Li, Xiaogang Tan, Yi Zhang

“…Additionally, the tumor tissues underwent histopathological evaluation through H&E staining, complemented by a comprehensive series of immunohistochemistry (IHC) analyses to detect protein expression. …”
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Recombinase-based amplification coupled with lateral flow chromatography for the specific and sensitive detection and identification of Leishmania major in cutaneous leishmaniasis... by Insaf Bel Hadj Ali, Yusr Saadi-Ben Aoun, Imen Khammeri, Hejer Souguir, Emna Harigua-Souiai, Hamed Chouaieb, Ahmed S. Chakroun, Meryem Lemrani, Aicha Kallel, Kalthoum Kallel, Nabil Haddad, Oussaima El Dbouni, Rhea N. Coler, Rhea N. Coler, Rhea N. Coler, Steven G. Reed, Akila Fathallah-Mili, Akila Fathallah-Mili, Ikram Guizani

“…Diagnosis is mainly based on microscopic direct examination (DE) of Giemsa-stained smears needing experienced microscopists. …”
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Goose astrovirus induces apoptosis and endoplasmic reticulum stress in gosling hepatocytes by Zhihua Lu, Haiqin Li, Xiaona Gao, Duanfeng Fu, Haoyu Huang, Cheng Huang, Meiqin Wu, Xiaoquan Guo

“…Hematoxylin and eosin (HE) staining revealed swollen and ruptured hepatocytes, with significant inflammatory cell infiltration. …”
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Gene expression changes in blastocyst hatching affect embryo implantation success in mice by Liyou An, Liang Zhang, Yulin Wu, Yadi Teng, Zihan Liu, Meixiang Ma, Miaolong Li, Xinrong Peng, Chenxi Liu

“…By immunofluorescence staining, we found C3 and IL-1β on the extra-luminal surface of the trophectoderm of the hatched blastocyst, suggesting that they play a role in maternal-fetal interactions. …”
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Colon Epithelial MicroRNA Network in Fatty Liver by Paule V. Joseph, Sarah K. Abey, Dan Wang, Nicolaas H. Fourie, Natnael D. Kenea, Tatyana G. Vishnyakova, Jeffrey M. Robinson, Kristen R. Weaver, Christina M. Boulineaux, Hannah R. Davidson, LeeAnne B. Sherwin, Onyinyechi Ozoji, Ana F. Diallo, Paul A. Smyser, Amy P. Patterson, Wendy A. Henderson

“…Changes in barrier (transepithelial electrical resistance, TEER) and epithelial cell junction structure (Occludin and Zona Occludens-1/ZO-1 immunofluorescence staining-confocal microscopy) were examined pre- and posttransfection in Caco-2 cell monolayers. …”
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E. Coli cytotoxic necrotizing factor-1 promotes colorectal carcinogenesis by causing oxidative stress, DNA damage and intestinal permeability alteration by Michela Tozzi, Alessia Fiore, Sara Travaglione, Francesca Marcon, Gabriella Rainaldi, Elena Angela Pia Germinario, Ilenia Laterza, Simona Donati, Daniele Macchia, Massimo Spada, Omar Leoni, Maria Cristina Quattrini, Donatella Pietraforte, Sofia Tomasoni, Filippo Torrigiani, Ranieri Verin, Paola Matarrese, Lucrezia Gambardella, Francesca Spadaro, Maria Carollo, Agostina Pietrantoni, Francesca Carlini, Concetta Panebianco, Valerio Pazienza, Filomena Colella, Donatella Lucchetti, Alessandro Sgambato, Antonella Sistigu, Federica Moschella, Marco Guidotti, Olimpia Vincentini, Zaira Maroccia, Mauro Biffoni, Roberta De Angelis, Laura Bracci, Alessia Fabbri

“…Immunohistochemistry and immunofluorescence stainings of formalin-fixed paraffin-embedded (FFPE) colon tissue were carried out as well as fecal microbiota composition analysis by 16 S rRNA gene sequencing. …”
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B7-H3 CAR-T cell therapy combined with irradiation is effective in targeting bulk and radiation-resistant chordoma cancer cells by Kun Wang, Gianpietro Dotti, Soldano Ferrone, Yufeng Wang, Yin P Hung, Tao Ma, Giulia Cattaneo, Xinhui Wang, David O Osei-Hwedieh, Tara A Walhart, Joseph H Schwab

“…The expression of B7-H3 in paraffin-embedded chordoma tissue was determined by immunohistochemical staining, and the expression of B7-H3 in chordoma cells was measured by flow cytometry. …”
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The Novel Histological Prostatic Inflammation Score Helps Defining the Association Between Stromal and Glandular Inflammation with the Risk of Prostate Cancer at Prostate Biopsy by Ugo Giovanni Falagario, Francesca Sanguedolce, Angelo Cormio, Antonella Ninivaggi, Marco Finati, Francesco Guzzi, Gian Maria Busetto, Carlo Bettocchi, Daniele Castellani, Giuseppe Carrieri, Luigi Cormio

“…<b>Methods:</b> Between January 2013 and December 2023, the Irani scores were routinely assessed on hematoxylin and eosin-stained PBx cores. The novel PIS was obtained by combining Irani G and A scores by their kernel distribution. …”
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NUAK1 acts as a novel regulator of PD-L1 via activating GSK-3β/β-catenin pathway in hepatocellular carcinoma by Chao-Yan Yao, Hang-Tian Tao, Jin-Jin He, Feng-Yi Zhu, Cui-Qing Xie, Yu-Na Cheng, Ji-Qin Li, Zhuang-Zhuang Liu, Chun-Yu Hou, Xue-Li Liu, Yong-Li Fan, Dong Fang, Xin-Rui Lv

“…Western blotting, immunofluorescence, real time PCR, and immunohistochemical staining were used to investigate the underlying mechanisms by which NUAK1 regulates PD-L1 expression in hepatocellular carcinoma. …”
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In-vitro and in-vivo assessment of biocompatibility and efficacy of ostrich eggshell membrane combined with platelet-rich plasma in Achilles tendon regeneration by Omid Koohi-Hosseinabadi, Reza Shahriarirad, Amireza Dehghanian, Laleh Amini, Sajjad Barzegar, Afrooz Daneshparvar, Omid Alavi, Seyedeh-Parvin Khazraei, Saba Hosseini, Ali Arabi Monfared, Roya Khorram, Nader Tanideh, Soheil Ashkani-Esfahani

“…In vitro biocompatibility was assessed using the MTT assay and DAPI staining, while in vivo biocompatibility was evaluated in a rat model. …”
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Molecular underpinnings of exceptional response in primary malignant melanoma of the esophagus to anti-PD-1 monotherapy by Yu Chen, Xuan Wang, Jie Dai, Li Zhou, Xuefeng Xia, Jun Guo, Xue Bai, Lu Si, Bixia Tang, Chuanliang Cui, Xiaoting Wei, Xinan Sheng, Siming Li, Zhihong Chi, Lili Mao, Bin Lian, Xieqiao Yan, Yan Kong, Caili Li, Xuan Gao, Lirui Tang, Linzi Sun, Zhonghui Qi, Zelong Xu

“…To explore the molecular mechanisms of the difference in therapeutic efficacy between PMME and NEMM, we performed genomic analysis, bulk RNA sequencing, and multiplex immunohistochemistry staining.Results We found that PMME (n=28) responded better to anti-PD-1 treatment than NEMM (n=64), with a significantly higher objective response rate (33.3% (95% CI 14.3% to 52.3%) vs 6.6% (95% CI 0.2% to 12.9%)) and disease control rate (74.1% (95% CI 56.4% to 91.7%) vs 37.7% (95% CI 25.2% to 50.2%)). …”
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Activated CD27+PD-1+ CD8 T Cells and CD4 T Regulatory Cells Dominate the Tumor Microenvironment in Refractory Celiac Disease Type II by Tessa Dieckman, Mette Schreurs, Ciska Lindelauf, Ahmed Mahfouz, Caroline R. Meijer, Louise Pigeaud, Vincent van Unen, Gerd Bouma, Frits Koning

“…We performed single-cell RNA-sequencing on CD45+ duodenal cells derived from a RCDII patient, immunofluorescence staining for in situ analysis and flow cytometry for phenotyping of RCDII aberrant and CD8 T cells. …”
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Reliability of radiomics features as imaging biomarkers for evaluating brain aging: A study based on myelin protein and diffusion tensor imaging by Yuting Yan, Mengmeng Hu, Xiaodong He, Yuyun Xu, Xiaojun Sun, Jiaxuan Peng, Fanfan Zhao, Yuan Shao

“…Myelin proteins, including proteolipid protein (PLP) and myelin basic protein (MBP), were examined by immunofluorescence staining in the regions of the whole brain, cortex, corpus callosum, striatum, and anterior commissure. …”
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Targeting of the G9a, DNMT1 and UHRF1 epigenetic complex as an effective strategy against pancreatic ductal adenocarcinoma by Daniel Oyon, Amaya Lopez-Pascual, Borja Castello-Uribe, Iker Uriarte, Giulia Orsi, Sofia Llorente, Jasmin Elurbide, Elena Adan-Villaescusa, Emiliana Valbuena-Goiricelaya, Ainara Irigaray-Miramon, Maria Ujue Latasa, Luz A. Martinez-Perez, Luca Reggiani Bonetti, Felipe Prosper, Mariano Ponz-Sarvise, Silvestre Vicent, Antonio Pineda-Lucena, David Ruiz-Clavijo, Bruno Sangro, Urko Aguirre Larracoechea, Tian V. Tian, Andrea Casadei-Gardini, Irene Amat, Maria Arechederra, Carmen Berasain, Jesus M. Urman, Matias A. Avila, Maite G. Fernandez-Barrena

“…Methods Immunohistochemical analysis of DNMT1, G9a, and UHRF1 expression was conducted in human PDAC tissue samples. Staining was semi-quantitatively scored, and overexpression was defined as moderate to strong positivity. …”
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Symptomatic Pituitary Metastasis as Initial Manifestation of Renal Cell Carcinoma: Case Report and Review of Literature by Gunjan Y. Gandhi, Russell Fung, Patrick E. Natter, Raafat Makary, K. C. Balaji

“…The diagnosis of renal cell carcinoma was confirmed by the diffuse and strong staining for renal cell carcinoma markers (Pax-8, RCC-1, and CD10). …”
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A novel synthetic synovial fluid model for investigating biofilm formation and antibiotic susceptibility in prosthetic joint infections by Amber De Bleeckere, Frits van Charante, Thibault Debord, Stien Vandendriessche, Michiel De Cock, Marte Verstraete, Fabien Lamret, Bram Lories, Jerina Boelens, Fany Reffuveille, Hans P. Steenackers, Tom Coenye

“…The biofilm preventing concentration (BPC) and minimal biofilm inhibitory concentration (MBIC) of relevant antibiotics were determined using a resazurin-based viability staining. BPC and MBIC values were compared to conventional susceptibility parameters (minimal inhibitory concentration and minimal bactericidal concentration) determined with conventional approaches. …”
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