A novel approach to digital characterisation of Tertiary Lymphoid Structures in colorectal cancer by Luis Munoz-Erazo, Luis Munoz-Erazo, Saem Mul Park, Saem Mul Park, Shelly Lin, Shelly Lin, Chun-Jen J. Chen, Chun-Jen J. Chen, Lisa Y. Y. Zhou, Lisa Y. Y. Zhou, Janet L. Rhodes, Janet L. Rhodes, Taesung Jeon, Sonya Fenton, John L. McCall, Roslyn A. Kemp, Roslyn A. Kemp, P. Rod Dunbar, P. Rod Dunbar

“…A recent three-dimensional reconstruction of TLS in CRC tissue showed that TLS are often large, multi-lobular structures, suggesting that assessing TLS across whole sections may be necessary to provide an accurate view of TLS activity in a patient’s tumour.MethodsWe used whole-section scans of multiplexed immunofluorescence images to characterise TLS from 22 subjects with CRC. Multiplexed staining for CD20, CD3, CD8, Foxp3 and Ki-67 enabled us to identify B-cells, CD8+ T-cells, Foxp3– CD4 T-cells, and Foxp3+ CD4 Tcells in all sections, and quantify both the presence of these cell subsets in lymphocytic clusters and their degree of proliferation within those clusters.ResultsIn total we identified 524 lymphocytic clusters with morphology consistent with TLS. …”
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Prognosis of nodal micrometastasis in resectable pN0 non-small cell lung cancer by Sophon Siwachat, Sophon Siwachat, Sophon Siwachat, Apichat Tantraworasin, Apichat Tantraworasin, Apichat Tantraworasin, Nirush Lertprasertsuke, Somcharoen Saeteng, Somcharoen Saeteng

“…However, the prognostic value of NMM in resectable NSCLC is still debated.MethodsThis retrospective cohort study at Maharaj Nakorn Chiang Mai Hospital from 2006 to 2017 assesses the prognostic impact of nodal micrometastasis in resectable pN0 NSCLC patients, using immunohistochemistry staining for cytokeratin AE1/AE3, p53, and BerEp4. …”
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Fatty acid synthase inhibition improves hypertension-induced erectile dysfunction by suppressing oxidative stress and NLRP3 inflammasome-dependent pyroptosis through activating the... by Jiaochen Luan, Mengchi Yu, Qi Gu, Xuan Zhou, Yunqiang Shao, Tong Chen, Jiayi Zhang, Zheng Zhu, Ninghong Song, Jie Yang

“…Molecular and protein expression were assessed using Western blotting, qRT-PCR, immunofluorescence staining, and immunohistochemistry.ResultsThe SHR exhibited ED, indicated by reduced maximum ICP/MAP ratios. …”
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Artificial intelligence-enabled discovery of a RIPK3 inhibitor with neuroprotective effects in an acute glaucoma mouse model by Xing Tu, Zixing Zou, Jiahui Li, Simiao Zeng, Zhengchao Luo, Gen Li, Yuanxu Gao, Kang Zhang, Jing Ni

“…Subsequently, these target candidates were validated using molecular simulations (molecular docking, absorption, distribution, metabolism, excretion, and toxicity [ADMET] prediction, and molecular dynamics simulations) and biological experiments (Western blotting and fluorescence staining) in vitro and in vivo. Results:. AI-driven drug screening techniques have the potential to greatly accelerate drug development. …”
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Targeted ErbB4 receptor activation prevents D-galactose-induced neuronal senescence via inhibiting ferroptosis pathway by Ji-Ji Dao, Ji-Ji Dao, Wei Zhang, Wei Zhang, Chong Liu, Chong Liu, Qian Li, Qian Li, Chen-Meng Qiao, Chen-Meng Qiao, Chun Cui, Chun Cui, Yan-Qin Shen, Yan-Qin Shen, Shuang-Xi Chen, Shuang-Xi Chen, Wei-Jiang Zhao, Wei-Jiang Zhao

“…Behavioral assessments were conducted to evaluate therapeutic efficacy in memory and cognitive functions. Immunofluorescence staining, western blot, and biochemical assays were primarily employed to assess changes in both senescence- and ferroptosis-related molecules in mouse hippocampal tissues in response to each treatment. …”
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Dihydrotanshinone I enhanced BRAF mutant melanoma treatment efficacy by inhibiting the STAT3/SOX2 signaling pathway by Xing Luo, Yi Duan, Jinwei He, CongGai Huang, Jun Liu, Yifan Liu, Mengdei Xu, Qiong Dai, Zhihui Yang

“…The diameter and weight of tumours in each group were then measured, and IHC and HE staining were used to assess the expression of two pathways and cytotoxicity, respectively.ResultsThis study found that DHT directly interacts with STAT3 protein and it can stop the feedback activation of the STAT3/SOX2 pathway caused by the use of MAPK pathway inhibitors. …”
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N6-methyladenosine methylation regulators can serve as potential biomarkers for endometriosis related infertility by Yalun He, Jie Ding, Tonglin Bai, Yangshuo Li, Xiaolan Liang, Yiming Chen, Yi Lin, Wen Cheng, Chaoqin Yu

“…Immunohistochemical staining confirmed that HNRNPA2B1 and HNRNPC expression levels were significantly higher in the eutopic endometrium of fertile women compared to ovarian EMS patients. …”
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Adrenal aldosterone synthase (CYP11B2) histopathology and its association with disease-induced sudden death: a cross-sectional studyResearch in context by Antero Ylänen, Juhani Isojärvi, Antti Virtanen, Helena Leijon, Tiina Vesterinen, Aapo L. Aro, Heini Huhtala, Eeva Kokko, Ilkka Pörsti, Marianna Viukari, Pasi I. Nevalainen, Niina Matikainen

“…Methods: In this study, autopsies and histopathological analyses, including aldosterone synthase staining of adrenal glands, were performed on 403 consecutive individuals who experienced sudden death. …”
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Sevoflurane Exacerbates Cognitive Impairment Induced by Aβ1–40 in Rats through Initiating Neurotoxicity, Neuroinflammation, and Neuronal Apoptosis in Rat Hippocampus by Yue Tian, Ke-yan Chen, Li-dan Liu, Yun-xia Dong, Ping Zhao, Shan-bin Guo

“…Protein and mRNA expressions were executed by immunohistochemical (IHC) staining, Western blotting, and qRT-PCR. Results. Compared with the NS-treated group, sevoflurane only caused cognitive impairment and increased the level of Aβ1–42 of the brain in the Aβ-treated group. …”
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Bacteriological Profile of Chronic Suppurative Otitis Media and its Antibiotic Sensitivity Pattern at a Tertiary Care Hospital by Geeta Geeta, Naveen G, Apoorva P, Harish MO, Arathi Nair, Harshitha MC, Kiran Revankar, Girish PB

“…Ear discharges were subjected for aerobic bacterial culture under aseptic conditions along with direct Gram staining. Antibiotic sensitivity testing was done using Kirby–Bauer disc diffusion method on Mueller– Hinton Agar. …”
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Comparative Analysis of the Occurrence and Role of CX3CL1 (Fractalkine) and Its Receptor CX3CR1 in Hemophilic Arthropathy and Osteoarthritis by Piotr Wojdasiewicz, Łukasz A. Poniatowski, Andrzej Kotela, Marta Skoda, Michał Pyzlak, Aleksandra Stangret, Ireneusz Kotela, Dariusz Szukiewicz

“…Tissue specimens including articular cartilage and synovial membrane were similarly collected during surgery and stained immunohistologically using selected antibodies including anti-CX3CR1, anti-CD56, anti-CD68, and anti-CD31. …”
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Increased nerve density adversely affects outcome in colorectal cancer and denervation suppresses tumor growth by Hao Wang, Ruixue Huo, Kexin He, Weihan Li, Yuan Gao, Wei He, Minhao Yu, Shu-Heng Jiang, Junli Xue

“…Methods Tissue samples from 129 CRC patients were stained with immunofluorescent markers NF-L and S100B to detect nerves. …”
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Eugenol inhibits NEAT1 as a ceRNA in pre-cancerous breast lesions by Yusheng Liu, Guijuan Zhang, Yi Ma, Min Ma, Xuefeng Jiang

“…Besides, Nuclear Paraspeckle Assembly Transcript 1 (NEAT1), miR-383-5p, miR-9-5p, matrix metalloproteinase-9 (MMP-9), and vascular endothelial growth factor-A (VEGF-A) expression was examined through quantitative reverse transcription polymerase chain reaction (RT-qPCR), Western blotting, and immunohistochemical staining analyses. Results: There were altogether 1162 mRNAs, 81 miRNAs, and 26 lncRNAs recognized as trend genes in breast cancer (BC) and pre-cancerous BC (pBC), constructing the ceRNA network using 3 lncRNAs, 3 miRNAs, and 38 mRNAs. …”
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IL-17 triggers PD-L1 gene transcription in NSCLC cells via TRIM31-dependent MEF2C K63-linked polyubiquitination by Shuai Ying, Ningxia Wu, Yuting Ruan, Wen Ge, Pei Ma, Tongpeng Xu, Yongqian Shu, Yingwei Wang, Wen Qiu, Chenhui Zhao

“…Co-IP/IB was used to examine the interaction between MEF2C and PD-L1, including MEF2C ubiquitination. IHC staining was carried out to analyse the expression of IL-17RA, MEF2C, TRIM31, and PD-L1 in NSCLC tissue arrays. …”
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The use of bacterial culture for Leptospirosis di- agnosis in female water buffaloes by Rosaura Pérez-Gil, Oriana Gavidia, Juan Torres

“…A portion of the tissue sample was fixed in 10% buffered formalin for histological slices and routine H-E staining. The MAT results indicated titers of 1:50 for the Icterohaem morragiae serovar at 5.5%, Pomona at 5.5%, and Bratislava at 5.5%. …”
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Molecular Characterization, Oxidative Stress-Mediated Genotoxicity, and Hemato-Biochemical Changes in Domestic Water Buffaloes Naturally Infected with <i>Trypanosoma evansi</i> Und... by Waqas Ahmad, Muhammad Yasin Tipu, Muti ur Rehman Khan, Haroon Akbar, Aftab Ahmad Anjum, Muhammad Ovais Omer

“…The impact of parasitemia loads was correlated with oxidative stress markers, genotoxicity, and hematobiochemical parameters using Pearson correlation and multivariable regression models. (3) Results: Field-stained thin blood film microscopy and molecular identification revealed 8.98% and 10.18% infection rates, respectively. …”
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Effect of Qing'e Formula on OPN, BSP and Integrin ɑ_vβ₃Expression in Bone Tissue of Ovariectomized Rats by Yuqing SUN, Wenjuan LUO, Bingqi WANG, Xiang CHEN, Xiaokang JIA, Yanping LIN

“…The bone tissues from the left proximal shin were sectioned and processed HE staining. The bone mineral densities (BMD) were measured by dual-energy X-ray absorptiometry (DXA) for the 3-5 lumbar vertebra. …”
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Neutrophil Extracellular Trap Components Associate with Infarct Size, Ventricular Function, and Clinical Outcome in STEMI by Ragnhild Helseth, Christian Shetelig, Geir Øystein Andersen, Miriam Sjåstad Langseth, Shanmuganathan Limalanathan, Trine B. Opstad, Harald Arnesen, Pavel Hoffmann, Jan Eritsland, Ingebjørg Seljeflot

“…Double-stranded deoxyribonucleic acid (dsDNA) and myeloperoxidase-DNA (MPO-DNA) were measured in serum by a nucleic acid stain and ELISA. Cardiac magnetic resonance imaging assessed microvascular obstruction (MVO), area at risk, infarct size, myocardial salvage index, left ventricular ejection fraction (LVEF), and change in indexed left ventricular end-diastolic volume (LVEDVi). …”
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Mechanism of Mongolian Medicine Batri-7 on Salmonella Enteritis by Yang D, Chen S, Borijihan H, Aoqier A, Sarula S, Siqin S, Manda M, Temuqile T, Baigude H

“…Pathological analysis of small intestine was conducted with hematoxylin and eosin staining. The macrophage recruitment in model mice’s intestines was detected by flow cytometry. …”
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Cellular Cholesterol Loss Impairs Synaptic Vesicle Mobility via the CAMK2/Synapsin-1 Signaling Pathway by Atikam Qurban, Mengqi Zhang, Hengbing Zu, Kai Yao

“…Filipin III cholesterol staining was employed to visualize membrane and intracellular cholesterol in the different cell models, and fluorescence intensity was assessed using confocal microscopy. …”
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