Exosomal miR-222-3p derived from dermal papilla cells inhibits melanogenesis in melanocytes by targeting SOX10 in rabbits by Yang Chen, Tingting Lu, Yingying Dai, Yu Xue, Bohao Zhao, Xinsheng Wu

“…Methods DPCs and MCs were isolated from hair follicles of Rex rabbits and identified using western blotting (WB) and immunofluorescent staining. Exosomes derived from DPCs (DPCs-exos) were characterized using nanoparticle tracking analysis, transmission electron microscopy, and WB. …”
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Amantadine modulates novel macrophage phenotypes to enhance neural repair following spinal cord injury by Shijie Yang, Beibei Yu, Qing Zhang, Yongfeng Zhang, Longhui Fu, Bisheng Zhou, Haining Wu, Jianzhong Li, Shouping Gong

“…Subsequently, immunofluorescence staining, flow cytometry, and western blotting were performed to validate the identified effects of amantadine on macrophage differentiation and inflammation. …”
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Optimized Method to Generate Well-Characterized Macrophages from Induced Pluripotent Stem Cells by Qimin Hai, Peter Bazeley, Juying Han, Gregory Brubaker, Jennifer Powers, Claudia M. Diaz-Montero, Jonathan D. Smith

“…Using up to five independent iPSC donors, we performed several assays for macrophage functions and polarization, such as marker protein staining by flow cytometry, lipoprotein uptake, phagocytosis, cytokine release, inflammasome activation, and the effects of M1-like and M2-like polarization. …”
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Vinblastine resets tumor-associated macrophages toward M1 phenotype and promotes antitumor immune response by Jing Wang, Jin Wang, Yi-Na Wang, Yuan-Yuan Wang, Wen-Juan Bai, Nai-Jun Miao

“…However, no study to date has explored the effect of VBL on TAM shape changes and its role in tumor immune response.Method We used fluorescent staining of the cytoskeleton and quantitative analysis to reveal the morphological differences between M0, M1, M2, TAM and VBL-treated TAM. …”
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CX3CR1+ age-associated CD4+ T cells contribute to synovial inflammation in late-onset rheumatoid arthritis by Mitsuhiro Akiyama, Sohma Wakasugi, Keiko Yoshimoto, Koichi Saito, Sho Ishigaki, Risa Inukai, Yoshiyuki Matsuno, Waleed Alshehri, Yasushi Kondo, Yuko Kaneko

“…Additionally, immunohistochemical staining was performed on lymphoid node and synovial biopsy samples from patients with RA. …”
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A STUDY OF THE INFLUENCE OF EXTRACELLULAR VESICLES ON THE MORPHOFUNCTIONAL STATE OF THE ENDOMETRIUM IN VITRO by Оксана Александровна Мелкозерова, Ирина Викторовна Крутинь, Александра Юрьевна Храмцова, Мария Валерьевна Улитко, Алсу Фармутовна Мустафина

“…In the experimental groups, there were cells with up to 7 nuclei. When staining with fluorescent dyes separately on the preparation, which was microscopically examined 2 hours after applying 200 μl of vesicles, cells surrounded by an areola of small spherical formations were found, which are extracellular vesicles. …”
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Protective Effect of Rosmarinic Acid on Endotoxin-Induced Neuronal Damage Through Modulating GRP78/PERK/MANF Pathway by Li Q, Zheng JW, Wang ZY, Liao SP, Zhu L, Wang X, Wan LH

“…Brain injury was assessed using behavioral tests and hematoxylin and eosin (HE) staining. Western blotting and Immunohistochemistry (IHC) were performed to determine NeuN, GRP78, PERK, ATF6, IRE1α, and MANF expression levels. …”
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Heterogeneity of DNA Distribution in Diploid Cells: A New Predicitive Discriminant Factor for Solid Tumour Behaviour by Jacques Assailly, Arnaud Desgrippes, Brigitte Loridon‐Rosa, Dominique Piron, Roger Dachez, Daniel Beurton

“…Spatial nuclear DNA heterogeneity distribution of Feulgen‐stained DNA diploid cells was studied by image cytometry in voided urine of 119 patients without bladder tumour (n=20) and with initial (n=23) or previous (n=76) diagnosed bladder tumour. …”
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Blockades of effector T cell senescence and exhaustion synergistically enhance antitumor immunity and immunotherapy by Xia Liu, Emily Shaw, Fusheng Si, Guangyong Peng, Yan Tao, Feiya Ma, David Bagley, Yuanqin Zhang

“…Furthermore, T cell senescence induced by mouse tumor and regulatory T (Treg) cells in vitro was determined with multiple markers and assays, including real-time PCR, flow cytometry, and histochemistry staining. Loss-of-function strategies with pharmacological inhibitors and the knockout mouse model were used to identify the potential molecules and pathways involved in T cell senescence. …”
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Uncovering the role of tumor cGAS expression in predicting response to PD-1/L1 inhibitors in non-small cell lung cancer by Yuichi Ozawa, Yasuhiro Koh, Ryota Shibaki, Yuhei Harutani, Hiroaki Akamatsu, Atsushi Hayata, Takeya Sugimoto, Yuka Kitamura, Junya Fukuoka, Masanori Nakanishi, Nobuyuki Yamamoto

“…Methods In a post-hoc analysis of a prospective biomarker study with 106 advanced NSCLC patients treated with PD-1/L1 inhibitors from December 2015 to September 2018, tumor tissue before treatment from 68 patients was analyzed. cGAS and STING expression were measured using immunohistochemical staining and H-scores. Additionally, 40 serum proteins were quantified before and 4–6 weeks after treatment initiation. …”
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Medical treatment for dry eye in patients with keratoconus after corneal cross-linking by I.A. Bubnova, G.B. Egorova, V.V. Averich, T.S. Mitichkina

“…In a week after CXL, complete corneal epithelization without any signs of staining with fluorescein was observed in a total of 16 patients. …”
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Effect of bromelain on ischemia-reperfusion injury in the torsion model created in polycystic and normal ovarian tissues by Sevgi Ulusoy Tangul, Taylan Onat, Demet Aydoğan Kirmizi, Zuleyha Doganyigit, Emin Kaymak, Aslı Oflamaz, Atilla Şenayli, Salih Somuncu

“…After the procedure, tissues were collected for histopathological examination, and MDA, TUNEL, and NF-κB levels were measured.ResultsThis study detected significant decreases in MDA and NF-κB levels and apoptotic cell numbers assessed by TUNEL staining in groups with IR damage and given bromelain compared to the control groups. …”
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β-glucan attenuates intestinal ischemia-reperfusion injury in mice by promoting glucagon-like peptide-1 secretion by WANG Wei, HAN Ben, SUN Lihua

“…Methods‍　‍Male C57BL/6 mice (6~8 weeks old) were subjected, and finally, the experiments had sham group, II/R group, II/R+BG group (0.1 mg/mL BG in drinking water for 2 weeks before modeling), II/R+liraglutide (LLT, GLP-1 analogue) group (0.2 μg/g LLT injected every 12 hours for 3 consecutive days before modeling), and II/R+BG+Ex9-39 (GLP-1R antagonist) group (intraperitoneal injection of 2 μg/g Ex9-39 1 h before modeling). After modeling, HE staining was used to observe intestinal morphological changes, and RT-qPCR and Western blotting were employed to evaluate the molecules (Occludin, ZO-1 and Claudin-1) related to intestinal barrier damage. …”
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Ursodeoxycholic acid protects against sepsis-induced acute kidney injury by activating Nrf2/HO-1 and inhibiting NF-κB pathway by Yunpeng Lou, Hongguang Shi, Ning Sha, Feifei Li, Xiaofeng Gu, Huiyan Lin

“…Kidney injury was evaluated by HE staining and biochemical markers, including serum creatinine (Cr), blood urea nitrogen (BUN), urinary protein, neutrophil gelatinase-associated lipocalin (NGAL), kidney injury molecule-1 (KIM-1), N-acetyl-β-D-glucosaminidase (NAG), and retinol binding protein (RBP). …”
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The Upregulation of IL-1β Induced by Cisplatin Triggers PI3K/AKT/MMP9 Pathway in Pericytes Mediating the Leakage of the Blood Labyrinth Barrier by Yu M, Jiang WJ, Yu M, Zhou Z, Wang M, Li L

“…C57BL/6J mice were treated with CDDP and IL-1β levels, BLB permeability, and hearing thresholds were assessed using ELISA, histological staining, ABR test and BLB leakage tests. In vitro BLB models, the effect of IL-1β on MMP9 expression, PI3K-AKT pathway activation, and endothelial barrier permeability were examined via Western blot, TEER value test, and FITC extraction analysis. …”
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Activation of Delta-Opioid Receptor Protects ARPE19 Cells against Oxygen-Glucose Deprivation/Reoxygenation-Induced Necroptosis and Apoptosis by Inhibiting the Release of TNF-α by Runjie Guo, Ping Chen, Tiantian Fu, Ren Zhang, Yuan Zhu, Nange Jin, Hong Xu, Yong Xia, Xuesong Tian

“…Calcein acetoxymethylester/propidium iodide (Calcein-AM/PI) and Terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL) staining were used to detect necroptotic and apoptotic ARPE19 cells, respectively. …”
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Enhancement of host defense against Helicobacter pylori infection through modulation of the gastrointestinal microenvironment by Lactiplantibacillus plantarum Lp05 by Yao Dong, Mei Han, Yongmei Qi, Ying Wu, Zhipeng Zhou, Dacheng Jiang, Zhonghui Gai

“…Measurements included digestive enzymes (α-amylase, pepsin, cellulase), inflammatory markers (interleukin-17A, interleukin-23, interleukin-10, interferon-β, interferon-γ, FoxP3, endothelin, IP-10, TGF-β1), oxidative stress markers (catalase, malondialdehyde, superoxide dismutase, myeloperoxidase), and tissue pathology (via modified Warthin-Starry silver and H&E staining). Microbial community structure in the stomach and intestines was evaluated through 16S rRNA gene sequencing.ResultsIn vitro studies showed Lp05 and H. pylori formed co-aggregates, with Lp05 potentially disrupting H. pylori cell structure, reducing its stomach colonization. …”
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Molecular identification of Leptospira spp. in urine samples from female water buffaloes. Preliminary results by Rosaura Pérez-Gil, Héctor Nava-Trujillo

“…Additionally, hematocrit was determined by blood centrifugation in a capillary tube, and the presence of hemoparasites was determined by staining of blood smears. The DNA of Leptospira spp. was detected by PCR using two pairs of primers for the markers G1-G2, derived from a sequence from the genomic library of L. interrogans serovar icterohaemorragiae, strain RGA, which was described by Gravekamp et al. (1993). …”
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The Role of Immunohistochemistry for AMACR/p504s and p63 in Distinguishing Prostate Cancer from Benign Prostate Tissue Samples in Botswana by Ramatlho P, Rugemalila MM, Tawe L, Pain D, Choga OT, Ndlovu AK, Koobotse MO, Lal P, Rebbeck TR, Paganotti GM, Narasimhamurthy M, Kyokunda LT

“…Immunohistochemistry was performed using AMACR/p504s and p63 immunohistochemical stains.Results: The study found that AMACR/p504s had a sensitivity of 96% and a specificity of 95%, while p63 had a sensitivity and specificity of 100%. …”
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Lipopolysaccharide (LPS) Aggravates High Glucose- and Hypoxia/Reoxygenation-Induced Injury through Activating ROS-Dependent NLRP3 Inflammasome-Mediated Pyroptosis in H9C2 Cardiomyo... by Zhen Qiu, Yuhong He, Hao Ming, Shaoqing Lei, Yan Leng, Zhong-yuan Xia

“…The incidence of pyroptosis was detected by calcein-AM/propidium iodide (PI) double staining kit. The concentrations of IL-1β and IL-18 in the supernatants were assessed by ELISA. …”
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