The total extract of Abelmoschus manihot (L.) medic flowers (TEA) mediated Nrf2-TFAM signalling to regulate mitochondrial antioxidant mechanism by Ying Song, Xinyi Zhu, Beibei Wang, Qisong Li, Biwei Song

“…Cell morphology was observed via inverted microscopy, and cell viability was measured via the MTT reagent. The cells were stained with Hoechst 33,324 solution. Reactive oxygen species (ROS), superoxide dismutase (SOD), malondialdehyde (MDA) and ATP detection kits were used to detect the corresponding indicators. …”
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Momordica Charantia Polysaccharides Attenuates MPP+-Induced Injury in Parkinson’s Disease Mice and Cell Models by Regulating TLR4/MyD88/NF-κB Pathway by Dengjun Guo, Jie Zhou, Meng Zhang, Reyisha Taximaimaiti, Xiaoping Wang, Hai Wang

“…Moreover, flow cytometry, PI staining, and fluorescence were used to observe cell apoptosis. …”
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The Effect of A2E on the Ca2+-PKC Signaling Pathway in Human RPE Cells Exposed to Blue Light by Maomei Luo, Shu Wang, Yun Tang, Chun Zeng, Shanjun Cai

“…After blue light exposure, the cells were cultured for 24 h. Fluo-3/AM staining was used to determine the level of cytoplasmic Ca2+, and the cells were photographed using a laser scanning confocal microscope to analyze the fluorescence intensity. …”
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Esketamine at a Clinical Dose Attenuates Cerebral Ischemia/Reperfusion Injury by Inhibiting AKT Signaling Pathway to Facilitate Microglia M2 Polarization and Autophagy by Gao Y, Li L, Zhao F, Cheng Y, Jin M, Xue FS

“…The expression of biomarkers related to microglial M1 and M2 phenotypes in the ischemic brain tissues was determined by immunofluorescence staining and RT-qPCR, and the expression of proteins associated with autophagy and the AKT signaling pathway in the ischemic brain tissues was assayed by Western blotting.Results: Esketamine alone and esketamine combined with rapamycin alleviated neurological impairment, improved sensorimotor function, decreased cerebral infarct volume, and mitigated tissue injury in the MCAO rats. …”
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The Effect of A2E on the Uptake and Release of Calcium in the Lysosomes and Mitochondria of Human RPE Cells Exposed to Blue Light by Mao-Mei Luo, Lin Chen, Shu Wang, Chun Zeng, De-Zhi Li, YeGe Bi, Long-Qian Liu, Shan-Jun Cai

“…Moreover, changes in the mitochondrial membrane potential were detected by flow cytometry analysis of JC-1-stained cells. The obtained results revealed that blue light illumination increased the calcium fluorescence intensity in the cytoplasm, mitochondria, and lysosomes of human RPE cells when compared with the control cells (P<0.05). …”
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Lactoferrin Induces Osteoblast Growth through IGF-1R by Jian-Ming Hou, En-Yu Chen, Fan Lin, Qing-Ming Lin, Ying Xue, Xu-Hua Lan, Man Wu

“…Osteoblasts with silenced IGF-1R exhibited decreased proliferation but increased apoptosis. MMT staining and flow cytometry both indicated that there was no significant difference between the shIGF-1R group and the shIGF-1R + 100 μg/mL lactoferrin group. (3) Lactoferrin (100 μg/mL) induced PI3K and RAS phosphorylation and silence of IGF-1R resulted in decreased p-PI3K and p-RAS expression. …”
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Prevalence of Hyper virulence and Multidrug resistance Klebsiella pneumoniae isolates from UTI patients by Manar Abdul karim, Zainab Mohammed Alzubaidy, Khazal Dabua Wadi

“…Capsule production was also investigated using nigrosin stain followed by direct microscopy examination and it was found that 28 (93.3%) from K. pneumoniae isolates were surrounded by a capsule as well as (100%) biofilm formers. 86.6%, 77% and 70% of the isolates were siderophore, protease and coagulase were giving positive results respectively. …”
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The use of heparin-containing lubricant in clinical practice by T.B. Romanova, M.H. Rabadanova, E.G. Rybakova, Z.U. Teviyeva, M.S. Gorshkova

“…All patients were examined and observed according to a single program, which included: collection of complaints and anamnesis, refractometry and visometry, tonometry, biomicroscopy, staining of the anterior segment of the eye with fluorescein and lissamine green, determination of invasive tear film break-up time and Schirmer test, filling in the McMonnis questionnaire about the dry eye symptoms. …”
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A targeted and synergetic nano-delivery system against Pseudomonas aeruginosa infection for promoting wound healing by Chang Ni, Xisheng Li, Haiye Jiang, Shumin Gui, Heng Yin, Xinmin Nie

“…Flow cytometry, bacterial LIVE/DEAD staining and scanning electron microscopy were performed to assess the in vitro antibacterial and anti-biofilm effects of the nanosystems. …”
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IL-33 Deficiency Attenuates Lung Inflammation by Inducing Th17 Response and Impacting the Th17/Treg Balance in LPS-Induced ARDS Mice via Dendritic Cells by Li Cheng, Yang Jiao, Wei Jiang, Xin Zhang, Liping Zhang, Gongwei Jia

“…Hematoxylin and eosin (H&E) staining, wet/dry (W/D) weight ratios, cell counts, and the levels of tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), interleukin-6 (IL-6), interleukin-17 (IL-17), and interleukin-10 (IL-10) in bronchoalveolar lavage fluid (BALF) were investigated. …”
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The influence of external factors on the development of olfactory disorders in patients with the coronavirus disease 2019: an immunomorphological assessment by I.S. Shponka, O.M. Usova

“…Mucosal samples were stained with standard hematoxylin and eosin and using an immunohistochemical method with antibodies to neuron-specific beta-III tubulin (TuJ-1), olfactory marker protein (OMP) and angiotensin-converting enzyme (ACE-2). …”
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High Glucose Exacerbates TNF-α-Induced Proliferative Inhibition in Human Periodontal Ligament Stem Cells through Upregulation and Activation of TNF Receptor 1 by Wenjun Zhu, Qihong Qiu, Haoyuan Luo, Fuping Zhang, Juan Wu, Xiaorui Zhu, Min Liang

“…Cell apoptosis was assessed by annexin V/PI staining. Protein expression was detected by western blotting. …”
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Tanshinone I Ameliorates Psoriasis-Like Dermatitis by Suppressing Inflammation and Regulating Keratinocyte Differentiation by Wang Q, Luo X, Su Y, Jin Y, Kuang Q, Li S, Shen W, Zhu Y

“…Disease severity was evaluated using the Psoriasis Area and Severity Index (PASI), and histological changes were assessed via H&E staining and Ki67 immunofluorescence. TNF-α-stimulated HaCaT keratinocytes were used for in vitro analyses, including apoptosis, cell cycle progression, and inflammatory gene expression via RT-qPCR. …”
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Identification of CD8+ T Cell-Related Genes: Correlations with Immune Phenotypes and Outcomes of Liver Cancer by Qi Pan, Ying Cheng, Donghua Cheng

“…Using immunohistochemistry and single-cell sequencing analysis, we found that CD3D antibody staining was weaker in tumor tissues than normal tissues and was related to CD8+ T cells. …”
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Phage vB_Kpn_HF0522: Isolation, Characterization, and Therapeutic Potential in Combatting K1 Klebsiella pneumoniae Infections by Yan T, Wang Q, Ma C, Teng X, Gong Z, Chu W, Zhou Q, Liu Z

“…The effect of phage vB_Kpn_HF0522 on biofilms was assessed using crystal violet staining assay. The Galleria mellonella (G. mellonella) infection model and mouse infection models were established to evaluate the practical application potential of the phage and the fitness cost of phage-resistant bacteria.Results: Phage was isolated from hospital sewage for experimental studies. …”
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TLR4 Inhibition Attenuated LPS‐Induced Proinflammatory Signaling and Cytokine Release in Mouse Hearts and Cardiomyocytes by Christine W. Wiger, Trine Ranheim, Henriette Arnesen, Jarle Vaage, Søren E. Pischke, Arne Yndestad, Kåre‐Olav Stensløkken, May‐Kristin Torp

“…Infarct size was quantified by triphenyltetrazolium staining. Cytokine expression was analyzed with ELISA, western blot analysis, and qPCR. …”
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The role of homogenization cycles and Poloxamer 188 on the quality of mitochondria isolated for use in mitochondrial transplantation therapy by Ryosuke Takegawa, Kei Hayashida, Atsushi Murao, Yusuke Endo, Cyrus E. Kuschner, Jacob Kazmi, Eriko Nakamura, Ping Wang, Lance B. Becker

“…MitoTracker Deep Red (MTDR) staining and flow cytometry were used to assess mitochondrial purity, while the JC-1 assay evaluated membrane potential. …”
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Antibacterial Potential of Endemic Medicinal Plants of the Maluku Islands, Indonesia, against Urinary Tract Infection Pathogens by Eka Astuty, Vinsa Delia Luhulima, Stefanus Cahyo Ariwicaksono

“…Following the pathogen isolation, the isolates were identified according to the colony morphology, Gram staining, and biochemical test results. The antibacterial activity was determined through the Kirby-Bauer disc diffusion test. …”
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Gambaran Golongan Sekretor dan Nonsekretor yang Diperiksa Melalui Saliva Mahasiswa Pendidikan Dokter Fakultas Kedokteran Universitas Andalas by Alqadri ., Zelly Dia Rofinda, Rika Susanti

“…Blood type can be checked directly on the scene when there are stains or spots of blood, but in some cases the criminal is usually found saliva in wet or dry form. …”
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Total Plate Count Analysis and Food-Contaminating Bacterial Identification of Smoked Tatihu (Thunnus albacares) Sold in Several Traditional Markets in Ambon, Indonesia by Evangelista Risalia Haurissa, Melda Yunita, Sulfiana Sulfiana

“…The microscopic identification involved Gram staining to determine the shape and color of the bacteria. …”
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