High, but variable prevalence of Sarcocystis cruzi infections in farm-raised American bison (Bison bison) beef destined for human consumption by Larissa S. de Araujo, Aditya Gupta, Marianne Dias Papadopoulos, Doaa Naguib, Jacquin Battle, Oliver Kwok, Asis Khan, Benjamin Rosenthal, Jitender P. Dubey

“…Sarcocysts were detected in histological sections stained with hematoxylin and eosin in 167 of 200 samples. …”
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Study of the neuroprotective properties of metformin in rats with type 2 diabetes mellitus and brain injury induced by intracerebral hemorrhage by V.L. Holubiev, M.H. Oberemok, V.A. Tkachenko, Yu.V. Kharchenko, O.O. Bondarenko, A.E. Lievykh, V.I. Zhyliuk

“…Histopathological examination was performed using general histological staining techniques and immunohistochemical methods for assessment of expression of endothelial NO-synthase (eNOS), Growth Associated Protein 43 (GAP43), Hypoxia-inducible factor 1-alpha (HIF-1α), neural cadherine (N-cadherine) and vascular endothelial cadherine (VE-cadherine). …”
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A Study of Trichomonas vaginalis Infection and Correlates in Women with Vaginal Discharge Referred at Fann Teaching Hospital in Senegal by Roger C. Tine, Khadime Sylla, Rougyatou Ka, Lamine Dia, Doudou Sow, Souleye Lelo, Khardiata Diallo, Babacar Faye, Thérèse Dieng, Cheikh T. Ndour, Ahmet Y. Sow

“…Optic microscopic examination with 40x magnification was done to detect T. vaginalis and assess biological modifications such as presence of epithelial cells, white blood cells, and red blood cells. A gram stained smear was also performed and examined under oil immersion (100x magnification) to assess the vaginal flora. …”
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The Effect of Blood-Derived Products on the Chondrogenic and Osteogenic Differentiation Potential of Adipose-Derived Mesenchymal Stem Cells Originated from Three Different Location... by Markus Neubauer, Olga Kuten, Christoph Stotter, Karina Kramer, Andrea De Luna, Thomas Muellner, Zsombor Lacza, Stefan Nehrer

“…The viability of the cells was determined by XTT assay, and the progress of differentiation was tested via histological staining and monitoring of specific gene expression. …”
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Hydrogen Sulfide Promotes Functional Endometrium Recovery via Regulating Pyroptosis in Severe Endometrial Injury: A Prospective Laboratory Based Randomized Control Trial by Haiyan Shan, Mingyuan Xu, Jie Ni, Ling Zhou, Mengchun Xue, Jun Xue, Xiaohuan Yuan, Luyang Tao, Mingyang Zhang, Hong Zhang

“…Western blot analysis was conducted to assess changes in the expression of endogenous H2S metabolism and pyroptosis-related markers and histological analysis (Hematoxylin & Eosin and Masson staining) was employed to examine alterations in endometrial morphology. …”
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Enhancement of systemic and lung-localized CD4⁺ T-cell immune responses by truncation of NS1 protein of a seasonal live influenza vaccine strain by Polina I. Prokopenko, Ekaterina A. Stepanova, Victoria A. Matyushenko, Alexandra Ya. Rak, Anna K. Chistyakova, Arina D. Kostromitina, Tatyana S. Kotomina, Igor V. Kudryavtsev, Artem A. Rubinstein, Alexey S. Komlev, Larisa G. Rudenko, Irina N. Isakova-Sivak

“…Levels of systemic and tissue-resident cytokine-producing CD4+ and CD8+ memory T cells were assessed by intracellular cytokine staining assay with flow cytometry. Replication of engineered vaccine strains in in vitro and in vivo systems was also evaluated. …”
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Effect of Bacille Calmette–Guérin vaccination on immune responses to SARS‐CoV‐2 and COVID‐19 vaccination by Nicole L Messina, Susie Germano, Amy W Chung, Carolien E van deSandt, Natalie E Stevens, Lilith F Allen, Rhian Bonnici, Julio Croda, Claudio Counoupas, Branka Grubor‐Bauk, Ebene R Haycroft, Katherine Kedzierska, Ellie McDonald, Rebecca McElroy, Mihai G Netea, Boris Novakovic, Kirsten P Perrett, Laure F Pittet, Ruth A Purcell, Kanta Subbarao, James A Triccas, David J Lynn, Nigel Curtis, the BRACE Trial Consortium Group

“…SARS‐CoV‐2‐specific antibodies were measured using ELISA and multiplex bead array, whole blood cytokine responses to γ‐irradiated SARS‐CoV‐2 (iSARS) stimulation were measured by multiplex bead array, and SARS‐CoV‐2‐specific T‐cell responses were measured by activation‐induced marker and intracellular cytokine staining assays. Results After randomisation (mean 11 months) but prior to COVID‐19 vaccination, the BCG group had lower cytokine responses to iSARS stimulation than the Control group. …”
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AutoLDP: An accurate and efficient artificial intelligence-based tool for automatic labeling of digital pathology by Yingnan Zhao, Huifen Ye, Jing Yang, Su Yao, Maohua Lv, Zhihong Chen, Yunrui Ye, Qingru Hu, Cheng Lu, Zaiyi Liu, Ke Zhao, Zhihua Chen

“…The naming format is divided into four parts: pathology ID, wax block ID, staining type, and slice type. We used the accuracy and processing time to validate our method using two validation sets. …”
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Maternal exposure to deltamethrin during pregnancy and lactation impairs hippocampal learning and memory function of male offspring by ferroptosis by Shasha Huang, Wanqi Li, Dengke Wang, Huiwen Feng, Bo Wang, Xinyu Dong, Wei Zhao, Dan Liu, Yuan Wang

“…We used behavioral experiments and Nissl staining to observe the hippocampal development and learning and memory function of male offspring. …”
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Icariin modulates osteogenic and adipogenic differentiation in ADSCs via the Hippo-YAP/TAZ pathway: a novel therapeutic strategy for osteoporosis by Shaozi Lin, Zuyu Meng, Mei Wang, Zixuan Ye, Mengsha Long, Yiyao Zhang, Fang Liu, Hongling Chen, Menghan Li, Jiajia Qin, Haiquan Liu

“…Osteogenic and adipogenic differentiation were assessed using Alizarin Red and Oil Red O staining, respectively. Third-generation ADSCs were divided into five groups: control, resveratrol (100 μmol/L), and four ICA treatment groups (1, 10, 50, and 100 μmol/L). …”
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Reduced Expression of Antimicrobial Protein Secretory Leukoprotease Inhibitor and Clusterin in Chronic Rhinosinusitis with Nasal Polyps by Yanran Huang, Ming Wang, Yu Hong, Xiangting Bu, Ge Luan, Yang Wang, Ying Li, Hongfei Lou, Chengshuo Wang, Luo Zhang

“…SLPI is expressed in both nasal epithelial cells and glandular cells, whereas CLU is mainly expressed in glandular cells. AB/PAS staining further demonstrated that both SLPI and CLU were mainly produced by mucous cells in submucosal glands. …”
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Electroacupuncture Improves Clearance of Amyloid-β through the Glymphatic System in the SAMP8 Mouse Model of Alzheimer’s Disease by Pei-zhe Liang, Li Li, Ya-nan Zhang, Yan Shen, Li-li Zhang, Jie Zhou, Zhi-jie Wang, Shu Wang, Sha Yang

“…After EA treatment, Morris water maze (MWM) test was used to evaluate the cognitive function; HE and Nissl staining was performed to observe the brain histomorphology; ELISA, contrast-enhanced MRI, and immunofluorescence were applied to explore the mechanisms underlying EA effects from Aβ accumulation, glymphatic system function, reactivity of astrocytes, and AQP4 polarization, respectively. …”
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Scoring System for Lesions Induced by Different Strains of Newcastle Disease Virus in Chicken by Elawad A. Hussein, M. Hair-Bejo, Lawan Adamu, A. R. Omar, Siti S. Arshad, Elmutaz A. Awad, I. Aini

“…In all previous experiments, tissues of brain, trachea, lung, caecal tonsil, liver, kidney, spleen, heart, proventriculus, intestine, and thymus were collected, fixed in 10% buffered formalin, embedded in paraffin, and sectioned. HS staining was applied. Tissues were examined under light microscope and changes were recorded. …”
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PD-L1 positive platelets mediate resistance to immune checkpoint inhibitors in patients with colorectal cancer by Jiacheng Li, Jia Liu, Shifeng Yang, Yu Xia, Qingzhe Meng, Biying Sun, Yansong Liu, Bin Zhao, Jiaqi Jin, Hui Xu, Lihong Wang, Pengxia Zhang, Zhuoxin Cheng

“…Methods The functional depletion effects of PD-L1 positive platelets on TME and immune cells were measured via western blotting, immunofluorescence staining, qRT-PCR, ELISpot and flow cytometry. In vivo, CD274 knockout (KO), CD8a KO, platelet-specific KO (PF4-Cre-Hsp90b1flox/flox) mouse models and a subcutaneous tumour model treated with aspirin and PD-L1 mAb were established in C57BL/6 N mice. …”
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Jianpi Yiqi Busui prescription alleviates myasthenia gravis by regulating Th17 through the TAK1/P38 MAPK/eIF-4E signaling pathway by Zhuming Chen, Jing Lu, Tianying Chang, Dongmei Zhang, Yibin Zhang, Miao Liu, Tong Wu, Peng Xv, Jian Wang

“…Western blot and immunofluorescence staining were used to detect the expression levels of key proteins and their phosphorylated forms, such as transforming growth factor beta-activated kinase 1 (TAK1), P38 mitogen-activated protein kinase (P38 MAPK), and eukaryotic initiation factor 4E (eIF-4E). …”
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Aqueous extracts of Moringa oleifera and Cinnamomum cassia as promising sources of antibiofilm compounds against mucoid and small colony variants of Pseudomonas aeruginosa and Stap... by Eduarda Silva, Pedro Ferreira-Santos, José A. Teixeira, Maria Olivia Pereira, Cristina M.R. Rocha, Ana Margarida Sousa

“…These extracts were evaluated for extraction yield, antioxidant activity, phenolic content, chemical composition by HPLC-TOF-MS, and antibiofilm activity using a 96-well plate assay, followed by crystal violet staining, bacterial adhesion assessment, and brightfield microscopy. …”
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Adipose-derived stem cells promote the recovery of intestinal barrier function by inhibiting the p38 MAPK signaling pathway by Mei Yang, Wangbin Xu, Chaofu Yue, Rong Li, Xian Huang, Yongjun Yan, Qinyong Yan, Shisheng Liu, Yuan Liu, Qiaolin Li

“…The homing effect of ADSCs was detected by fluorescence labeling, and intestinal barrier tissue was observed by HE staining. After ADSC treatment, the level of phosphorylated p38 MAPK protein decreased, the expression of inflammatory factors, oxidative stress and cell apoptosis decreased, the expression of tight junction proteins increased, and cell permeability decreased in Caco-2 cells stimulated with LPS. …”
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GC-derived exosomal circMAN1A2 promotes cancer progression and suppresses T-cell antitumour immunity by inhibiting FBXW11-mediated SFPQ degradation by Yikai Shen, Jie Lin, Tianlu Jiang, Xusheng Shen, Ying Li, Yiwang Fu, Penghui Xu, Lang Fang, Zetian Chen, Hongxin Huang, Yiwen Xia, Zekuan Xu, Linjun Wang

“…The biological function of circMAN1A2 in GC was investigated using a series of in vitro and in vivo experiments. PKH-67 staining was used to label the exosomes. The molecular mechanism of exosomal circMAN1A2 was investigated via mass spectrometry, immunoprecipitation, Western blot, and single-cell RNA-sequencing data analyses. …”
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Polydatin protects against DSS-induced ulcerative colitis via Nrf2/Slc7a11/Gpx4-dependent inhibition of ferroptosis signalling activation by Shimin Zheng, Jianbin Yin, Bingbing Wang, Qiujuan Ye, Jialuo Huang, Xinzhi Liang, Junfeng Wu, Hui Yue, Ting Zhang

“…Ferroptosis-related proteins Slc7a11, Nrf2 and Gpx4 levels were measured by western blot, immunohistochemical and immunofluorescence staining.ResultsPD mitigated the DSS-induced increases in pro-inflammatory cytokines (IL-6, TNF-α, and IL-1β), alleviated colon length shortening, reduced morphological damage to the intestinal mucosa, and preserved tight junction proteins (TJ) occludin and Zonula occludens-1 (ZO-1) in both caco-2 cells and murine models of colitis. …”
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Identification of a distinctive immunogenomic gene signature in stage-matched colorectal cancer by Pankaj Ahluwalia, Ashis K. Mondal, Ashutosh Vashisht, Harmanpreet Singh, Ahmet Alptekin, Kalyani Ballur, Nivin Omar, Meenakshi Ahluwalia, Kimya Jones, Amanda Barrett, Vamsi Kota, Ravindra Kolhe

“…To differentiate the tumor and adjacent normal regions (NAT), H&E staining was utilized. For the quantification of transcripts, we used the NanoString nCounter platform. …”
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