JinQi Jiangtang Tablet Regulates Gut Microbiota and Improve Insulin Sensitivity in Type 2 Diabetes Mice by Ying Cao, Guowang Yao, Yuanyuan Sheng, Li Yang, Zixuan Wang, Zhen Yang, Pengwei Zhuang, Yanjun Zhang

“…D-LA leakage experiment and PAS staining were used to check the function of the gut barrier. …”
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Anti-Inflammatory Effect of a Peptide Derived from the Synbiotics, Fermented Cudrania tricuspidata with Lactobacillus gasseri, on Inflammatory Bowel Disease by Jimyeong Ha, Hyemin Oh, Nam Su Oh, Yeongeun Seo, Joohyun Kang, Min Hee Park, Kyung Su Kim, Shin Ho Kang, Yohan Yoon

“…Large intestine tissue was fixed in 10% formalin and stained with hematoxylin and eosin for histopathological analysis. …”
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Effect of Malaria and Schistosoma mansoni Coinfection on Selected Biochemical Profiles among Patients Attending Selected Health Institutions at Dembiya, Northwest Ethiopia by Wagaw Abebe, Wossenseged Lemma, Yalewayker Tegegne, Amare Mekuanint, Abebe Yenesew, Adane Derso

“…To detect Plasmodium, both thick and thin blood films were prepared and stained with Giemsa. The blood sample was processed for the analysis of biochemical profiles. …”
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In-Situ Electrospinning Dressings Loaded with Kaempferol for Reducing MMP9 to Promote Diabetic Ulcer Healing by Li J, Meng H, Guo W, Zhou L, Wu S, Gao G, Liu Q, You D, Qu W

“…Histological and immunofluorescence staining were performed to determine the status of epithelization, collagen deposition, MMP9 expression, macrophage polarization, inflammation response and angiogenesis.Results: Material science experiments have shown that the dressing has optimal fiber micromorphology and good water vapor transport properties (WVTR: 4.88 kg m− 2 24h− 1); in vivo, diabetic wound experiments have shown that the high porosity and pharmacological effects of PED4 can mutually promote the rapid healing of diabetic wounds (healed 95.9% on day 15), facilitate the transformation of macrophages from M1-type to M2-type and regulate the expression of MMP9.Conclusion: Portable electrospinning dressings equipped with kaempferol not only better fit irregular wounds, but also promote wound healing through MMP9 and macrophage polarization. …”
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p16 expression in patient with Loop Electrosurgical Excision Procedure (LEEP) by Putu Erika Paskarani, I Gede Sastra Winata, Elisa Laura Oktrinita Sitohang, Felyanto Felyanto, Sang Ayu Putu Yuliantini

“…Results: The positive p16 expression was revealed in 12 samples (38.7%), in contrast the negative staining appeared in 19 samples (61.3%). Unfortunately, p16 expression was not significant statistically based on age, parity, and contraceptive history, with p-values of 1.00, 0.45, and 0.65, respectively. …”
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Mechanism of Spica Prunellae in Suppressing Proliferation and Stem Cell Properties of Colorectal Cancer Cells Based on Notch Signaling Pathway by TIAN Haoyuan, XIE Yi, FANG Yi, CHENG Ying, LIN Yan, SU Daifeng, WEI Lihui

“…The number of viable cells was detected by 0.4% Trypan blue staining and cell survival rate was calculated. The proliferation inhibition rate of colorectal cancer cells was detected using Cell Counting Kit-8 (CCK8) assay. …”
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iPSC-derived exosomes as amphotericin B carriers: a promising approach to combat cryptococcal meningitis by Jingyu Zhao, Jingyu Zhao, Wei Fang, Yangjie Gao, Jiquan Chen, Guizhen Wang, Julin Gu

“…The cytotoxicity of the iPSC-Exo/AmB complex was evaluated on HEK 293 T and RAW264.7 cells using the CCK-8 assay, while apoptosis was assessed using live/dead cell staining and flow cytometry. The hemolytic effects were tested using rabbit red blood cells. …”
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Long noncoding RNA Gm4419 promotes mesangial cell proliferation and extracellular matrix accumulation via the miR-455-3p/histone deacetylase 2 axis by Yang Wen, Hua Gan, Qing Zhong, Ying Gong

“…Cell counting kit-8 assay and 5-ethynyl-2′-deoxyuridine staining assay were adopted to assess cell proliferation. …”
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Cutaneous Anthrax: What is the Hallmark? by Dhani Redhono Harioputro, Arie Kusumawardani, Iin Novita, Evi Nurhayatun, Rahajeng N Tunjungputri, Erni Juwita Nelwan

“…The anti-anthrax protective antigen (anti-PA) IgG level is found to be seropositive with a level of 85 U/ml. Gram staining of the tissue underneath the eschar found Gram-positive rod bacteria in reddish-purple color. …”
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Metformin Ameliorates Ulcerative Colitis Through Inhibiting NLRP3 Inflammasome Activation by Cao R, Jing J, Ma Y, Qi W, Huang X, Zhang C, Lu Z, He J, Wang G, Ma Y, Zhang H

“…Disease Activity Index (DAI) and hematoxylin and eosin staining (HE) were performed to evaluate the impact of Met on UC model. …”
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Curdione combined with borneol treats bacterial mixed HPV infection by regulating the crosstalk among immune cells by Jingwei Liu, Jingwei Liu, Tong Shu, Yiheng Mu, Wanlin Zheng, Xiaohuan Lu, Xiaohuan Lu, Hong Tao, Hong Tao

“…Bioinformatics and network pharmacology approaches were employed to identify therapeutic targets of Baofukang suppository for BV/HPV co-infections. qRT-PCR, Western blot, immunofluorescence staining, and flow cytometry were utilized to validate the therapeutic targets of curdione and borneol, along with the associated immune molecular changes. …”
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Novel role of microphthalmia-associated transcription factor in modulating the differentiation and immunosuppressive functions of myeloid-derived suppressor cells by Yoon Kyung Jeon, Aram Lee, Haesun Park, Soyoung Lim, Jihyun Lim, Jaemoon Koh, Young Yang, Myeong-Sok Lee, Jong-Seok Lim

“…While increased MITF expression in MDSCs promoted breast cancer progression and CD4+ or CD8+ T-cell dysfunction, decreased MITF expression in MDSCs suppressed tumor progression and enhanced T-cell activation. Furthermore, IHC staining of human tumor tissues revealed that MITF+ MO-MDSCs are more frequently observed in tumor tissues than in tumor-free draining lymph nodes obtained from patients with cancer.Conclusions Our results indicate that MITF regulates the differentiation and function of MDSCs and can be a novel therapeutic target for modulating MDSC activity in immunosuppressive TMEs.…”
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Reproductive problems in water buffalo cows associated with non-diagnosed genital lepto- spirosis. Preliminary results by Rosaura Pérez-Gil

“…In addition, tissue samples were prepared for histological analysis by fixation in a 10% buffered formaldehyde solution and stained with hematoxylin and eosin. PCR was performed on nine culture samples for identification of Leptospira spp., by using two pairs of primer for the markers, G1-G2 derived from a sequence from the genomic library of L. interrogans serovar icterohaemorragiae, strain RGA, which was described by Gravekamp et al. (1993) and Internal 1 and Internal 2 derived from the sequence of the gene encoding the LipL32 protein and specific to identify pathogenic Leptospira, described by Haake et al. (2000). …”
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Murine DX5+NKT Cells Display Their Cytotoxic and Proapoptotic Potentials against Colitis-Inducing CD4+CD62Lhigh T Cells through Fas Ligand by Jens M. Werner, Michael Damian, Stefan A. Farkas, Hans J. Schlitt, Edward K. Geissler, Matthias Hornung

“…After polyclonal stimulation with anti-CD3, anti-CD28, and IL-2, proliferation of CD4+CD62Lhigh cells was assessed using a CFSE assay and activity of proapoptotic caspase-3 was determined by intracellular staining and flow cytometry. Extrinsic apoptotic pathway was blocked using an unconjugated antibody against FasL, and activation of caspase-3 was measured. …”
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Hirudo extract ameliorates proliferative vitreoretinopathy by promoting autophagy and attenuating the THBS2/PI3K/Akt pathway by Hui Huang, Qun Huang, Jing Wang, Wanjie Wang, Yanlin Zheng

“…CCK-8, wound healing, and Transwell assays were performed to detect the viability, migration, and invasion of the cells. Immunofluorescence staining was used to detect F-actin expression. Autophagy was observed via transmission electron microscopy. …”
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Giant Granular Cell Tumor of the Left Thigh, a Rare Case Report and Literature Review by Liu Q, Kong X, Yang J, Zhang D

“…Immunohistochemistry demonstrated positive staining for S100, SOX-10, Vimentin, NSE, CD56, and H3K27Me3 in the tumor cells, with a Ki-67 labeling index of approximately 15%. …”
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Correlation between METTL3 overexpression and 18F-FDG uptake in patients with soft tissue sarcoma by Tong Wu, Jinghui Xie, Hongbo Feng, Hua Zhang, Juan Tao, Bo Chen

“…Immunohistochemical staining and 18F-FDG radioautography were performed on tumor tissues from 39 patients with STS undergoing 18F-FDG PET before treatment. …”
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The role of endothelial cell-related gene COL1A1 in prostate cancer diagnosis and immunotherapy: insights from machine learning and single-cell analysis by Gujun Cong, Jingjing Shao, Feng Xiao, Haixia Zhu, Peipei Kang

“…The XGBoost and Random Forest algorithms highlighted the significant role of COL1A1, and we further analyzed the expression and correlation of COL1A1, AR, and EGFR through multiplex immunofluorescence staining. In vitro experimental analysis of the impact of COL1A1 on the progression of PRAD. …”
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Surface Roughness and Color Stability of Conventional and Bulk-Fill Resin Composite with S-PRG Fillers After Coffee Exposure: An in-vitro Study by Janisch FADS, Falcon Aguilar M, Aguiar FHB, França FMG, Basting RT, Vieira-Junior WF

“…Considering the S-PRG materials, the bulk-fill version is more susceptible to staining.Keywords: composite resins, coffee, color, surface properties…”
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Aminoguanidine hemisulfate improves mitochondrial autophagy, oxidative stress, and muscle force in Duchenne muscular dystrophy via the AKT/FOXO1 pathway in mdx mice by Shiyue Sun, Tongtong Yu, Joo Young Huh, Yujie Cai, Somy Yoon, Hafiz Muhammad Ahmad Javaid

“…Gene expression, western blotting, H&E staining, immunofluorescence, ROS assays, TUNEL apoptosis, glutathione activity, and muscle force measurements were performed. …”
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