Transcriptome and DNA methylation profiling during the NSN to SN transition in mouse oocytes by Hannah Demond, Soumen Khan, Juan Castillo-Fernandez, Courtney W. Hanna, Gavin Kelsey

“…Results To study the transcriptome and DNA methylation dynamics during the NSN to SN transition, we used single-cell (sc)M&T-seq to generate scRNA-seq and sc-bisulphite-seq (scBS-seq) data from GV oocytes classified as NSN or SN by Hoechst staining of their nuclei. Transcriptome analysis showed a lower number of detected transcripts in SN compared with NSN oocytes as well as downregulation of 576 genes, which were enriched for processes related to mRNA processing. …”
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A preliminary study on the prognostic significance of cysteine-rich EGF ligand domain 2 protein (CRELD2) in patients with triple negative breast cancer by Mehmet Zahid Kocak, Murat Araz, Siddika Findik, Aykut Demirkiran, Mustafa Korkmaz, Melek Karakurt Eryilmaz, Mehmet Artac

“…CRELD2 protein expression in tumor tissue was determined by immunohistochemical staining (IHC). The patients were divided into two groups: CRELD2 positive and negative groups. …”
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Evaluation of cannabinoid type 2 receptor expression and pyridine-based radiotracers in brains from a mouse model of Alzheimer’s disease by Vasil Kecheliev, Francesco Spinelli, Adrienne Herde, Achi Haider, Linjing Mu, Linjing Mu, Jan Klohs, Simon M. Ametamey, Ruiqing Ni, Ruiqing Ni

“…Immunohistochemical staining for amyloid-ß deposits (6E10), microgliosis (anti-Iba1 and anti-CD68 antibodies), astrocytes (GFAP) and the anti-CB2R antibody was performed on brain slices from 17-month-old arcAß mice. …”
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A new signature associated with anoikis predicts the outcome and immune infiltration in nasopharyngeal carcinoma by Yonglin Luo, Wenyang Wei, Yaxuan Huang, Jun Li, Weiling Qin, Quanxiang Hao, Jiemei Ye, Zhe Zhang, Yushan Liang, Xue Xiao, Yonglin Cai

“…Key gene expression levels were validated through real-time PCR and immunohistochemical staining. Results Three differentially expressed ARGs (CDC25C, E2F1 and RBL2) with prognostic value were identified by the intersection of multiple machine learning algorithms. …”
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Low-Dose Decitabine Assists Human Umbilical Cord-Derived Mesenchymal Stem Cells in Protecting β Cells via the Modulation of the Macrophage Phenotype in Type 2 Diabetic Mice by Jing Xue, Yu Cheng, Haojie Hao, Jieqing Gao, Yaqi Yin, Songyan Yu, Junyan Zou, Jiejie Liu, Qi Zhang, Yiming Mu

“…The blood glucose and serum insulin levels revealed that DAC prolonged the antidiabetic effect of MSCs to 4 weeks in T2D mice. Immunofluorescence staining demonstrated more sustainable morphological and structural amelioration in islets of the MD group than in the MSC group. …”
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Multi-omics analysis reveals the interplay between intratumoral bacteria and glioma by Ting Li, Zhanyi Zhao, Meichang Peng, Lu Zhang, Cheng Wang, Feiyang Luo, Meiqin Zeng, Kaijian Sun, Zhencheng Fang, Yunhao Luo, Yugu Xie, Cui Lv, Jiaxuan Wang, Jian-Dong Huang, Hongwei Zhou, Haitao Sun

“…We employed immunohistochemistry, multicolor immunofluorescence, and fluorescence in situ hybridization (FISH) staining to observe the presence and location of bacteria. …”
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Lethal necrotizing granulomatous vasculitis after bariatric surgery and bilateral mammaplasty by O. V. Blagova, D. Kh. Aynetdinova, A. V. Nedostup, N. A. Novikova, Ye. A. Kogan, Yu. V. Lerner, L. V. Kozlovskaya, V. V. Rameyev, N. A. Mukhin

“…At transthoracic biopsy of mediastinal lymph nodes demonstrated the pattern of Wegener-type ANCA-negative necrotizing granulomatous vasculitis of small arteries (PCR for mycobacteria and acid fast stain were negative). Despite provided corticosteroid and cyclophosphan treatment, the disease progressed (focal affection of the lungs) and the patient died of brain stem hemorrhage. …”
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Determining the status of tertiary lymphoid structures in invasive pulmonary adenocarcinoma based on chest CT radiomic features by Ye Yu, Tianshu Yang, Pengfei Ma, Yan Zeng, Yongming Dai, Yicheng Fu, Aie Liu, Ying Zhang, Guanglei Zhuang, Yan Zhou, Huawei Wu

“…TLS was divided into two groups according to hematoxylin-eosin staining. Radiomic features were extracted, and support vector machine (SVM) were implemented to predict the status of TLSs. …”
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miR-129-5p Promotes Osteogenic Differentiation of BMSCs and Bone Regeneration via Repressing Dkk3 by Changming Zhao, Yulin Gu, Yan Wang, Qiaozhen Qin, Ting Wang, Meng Huang, Heyang Zhang, Yannv Qu, Jingwen Zhang, Zhangzhen Du, Xiao-Xia Jiang, Lulu Xu

“…The ability of BMSC differentiation to osteoblast was tested by alkaline phosphatase (ALP) and alizarin red staining (ARS). The expression of osteogenic genes (Runx2, Bmp2, and OCN) was examined via quantitative RT-PCR and western blot. …”
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Lower respiratory tract microbiome dysbiosis impairs clinical responses to immune checkpoint blockade in advanced non‐small‐cell lung cancer by Yong Zhang, Xiang‐Xiang Chen, Ruo Chen, Ling Li, Qing Ju, Dan Qiu, Yuan Wang, Peng‐Yu Jing, Ning Chang, Min Wang, Jian Zhang, Zhi‐Nan Chen, Ke Wang

“…Multiplex immunohistochemical staining was then used to assess the immune cell infiltration after immune checkpoint blockade therapy. …”
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The Influence of Overnight Orthokeratology on Ocular Surface and Meibomian Gland Dysfunction in Teenagers with Myopia by Xiu Wang, Jing Li, Rui Zhang, Na Li, Yi Pang, Yan Zhang, Ruihua Wei

“…The corneal fluorescein staining at 1, 3, 6, 12, and 24 months after wearing OOK were all increased than the prewearing level with significant difference (P=0.014, P=0.036, P<0.001, P<0.001, and P=0.008, respectively). …”
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Baccaurea ramiflora fruit peel powder supplementation prevented inflammatory cell infiltration, oxidative stress, and fibrosis in carbon tetrachloride (CCl4) administered ovariecto... by Tahsin Zahan, Mirza Alimullah, Ishrat Jahan, Md. Borhan Uddin, Khondker Ayesha Akter, Md. Junaeid Rahman, Shahnaz Siddiqua, Kamrun Nahar Ela, Sakil Amin, Khondoker Shahin Ahmed, Hemayet Hossain, Ferdous Khan, Md Ashraful Alam, Nusrat Subhan

“…In addition, the process of histological staining of liver sections was done for inflammation as well as tissue fibrosis. …”
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Metasilicate-based alkaline mineral water improves the growth performance of weaned piglets by maintaining gut-liver axis homeostasis through microbiota-mediated secondary bile aci... by Jian Chen, Kanwar K. Malhi, Xiaowei Li, Xiangwen Xu, Jianxun Kang, Bichen Zhao, Yaru Xu, Xuenan Li, Jinlong Li

“…Histopathological evaluations were conducted using hematoxylin and eosin staining. To analyze the composition of the gut microbiota, 16S rRNA PacBio SMRT Gene Full-Length Sequencing was performed. …”
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Development of autonomic innervation at the venous pole of the heart: bridging the gap from mice to human by Fleur Zwanenburg, Thomas A. Bos, Arend D. J. Ten Harkel, Monique C. Haak, Nathan D. Hahurij, Robert E. Poelmann, Conny J. van Munsteren, Lambertus J. Wisse, Nico A. Blom, Marco C. DeRuiter, Monique R. M. Jongbloed

“…Morphological assessment of the venous pole was performed using immunohistochemical stainings for neural crest cells and autonomic nerve markers. …”
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Screening Evaporative Dry Eyes Severity Using an Infrared Image by Qing Zhang, Yi Wu, Yilin Song, Guanghao Qin, Lanting Yang, Sumeet Singh Talwar, Tiezhu Lin, Gagan Deep Singh Talwar, Hongda Zhang, Ling Xu, Jonathan E Moore, Emmanuel Eric Pazo, Wei He

“…Evaporative DED was assessed using noninvasive tear breakup time, fluorescein staining, and the Chinese version of the ocular surface disease index (C-OSDI) questionnaire and categorized into their respective dry eye symptomology group (none, mild, moderate, or severe). …”
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Cell Counts, rather than Proportion, of CD8/PD-1 Tumor-Infiltrating Lymphocytes in a Tumor Microenvironment Associated with Pathological Characteristics of Chinese Invasive Ductal... by Qingkun Song, Feng Shi, Maya Adair, Hong Chang, Xiudong Guan, Yanjie Zhao, Yuchen Li, Guangjiang Wu, Jiangping Wu

“…Immunohistochemistry was used to detect biomarker expression on formalin-fixed and paraffin-embedded sections. Double staining of CD8 and PD-1 was conducted on lymphocytes. …”
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Physiological accumulation of lipid droplets in the newborn liver during breastfeeding is driven by TLR4 ligands by Wanderson Ferreira da Silva Júnior, Karen Marques de Oliveira Costa, Hortência Maciel Castro Oliveira, Maísa Mota Antunes, Kassiana Mafra, Brenda Naemi Nakagaki, Pedro Sérgio Corradi da Silva, Júlia Duarte Megale, Sarah Campos de Sales, Douglas Carvalho Caixeta, Mário Machado Martins, Robinson Sabino-Silva, Cristina Maria Pinto de Paula, Luiz Ricardo Goulart, Rafael Machado Rezende, Gustavo Batista Menezes

“…Using high-resolution microscopy assessed the spatial distribution of lipid droplets within hepatocytes. Lugol's iodine staining determined the timing weaning period, and milk deprivation experiments investigated the relationship between milk intake and fat accumulation. …”
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CASC8 activates the pentose phosphate pathway to inhibit disulfidptosis in pancreatic ductal adenocarcinoma though the c-Myc-GLUT1 axis by Hong-Fei Yao, Jieqiong Ge, Jiahao Chen, Xiaoyan Tang, Chunjing Li, Xiao Hu, Abousalam Abdoulkader Ahmed, Yunlong Pu, Guihua Zhou, Tongyi Zhang, Zhiwei Cai, Chongyi Jiang

“…Methods The existence of disulfidptosis in PDAC was assessed using flow cytometry and F-actin staining. CASC8 expression and its clinical correlations were analyzed using data from The Cancer Genome Atlas (TCGA) and further verified by chromogenic in situ hybridization assay in PDAC tissues. …”
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Mesenchymal stromal cells promote the formation of lung cancer organoids via Kindlin-2 by Zhilin Sui, Xianxian Wu, Jiaxin Wang, ShihJye Tan, Chao Zhao, Zhentao Yu, Chuanyue Wu, Xiaoxiao Wang, Ling Guo

“…We analyzed the morphological and histological similarities between LCOs co-cultured with fibroblast and primary lung cancer lesions through HE and IF staining. We evaluated whether LCOs co-cultured with fibroblast retained the original genetic mutations of their source tumors based on WES. …”
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Helicobacter pylori SlyD stabilizes TPT1 via hnRNPK and enhances OCT1-mediated CDX2 transcriptional activation to drive gastric intestinal metaplasia by Shuwen Zheng, Yingying Wang, Chuxuan Ni, Rui Guo, Xunan Qiu, Jijun Chen, Lu Wang, Xiaohu Sun, Moye Chen, Yunen Liu, Yuan Yuan, Yuehua Gong

“…Immunohistochemistry staining was conducted to validate hnRNPK, TPT1, OCT1, and CDX2 expression in gastric tissue samples from both human and Mongolian gerbils. …”
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